Click here to switch to the map view.

The map label for this gene is aprN [H]

Identifier: 167623908

GI number: 167623908

Start: 2382212

End: 2384032

Strand: Reverse

Name: aprN [H]

Synonym: Shal_1979

Alternate gene names: 167623908

Gene position: 2384032-2382212 (Counterclockwise)

Preceding gene: 167623909

Following gene: 167623906

Centisome position: 45.61

GC content: 47.28

Gene sequence:

>1821_bases
ATGAGAAAAACAACTAAAACTCTTATCGCCTTGAGCATAGGGTTATCATTTGCAGCTCAGGCCGCGGATCAACGCTATAT
CATCCAAGTCGATGAAGGTCACAAGGGATTAGTCAAAGCCTTAGCAAAAAAAGTGGGCGGCGAGCTTAAAGTTGACGGCA
ACGGATTTATTGCAGCAACATTTGCAGGAAAAGATCTTAGCACTGTTAAAGGACTCCTCAATAACCCGCATGTGACACTT
GTAGAAGAAGATCAACGCCGCTTACCACTCGCGTTATATAACGATGACATTGGCGATCCTTCCGTCACACAGCTAACACC
TTATGCCGTTTACCAGTCGCAAGCTAACCAGCTGACATTTAATCCCAGTGCGGGTATGAAAGTTTGTGTGATTGACTCGG
GACTCGATCGCTCTAATCCCGATTTTGAATGGTCATCAATTACCGGTGACAATGACATTGGCACCGGAAACTGGGATGAA
AATGGTGGACCTCATGGTACCCATGTAGCCGGAACCATTGGCGCAGCAGACAATGACATTGGTGTGGTTGGCATGGCACC
AGGTGTCGAAATGCATATTATCAAGGTCTTCAATGCACAGGGCTGGGGCTACTCATCAGACTTGGCACACGCAGCTAATC
TTTGTAACGCAGCCGGTGCAAATATCATCAGCATGAGTCTAGGTGGTGGCGGTGCCAATAGCACCGAAGAAAATGCCTTT
AAAACCTTTAGCTCTTCAGGTGGCCTTGTAGTGGCCGCAGCAGGTAATGACGGTAATAATGTACGTTCATACCCAGCAGG
CTACACCTCGGTGATGATGGTGGGTGCAAATGATGCAGACAATAATATTGCTGCATTTTCACAATTCCCAGCCTGCTCAA
CCTTTTCTACCGGGCGTGGCAAGAAAAATAAAACTGAGATTATCGATGGTAGTTGCGTTGAGATCACCGCTGGTGGTGTC
AGCACGCTATCAACCTATCCTCAAGACATGGCGACCAATTCCAATCTTGATGCGGACGGGCAAACCATCGCCTCTTCGGC
AATGGAAAACTCCGGGCTAGTAACAGCCGAACCCTACTTTATGGGGATAGCCGAATCAATTGATGCGAGCGCCAATGGCA
AAATTTGTCTGATTGATCGAGGTACTATCTCTTTTCACGATAAGGTTAAAAACTGTGAAGCCTCAGGTGGTTTAGGTGCT
GTTATCGTAAACAATGTCGATGGCATGTTATATGGCACTCTTGGTGATGCCAATATCACCTCTATTCCAGCGCTAGGTAC
CACACTCGCCGATCGCGGCGTCTTACATGCTGCAACACAAATGACGGTGAAAATTGCCGCCAGCGACTACGGATTCATGA
GCGGAACCTCAATGGCAACACCAGCTATCTCTGGATTGGCTGCGCTTTTATGGTCAAACCATAGTGGCTGTACAGGTAAT
GATATTCGTGAAGCACTAAAAGCCTCAGCATACGATGCTGGTGAGTCGGGCCGCGATAATTACTTTGGTTACGGTATTGC
CAAAGCCGCCGATGCCAGTGCTTACCTCGACAGTATGGGTTGTAATACGACCGCGCCAGTAGAACAGATTGAGCTTAGCG
CTACAACATCATTCTCGAAAGGCAAAGACAGGGTAAGCTTAACATGGTCTGGTGCCAGCACCGGACGCGTCGATGTTTAT
CGTAATGGTCTTATTCAAACCACAACCAGCAATGATGGCAGCCACATAGACACATTAAGAAATGCTTATGGCATGTACAC
TTATAGTGTTTGTGATGCGGGTACAAATCACTGCTCTAGCAATGTGAGTGTCACGTTTTAA

Upstream 100 bases:

>100_bases
ATGGCAGTCGCTTTATTACAAATGCCCAGTTTTTTGCAGCTTATCAGCAATATTCAAATAGACATATTTAATAAGAATCA
TAAATAAATGGAGTGTATTA

Downstream 100 bases:

>100_bases
TTTATTTGGTTGAACTTGCTAGCGACAATCAATCAACATAAATAGCCTAAAAAGCCGCATTAGCGGCTTTTTTATTGCAA
GTTAAAAAAACAAAAACAGG

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MRKTTKTLIALSIGLSFAAQAADQRYIIQVDEGHKGLVKALAKKVGGELKVDGNGFIAATFAGKDLSTVKGLLNNPHVTL
VEEDQRRLPLALYNDDIGDPSVTQLTPYAVYQSQANQLTFNPSAGMKVCVIDSGLDRSNPDFEWSSITGDNDIGTGNWDE
NGGPHGTHVAGTIGAADNDIGVVGMAPGVEMHIIKVFNAQGWGYSSDLAHAANLCNAAGANIISMSLGGGGANSTEENAF
KTFSSSGGLVVAAAGNDGNNVRSYPAGYTSVMMVGANDADNNIAAFSQFPACSTFSTGRGKKNKTEIIDGSCVEITAGGV
STLSTYPQDMATNSNLDADGQTIASSAMENSGLVTAEPYFMGIAESIDASANGKICLIDRGTISFHDKVKNCEASGGLGA
VIVNNVDGMLYGTLGDANITSIPALGTTLADRGVLHAATQMTVKIAASDYGFMSGTSMATPAISGLAALLWSNHSGCTGN
DIREALKASAYDAGESGRDNYFGYGIAKAADASAYLDSMGCNTTAPVEQIELSATTSFSKGKDRVSLTWSGASTGRVDVY
RNGLIQTTTSNDGSHIDTLRNAYGMYTYSVCDAGTNHCSSNVSVTF

Sequences:

>Translated_606_residues
MRKTTKTLIALSIGLSFAAQAADQRYIIQVDEGHKGLVKALAKKVGGELKVDGNGFIAATFAGKDLSTVKGLLNNPHVTL
VEEDQRRLPLALYNDDIGDPSVTQLTPYAVYQSQANQLTFNPSAGMKVCVIDSGLDRSNPDFEWSSITGDNDIGTGNWDE
NGGPHGTHVAGTIGAADNDIGVVGMAPGVEMHIIKVFNAQGWGYSSDLAHAANLCNAAGANIISMSLGGGGANSTEENAF
KTFSSSGGLVVAAAGNDGNNVRSYPAGYTSVMMVGANDADNNIAAFSQFPACSTFSTGRGKKNKTEIIDGSCVEITAGGV
STLSTYPQDMATNSNLDADGQTIASSAMENSGLVTAEPYFMGIAESIDASANGKICLIDRGTISFHDKVKNCEASGGLGA
VIVNNVDGMLYGTLGDANITSIPALGTTLADRGVLHAATQMTVKIAASDYGFMSGTSMATPAISGLAALLWSNHSGCTGN
DIREALKASAYDAGESGRDNYFGYGIAKAADASAYLDSMGCNTTAPVEQIELSATTSFSKGKDRVSLTWSGASTGRVDVY
RNGLIQTTTSNDGSHIDTLRNAYGMYTYSVCDAGTNHCSSNVSVTF
>Mature_606_residues
MRKTTKTLIALSIGLSFAAQAADQRYIIQVDEGHKGLVKALAKKVGGELKVDGNGFIAATFAGKDLSTVKGLLNNPHVTL
VEEDQRRLPLALYNDDIGDPSVTQLTPYAVYQSQANQLTFNPSAGMKVCVIDSGLDRSNPDFEWSSITGDNDIGTGNWDE
NGGPHGTHVAGTIGAADNDIGVVGMAPGVEMHIIKVFNAQGWGYSSDLAHAANLCNAAGANIISMSLGGGGANSTEENAF
KTFSSSGGLVVAAAGNDGNNVRSYPAGYTSVMMVGANDADNNIAAFSQFPACSTFSTGRGKKNKTEIIDGSCVEITAGGV
STLSTYPQDMATNSNLDADGQTIASSAMENSGLVTAEPYFMGIAESIDASANGKICLIDRGTISFHDKVKNCEASGGLGA
VIVNNVDGMLYGTLGDANITSIPALGTTLADRGVLHAATQMTVKIAASDYGFMSGTSMATPAISGLAALLWSNHSGCTGN
DIREALKASAYDAGESGRDNYFGYGIAKAADASAYLDSMGCNTTAPVEQIELSATTSFSKGKDRVSLTWSGASTGRVDVY
RNGLIQTTTSNDGSHIDTLRNAYGMYTYSVCDAGTNHCSSNVSVTF

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 62737; Mature: 62737

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTTKTLIALSIGLSFAAQAADQRYIIQVDEGHKGLVKALAKKVGGELKVDGNGFIAAT
CCCHHHEEEEEECCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCEEEEE
FAGKDLSTVKGLLNNPHVTLVEEDQRRLPLALYNDDIGDPSVTQLTPYAVYQSQANQLTF
ECCCCHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCHHEECCHHEECCCCCEEEE
NPSAGMKVCVIDSGLDRSNPDFEWSSITGDNDIGTGNWDENGGPHGTHVAGTIGAADNDI
CCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCE
GVVGMAPGVEMHIIKVFNAQGWGYSSDLAHAANLCNAAGANIISMSLGGGGANSTEENAF
EEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
KTFSSSGGLVVAAAGNDGNNVRSYPAGYTSVMMVGANDADNNIAAFSQFPACSTFSTGRG
HHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHCCCCCCCCCCCCC
KKNKTEIIDGSCVEITAGGVSTLSTYPQDMATNSNLDADGQTIASSAMENSGLVTAEPYF
CCCCCEEECCCEEEEECCCCHHHHCCCHHHCCCCCCCCCCHHHHHHHHCCCCCEEECHHH
MGIAESIDASANGKICLIDRGTISFHDKVKNCEASGGLGAVIVNNVDGMLYGTLGDANIT
EEHHHHCCCCCCCCEEEEECCCEEEHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCC
SIPALGTTLADRGVLHAATQMTVKIAASDYGFMSGTSMATPAISGLAALLWSNHSGCTGN
CCCCCCHHHHHCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
DIREALKASAYDAGESGRDNYFGYGIAKAADASAYLDSMGCNTTAPVEQIELSATTSFSK
HHHHHHHHHCCCCCCCCCCCCCEEEEHHHCCHHHHHHHCCCCCCCCHHHEEEEEECCCCC
GKDRVSLTWSGASTGRVDVYRNGLIQTTTSNDGSHIDTLRNAYGMYTYSVCDAGTNHCSS
CCCEEEEEECCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHCEEEEEEECCCCCCCCC
NVSVTF
CCEEEC
>Mature Secondary Structure
MRKTTKTLIALSIGLSFAAQAADQRYIIQVDEGHKGLVKALAKKVGGELKVDGNGFIAAT
CCCHHHEEEEEECCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCEEEEE
FAGKDLSTVKGLLNNPHVTLVEEDQRRLPLALYNDDIGDPSVTQLTPYAVYQSQANQLTF
ECCCCHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCHHEECCHHEECCCCCEEEE
NPSAGMKVCVIDSGLDRSNPDFEWSSITGDNDIGTGNWDENGGPHGTHVAGTIGAADNDI
CCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCE
GVVGMAPGVEMHIIKVFNAQGWGYSSDLAHAANLCNAAGANIISMSLGGGGANSTEENAF
EEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
KTFSSSGGLVVAAAGNDGNNVRSYPAGYTSVMMVGANDADNNIAAFSQFPACSTFSTGRG
HHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHCCCCCCCCCCCCC
KKNKTEIIDGSCVEITAGGVSTLSTYPQDMATNSNLDADGQTIASSAMENSGLVTAEPYF
CCCCCEEECCCEEEEECCCCHHHHCCCHHHCCCCCCCCCCHHHHHHHHCCCCCEEECHHH
MGIAESIDASANGKICLIDRGTISFHDKVKNCEASGGLGAVIVNNVDGMLYGTLGDANIT
EEHHHHCCCCCCCCEEEEECCCEEEHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCC
SIPALGTTLADRGVLHAATQMTVKIAASDYGFMSGTSMATPAISGLAALLWSNHSGCTGN
CCCCCCHHHHHCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
DIREALKASAYDAGESGRDNYFGYGIAKAADASAYLDSMGCNTTAPVEQIELSATTSFSK
HHHHHHHHHCCCCCCCCCCCCCEEEEHHHCCHHHHHHHCCCCCCCCHHHEEEEEECCCCC
GKDRVSLTWSGASTGRVDVYRNGLIQTTTSNDGSHIDTLRNAYGMYTYSVCDAGTNHCSS
CCCEEEEEECCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHCEEEEEEECCCCCCCCC
NVSVTF
CCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]