Click here to switch to the map view.

The map label for this gene is dapE

Identifier: 167624234

GI number: 167624234

Start: 2794655

End: 2795800

Strand: Direct

Name: dapE

Synonym: Shal_2310

Alternate gene names: 167624234

Gene position: 2794655-2795800 (Clockwise)

Preceding gene: 167624233

Following gene: 167624235

Centisome position: 53.47

GC content: 46.16

Gene sequence:

>1146_bases
ATGAGCCAAAATACAGACTCTCCAGTACTTTCATTAGCCAAAGACCTTATTTCTCGTCAATCGGTCACACCTTTAGATGA
AGGTTGCCAGCAACTCATGGCTGACAGATTAGCCGACGCAGGCTTTAATATTGAGTCAATGGTATTTGAAGATACCACCA
ATATGTGGGCTCGCAGAGGCACGCAATCTCCCGTCTTTTGTTTTGCAGGCCATACCGATGTTGTCCCAGTTGGAGATCTA
AATCGTTGGCACACACCACCGTTTGAGCCAGTTGTTATTGATGATTATCTTCATGGGCGCGGCGCTGCAGATATGAAAGG
CTCATTGGCGGCGATGCTGGTGGCTACCGAGCGCTTCATTAAAAAGTTTCCGGATCATCAAGGCTCAATCGCCTTTCTGA
TCACCAGTGATGAGGAAGGCCCTTTTATTAATGGCACAACTCGAGTCATCGATACCCTTGAAGCACGTAACGAAAAAATC
ACCTGGTCGCTCGTGGGAGAACCTTCATCCACCCATAAGCTAGGTGATATCGTTAAGAATGGGCGCCGAGGCAGTCTCAC
AGGTAATTTAACGGTTAAAGGAGTTCAAGGGCACGTGGCTTACCCACACCTTGCTGATAACCCTATTCATAAAGCCGCTC
CTGCGCTAGATGAGCTTGCGAGAATGAAATGGGATAATGGTAATGAGTTTTTCCCGCCGACTAGCTTTCAAATCGCAAAT
ATCAACGGCGGTACAGGCGCATCAAACGTTATACCAGGTGCTTTAGAGGTGATGTTTAATTTTCGCTACTCCACCGAAGT
TACCGCCGAAATATTAATAGAGCGAGTGCTCAATATTCTCGATGCTCACGGCTTAGAGTACGATATCGATTGGGTCTTCA
ATGGCTTGCCTTTTCTAACTGGCGATGGACCTTTATTAGAAGCAACTAAAGCTGCAATCAAAAAAGTTACTGGAACGAAT
ACCGATCCACAAACATCGGGTGGCACGTCAGATGGTCGTTTTATTGCTCCAACCGGGGCACAGGTTATTGAGCTTGGCCC
TGTTAATGCTACCATTCATAAAGTGAACGAGTGTGTCAAAGTATCAGACCTTGAGTTACTCACCGATTGTTATGAAGCCA
TTTTGGAAAACCTGCTGTGCAAGTAG

Upstream 100 bases:

>100_bases
TGGCACTATACCCAACCTTGATCAAGCGCCCCGTTTTAGCGATGAATGGACAAGTCCAGGTGGGTTTTAAAGAAGCTGAC
TATCGAACGTGGTTCAACCT

Downstream 100 bases:

>100_bases
AACATCCCTTTCTATACGGCCTTGAGGATCAACACTTAGTCGAATGCTTAGGATACCTGTTAGAAGCAAACACTGCGGCT
GCATTTTTTAACATGCAGCA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSQNTDSPVLSLAKDLISRQSVTPLDEGCQQLMADRLADAGFNIESMVFEDTTNMWARRGTQSPVFCFAGHTDVVPVGDL
NRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFIKKFPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWSLVGEPSSTHKLGDIVKNGRRGSLTGNLTVKGVQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLEYDIDWVFNGLPFLTGDGPLLEATKAAIKKVTGTN
TDPQTSGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVKVSDLELLTDCYEAILENLLCK

Sequences:

>Translated_381_residues
MSQNTDSPVLSLAKDLISRQSVTPLDEGCQQLMADRLADAGFNIESMVFEDTTNMWARRGTQSPVFCFAGHTDVVPVGDL
NRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFIKKFPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWSLVGEPSSTHKLGDIVKNGRRGSLTGNLTVKGVQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLEYDIDWVFNGLPFLTGDGPLLEATKAAIKKVTGTN
TDPQTSGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVKVSDLELLTDCYEAILENLLCK
>Mature_380_residues
SQNTDSPVLSLAKDLISRQSVTPLDEGCQQLMADRLADAGFNIESMVFEDTTNMWARRGTQSPVFCFAGHTDVVPVGDLN
RWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFIKKFPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKIT
WSLVGEPSSTHKLGDIVKNGRRGSLTGNLTVKGVQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIANI
NGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLEYDIDWVFNGLPFLTGDGPLLEATKAAIKKVTGTNT
DPQTSGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVKVSDLELLTDCYEAILENLLCK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=373, Percent_Identity=59.2493297587131, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1790395, Length=278, Percent_Identity=28.0575539568345, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17551016, Length=264, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_SHEHH (B0TVI4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674528.1
- ProteinModelPortal:   B0TVI4
- SMR:   B0TVI4
- GeneID:   5906242
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2310
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   SHAL458817:SHAL_2310-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41377; Mature: 41246

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 73-73 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTDSPVLSLAKDLISRQSVTPLDEGCQQLMADRLADAGFNIESMVFEDTTNMWARRG
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHCC
TQSPVFCFAGHTDVVPVGDLNRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFI
CCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
KKFPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWSLVGEPSSTHKLGDIVKN
HHCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GRRGSLTGNLTVKGVQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
CCCCCEEECEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLEYDIDWVFNGLPFLT
CCCCCCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEE
GDGPLLEATKAAIKKVTGTNTDPQTSGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHH
VSDLELLTDCYEAILENLLCK
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQNTDSPVLSLAKDLISRQSVTPLDEGCQQLMADRLADAGFNIESMVFEDTTNMWARRG
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHCC
TQSPVFCFAGHTDVVPVGDLNRWHTPPFEPVVIDDYLHGRGAADMKGSLAAMLVATERFI
CCCCEEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
KKFPDHQGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWSLVGEPSSTHKLGDIVKN
HHCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
GRRGSLTGNLTVKGVQGHVAYPHLADNPIHKAAPALDELARMKWDNGNEFFPPTSFQIAN
CCCCCEEECEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
INGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVLNILDAHGLEYDIDWVFNGLPFLT
CCCCCCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEE
GDGPLLEATKAAIKKVTGTNTDPQTSGGTSDGRFIAPTGAQVIELGPVNATIHKVNECVK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHH
VSDLELLTDCYEAILENLLCK
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA