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The map label for this gene is tusD [H]

Identifier: 167624006

GI number: 167624006

Start: 2507223

End: 2507612

Strand: Reverse

Name: tusD [H]

Synonym: Shal_2082

Alternate gene names: 167624006

Gene position: 2507612-2507223 (Counterclockwise)

Preceding gene: 167624007

Following gene: 167624005

Centisome position: 47.97

GC content: 44.1

Gene sequence:

>390_bases
ATGAGCAAATTTATTATTCAGGTTAACTCTCCAGCTTACGGAAGTTCATCAAGTTACAGCGCGTATCGATTCGCAAAAGC
CGCGATAGACAATGGTCACATGATTGAGAAAGTATTCTTTTATCAAGATGGTGTCCTTAACACGAATAGCCTCAATAGCC
CCGCAAGTGACGAGTTTGATCTAAAGCAGGCATGGGTCGATTTACACGATAATCACGGTGTGGCCTTGATTAACTGTGTC
TCTGCCGCGCTGCGCCGTGGTGTGTTATCAGAGTCAGAAGCAAAAGAAAACCAACAGTTGCACTGGAATATGCAAGCGCC
ATTTATTATGGGTGGCTTGGGTGAGTTGGTTACTGGTATTGAGACAGCTGACAGACTTATCTGTTTTTAG

Upstream 100 bases:

>100_bases
CATCGCCCTACTGCATTTAATGGGCATCGGTAACGATGACTAATACCAATTAAATTTAATTGAGTTAAAATGGTCCTATT
ACAGGGCCATTTTTTTTACC

Downstream 100 bases:

>100_bases
GAGCATTAGTGAAGAAACTTTGTATTATCTTTAGGCAGGCTCCTCATGGCACAGCACACGGCAGAGAGGGCCTTGATCTG
TCATTGTTAAGTGCTAGTTT

Product: DsrE family protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSKFIIQVNSPAYGSSSSYSAYRFAKAAIDNGHMIEKVFFYQDGVLNTNSLNSPASDEFDLKQAWVDLHDNHGVALINCV
SAALRRGVLSESEAKENQQLHWNMQAPFIMGGLGELVTGIETADRLICF

Sequences:

>Translated_129_residues
MSKFIIQVNSPAYGSSSSYSAYRFAKAAIDNGHMIEKVFFYQDGVLNTNSLNSPASDEFDLKQAWVDLHDNHGVALINCV
SAALRRGVLSESEAKENQQLHWNMQAPFIMGGLGELVTGIETADRLICF
>Mature_128_residues
SKFIIQVNSPAYGSSSSYSAYRFAKAAIDNGHMIEKVFFYQDGVLNTNSLNSPASDEFDLKQAWVDLHDNHGVALINCVS
AALRRGVLSESEAKENQQLHWNMQAPFIMGGLGELVTGIETADRLICF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE [H]

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family [H]

Homologues:

Organism=Escherichia coli, GI1789743, Length=127, Percent_Identity=50.3937007874016, Blast_Score=129, Evalue=6e-32,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787
- InterPro:   IPR017463 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: NA

Molecular weight: Translated: 14231; Mature: 14100

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFIIQVNSPAYGSSSSYSAYRFAKAAIDNGHMIEKVFFYQDGVLNTNSLNSPASDEFD
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCH
LKQAWVDLHDNHGVALINCVSAALRRGVLSESEAKENQQLHWNMQAPFIMGGLGELVTGI
HHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHH
ETADRLICF
HHHHHEECC
>Mature Secondary Structure 
SKFIIQVNSPAYGSSSSYSAYRFAKAAIDNGHMIEKVFFYQDGVLNTNSLNSPASDEFD
CEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCH
LKQAWVDLHDNHGVALINCVSAALRRGVLSESEAKENQQLHWNMQAPFIMGGLGELVTGI
HHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHH
ETADRLICF
HHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA