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The map label for this gene is eptA [H]

Identifier: 167623886

GI number: 167623886

Start: 2350361

End: 2351980

Strand: Direct

Name: eptA [H]

Synonym: Shal_1956

Alternate gene names: 167623886

Gene position: 2350361-2351980 (Clockwise)

Preceding gene: 167623885

Following gene: 167623887

Centisome position: 44.97

GC content: 39.57

Gene sequence:

>1620_bases
GTGTTAGCACGTCTTAAAAATTTAAATAGCATTCAATTTACCGTCATACTTTCGATTTACTATGTATGCGTGTTTAATAT
TCCTCTTTTTCAAATAATCAAACAGGGCGTAGATAAACAAGCCGATGTCAATATGGCATTTATTGCCAGTATGCCTTTGT
TTCTGTTTTTCGCTCTGTCATTTTTATTTAGCCTTTTTAGTGTTAAGTATCTAGCAAAGCCATTTTTTATTATTTTGACG
TTGATGTCGTCGAGTGTCTTTTTTGCGGCATTTCAATATGGTGTGGTATTTGACTACGGCATGATTGAAAACACCTTTCA
GACCAATCAATCAGAAGCGACAACCTACCTTAATTGGGCGTCAGTTGTTAACTTCGTGGTGACAGGCATTATCCCCTCAC
TGTTTATCTATAAAGCTAACATTCAGTACAAGCCCATAACGAAAGAACTGCTGCACAAGGCTTTATTTATGCTGTCTATG
CTTGCAGGTATCGTCGTGATTGCTATGTTCTATTATCAAAATTACGTCGCATTTGGTCGAAATAACGACATCATGAAGCG
TTATATTATCCCAACATATTTTATTGGTTCAACTGTTAAGTATCTAAAAATTAACTACTTACAAGAGCCTTTAGCGTATA
AACAGATTGGTTTGGACGCCAAAAACACCACAGACAAAGCCAAGCCTAATTTAGTCATTTTGGTGGTAGGAGAAACGGCT
AGGGCGGCCAATTATGCATATTATGGTTACGACAGACCGACCAATGCGCATACTAAAGAGCAGGGGATAGTGGTGTTTAA
AGACACCCATTCATGTGGTACTGCTACAGCCGTCTCTTTGCCTTGCATGTTTTCTAGAATGGATCGTGATAATTATAATT
CGCGTCGAGAAAGAGCCCAAGACAGCGCTATCGATGTGTTAAACCATGCAGGGATCCAACTTGAATGGCTCGACAATGAC
AGTGGCTGTAAAGGTGTTTGCCAGAATATTGAGAGTATCACAATAGATCATAATAGCGATCCTAAGCTGTGTAACGGTGA
GTATTGTTACGATCAAGTGTTGCTTAATGAGTTAGGTAAGCGGCTTAAAACCATTAAAGATGAAGACACATTATTAGTGC
TACACATCATTGGTTCTCATGGGCCGACCTATTATCTACGTTATCCGGAGAAGTATCGTCAGTTTGTGCCTGACTGCCAA
CGTAGCGATATTCAAAACTGCAGCCATGAAGAGTTGGTTAACACTTACGATAATACTATTTTATATACCGACTTTATCCT
CTCACAGGTCGTTAATAAGCTAAAAGCAACAAGCGCGCAATTCGATACTGCAATGCTCTATATATCAGATCATGGTGAGT
CATTAGGCGAAAAAGGCATGTATCTTCATGGTACGCCTTATTCATTTGCGCCCGATGAGCAAACAAAAGTGCCTTTCTTG
GGCTGGTTCTCAAGCGGTTTTGCGGAGCAAAATCACTTAAACCTTGCTTGCTTAGCAAAAGAAGCTAACAAGGGCGGTTT
CTCTCACGACAATTTATTCGATAGCTTACTTGGTTTGATGAATGTCCGTAGTGAGGTTTATCGGCCAAAATCTGATATTT
TCGCGAGTTGTCGAAAATAA

Upstream 100 bases:

>100_bases
TTAGCTGTTTTATTTAGGTGCTATTATATGGCCATATCCGCCGATAACGGCTATAGGCAAGGAGAGCCCAAATAATCTTA
AAAATAAGTGAGTAAACTAG

Downstream 100 bases:

>100_bases
AATCTAATTTATCTCTAAAAAGGGATGGAAACATCTTCATTTTTGTTTAAAGTATTGATAAGTAAAGCTATCAAGATGGA
GTGACTTTTAACATGGATAT

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLARLKNLNSIQFTVILSIYYVCVFNIPLFQIIKQGVDKQADVNMAFIASMPLFLFFALSFLFSLFSVKYLAKPFFIILT
LMSSSVFFAAFQYGVVFDYGMIENTFQTNQSEATTYLNWASVVNFVVTGIIPSLFIYKANIQYKPITKELLHKALFMLSM
LAGIVVIAMFYYQNYVAFGRNNDIMKRYIIPTYFIGSTVKYLKINYLQEPLAYKQIGLDAKNTTDKAKPNLVILVVGETA
RAANYAYYGYDRPTNAHTKEQGIVVFKDTHSCGTATAVSLPCMFSRMDRDNYNSRRERAQDSAIDVLNHAGIQLEWLDND
SGCKGVCQNIESITIDHNSDPKLCNGEYCYDQVLLNELGKRLKTIKDEDTLLVLHIIGSHGPTYYLRYPEKYRQFVPDCQ
RSDIQNCSHEELVNTYDNTILYTDFILSQVVNKLKATSAQFDTAMLYISDHGESLGEKGMYLHGTPYSFAPDEQTKVPFL
GWFSSGFAEQNHLNLACLAKEANKGGFSHDNLFDSLLGLMNVRSEVYRPKSDIFASCRK

Sequences:

>Translated_539_residues
MLARLKNLNSIQFTVILSIYYVCVFNIPLFQIIKQGVDKQADVNMAFIASMPLFLFFALSFLFSLFSVKYLAKPFFIILT
LMSSSVFFAAFQYGVVFDYGMIENTFQTNQSEATTYLNWASVVNFVVTGIIPSLFIYKANIQYKPITKELLHKALFMLSM
LAGIVVIAMFYYQNYVAFGRNNDIMKRYIIPTYFIGSTVKYLKINYLQEPLAYKQIGLDAKNTTDKAKPNLVILVVGETA
RAANYAYYGYDRPTNAHTKEQGIVVFKDTHSCGTATAVSLPCMFSRMDRDNYNSRRERAQDSAIDVLNHAGIQLEWLDND
SGCKGVCQNIESITIDHNSDPKLCNGEYCYDQVLLNELGKRLKTIKDEDTLLVLHIIGSHGPTYYLRYPEKYRQFVPDCQ
RSDIQNCSHEELVNTYDNTILYTDFILSQVVNKLKATSAQFDTAMLYISDHGESLGEKGMYLHGTPYSFAPDEQTKVPFL
GWFSSGFAEQNHLNLACLAKEANKGGFSHDNLFDSLLGLMNVRSEVYRPKSDIFASCRK
>Mature_539_residues
MLARLKNLNSIQFTVILSIYYVCVFNIPLFQIIKQGVDKQADVNMAFIASMPLFLFFALSFLFSLFSVKYLAKPFFIILT
LMSSSVFFAAFQYGVVFDYGMIENTFQTNQSEATTYLNWASVVNFVVTGIIPSLFIYKANIQYKPITKELLHKALFMLSM
LAGIVVIAMFYYQNYVAFGRNNDIMKRYIIPTYFIGSTVKYLKINYLQEPLAYKQIGLDAKNTTDKAKPNLVILVVGETA
RAANYAYYGYDRPTNAHTKEQGIVVFKDTHSCGTATAVSLPCMFSRMDRDNYNSRRERAQDSAIDVLNHAGIQLEWLDND
SGCKGVCQNIESITIDHNSDPKLCNGEYCYDQVLLNELGKRLKTIKDEDTLLVLHIIGSHGPTYYLRYPEKYRQFVPDCQ
RSDIQNCSHEELVNTYDNTILYTDFILSQVVNKLKATSAQFDTAMLYISDHGESLGEKGMYLHGTPYSFAPDEQTKVPFL
GWFSSGFAEQNHLNLACLAKEANKGGFSHDNLFDSLLGLMNVRSEVYRPKSDIFASCRK

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=528, Percent_Identity=36.7424242424242, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI87082286, Length=468, Percent_Identity=27.5641025641026, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI87082223, Length=455, Percent_Identity=25.4945054945055, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1787035, Length=280, Percent_Identity=24.6428571428571, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61273; Mature: 61273

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARLKNLNSIQFTVILSIYYVCVFNIPLFQIIKQGVDKQADVNMAFIASMPLFLFFALS
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FLFSLFSVKYLAKPFFIILTLMSSSVFFAAFQYGVVFDYGMIENTFQTNQSEATTYLNWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHCCCCCHHHHHHHHH
SVVNFVVTGIIPSLFIYKANIQYKPITKELLHKALFMLSMLAGIVVIAMFYYQNYVAFGR
HHHHHHHHHHHHHHEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
NNDIMKRYIIPTYFIGSTVKYLKINYLQEPLAYKQIGLDAKNTTDKAKPNLVILVVGETA
CCCHHHHHHCHHHHHCCCEEHEEHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
RAANYAYYGYDRPTNAHTKEQGIVVFKDTHSCGTATAVSLPCMFSRMDRDNYNSRRERAQ
CCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
DSAIDVLNHAGIQLEWLDNDSGCKGVCQNIESITIDHNSDPKLCNGEYCYDQVLLNELGK
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
RLKTIKDEDTLLVLHIIGSHGPTYYLRYPEKYRQFVPDCQRSDIQNCSHEELVNTYDNTI
HHHHCCCCCCEEEEEEECCCCCEEEEECCHHHHHHCCCCHHHHHCCCCHHHHHHHHCCEE
LYTDFILSQVVNKLKATSAQFDTAMLYISDHGESLGEKGMYLHGTPYSFAPDEQTKVPFL
EHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEE
GWFSSGFAEQNHLNLACLAKEANKGGFSHDNLFDSLLGLMNVRSEVYRPKSDIFASCRK
EEHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MLARLKNLNSIQFTVILSIYYVCVFNIPLFQIIKQGVDKQADVNMAFIASMPLFLFFALS
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FLFSLFSVKYLAKPFFIILTLMSSSVFFAAFQYGVVFDYGMIENTFQTNQSEATTYLNWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHCCCCCHHHHHHHHH
SVVNFVVTGIIPSLFIYKANIQYKPITKELLHKALFMLSMLAGIVVIAMFYYQNYVAFGR
HHHHHHHHHHHHHHEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
NNDIMKRYIIPTYFIGSTVKYLKINYLQEPLAYKQIGLDAKNTTDKAKPNLVILVVGETA
CCCHHHHHHCHHHHHCCCEEHEEHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
RAANYAYYGYDRPTNAHTKEQGIVVFKDTHSCGTATAVSLPCMFSRMDRDNYNSRRERAQ
CCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
DSAIDVLNHAGIQLEWLDNDSGCKGVCQNIESITIDHNSDPKLCNGEYCYDQVLLNELGK
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
RLKTIKDEDTLLVLHIIGSHGPTYYLRYPEKYRQFVPDCQRSDIQNCSHEELVNTYDNTI
HHHHCCCCCCEEEEEEECCCCCEEEEECCHHHHHHCCCCHHHHHCCCCHHHHHHHHCCEE
LYTDFILSQVVNKLKATSAQFDTAMLYISDHGESLGEKGMYLHGTPYSFAPDEQTKVPFL
EHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEE
GWFSSGFAEQNHLNLACLAKEANKGGFSHDNLFDSLLGLMNVRSEVYRPKSDIFASCRK
EEHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]