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The map label for this gene is pleD [H]

Identifier: 167623887

GI number: 167623887

Start: 2352073

End: 2353125

Strand: Direct

Name: pleD [H]

Synonym: Shal_1957

Alternate gene names: 167623887

Gene position: 2352073-2353125 (Clockwise)

Preceding gene: 167623886

Following gene: 167623889

Centisome position: 45.0

GC content: 40.46

Gene sequence:

>1053_bases
ATGGATATGGCAAAGCATTTATCAGAAACAGAACTGTCGGCTAAGATATTACGCCATGCAGTGCCTAAAATGTCTGAACT
CAAGGTTCCAGTCACTCCTGAGAATTATGCCGTTTGGTATGAATACTATAAAGGGATTAATCTTGATTTAAAGCGTGCGA
TCGATGGATTACTGAGCAATAACGTCGCCTTTACACCAGATGTCAGCAGTAGTCTTTATAAAAACTATATCTTGATTAAC
TCACCCGAAATTATTGAGAATGTCCAATTAGAAACACAAGTACTGATAAATAGTTTACTGGCCAAAATTGGCTCTTTGAG
CAAGGACACATCACGATTTTCAGATTCATTAACTGAATTTGATAACGCATTGAAACAGGATCCCGACCCCGTTGTCATGC
AGAAACTGGTTAAAGGGATCACCAGTGAGCTTGATGAAATCATTGCCGTTAATGGTGAAATGGATAAAAGCCTAACGAGC
ATGAATGAAGAGGTTATTGCCCTTAAATCAGAAATGGTAGAGCTTCGCTCCGTTGCTATGACCGATCAGTTAACCTCAGT
GAATAATCGCCGTGCATTCGATAAAGAAGTGATTAGCCATATCGATAGTTTTAATCATTCAAATTTTGACAGTAGTTTGT
TAGTCGTCGATATTGACCACTTTAAGCGGTTTAATGACATACATGGCCATTTAGTCGGTGACAAAGTACTGGCTTACGTT
GCCCAGGCATTAAAAAATGGCGTTAAAGGCGATGATTTCGTTGCCCGTTATGGCGGTGAAGAGTTTGTTATCCTGTTGCC
TAATACCACACAGGAAGAAGCGAATATCGTAGCCGAGAATTTAAGGCAAAAGATCGCGGAGCGTAATTTGACCATAGGTA
AAGACAAAAAGCTGCCGCTTGGGAATATTACCGTGTCAGTAGGAGTTGCATCGCTAAAAGCAGCAGATGATAAAGATAGC
TATTTTATTCGTGCCGATGAAGCCTTATACCGTGCTAAGTCTTCGGGCCGAAACTGTGTCGTAAGTGAAAGAATTACAGA
GACAGCTATTTAG

Upstream 100 bases:

>100_bases
GAAAATAAAATCTAATTTATCTCTAAAAAGGGATGGAAACATCTTCATTTTTGTTTAAAGTATTGATAAGTAAAGCTATC
AAGATGGAGTGACTTTTAAC

Downstream 100 bases:

>100_bases
TATGGGCGTGGTTTATACCAGGATTTAGAACAGGATTCGAAATTGGGCATTATTAAAAACAGTGTTAATTGAGAAAGGCC
GTTAAGATAAATCTTAACGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDMAKHLSETELSAKILRHAVPKMSELKVPVTPENYAVWYEYYKGINLDLKRAIDGLLSNNVAFTPDVSSSLYKNYILIN
SPEIIENVQLETQVLINSLLAKIGSLSKDTSRFSDSLTEFDNALKQDPDPVVMQKLVKGITSELDEIIAVNGEMDKSLTS
MNEEVIALKSEMVELRSVAMTDQLTSVNNRRAFDKEVISHIDSFNHSNFDSSLLVVDIDHFKRFNDIHGHLVGDKVLAYV
AQALKNGVKGDDFVARYGGEEFVILLPNTTQEEANIVAENLRQKIAERNLTIGKDKKLPLGNITVSVGVASLKAADDKDS
YFIRADEALYRAKSSGRNCVVSERITETAI

Sequences:

>Translated_350_residues
MDMAKHLSETELSAKILRHAVPKMSELKVPVTPENYAVWYEYYKGINLDLKRAIDGLLSNNVAFTPDVSSSLYKNYILIN
SPEIIENVQLETQVLINSLLAKIGSLSKDTSRFSDSLTEFDNALKQDPDPVVMQKLVKGITSELDEIIAVNGEMDKSLTS
MNEEVIALKSEMVELRSVAMTDQLTSVNNRRAFDKEVISHIDSFNHSNFDSSLLVVDIDHFKRFNDIHGHLVGDKVLAYV
AQALKNGVKGDDFVARYGGEEFVILLPNTTQEEANIVAENLRQKIAERNLTIGKDKKLPLGNITVSVGVASLKAADDKDS
YFIRADEALYRAKSSGRNCVVSERITETAI
>Mature_350_residues
MDMAKHLSETELSAKILRHAVPKMSELKVPVTPENYAVWYEYYKGINLDLKRAIDGLLSNNVAFTPDVSSSLYKNYILIN
SPEIIENVQLETQVLINSLLAKIGSLSKDTSRFSDSLTEFDNALKQDPDPVVMQKLVKGITSELDEIIAVNGEMDKSLTS
MNEEVIALKSEMVELRSVAMTDQLTSVNNRRAFDKEVISHIDSFNHSNFDSSLLVVDIDHFKRFNDIHGHLVGDKVLAYV
AQALKNGVKGDDFVARYGGEEFVILLPNTTQEEANIVAENLRQKIAERNLTIGKDKKLPLGNITVSVGVASLKAADDKDS
YFIRADEALYRAKSSGRNCVVSERITETAI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=37.3684210526316, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI145693134, Length=179, Percent_Identity=34.6368715083799, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=33.7349397590361, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=34.2105263157895, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=35.5932203389831, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1787816, Length=283, Percent_Identity=27.5618374558304, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=191, Percent_Identity=33.5078534031414, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=183, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=32.7485380116959, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=27.810650887574, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=28.476821192053, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787056, Length=161, Percent_Identity=31.055900621118, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38957; Mature: 38957

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMAKHLSETELSAKILRHAVPKMSELKVPVTPENYAVWYEYYKGINLDLKRAIDGLLSN
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
NVAFTPDVSSSLYKNYILINSPEIIENVQLETQVLINSLLAKIGSLSKDTSRFSDSLTEF
CCEECCCCHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DNALKQDPDPVVMQKLVKGITSELDEIIAVNGEMDKSLTSMNEEVIALKSEMVELRSVAM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSVNNRRAFDKEVISHIDSFNHSNFDSSLLVVDIDHFKRFNDIHGHLVGDKVLAYV
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHH
AQALKNGVKGDDFVARYGGEEFVILLPNTTQEEANIVAENLRQKIAERNLTIGKDKKLPL
HHHHHCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GNITVSVGVASLKAADDKDSYFIRADEALYRAKSSGRNCVVSERITETAI
CCEEEEECHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCEEEHHHHHHHCC
>Mature Secondary Structure
MDMAKHLSETELSAKILRHAVPKMSELKVPVTPENYAVWYEYYKGINLDLKRAIDGLLSN
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
NVAFTPDVSSSLYKNYILINSPEIIENVQLETQVLINSLLAKIGSLSKDTSRFSDSLTEF
CCEECCCCHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DNALKQDPDPVVMQKLVKGITSELDEIIAVNGEMDKSLTSMNEEVIALKSEMVELRSVAM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSVNNRRAFDKEVISHIDSFNHSNFDSSLLVVDIDHFKRFNDIHGHLVGDKVLAYV
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHH
AQALKNGVKGDDFVARYGGEEFVILLPNTTQEEANIVAENLRQKIAERNLTIGKDKKLPL
HHHHHCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GNITVSVGVASLKAADDKDSYFIRADEALYRAKSSGRNCVVSERITETAI
CCEEEEECHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA