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The map label for this gene is topB [H]

Identifier: 167624556

GI number: 167624556

Start: 3195843

End: 3197879

Strand: Reverse

Name: topB [H]

Synonym: Shal_2636

Alternate gene names: 167624556

Gene position: 3197879-3195843 (Counterclockwise)

Preceding gene: 167624557

Following gene: 167624555

Centisome position: 61.18

GC content: 47.37

Gene sequence:

>2037_bases
ATGATCTTATATATTGCTGAAAAACCTAGTCTTGGTAGAGCCATTGCCGATGTATTGCCTAAGCCTTTAAAGAAAGGTGA
CGGGTTTATTCGCGCTGCAAATGGCGATTGTGTCTCTTGGTGCGTCGGCCATCTACTTGAGCAAGCTGAGCCTGATGCTT
ATTCGCCTGAATACAAGTCATGGAAGTTTGAACATCTACCGATCGTGCCTGATAACTGGAAGATGAAACCCAAATCTAAG
ATGCGTGGCCAGATTGCAGTACTTAGAGGCCTAGTTAAAGAGGCGACTCAATTAGTGAATGCTGGCGATCCAGATAGAGA
GGGGCAGCTTTTGGTCGATGAGATCATCGCCCATCTCGGGGTTAAAGGGGCTAAGTTACAAAATGCGCAGCGCTTGTTGA
TCAGCGATTTAAATCCTCAAGCAGTAAAAAGAGCATTAGGACAGTTGCGCTCAAACCGCGAATTTATTCCCCTTTCGACT
TCAGCGCTTGCGCGTTCCCGCGCCGATTGGCTCTATGGTATGAACATGACCCGTGCTTATACCATCCAAGGCCGTAAGGT
GGGTTACCAAGGCGTGCTGTCTGTTGGGCGGGTGCAAACCCCGGTGCTGGGCTTAGTGGTGCGCCGCGACGGTGAAATTG
CCGAGTTTGTCTCTAAACCTTTTTATGAAGTGTTTGCGCATCTGGCTACCGACAACCAAGAGCGCTTTAGCGCCAAATGG
CAACCGAGTGAAGCCTGTCTGCCCTATATGGATGAAGAGGGGCGGGTACTATCAAAAGGGTTGGCGCAAAATGTGGTGGG
TCGCATTAACGGCCAACCTGCCACGGTAGAAAAGGTAACCGCTCAAGATAAGAAGCAGAATGCGCCTTTGCCTTATAGCT
TATCGGCACTGCAGATTGATGCCGCTAAGCGCTTTGGTATGAGCGCAAAAGCTGTGCTCGATACCTGTCAGTCGTTATAT
GAGAAGCACAAACTTATCACTTATCCCCGCTCAGATAGTCGTTACCTGCCAGTTGATCAACACGGGCTAGCCCCAAGTGT
TATTCGCGCGGTGCTTAGCGGCGCGAGTGAATTGGTGTCTAGTTGCGATGAGCCTAATGCCAAACTTAAGTCTAAGGCGT
GGAACGACAAGAAAGTCGACGCCCACCATGCGATAGTGCCTACCGAAAAAACAGCTAATTTATCTAACCTTAGCCAGGCT
GAGAAGCAGTTATATCTGCAAATTGCACGTCAGTACTTGATGCAGTTTTATCCGGCTTATCTTTATAAAGAGACCCAAGT
TGAGATCATCATTGCTGGTGGTTTATTTAAAACTAAGGCTAAGCAAGAGGTGGCGTTAGGTTGGAAGCAACTCCTTAAGC
TGTCAGCAAAAGCAAGCCAAGAAAAGAGTTCGAGTGAAAAGTCACAAACTGATGATGACGAGCATCTAGCTAATATTCCA
GCCCTCAAAAAAGGCCAAATTCTGCTGTGTGAACGCGGTGAGTTATTAGAGAAAAATACCCAACCACCAAAGCACTTTAC
CGATGCGACATTACTGGGCGCGATGACCGGGATCAGTCGTTATGTTACCGATAGTGAAATTCGTAAAATCTTAAAAGACA
CCGACGGCTTAGGGACTGAAGCGACGCGGGCTGGAATTATTGAGCTGTTATTTAAGCGTAACTATTTAACTCGTCAAGGT
AAGTCTATTTTGGCCACAGAGGTGGGTAAAGGCTTAATTTACAGCTTACCCCAAAGTGCGACGACGCCGGATATGACAGC
ACTTTGGGAAAATAACCTCGATGCCATAAGCCGTAAAGAGGCTAAATATCAGAGTTTTATGGACCCTTTGGTCAACTCTC
TGCAAGGGTTGATTGAACAAGCGGGCTCGCAACTGCCCACATCATTGCAAGGGGTTAAGTCTCCAGATGGCGGCTTTAAA
AAACGTCGCTATTCCGGCAAAAAGAGTACGACCACGACAAAATCGGCTATCGCCAATAAAACACCAGCTAAAAGAGCGAG
CACGGCTATGACTAAGACAAAGAAAGCGACTTCCTAA

Upstream 100 bases:

>100_bases
TCGCTGGTAAATAGCCTCGACCGTTCGGCTTTCATTTGCACCCTTTAGCGCTTCAAGGTACAGTTACTGTTAATTTATAC
AGTAATCCTAATTCTTGCTG

Downstream 100 bases:

>100_bases
ATCATCAGCTTGAGTTCTTGTTTAGTAACTTTCAATTGTCTACCGCAGCCCTGTAGGTTAACTTTGTTGCTCAGTATAGT
AATGACTGAGTATGCCAATG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKSWKFEHLPIVPDNWKMKPKSK
MRGQIAVLRGLVKEATQLVNAGDPDREGQLLVDEIIAHLGVKGAKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGRKVGYQGVLSVGRVQTPVLGLVVRRDGEIAEFVSKPFYEVFAHLATDNQERFSAKW
QPSEACLPYMDEEGRVLSKGLAQNVVGRINGQPATVEKVTAQDKKQNAPLPYSLSALQIDAAKRFGMSAKAVLDTCQSLY
EKHKLITYPRSDSRYLPVDQHGLAPSVIRAVLSGASELVSSCDEPNAKLKSKAWNDKKVDAHHAIVPTEKTANLSNLSQA
EKQLYLQIARQYLMQFYPAYLYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKLSAKASQEKSSSEKSQTDDDEHLANIP
ALKKGQILLCERGELLEKNTQPPKHFTDATLLGAMTGISRYVTDSEIRKILKDTDGLGTEATRAGIIELLFKRNYLTRQG
KSILATEVGKGLIYSLPQSATTPDMTALWENNLDAISRKEAKYQSFMDPLVNSLQGLIEQAGSQLPTSLQGVKSPDGGFK
KRRYSGKKSTTTTKSAIANKTPAKRASTAMTKTKKATS

Sequences:

>Translated_678_residues
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKSWKFEHLPIVPDNWKMKPKSK
MRGQIAVLRGLVKEATQLVNAGDPDREGQLLVDEIIAHLGVKGAKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGRKVGYQGVLSVGRVQTPVLGLVVRRDGEIAEFVSKPFYEVFAHLATDNQERFSAKW
QPSEACLPYMDEEGRVLSKGLAQNVVGRINGQPATVEKVTAQDKKQNAPLPYSLSALQIDAAKRFGMSAKAVLDTCQSLY
EKHKLITYPRSDSRYLPVDQHGLAPSVIRAVLSGASELVSSCDEPNAKLKSKAWNDKKVDAHHAIVPTEKTANLSNLSQA
EKQLYLQIARQYLMQFYPAYLYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKLSAKASQEKSSSEKSQTDDDEHLANIP
ALKKGQILLCERGELLEKNTQPPKHFTDATLLGAMTGISRYVTDSEIRKILKDTDGLGTEATRAGIIELLFKRNYLTRQG
KSILATEVGKGLIYSLPQSATTPDMTALWENNLDAISRKEAKYQSFMDPLVNSLQGLIEQAGSQLPTSLQGVKSPDGGFK
KRRYSGKKSTTTTKSAIANKTPAKRASTAMTKTKKATS
>Mature_678_residues
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKSWKFEHLPIVPDNWKMKPKSK
MRGQIAVLRGLVKEATQLVNAGDPDREGQLLVDEIIAHLGVKGAKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGRKVGYQGVLSVGRVQTPVLGLVVRRDGEIAEFVSKPFYEVFAHLATDNQERFSAKW
QPSEACLPYMDEEGRVLSKGLAQNVVGRINGQPATVEKVTAQDKKQNAPLPYSLSALQIDAAKRFGMSAKAVLDTCQSLY
EKHKLITYPRSDSRYLPVDQHGLAPSVIRAVLSGASELVSSCDEPNAKLKSKAWNDKKVDAHHAIVPTEKTANLSNLSQA
EKQLYLQIARQYLMQFYPAYLYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKLSAKASQEKSSSEKSQTDDDEHLANIP
ALKKGQILLCERGELLEKNTQPPKHFTDATLLGAMTGISRYVTDSEIRKILKDTDGLGTEATRAGIIELLFKRNYLTRQG
KSILATEVGKGLIYSLPQSATTPDMTALWENNLDAISRKEAKYQSFMDPLVNSLQGLIEQAGSQLPTSLQGVKSPDGGFK
KRRYSGKKSTTTTKSAIANKTPAKRASTAMTKTKKATS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1788061, Length=664, Percent_Identity=54.6686746987952, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=562, Percent_Identity=23.6654804270463, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74799; Mature: 74799

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKS
CEEEEECCCCHHHHHHHHCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPDNWKMKPKSKMRGQIAVLRGLVKEATQLVNAGDPDREGQLLVDEIIAHLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
VKGAKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGRKVGYQGVLSVGRVQTPVLGLVVRRDGEIAEFVSKPFYEVFAHLATDNQERFSAKW
EECCCCCCHHHHHHHCCHHCCHHEEEEECCCHHHHHHHCHHHHHHHHHHCCCHHHHCCCC
QPSEACLPYMDEEGRVLSKGLAQNVVGRINGQPATVEKVTAQDKKQNAPLPYSLSALQID
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHEEEH
AAKRFGMSAKAVLDTCQSLYEKHKLITYPRSDSRYLPVDQHGLAPSVIRAVLSGASELVS
HHHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHH
SCDEPNAKLKSKAWNDKKVDAHHAIVPTEKTANLSNLSQAEKQLYLQIARQYLMQFYPAY
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKLSAKASQEKSSSEKSQTDDDEHLANIP
HCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
ALKKGQILLCERGELLEKNTQPPKHFTDATLLGAMTGISRYVTDSEIRKILKDTDGLGTE
CCCCCCEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCH
ATRAGIIELLFKRNYLTRQGKSILATEVGKGLIYSLPQSATTPDMTALWENNLDAISRKE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCHHHHHHHH
AKYQSFMDPLVNSLQGLIEQAGSQLPTSLQGVKSPDGGFKKRRYSGKKSTTTTKSAIANK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHCC
TPAKRASTAMTKTKKATS
CCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILYIAEKPSLGRAIADVLPKPLKKGDGFIRAANGDCVSWCVGHLLEQAEPDAYSPEYKS
CEEEEECCCCHHHHHHHHCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPDNWKMKPKSKMRGQIAVLRGLVKEATQLVNAGDPDREGQLLVDEIIAHLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
VKGAKLQNAQRLLISDLNPQAVKRALGQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGRKVGYQGVLSVGRVQTPVLGLVVRRDGEIAEFVSKPFYEVFAHLATDNQERFSAKW
EECCCCCCHHHHHHHCCHHCCHHEEEEECCCHHHHHHHCHHHHHHHHHHCCCHHHHCCCC
QPSEACLPYMDEEGRVLSKGLAQNVVGRINGQPATVEKVTAQDKKQNAPLPYSLSALQID
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHEEEH
AAKRFGMSAKAVLDTCQSLYEKHKLITYPRSDSRYLPVDQHGLAPSVIRAVLSGASELVS
HHHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHH
SCDEPNAKLKSKAWNDKKVDAHHAIVPTEKTANLSNLSQAEKQLYLQIARQYLMQFYPAY
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYKETQVEIIIAGGLFKTKAKQEVALGWKQLLKLSAKASQEKSSSEKSQTDDDEHLANIP
HCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
ALKKGQILLCERGELLEKNTQPPKHFTDATLLGAMTGISRYVTDSEIRKILKDTDGLGTE
CCCCCCEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCH
ATRAGIIELLFKRNYLTRQGKSILATEVGKGLIYSLPQSATTPDMTALWENNLDAISRKE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCHHHHHHHH
AKYQSFMDPLVNSLQGLIEQAGSQLPTSLQGVKSPDGGFKKRRYSGKKSTTTTKSAIANK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHCC
TPAKRASTAMTKTKKATS
CCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]