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The map label for this gene is nemA [H]

Identifier: 167624028

GI number: 167624028

Start: 2536378

End: 2537481

Strand: Direct

Name: nemA [H]

Synonym: Shal_2104

Alternate gene names: 167624028

Gene position: 2536378-2537481 (Clockwise)

Preceding gene: 167624027

Following gene: 167624029

Centisome position: 48.53

GC content: 43.93

Gene sequence:

>1104_bases
ATGATAAATAACCAATGTATACAAGCCAAACAAAAATTACTTAGCCCATTGAAGCTAGGTGAGATAATCCTCAATAATCG
CGTTGTTATGGCGCCAATGACTCGAAATCGAGCTAACAGTGACGGTACACCCACTCAAAGTATGGTTCAACATTATGCGG
ATAGAGCATCTGCGGGGTTAATTGTTGCTGAAGGGACCTGGCCTAGCCATTCTGGCCAAGCATATAGTAGGCAGCCAGGT
ATTGAGACCAAAGCGCATTGTCAGGCGTGGCGCCATGTCACTGATGCTGTGCATGCTAAAGGTGGCAAAATCGTGTTGCA
AATAATGCACAGTGGACGGATTGGCAGTAAGCACATAAAAGGTGAAGCGGTTAACAGTGTCGCGCCTTCTGCAATACAAG
CCGCAGGACAAGTTTATACCGACAGCGCAGGCATGCAAGACTTTGATATGCCGCTAGCCCTCAGCTCTGTTCAGATTGCA
AAAGTTATAGCTGAGCATCGACAAGCTGCAATCAATGCCATTGAGGCGGGTTTTGATGGAGTAGAACTTCACTGCACTAG
CGGTTATTTACCCATGCAGTTTCTATGTTCCGGAACGAACCAGCGTACTGATAAATATGGCGGTAGTAGCGATAAACGTG
CAAGTTTTGCTTACGAATGTTTATTAAGTATGAGTGATGCCATAGGCGCAGGGCGTGTTGGCTTAAGAATTAATCCGGGA
AATAGCTTTAACGATTGTTCTGATGACGATCCAGCACAAAGCCATATCAGTTTACTAAAGCAAGTTAAAAGCTTAAACCT
TGCGTATTTACATGTGATGAAATCACCGTTAACTGACATTGATGCATTTGCGCTAGCGCGTGAGCATTTTAACGGACCGC
TAATTTTAAACGACGGCTTTGAACCTAGCAGTGCTGCACAAGCGGTACAAGATGAGCAAGGTCAAGCGGTGTCTTTTGCC
AGACATTTTATTGCCAACCCTGATTTAGTTTACCGAATTAATGATGACCTACCGTTATCAAAATTTGATCGGAAAAGCTT
ATATACCGCGGGAGATGCAGGCTACAACGATTATCAATCTCATCAAATAATAACCACTAGTTAA

Upstream 100 bases:

>100_bases
TTTTTGGCGAGTGATGATGCTTCATATGTTACAGGCGTGAGCTTACCCGTTGATGGTGGTCGCTCGGCAGTACTCGTAAC
GGCCTTGGATTGAGGTAGCA

Downstream 100 bases:

>100_bases
AAGCAACATATTAAAGGAATAAATAATGAAAATACTGGTACCTATAAAACGAGTGGTCGATCATAACGTAAAAGTTCGTA
TCAAATCTGATGGCAGTGGT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MINNQCIQAKQKLLSPLKLGEIILNNRVVMAPMTRNRANSDGTPTQSMVQHYADRASAGLIVAEGTWPSHSGQAYSRQPG
IETKAHCQAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIKGEAVNSVAPSAIQAAGQVYTDSAGMQDFDMPLALSSVQIA
KVIAEHRQAAINAIEAGFDGVELHCTSGYLPMQFLCSGTNQRTDKYGGSSDKRASFAYECLLSMSDAIGAGRVGLRINPG
NSFNDCSDDDPAQSHISLLKQVKSLNLAYLHVMKSPLTDIDAFALAREHFNGPLILNDGFEPSSAAQAVQDEQGQAVSFA
RHFIANPDLVYRINDDLPLSKFDRKSLYTAGDAGYNDYQSHQIITTS

Sequences:

>Translated_367_residues
MINNQCIQAKQKLLSPLKLGEIILNNRVVMAPMTRNRANSDGTPTQSMVQHYADRASAGLIVAEGTWPSHSGQAYSRQPG
IETKAHCQAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIKGEAVNSVAPSAIQAAGQVYTDSAGMQDFDMPLALSSVQIA
KVIAEHRQAAINAIEAGFDGVELHCTSGYLPMQFLCSGTNQRTDKYGGSSDKRASFAYECLLSMSDAIGAGRVGLRINPG
NSFNDCSDDDPAQSHISLLKQVKSLNLAYLHVMKSPLTDIDAFALAREHFNGPLILNDGFEPSSAAQAVQDEQGQAVSFA
RHFIANPDLVYRINDDLPLSKFDRKSLYTAGDAGYNDYQSHQIITTS
>Mature_367_residues
MINNQCIQAKQKLLSPLKLGEIILNNRVVMAPMTRNRANSDGTPTQSMVQHYADRASAGLIVAEGTWPSHSGQAYSRQPG
IETKAHCQAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIKGEAVNSVAPSAIQAAGQVYTDSAGMQDFDMPLALSSVQIA
KVIAEHRQAAINAIEAGFDGVELHCTSGYLPMQFLCSGTNQRTDKYGGSSDKRASFAYECLLSMSDAIGAGRVGLRINPG
NSFNDCSDDDPAQSHISLLKQVKSLNLAYLHVMKSPLTDIDAFALAREHFNGPLILNDGFEPSSAAQAVQDEQGQAVSFA
RHFIANPDLVYRINDDLPLSKFDRKSLYTAGDAGYNDYQSHQIITTS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=37.1584699453552, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1789463, Length=221, Percent_Identity=35.7466063348416, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=357, Percent_Identity=27.4509803921569, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=353, Percent_Identity=26.628895184136, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559804, Length=251, Percent_Identity=26.6932270916335, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI72001454, Length=254, Percent_Identity=27.9527559055118, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17564188, Length=251, Percent_Identity=26.2948207171315, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=383, Percent_Identity=31.331592689295, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=31.3157894736842, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39700; Mature: 39700

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNQCIQAKQKLLSPLKLGEIILNNRVVMAPMTRNRANSDGTPTQSMVQHYADRASAGL
CCCCHHHHHHHHHHCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCE
IVAEGTWPSHSGQAYSRQPGIETKAHCQAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIK
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
GEAVNSVAPSAIQAAGQVYTDSAGMQDFDMPLALSSVQIAKVIAEHRQAAINAIEAGFDG
CCHHHHCCCHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VELHCTSGYLPMQFLCSGTNQRTDKYGGSSDKRASFAYECLLSMSDAIGAGRVGLRINPG
EEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
NSFNDCSDDDPAQSHISLLKQVKSLNLAYLHVMKSPLTDIDAFALAREHFNGPLILNDGF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCC
EPSSAAQAVQDEQGQAVSFARHFIANPDLVYRINDDLPLSKFDRKSLYTAGDAGYNDYQS
CCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHCHHHHEECCCCCCCCCCC
HQIITTS
CEEEECC
>Mature Secondary Structure
MINNQCIQAKQKLLSPLKLGEIILNNRVVMAPMTRNRANSDGTPTQSMVQHYADRASAGL
CCCCHHHHHHHHHHCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCE
IVAEGTWPSHSGQAYSRQPGIETKAHCQAWRHVTDAVHAKGGKIVLQIMHSGRIGSKHIK
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
GEAVNSVAPSAIQAAGQVYTDSAGMQDFDMPLALSSVQIAKVIAEHRQAAINAIEAGFDG
CCHHHHCCCHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VELHCTSGYLPMQFLCSGTNQRTDKYGGSSDKRASFAYECLLSMSDAIGAGRVGLRINPG
EEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
NSFNDCSDDDPAQSHISLLKQVKSLNLAYLHVMKSPLTDIDAFALAREHFNGPLILNDGF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCC
EPSSAAQAVQDEQGQAVSFARHFIANPDLVYRINDDLPLSKFDRKSLYTAGDAGYNDYQS
CCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHCHHHHEECCCCCCCCCCC
HQIITTS
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]