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The map label for this gene is estA [H]

Identifier: 167624314

GI number: 167624314

Start: 2892622

End: 2893302

Strand: Reverse

Name: estA [H]

Synonym: Shal_2391

Alternate gene names: 167624314

Gene position: 2893302-2892622 (Counterclockwise)

Preceding gene: 167624316

Following gene: 167624313

Centisome position: 55.35

GC content: 48.16

Gene sequence:

>681_bases
ATGTCTGACTCCAAGCTTGAGCGTATTACGATAGAACCAACCCAAGAGGCAAAAGCCTGCGTTATCTGGCTTCATGGCCT
AGGAGACTCTGGTGCAGGTTTTGCTCCTGTTGTACCAGCATTAGGCTTAGGTAGTGAGCATGGGATCCGCTTTATATTCC
CTCATGCGCCAGAGCAAGCCGTCACCATTAACGGTGGCTATATTATGCGTGCTTGGTATGACATTAAGAGTATGGATCTA
CATGAGCGAGCGGATAAGCAAGGTGTCGAGCAGAGTGAGCAACAGATCATCGCTTTGATTGAAGAACAAATAGCGCTCGG
GATCCCAACTGAAAACATTGTGTTAGCCGGCTTTAGTCAAGGCGGGGTGATGAGCCTGCACACAGGCCTTCGGTTACCGC
ATAAGCTGGCTGGGATAATGGCGCTTTCTTGCTACCTTCCAAGTGGTGACTCCCTCCCCAAAGGACTTTCAAATGTAAAT
CGTGACACGTCAATCATACAGCACCACGGTGTTGAAGATGATGTAGTTCCAGTATATGCAGGTGAAATGGCCTTTAAACT
ACTGCAAGGCGAGGGGTTTAATACCCAATGGAAAACTTACAACATGGCCCACAACGTTTTGCCCGAGCAGCTACAAGATA
TTTCGCTTTGGCTGCAATCAGTGCTGCCATACGGTAAGTAA

Upstream 100 bases:

>100_bases
TTACCGTCGCGGTTCTTTAAGGGGCGGCACTATGGGCTAACTCCCTATAGGCTGGTAATATCAAGGCGAATTTAACAAAT
AGTCATTTTTAAGGGATACA

Downstream 100 bases:

>100_bases
AATCTATTTGCTTGTTTTTTGCACAGACTGGCCGCGATACTTTCGTTGCCAGCTGTTCCGTAATCGGGATAATTAACGGC
ATTCCTTCTTGGCTTTAACT

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSDSKLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSEHGIRFIFPHAPEQAVTINGGYIMRAWYDIKSMDL
HERADKQGVEQSEQQIIALIEEQIALGIPTENIVLAGFSQGGVMSLHTGLRLPHKLAGIMALSCYLPSGDSLPKGLSNVN
RDTSIIQHHGVEDDVVPVYAGEMAFKLLQGEGFNTQWKTYNMAHNVLPEQLQDISLWLQSVLPYGK

Sequences:

>Translated_226_residues
MSDSKLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSEHGIRFIFPHAPEQAVTINGGYIMRAWYDIKSMDL
HERADKQGVEQSEQQIIALIEEQIALGIPTENIVLAGFSQGGVMSLHTGLRLPHKLAGIMALSCYLPSGDSLPKGLSNVN
RDTSIIQHHGVEDDVVPVYAGEMAFKLLQGEGFNTQWKTYNMAHNVLPEQLQDISLWLQSVLPYGK
>Mature_225_residues
SDSKLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSEHGIRFIFPHAPEQAVTINGGYIMRAWYDIKSMDLH
ERADKQGVEQSEQQIIALIEEQIALGIPTENIVLAGFSQGGVMSLHTGLRLPHKLAGIMALSCYLPSGDSLPKGLSNVNR
DTSIIQHHGVEDDVVPVYAGEMAFKLLQGEGFNTQWKTYNMAHNVLPEQLQDISLWLQSVLPYGK

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=217, Percent_Identity=39.63133640553, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI9966764, Length=217, Percent_Identity=36.8663594470046, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI20270341, Length=227, Percent_Identity=29.0748898678414, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17508057, Length=212, Percent_Identity=34.9056603773585, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323147, Length=215, Percent_Identity=35.8139534883721, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553055, Length=216, Percent_Identity=34.7222222222222, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45553057, Length=216, Percent_Identity=34.7222222222222, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21357257, Length=216, Percent_Identity=34.7222222222222, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI161078179, Length=220, Percent_Identity=33.6363636363636, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24697; Mature: 24565

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSKLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSEHGIRFIFPHAPEQA
CCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCE
VTINGGYIMRAWYDIKSMDLHERADKQGVEQSEQQIIALIEEQIALGIPTENIVLAGFSQ
EEECCCEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGVMSLHTGLRLPHKLAGIMALSCYLPSGDSLPKGLSNVNRDTSIIQHHGVEDDVVPVYA
CCEEEECCCCCCHHHHHHHHEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCCCCCEEEEH
GEMAFKLLQGEGFNTQWKTYNMAHNVLPEQLQDISLWLQSVLPYGK
HHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDSKLERITIEPTQEAKACVIWLHGLGDSGAGFAPVVPALGLGSEHGIRFIFPHAPEQA
CCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCE
VTINGGYIMRAWYDIKSMDLHERADKQGVEQSEQQIIALIEEQIALGIPTENIVLAGFSQ
EEECCCEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGVMSLHTGLRLPHKLAGIMALSCYLPSGDSLPKGLSNVNRDTSIIQHHGVEDDVVPVYA
CCEEEECCCCCCHHHHHHHHEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCCCCCEEEEH
GEMAFKLLQGEGFNTQWKTYNMAHNVLPEQLQDISLWLQSVLPYGK
HHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA