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The map label for this gene is gap [H]

Identifier: 167624002

GI number: 167624002

Start: 2503325

End: 2504761

Strand: Direct

Name: gap [H]

Synonym: Shal_2078

Alternate gene names: 167624002

Gene position: 2503325-2504761 (Clockwise)

Preceding gene: 167623999

Following gene: 167624009

Centisome position: 47.89

GC content: 43.22

Gene sequence:

>1437_bases
ATGAGCGCTGATAAACACCTACAAAGTTGGCAAGAACGTTTCGAAATGGCTGAGGCAATGCAGCCATTACTTGGTAAGTT
ATACCGTAACCAAGGTGTAGAAGTGGTCGTCTATGGCAAACCTCTACTGAATGCTTCTACAATCGAAATCATTAAATCGC
ACCGTTTAGTGCGTCGCCATGTTGGTGAAAAACTAAGACTACGTGAAAGTTTTCCATTTGTTGAGGCTTTAAGCAAGTTA
GCGGTAAAACAGTGTAAAGTTGATATCGGTAAATTAGCGGTTAATTACTGGCGCGAATATCAAGATGCAAGTCAGGTTGA
GGCTTATATGAGCCGCGAACTTGCAGATGCAATCACCCATCAAGATGAGCAACAACCAAGAGATGTCGTTCTTTATGGTT
TTGGTCGTATCGGTCGTCTACTCGCTCGTTTGCTTATTGAAAGAACGGGTGTAAGCAACAAGCTTAGACTACGTGCTATC
GTACTTCGTGGTGGCCGTACTGGCGATTTAGAAAAGCGTGCAAGCTTATTGCGTCGAGATTCGGTTCACGGTCCATTTAA
TGGCTCTGTTGAAGTTGATGAAGAGAACAATGCAATTATCGCAAACGGTACCTACATTCAGGTTATCTACGCGAACTCTC
CAGATGAAGTTGATTACACTAAATTTGGTATTTCAAATGCACTTGTGGTTGATAACACTGGTATTTGGAAAGATGAAGCG
GGCCTTGGCTTACACCTTAAGTCACCTGGTGCGAGCAAAGTATTGCTCACAGCCCCTGCTAAGGGTGCTATTAAGAACAT
CGTTTATGGTGTGAACGAGTCAGATATTCTTGATGAAGATACCATTGTATCGGCTGCAAGCTGTACGACTAACGCTATTA
CGCCTGTGCTTAAAGCGGTAAATGATAAGTACGGTATCGTAAATGGTCACGTTGAAACGATTCACTCGTATACCAATGAC
CAAAACCTTATCGATAACTACCACAGTGCAGATCGCCGTGGACGTAGTGCACCACTAAACATGGTTATTACTGAAACCGG
TGCGGCTAAAGCGGTTTCAAAAGCGCTACCAGTACTTGAAGGTAAGTTAACAGGTAATGCTATTCGTGTTCCAACACCCA
ATGTGTCTATGGCGATTATTAGCCTTAACCTAGAGGGTGAAACGAACAAGCACGACATGAACGAGTACCTAAAAGACATG
GCATTGCAGTCTGAACTGCAAAACCAAATCGACTACACTGATTCTACAGAAATCGTATCAAGTGATCTTGTGGGTTCTCG
TTATGCGGGTGTAATTGATTCGCAAGCAACGATTGCTGAAGGTAACCGTACTATTCTTTATGTATGGTATGACAACGAAT
TTGGCTATAGCTGTCAGGTTGTCGGCGTAATGCAAAAAATGCTTGGCCTAAACTACCAGTCATTGCCCCATGGCTAA

Upstream 100 bases:

>100_bases
GGAAAGACACCCTATATAACGTTAGATTATTGGTCTTTTCTTTTTGTTACTCAAACATTTCAGACAAAAAAGTCAGAAAA
GTGAGATAAAGGATCTGTTA

Downstream 100 bases:

>100_bases
TTCGTTATAGAGTTAAGCTTAGCGAATAAAACGCCTCTATTTTTAGAGGCGTTTTTGCTTTTTAAACTTCCGACTTTCAA
AATATGCAATTTCATCCAAA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRHVGEKLRLRESFPFVEALSKL
AVKQCKVDIGKLAVNYWREYQDASQVEAYMSRELADAITHQDEQQPRDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAI
VLRGGRTGDLEKRASLLRRDSVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
GLGLHLKSPGASKVLLTAPAKGAIKNIVYGVNESDILDEDTIVSAASCTTNAITPVLKAVNDKYGIVNGHVETIHSYTND
QNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLEGKLTGNAIRVPTPNVSMAIISLNLEGETNKHDMNEYLKDM
ALQSELQNQIDYTDSTEIVSSDLVGSRYAGVIDSQATIAEGNRTILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG

Sequences:

>Translated_478_residues
MSADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRHVGEKLRLRESFPFVEALSKL
AVKQCKVDIGKLAVNYWREYQDASQVEAYMSRELADAITHQDEQQPRDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAI
VLRGGRTGDLEKRASLLRRDSVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
GLGLHLKSPGASKVLLTAPAKGAIKNIVYGVNESDILDEDTIVSAASCTTNAITPVLKAVNDKYGIVNGHVETIHSYTND
QNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLEGKLTGNAIRVPTPNVSMAIISLNLEGETNKHDMNEYLKDM
ALQSELQNQIDYTDSTEIVSSDLVGSRYAGVIDSQATIAEGNRTILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG
>Mature_477_residues
SADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRHVGEKLRLRESFPFVEALSKLA
VKQCKVDIGKLAVNYWREYQDASQVEAYMSRELADAITHQDEQQPRDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAIV
LRGGRTGDLEKRASLLRRDSVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEAG
LGLHLKSPGASKVLLTAPAKGAIKNIVYGVNESDILDEDTIVSAASCTTNAITPVLKAVNDKYGIVNGHVETIHSYTNDQ
NLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLEGKLTGNAIRVPTPNVSMAIISLNLEGETNKHDMNEYLKDMA
LQSELQNQIDYTDSTEIVSSDLVGSRYAGVIDSQATIAEGNRTILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=339, Percent_Identity=36.8731563421829, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI7669492, Length=337, Percent_Identity=36.7952522255193, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1788079, Length=338, Percent_Identity=36.3905325443787, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789295, Length=347, Percent_Identity=31.9884726224784, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17534677, Length=338, Percent_Identity=35.5029585798817, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17534679, Length=338, Percent_Identity=35.207100591716, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17568413, Length=340, Percent_Identity=35.8823529411765, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32566163, Length=340, Percent_Identity=35.8823529411765, Blast_Score=171, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6322409, Length=339, Percent_Identity=36.283185840708, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322468, Length=339, Percent_Identity=36.283185840708, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321631, Length=340, Percent_Identity=36.7647058823529, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=38.368580060423, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI85725000, Length=337, Percent_Identity=36.2017804154303, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI22023983, Length=337, Percent_Identity=36.2017804154303, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17933600, Length=337, Percent_Identity=35.9050445103858, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI18110149, Length=337, Percent_Identity=35.9050445103858, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 52837; Mature: 52705

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VGEKLRLRESFPFVEALSKLAVKQCKVDIGKLAVNYWREYQDASQVEAYMSRELADAITH
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
QDEQQPRDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAIVLRGGRTGDLEKRASLLRRD
CCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHC
SVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
CCCCCCCCCEEEECCCCEEEECCCEEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCCC
GLGLHLKSPGASKVLLTAPAKGAIKNIVYGVNESDILDEDTIVSAASCTTNAITPVLKAV
CCEEEECCCCCCEEEEECCCCHHHHHHEECCCCCCCCCCCHHEEHHHCCHHHHHHHHHHH
NDKYGIVNGHVETIHSYTNDQNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLE
CCCCCEECCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHC
GKLTGNAIRVPTPNVSMAIISLNLEGETNKHDMNEYLKDMALQSELQNQIDYTDSTEIVS
CEECCCEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SDLVGSRYAGVIDSQATIAEGNRTILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG
HHCCCCHHCCCCCCCCEEECCCEEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VGEKLRLRESFPFVEALSKLAVKQCKVDIGKLAVNYWREYQDASQVEAYMSRELADAITH
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
QDEQQPRDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAIVLRGGRTGDLEKRASLLRRD
CCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHC
SVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
CCCCCCCCCEEEECCCCEEEECCCEEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCCC
GLGLHLKSPGASKVLLTAPAKGAIKNIVYGVNESDILDEDTIVSAASCTTNAITPVLKAV
CCEEEECCCCCCEEEEECCCCHHHHHHEECCCCCCCCCCCHHEEHHHCCHHHHHHHHHHH
NDKYGIVNGHVETIHSYTNDQNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLE
CCCCCEECCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHC
GKLTGNAIRVPTPNVSMAIISLNLEGETNKHDMNEYLKDMALQSELQNQIDYTDSTEIVS
CEECCCEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SDLVGSRYAGVIDSQATIAEGNRTILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG
HHCCCCHHCCCCCCCCEEECCCEEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8508805; 10360571; 2401296; 7877172 [H]