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The map label for this gene is dacB [H]

Identifier: 167623981

GI number: 167623981

Start: 2478080

End: 2479510

Strand: Reverse

Name: dacB [H]

Synonym: Shal_2055

Alternate gene names: 167623981

Gene position: 2479510-2478080 (Counterclockwise)

Preceding gene: 167623982

Following gene: 167623979

Centisome position: 47.44

GC content: 45.14

Gene sequence:

>1431_bases
TTGCTTAAACGCTTTGTAGCTGCCACAACGCTTTGCCTTATTGCCACCTCGGCTGTAGCCGACGATTACCTCGCTAATAT
GGTAAAGGCGATAGAACCTAAAACATCTCAAACCGCCATCGTTGTGAGCCCCCTCAATTTAAACGATGAGGGACACGTTA
GCTCGCCAGCATACCGTAAAGATGCGCAACGACTATTTGTACCGGCCAGCACGATGAAGCTGCTTACAGCCGTTGCGGCA
ACGACAGCGCTAGGTAAAGATTTTCGATTTAAAACCCAAATTGACTCATTTGTTGGAATTAAGAACCGACGAATTGAGGG
CGATCTATTTATACGCTTTGATGGGGATCCCACTCTCACTGGTCGCGACTTAAGACAGTTATTCAAGCAGCTAAAGAAGC
ATGGGTTAGATCATATTGATGGCAACCTTTATTTAATCGGTGATCAGCAAGAAACACTGCAAGCTCCCGGCTGGGTATGG
GACGATTTAGGGATCTGCTACGCGGCACCAGTATCTCGCTATATCGTTAATCAAAACTGTGTTAAAGCGAAGCTCTCACC
AACCCTCGCCGACAATCAGGGTAAACTGACATTTAGCCAATTTGAACCCGTATCAATCGCCAGCACCGCAGTTTTTGATA
AAGTCGGAGACACACCTTTTTGCGAGTTAAGCCTACAACGCCAAGCTAATAATGAGTTTATTCTATCGGGCTGTCATGCT
GGGCAGAAGCCATTAAATCTAGCTATTGCCATATCAGATCCGGCGCTCTATGCCCAAAAGACGGTAGCAAATACCTTAAC
CAGCATTGGGATCAACATTAAAGGTGATGTGCTATTAACCAGTAAGCGTCCTGCCAATACTATATTGCTCGCTGAGCACC
AATCTAAGCCGCTTTCTGAGCTGACTGACATCATGTTATTAAAATCAGATAACTTAATTGCTGACAGCCTACTAAAGCGC
TTAGGACAATATATTTATGGCGTTCCGGGCACATTTATTAACGGTAGCGCTGCAATGAAACAGATCTTAACCGAACAAGG
CGTCGATCTTTCCAGTGCAAATATTGTCGATGGCTCGGGGCTTTCTCGTTATAACCTGCTTAGCGCAGATCAGTTAATCC
AAGTGCTATTATTGCTCAAGCAGAAAGAACAACTCAACTTTTTAATTGATCAACTTCCGATAGCCGGCAAATCTGGCACC
TTAAAATATCGAAACGGTTACACTGCATCCCCCCTCAAAGGCAGCGTATTGGCCAAAACGGGTTCGATGATGGGAGTCAC
CAACTTAGCAGGGTTTATTAAAAAGGATGGACAACTTTCCCAGGCCTTTGTGATCTTAGAAAATGGTCATAGCCCATCGG
TGAAAAAGAGCGAGCTAGCACCTTTTAATGTTCTGTTTTTACAGTCTATGGTGCAAAGGGCGCGCCCCTGA

Upstream 100 bases:

>100_bases
TGTTTGTGACACTAAGGCTTTATCGTTTGATGGAAAAGGATGAACCTAATGGTCAAGACTAGGCTAATGTTGCTCATTTT
AATAATAGAAGGGATCTCTT

Downstream 100 bases:

>100_bases
CTTGGACGCTTGTAGACTTTAACTTAAAGGTGCAGCAAAGCCAGAAGTTATCACCAACTAAGCCCGTTGCAACTAGCGTG
TTTAAGACTGAAACTAAAAG

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MLKRFVAATTLCLIATSAVADDYLANMVKAIEPKTSQTAIVVSPLNLNDEGHVSSPAYRKDAQRLFVPASTMKLLTAVAA
TTALGKDFRFKTQIDSFVGIKNRRIEGDLFIRFDGDPTLTGRDLRQLFKQLKKHGLDHIDGNLYLIGDQQETLQAPGWVW
DDLGICYAAPVSRYIVNQNCVKAKLSPTLADNQGKLTFSQFEPVSIASTAVFDKVGDTPFCELSLQRQANNEFILSGCHA
GQKPLNLAIAISDPALYAQKTVANTLTSIGINIKGDVLLTSKRPANTILLAEHQSKPLSELTDIMLLKSDNLIADSLLKR
LGQYIYGVPGTFINGSAAMKQILTEQGVDLSSANIVDGSGLSRYNLLSADQLIQVLLLLKQKEQLNFLIDQLPIAGKSGT
LKYRNGYTASPLKGSVLAKTGSMMGVTNLAGFIKKDGQLSQAFVILENGHSPSVKKSELAPFNVLFLQSMVQRARP

Sequences:

>Translated_476_residues
MLKRFVAATTLCLIATSAVADDYLANMVKAIEPKTSQTAIVVSPLNLNDEGHVSSPAYRKDAQRLFVPASTMKLLTAVAA
TTALGKDFRFKTQIDSFVGIKNRRIEGDLFIRFDGDPTLTGRDLRQLFKQLKKHGLDHIDGNLYLIGDQQETLQAPGWVW
DDLGICYAAPVSRYIVNQNCVKAKLSPTLADNQGKLTFSQFEPVSIASTAVFDKVGDTPFCELSLQRQANNEFILSGCHA
GQKPLNLAIAISDPALYAQKTVANTLTSIGINIKGDVLLTSKRPANTILLAEHQSKPLSELTDIMLLKSDNLIADSLLKR
LGQYIYGVPGTFINGSAAMKQILTEQGVDLSSANIVDGSGLSRYNLLSADQLIQVLLLLKQKEQLNFLIDQLPIAGKSGT
LKYRNGYTASPLKGSVLAKTGSMMGVTNLAGFIKKDGQLSQAFVILENGHSPSVKKSELAPFNVLFLQSMVQRARP
>Mature_476_residues
MLKRFVAATTLCLIATSAVADDYLANMVKAIEPKTSQTAIVVSPLNLNDEGHVSSPAYRKDAQRLFVPASTMKLLTAVAA
TTALGKDFRFKTQIDSFVGIKNRRIEGDLFIRFDGDPTLTGRDLRQLFKQLKKHGLDHIDGNLYLIGDQQETLQAPGWVW
DDLGICYAAPVSRYIVNQNCVKAKLSPTLADNQGKLTFSQFEPVSIASTAVFDKVGDTPFCELSLQRQANNEFILSGCHA
GQKPLNLAIAISDPALYAQKTVANTLTSIGINIKGDVLLTSKRPANTILLAEHQSKPLSELTDIMLLKSDNLIADSLLKR
LGQYIYGVPGTFINGSAAMKQILTEQGVDLSSANIVDGSGLSRYNLLSADQLIQVLLLLKQKEQLNFLIDQLPIAGKSGT
LKYRNGYTASPLKGSVLAKTGSMMGVTNLAGFIKKDGQLSQAFVILENGHSPSVKKSELAPFNVLFLQSMVQRARP

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=481, Percent_Identity=36.1746361746362, Blast_Score=271, Evalue=9e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51729; Mature: 51729

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFVAATTLCLIATSAVADDYLANMVKAIEPKTSQTAIVVSPLNLNDEGHVSSPAYRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHH
DAQRLFVPASTMKLLTAVAATTALGKDFRFKTQIDSFVGIKNRRIEGDLFIRFDGDPTLT
CCCEEEECHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEECCEEEEEECCCCCCC
GRDLRQLFKQLKKHGLDHIDGNLYLIGDQQETLQAPGWVWDDLGICYAAPVSRYIVNQNC
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCCEECCCCHHHHHHHHHHHHCCCH
VKAKLSPTLADNQGKLTFSQFEPVSIASTAVFDKVGDTPFCELSLQRQANNEFILSGCHA
HEEECCCCEECCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCC
GQKPLNLAIAISDPALYAQKTVANTLTSIGINIKGDVLLTSKRPANTILLAEHQSKPLSE
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCEEEEEECCCCCHHH
LTDIMLLKSDNLIADSLLKRLGQYIYGVPGTFINGSAAMKQILTEQGVDLSSANIVDGSG
HHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCCCCEECCCC
LSRYNLLSADQLIQVLLLLKQKEQLNFLIDQLPIAGKSGTLKYRNGYTASPLKGSVLAKT
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEECC
GSMMGVTNLAGFIKKDGQLSQAFVILENGHSPSVKKSELAPFNVLFLQSMVQRARP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKRFVAATTLCLIATSAVADDYLANMVKAIEPKTSQTAIVVSPLNLNDEGHVSSPAYRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHH
DAQRLFVPASTMKLLTAVAATTALGKDFRFKTQIDSFVGIKNRRIEGDLFIRFDGDPTLT
CCCEEEECHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEECCEEEEEECCCCCCC
GRDLRQLFKQLKKHGLDHIDGNLYLIGDQQETLQAPGWVWDDLGICYAAPVSRYIVNQNC
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCCEECCCCHHHHHHHHHHHHCCCH
VKAKLSPTLADNQGKLTFSQFEPVSIASTAVFDKVGDTPFCELSLQRQANNEFILSGCHA
HEEECCCCEECCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCC
GQKPLNLAIAISDPALYAQKTVANTLTSIGINIKGDVLLTSKRPANTILLAEHQSKPLSE
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCEEEEEECCCCCHHH
LTDIMLLKSDNLIADSLLKRLGQYIYGVPGTFINGSAAMKQILTEQGVDLSSANIVDGSG
HHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCCCCEECCCC
LSRYNLLSADQLIQVLLLLKQKEQLNFLIDQLPIAGKSGTLKYRNGYTASPLKGSVLAKT
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEECC
GSMMGVTNLAGFIKKDGQLSQAFVILENGHSPSVKKSELAPFNVLFLQSMVQRARP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]