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The map label for this gene is hypF [H]

Identifier: 167624190

GI number: 167624190

Start: 2749250

End: 2751637

Strand: Direct

Name: hypF [H]

Synonym: Shal_2266

Alternate gene names: 167624190

Gene position: 2749250-2751637 (Clockwise)

Preceding gene: 167624189

Following gene: 167624191

Centisome position: 52.6

GC content: 46.27

Gene sequence:

>2388_bases
ATGACAAAAAATCTGGAAAAGAGTCGTTTACAGCTAAAGATCACCGGCATCGTTCAAGGTGTCGGTTTTCGTCCTTTTGT
ATATCGGTTAGCCCATGAGCTCAATCTAACCGGAACCGTACTGAATAATAGTGAAGGAGTGACAATTGAGGCTCAGGGGC
GCCCTGCTGAACTTGCTTTGTTTGAAGCTGCGTTTAGCAACCAAGCGCCTCCACTCGCGCGTATTGACAATATTAATGTC
ACCCCGCTGCCGGTTATCGATGACTGCGATCTGTTTAGTATCGTTCATAGTGAAAAAGAGAGTCTTGCCGTTGTTGCCGT
ATCATCTGACAAATGCACCTGCGAAGACTGTTTTAAAGATATCAACGATATTAATAACCGCCACTTTCGATACCCGTTCA
CCAATTGCACTAATTGTGGACCTAGGTATTCCCTGATCAATGCCCTGCCTTATGATAGACCTAATACTGCGATGGCTGAT
TTTGACATGTGCCCCGACTGTGAGCGTGCTTATTTAGACCCGATGGACAGGCGTTACCACGCACAACCTGTGAGCTGCCC
CAAGTGCGGCCCTCAATTGAGCTTCAAAAGTGCCGATCTGACTCTGCTCAGTAGTAAAGAGACTGCCCTTGAGGATGCGA
TTAATTCTCTCATCGAAGGTAAAATATTAGCCATTAAAGGCATTGGTGGCTTTCATCTAGTCTGTGATGCCACTAACAAT
CAGAGTGTTGAACTGTTACGACAGCGCAAGAATCGCCCAGCGAAACCGTTTGCGGTAATGGTTGAAAATATCGACGCCGC
TAAGAGACTCGTTACAGGTAACACGGCTGAATGGCAGGTCCTAAGCAGCGCCGAGCGCCCTATTACCTTGATGCGCAAAA
TCGATGCTCACGATGTCGAGCCATCGCTAAGTGATCTTGTTGCACCTCAGATAGACAGACTCGGGATCTTCCTGCCTTAT
ACGCCTTTGCACCAACTCTTACTAAATGGGGTTAAACGTCCCTTAGTCATGACCAGTGCAAACCTCTCGGGTGAACCAAT
CATCACCGATAGCGTGGCAATTAATTGCAAGCTGGCTAATGTCATCGATAATATTCTCGATCACAACCGTCCGATATTGA
ATGGCTGTGACGACAGTGTCGTGCAAGTCATAAATGACAAACTACAGGTACTACGTTTAGCAAGGGGGTACGCGCCGCTA
TCTTTCTATAGCGAGCAGCCACTGGAAGAAAGCATTCTTGCGCTGGGTGCCCAACAGAAAAACAGTATCTGTTTTGGTAT
TGAGCACAACCTATTCCTAAGCCCTCATATTGGCGATCTGGTGTCAATTGAAGCCGAGGAGTATTTCCACCATACACTCA
ATACGTTCTCAAGACTTTATGGGTTTAAGGCCAGTCAATTAGTCCATGATAGCCACCCAGATTATGCAACGAGTAGGTGG
GCCTTGTCGCAAGCCATTAACAATACTAGTTCGGTGCAACATCACTACGCACATGTTTTGAGCGTCATGGCCGCAAACAA
GATCACCACGCCCGTACTGGGTTTTAGTTTTGATGGTACAGGGTTAGGCGATGACGATACGCTTTGGGGCGGAGAACTGT
TGCGCTGTGATGTTCACCAATATCAGCGTCTTTCCCACTTGAGTACCTTTGCATTGATTGGTGGCGAACAAGCTATCAAG
CAACCAGTTAGAATTTTACTGTCTATTTTGCTTGAAAAACATTCACCAGAAGTAATCTATAAGCTCGATATTCCTGCGTT
AAAGGCACTATCACCGACGATGTTTACTAACCTAGTTAAGCTTTGGCAAACGAATACGAGTATTAAAACGTCATCGATTG
GAAGATTGTTTGATGCAGTAGCTGTTGCTCTTGGGATGATTAGTGATACTCAGTATGAAGGACAAGCCGGGATCTTAATT
GAAACCGCAGCCAACTCACTGAGCTCAGCTTCTGAACTGAATCATTTTGATGGCCAATTTAAGCTTCAACCGCTACTTTG
CCCGGCCCCAGAAACAATAGCTGAGCACCATGAGCCCATTAGGTTTGATATAAAGCAGTCAAACGGCCAATGGGATAGCT
CAGAACTGCTAGGGCAAATTGTAGAGCAAATTACCCTGATGCCGCTCACGCCTCTTCGTGCAGCACTCATTGCCAAAGCG
TTTATGGACGCTCTGTGCAATTTGGTCTGTGAATGTGCCAAGGATCATCTTGATACCCCTATCGTACTTTGCGGCGGAGT
ATTTCAAAATCGATATCTACTTGAGCGTTGTGAGAAACAACTTAACGCACAGGGCAATCAGCTACTTTCGAGCAACAAGG
TGCCGATAAACGATGCTGGCATTGCACTCGGCCAACTGTGGTATGCAATGCACGACAAACATGTGTGA

Upstream 100 bases:

>100_bases
TTCACCCTTAATGCTAATTCGAAGATGGATCTTGATGGTCTAAATATCGCTGCTGGTGCTTTGTACCTTAAACAGCGTAG
ACACTAAGCAACTGTAACTC

Downstream 100 bases:

>100_bases
CAGGCATCACCCAACAACAAACACAAATCTGATTTGCATCAAGGCCACAACTGTCGGCCTAGTTGACAATTAACTTAAAC
AAAAATAACGGAGTTACCTA

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MTKNLEKSRLQLKITGIVQGVGFRPFVYRLAHELNLTGTVLNNSEGVTIEAQGRPAELALFEAAFSNQAPPLARIDNINV
TPLPVIDDCDLFSIVHSEKESLAVVAVSSDKCTCEDCFKDINDINNRHFRYPFTNCTNCGPRYSLINALPYDRPNTAMAD
FDMCPDCERAYLDPMDRRYHAQPVSCPKCGPQLSFKSADLTLLSSKETALEDAINSLIEGKILAIKGIGGFHLVCDATNN
QSVELLRQRKNRPAKPFAVMVENIDAAKRLVTGNTAEWQVLSSAERPITLMRKIDAHDVEPSLSDLVAPQIDRLGIFLPY
TPLHQLLLNGVKRPLVMTSANLSGEPIITDSVAINCKLANVIDNILDHNRPILNGCDDSVVQVINDKLQVLRLARGYAPL
SFYSEQPLEESILALGAQQKNSICFGIEHNLFLSPHIGDLVSIEAEEYFHHTLNTFSRLYGFKASQLVHDSHPDYATSRW
ALSQAINNTSSVQHHYAHVLSVMAANKITTPVLGFSFDGTGLGDDDTLWGGELLRCDVHQYQRLSHLSTFALIGGEQAIK
QPVRILLSILLEKHSPEVIYKLDIPALKALSPTMFTNLVKLWQTNTSIKTSSIGRLFDAVAVALGMISDTQYEGQAGILI
ETAANSLSSASELNHFDGQFKLQPLLCPAPETIAEHHEPIRFDIKQSNGQWDSSELLGQIVEQITLMPLTPLRAALIAKA
FMDALCNLVCECAKDHLDTPIVLCGGVFQNRYLLERCEKQLNAQGNQLLSSNKVPINDAGIALGQLWYAMHDKHV

Sequences:

>Translated_795_residues
MTKNLEKSRLQLKITGIVQGVGFRPFVYRLAHELNLTGTVLNNSEGVTIEAQGRPAELALFEAAFSNQAPPLARIDNINV
TPLPVIDDCDLFSIVHSEKESLAVVAVSSDKCTCEDCFKDINDINNRHFRYPFTNCTNCGPRYSLINALPYDRPNTAMAD
FDMCPDCERAYLDPMDRRYHAQPVSCPKCGPQLSFKSADLTLLSSKETALEDAINSLIEGKILAIKGIGGFHLVCDATNN
QSVELLRQRKNRPAKPFAVMVENIDAAKRLVTGNTAEWQVLSSAERPITLMRKIDAHDVEPSLSDLVAPQIDRLGIFLPY
TPLHQLLLNGVKRPLVMTSANLSGEPIITDSVAINCKLANVIDNILDHNRPILNGCDDSVVQVINDKLQVLRLARGYAPL
SFYSEQPLEESILALGAQQKNSICFGIEHNLFLSPHIGDLVSIEAEEYFHHTLNTFSRLYGFKASQLVHDSHPDYATSRW
ALSQAINNTSSVQHHYAHVLSVMAANKITTPVLGFSFDGTGLGDDDTLWGGELLRCDVHQYQRLSHLSTFALIGGEQAIK
QPVRILLSILLEKHSPEVIYKLDIPALKALSPTMFTNLVKLWQTNTSIKTSSIGRLFDAVAVALGMISDTQYEGQAGILI
ETAANSLSSASELNHFDGQFKLQPLLCPAPETIAEHHEPIRFDIKQSNGQWDSSELLGQIVEQITLMPLTPLRAALIAKA
FMDALCNLVCECAKDHLDTPIVLCGGVFQNRYLLERCEKQLNAQGNQLLSSNKVPINDAGIALGQLWYAMHDKHV
>Mature_794_residues
TKNLEKSRLQLKITGIVQGVGFRPFVYRLAHELNLTGTVLNNSEGVTIEAQGRPAELALFEAAFSNQAPPLARIDNINVT
PLPVIDDCDLFSIVHSEKESLAVVAVSSDKCTCEDCFKDINDINNRHFRYPFTNCTNCGPRYSLINALPYDRPNTAMADF
DMCPDCERAYLDPMDRRYHAQPVSCPKCGPQLSFKSADLTLLSSKETALEDAINSLIEGKILAIKGIGGFHLVCDATNNQ
SVELLRQRKNRPAKPFAVMVENIDAAKRLVTGNTAEWQVLSSAERPITLMRKIDAHDVEPSLSDLVAPQIDRLGIFLPYT
PLHQLLLNGVKRPLVMTSANLSGEPIITDSVAINCKLANVIDNILDHNRPILNGCDDSVVQVINDKLQVLRLARGYAPLS
FYSEQPLEESILALGAQQKNSICFGIEHNLFLSPHIGDLVSIEAEEYFHHTLNTFSRLYGFKASQLVHDSHPDYATSRWA
LSQAINNTSSVQHHYAHVLSVMAANKITTPVLGFSFDGTGLGDDDTLWGGELLRCDVHQYQRLSHLSTFALIGGEQAIKQ
PVRILLSILLEKHSPEVIYKLDIPALKALSPTMFTNLVKLWQTNTSIKTSSIGRLFDAVAVALGMISDTQYEGQAGILIE
TAANSLSSASELNHFDGQFKLQPLLCPAPETIAEHHEPIRFDIKQSNGQWDSSELLGQIVEQITLMPLTPLRAALIAKAF
MDALCNLVCECAKDHLDTPIVLCGGVFQNRYLLERCEKQLNAQGNQLLSSNKVPINDAGIALGQLWYAMHDKHV

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=791, Percent_Identity=35.6510745891277, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 87815; Mature: 87684

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLEKSRLQLKITGIVQGVGFRPFVYRLAHELNLTGTVLNNSEGVTIEAQGRPAELAL
CCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHH
FEAAFSNQAPPLARIDNINVTPLPVIDDCDLFSIVHSEKESLAVVAVSSDKCTCEDCFKD
HHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
INDINNRHFRYPFTNCTNCGPRYSLINALPYDRPNTAMADFDMCPDCERAYLDPMDRRYH
HHHCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCC
AQPVSCPKCGPQLSFKSADLTLLSSKETALEDAINSLIEGKILAIKGIGGFHLVCDATNN
CCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCC
QSVELLRQRKNRPAKPFAVMVENIDAAKRLVTGNTAEWQVLSSAERPITLMRKIDAHDVE
CHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHCCCCCCC
PSLSDLVAPQIDRLGIFLPYTPLHQLLLNGVKRPLVMTSANLSGEPIITDSVAINCKLAN
CCHHHHHCCCHHHCEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCEEECCEEEEEEHHH
VIDNILDHNRPILNGCDDSVVQVINDKLQVLRLARGYAPLSFYSEQPLEESILALGAQQK
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHCCCCC
NSICFGIEHNLFLSPHIGDLVSIEAEEYFHHTLNTFSRLYGFKASQLVHDSHPDYATSRW
CCEEEEECCCEEECCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
ALSQAINNTSSVQHHYAHVLSVMAANKITTPVLGFSFDGTGLGDDDTLWGGELLRCDVHQ
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHEEECHHH
YQRLSHLSTFALIGGEQAIKQPVRILLSILLEKHSPEVIYKLDIPALKALSPTMFTNLVK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHH
LWQTNTSIKTSSIGRLFDAVAVALGMISDTQYEGQAGILIETAANSLSSASELNHFDGQF
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCE
KLQPLLCPAPETIAEHHEPIRFDIKQSNGQWDSSELLGQIVEQITLMPLTPLRAALIAKA
EEEEEECCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FMDALCNLVCECAKDHLDTPIVLCGGVFQNRYLLERCEKQLNAQGNQLLSSNKVPINDAG
HHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHH
IALGQLWYAMHDKHV
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKNLEKSRLQLKITGIVQGVGFRPFVYRLAHELNLTGTVLNNSEGVTIEAQGRPAELAL
CCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHH
FEAAFSNQAPPLARIDNINVTPLPVIDDCDLFSIVHSEKESLAVVAVSSDKCTCEDCFKD
HHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
INDINNRHFRYPFTNCTNCGPRYSLINALPYDRPNTAMADFDMCPDCERAYLDPMDRRYH
HHHCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCC
AQPVSCPKCGPQLSFKSADLTLLSSKETALEDAINSLIEGKILAIKGIGGFHLVCDATNN
CCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCC
QSVELLRQRKNRPAKPFAVMVENIDAAKRLVTGNTAEWQVLSSAERPITLMRKIDAHDVE
CHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHCCCCCCC
PSLSDLVAPQIDRLGIFLPYTPLHQLLLNGVKRPLVMTSANLSGEPIITDSVAINCKLAN
CCHHHHHCCCHHHCEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCEEECCEEEEEEHHH
VIDNILDHNRPILNGCDDSVVQVINDKLQVLRLARGYAPLSFYSEQPLEESILALGAQQK
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHCCCCC
NSICFGIEHNLFLSPHIGDLVSIEAEEYFHHTLNTFSRLYGFKASQLVHDSHPDYATSRW
CCEEEEECCCEEECCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
ALSQAINNTSSVQHHYAHVLSVMAANKITTPVLGFSFDGTGLGDDDTLWGGELLRCDVHQ
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHEEECHHH
YQRLSHLSTFALIGGEQAIKQPVRILLSILLEKHSPEVIYKLDIPALKALSPTMFTNLVK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHH
LWQTNTSIKTSSIGRLFDAVAVALGMISDTQYEGQAGILIETAANSLSSASELNHFDGQF
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCE
KLQPLLCPAPETIAEHHEPIRFDIKQSNGQWDSSELLGQIVEQITLMPLTPLRAALIAKA
EEEEEECCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FMDALCNLVCECAKDHLDTPIVLCGGVFQNRYLLERCEKQLNAQGNQLLSSNKVPINDAG
HHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHH
IALGQLWYAMHDKHV
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]