Click here to switch to the map view.

The map label for this gene is pyrD

Identifier: 167624280

GI number: 167624280

Start: 2847205

End: 2848224

Strand: Reverse

Name: pyrD

Synonym: Shal_2356

Alternate gene names: 167624280

Gene position: 2848224-2847205 (Counterclockwise)

Preceding gene: 167624281

Following gene: 167624279

Centisome position: 54.49

GC content: 39.61

Gene sequence:

>1020_bases
ATGTTTTATAAAATCGCACAAAAGTTCATGTTTCAGATGGATCCAGAGCTGGCACATAATTTAGCTATCGGCAGTTTAAA
GTCTACTGGTAATTCACCTTTAAACTGCTTTTATGCGCAAAATATTAAGCCAGCCCCTGTTACTATGATGGGCCTGACTT
TTCCAAATCCTGTAGGTCTTGCTGCCGGTATGGATAAAGACGGTGAGTGTATCGATGCATTTCATGCTATGGGGTTTGGT
CATATTGAAGTGGGAACAGTCACCCCGCGTCCACAACCAGGTAATGATAAGCCACGCTGTTTTAGACTTAAGCCTGCTAA
AGCAATTATTAATCGCATGGGATTCAACAATAAAGGTGTAGACAACCTAGTTGAAAACCTCAAAGCTGTTAAGACAAATG
CGATGGTTGGTGTCAATATCGGTAAGAATAAAGATACGCCAGTAGAGCAGGGCAAAGATGATTATTTAATCTGCATGGAT
AAGGTATATCCTTACGCCGCCTATATTGCAGTAAATATTTCTTCGCCAAACACGCCTGGATTGCGATCGCTTCAGTATGG
CGATCTGCTCGATGATCTTCTTGGATCATTAAAACAAAAGCAAAAAGAACTCGCTGAAAAACATGGTAAGTACGTGCCAA
TTGCATTAAAAATTGCACCTGACTTATCAAGTGAAGAGATTGAAAAGATAGCTGAGGCACTAATAAAGAGTGAATTTGAC
GCTGCTATTGCGACAAATACTACACTAACTCGTGATGGTGTTAGCGGTTTATTGAATGCTAATGAAGCTGGTGGGTTAAG
TGGTAAGCCGTTGAATTCATTGTCAACTATAGTGATTAAGCAGTTAGCTGATTGCTTGAAAGGTAAGTTACCAATCATTG
GTGTCGGTGGTATTAACGCCGCTGAAGATGCGTTAGATAAATTAGATGCGGGTGCTGAGATGGTTCAGATTTTTTCTGGG
TTTATTTATCAAGGACCTAAATTAATTAAAGATATCGTCGAAGCATATCGAATTAAATAA

Upstream 100 bases:

>100_bases
AACTTAATCTTTTGATCCTTCATTGTGAAGGCCAAAAGTAATCTGATTTAAATCAGAAGCCCTGGCAAGTGCCGGGGCTT
TTTTCGGTCTAGGAGAAAAC

Downstream 100 bases:

>100_bases
TCTAGTTTAGATCTAATATTTTTATTAGATCATAAAACGATCATTCAGATCTCAAAAGGATCTTGGTGATCGTTTTTTAT
TTAGATATTAGATTTGATTT

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MFYKIAQKFMFQMDPELAHNLAIGSLKSTGNSPLNCFYAQNIKPAPVTMMGLTFPNPVGLAAGMDKDGECIDAFHAMGFG
HIEVGTVTPRPQPGNDKPRCFRLKPAKAIINRMGFNNKGVDNLVENLKAVKTNAMVGVNIGKNKDTPVEQGKDDYLICMD
KVYPYAAYIAVNISSPNTPGLRSLQYGDLLDDLLGSLKQKQKELAEKHGKYVPIALKIAPDLSSEEIEKIAEALIKSEFD
AAIATNTTLTRDGVSGLLNANEAGGLSGKPLNSLSTIVIKQLADCLKGKLPIIGVGGINAAEDALDKLDAGAEMVQIFSG
FIYQGPKLIKDIVEAYRIK

Sequences:

>Translated_339_residues
MFYKIAQKFMFQMDPELAHNLAIGSLKSTGNSPLNCFYAQNIKPAPVTMMGLTFPNPVGLAAGMDKDGECIDAFHAMGFG
HIEVGTVTPRPQPGNDKPRCFRLKPAKAIINRMGFNNKGVDNLVENLKAVKTNAMVGVNIGKNKDTPVEQGKDDYLICMD
KVYPYAAYIAVNISSPNTPGLRSLQYGDLLDDLLGSLKQKQKELAEKHGKYVPIALKIAPDLSSEEIEKIAEALIKSEFD
AAIATNTTLTRDGVSGLLNANEAGGLSGKPLNSLSTIVIKQLADCLKGKLPIIGVGGINAAEDALDKLDAGAEMVQIFSG
FIYQGPKLIKDIVEAYRIK
>Mature_339_residues
MFYKIAQKFMFQMDPELAHNLAIGSLKSTGNSPLNCFYAQNIKPAPVTMMGLTFPNPVGLAAGMDKDGECIDAFHAMGFG
HIEVGTVTPRPQPGNDKPRCFRLKPAKAIINRMGFNNKGVDNLVENLKAVKTNAMVGVNIGKNKDTPVEQGKDDYLICMD
KVYPYAAYIAVNISSPNTPGLRSLQYGDLLDDLLGSLKQKQKELAEKHGKYVPIALKIAPDLSSEEIEKIAEALIKSEFD
AAIATNTTLTRDGVSGLLNANEAGGLSGKPLNSLSTIVIKQLADCLKGKLPIIGVGGINAAEDALDKLDAGAEMVQIFSG
FIYQGPKLIKDIVEAYRIK

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=329, Percent_Identity=42.8571428571429, Blast_Score=245, Evalue=4e-65,
Organism=Escherichia coli, GI1787177, Length=333, Percent_Identity=60.960960960961, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17509475, Length=351, Percent_Identity=39.031339031339, Blast_Score=227, Evalue=8e-60,
Organism=Drosophila melanogaster, GI281361352, Length=326, Percent_Identity=41.4110429447853, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI17137316, Length=326, Percent_Identity=41.4110429447853, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_SHEHH (B0TIY0)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674574.1
- ProteinModelPortal:   B0TIY0
- SMR:   B0TIY0
- GeneID:   5902672
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2356
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- ProtClustDB:   PRK05286
- BioCyc:   SHAL458817:SHAL_2356-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 36530; Mature: 36530

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYKIAQKFMFQMDPELAHNLAIGSLKSTGNSPLNCFYAQNIKPAPVTMMGLTFPNPVGL
CHHHHHHHHHHHCCHHHHHHHHHCCHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCC
AAGMDKDGECIDAFHAMGFGHIEVGTVTPRPQPGNDKPRCFRLKPAKAIINRMGFNNKGV
CCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCH
DNLVENLKAVKTNAMVGVNIGKNKDTPVEQGKDDYLICMDKVYPYAAYIAVNISSPNTPG
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCC
LRSLQYGDLLDDLLGSLKQKQKELAEKHGKYVPIALKIAPDLSSEEIEKIAEALIKSEFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
AAIATNTTLTRDGVSGLLNANEAGGLSGKPLNSLSTIVIKQLADCLKGKLPIIGVGGINA
HHHHCCCEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
AEDALDKLDAGAEMVQIFSGFIYQGPKLIKDIVEAYRIK
HHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYKIAQKFMFQMDPELAHNLAIGSLKSTGNSPLNCFYAQNIKPAPVTMMGLTFPNPVGL
CHHHHHHHHHHHCCHHHHHHHHHCCHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCC
AAGMDKDGECIDAFHAMGFGHIEVGTVTPRPQPGNDKPRCFRLKPAKAIINRMGFNNKGV
CCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCH
DNLVENLKAVKTNAMVGVNIGKNKDTPVEQGKDDYLICMDKVYPYAAYIAVNISSPNTPG
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCC
LRSLQYGDLLDDLLGSLKQKQKELAEKHGKYVPIALKIAPDLSSEEIEKIAEALIKSEFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
AAIATNTTLTRDGVSGLLNANEAGGLSGKPLNSLSTIVIKQLADCLKGKLPIIGVGGINA
HHHHCCCEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
AEDALDKLDAGAEMVQIFSGFIYQGPKLIKDIVEAYRIK
HHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA