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The map label for this gene is mnmE [H]

Identifier: 167623345

GI number: 167623345

Start: 1707442

End: 1708683

Strand: Direct

Name: mnmE [H]

Synonym: Shal_1414

Alternate gene names: 167623345

Gene position: 1707442-1708683 (Clockwise)

Preceding gene: 167623344

Following gene: 167623346

Centisome position: 32.67

GC content: 44.2

Gene sequence:

>1242_bases
ATGTGTCAAGGTGTTAACCCCGAAACCAGCCAAGGTCAAATAGCCCCCAGAGGGATGCGCTATCAAATTGCAATATTAGG
GAGGCGCAACGCTGGTAAATCATCATTACTGAATATGATAGTAGGCCAACACGTTGCCATTGTTTCTGATATAAAAGGCA
CAACCACCGACGCAGTCGCTAAGCCATACGAGCTATTACCGTTAGGGCCGGTCACTTTTTTCGATACTGCAGGTATTGAT
GATAATGGAGCACTTGGTGAGCAAAGAGTAAAAGCAACGCGACATGTACTTTACCGCGCCGATATGGCGCTATTAGTTGC
TGATGATCAGGGCCTAACCGAGCATGAGTTTGCGCTGATTGAAGAAATGACAGCATTAAAACTCCCATTCTTAATCGTAT
TTAACAAAGTGGATATTTGCATGCCAACGGCTGAAGATATCGGCTTTTGTCGACAAAGAAAGCTGCCTTTTATTGCGGTA
TCTGCAGCGAAAGGGCTGGGAGATAAAGAAGTTAAAAGACTAATTTCCGAGCTCGTACCAGCAAATCTTAAAGAGCAGCC
TCCTCTTGCAGGCGATCTCTACAATGAAGGTGCACATATCTTATGTGTAGTCCCCATTGATACAGCGGCTCCGAAAGGGC
GACTAATCCTGCCTCAGGTTCAAGTACTGAGAGAGGCATTAGATAATAATTGCATTGCGACCGTAGTTAAAGAATCTCAG
CTTGCACAGGTTCTAACTCAATTTAGTTCCCCGCCAGCACTGGTGATCTCCGATGCTCAAGTGATCAAACAGGTCGATAG
TATCGTGCCAGCCACTATTCCATTAACCACATTTTCAACTTTATTTGCGCGTTTTAAAGGCGAGTTACCACCAATGGTTG
CCGGTGCCAATAGCTTAGATAAGTTGCAAGATGGTTGTAAGATTTTAATCTGTGAAGCATGTAGTCATCATGCCCAAGAT
GATGATATTGGTCGAGTAAAGTTGCCAAATTGGATCCGTAGCTATAGCGGGAAAAACATTACTTTTGACGTGATAAGTGG
TCATGATTTCCCTGACAACCTAGAAGAGTATGCGCTAGTGGTTCATTGTGGTGCATGCATGTTCAATCGTACCGAAATGC
TAAGACGGATCCGTGAATGCGAACGTAGAAACGTGCCAATCACCAACTATGGCGTCGCAATTTCAAAACTTCAGGGAGTG
TTACCACGGATCATTCAACCGTTAATAAGTGAAGCCAAATAA

Upstream 100 bases:

>100_bases
ACCAGTGATATTCAGCAAAGAGTAGCGGCCATATTTCAACAAATACAACACCATGGCTTACAACCTAACGGTAGCCGTGG
TGACTCAAGAAGGTATTAAT

Downstream 100 bases:

>100_bases
ATCAACAACGGCATCGAATAGAAGTCACAGTCAACACAGTCATTTACTTTCACATGGATGTGAACAGATCCAACAAACAC
AGACTAATTCCAGATAGGCT

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MCQGVNPETSQGQIAPRGMRYQIAILGRRNAGKSSLLNMIVGQHVAIVSDIKGTTTDAVAKPYELLPLGPVTFFDTAGID
DNGALGEQRVKATRHVLYRADMALLVADDQGLTEHEFALIEEMTALKLPFLIVFNKVDICMPTAEDIGFCRQRKLPFIAV
SAAKGLGDKEVKRLISELVPANLKEQPPLAGDLYNEGAHILCVVPIDTAAPKGRLILPQVQVLREALDNNCIATVVKESQ
LAQVLTQFSSPPALVISDAQVIKQVDSIVPATIPLTTFSTLFARFKGELPPMVAGANSLDKLQDGCKILICEACSHHAQD
DDIGRVKLPNWIRSYSGKNITFDVISGHDFPDNLEEYALVVHCGACMFNRTEMLRRIRECERRNVPITNYGVAISKLQGV
LPRIIQPLISEAK

Sequences:

>Translated_413_residues
MCQGVNPETSQGQIAPRGMRYQIAILGRRNAGKSSLLNMIVGQHVAIVSDIKGTTTDAVAKPYELLPLGPVTFFDTAGID
DNGALGEQRVKATRHVLYRADMALLVADDQGLTEHEFALIEEMTALKLPFLIVFNKVDICMPTAEDIGFCRQRKLPFIAV
SAAKGLGDKEVKRLISELVPANLKEQPPLAGDLYNEGAHILCVVPIDTAAPKGRLILPQVQVLREALDNNCIATVVKESQ
LAQVLTQFSSPPALVISDAQVIKQVDSIVPATIPLTTFSTLFARFKGELPPMVAGANSLDKLQDGCKILICEACSHHAQD
DDIGRVKLPNWIRSYSGKNITFDVISGHDFPDNLEEYALVVHCGACMFNRTEMLRRIRECERRNVPITNYGVAISKLQGV
LPRIIQPLISEAK
>Mature_413_residues
MCQGVNPETSQGQIAPRGMRYQIAILGRRNAGKSSLLNMIVGQHVAIVSDIKGTTTDAVAKPYELLPLGPVTFFDTAGID
DNGALGEQRVKATRHVLYRADMALLVADDQGLTEHEFALIEEMTALKLPFLIVFNKVDICMPTAEDIGFCRQRKLPFIAV
SAAKGLGDKEVKRLISELVPANLKEQPPLAGDLYNEGAHILCVVPIDTAAPKGRLILPQVQVLREALDNNCIATVVKESQ
LAQVLTQFSSPPALVISDAQVIKQVDSIVPATIPLTTFSTLFARFKGELPPMVAGANSLDKLQDGCKILICEACSHHAQD
DDIGRVKLPNWIRSYSGKNITFDVISGHDFPDNLEEYALVVHCGACMFNRTEMLRRIRECERRNVPITNYGVAISKLQGV
LPRIIQPLISEAK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI2367268, Length=171, Percent_Identity=36.2573099415205, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45156; Mature: 45156

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQGVNPETSQGQIAPRGMRYQIAILGRRNAGKSSLLNMIVGQHVAIVSDIKGTTTDAVA
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCHHEEEECCCCCCHHHHC
KPYELLPLGPVTFFDTAGIDDNGALGEQRVKATRHVLYRADMALLVADDQGLTEHEFALI
CCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHH
EEMTALKLPFLIVFNKVDICMPTAEDIGFCRQRKLPFIAVSAAKGLGDKEVKRLISELVP
HHHHHHHCCEEEEECCCCEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCC
ANLKEQPPLAGDLYNEGAHILCVVPIDTAAPKGRLILPQVQVLREALDNNCIATVVKESQ
CCCCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCEECHHHHHHHHHHCCCCEEEHHHHHH
LAQVLTQFSSPPALVISDAQVIKQVDSIVPATIPLTTFSTLFARFKGELPPMVAGANSLD
HHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCCCHHH
KLQDGCKILICEACSHHAQDDDIGRVKLPNWIRSYSGKNITFDVISGHDFPDNLEEYALV
HHHCCCEEEEEEHHCCCCCCCCCCCEECCHHHHHCCCCCEEEEEECCCCCCCCHHHHEEH
VHCGACMFNRTEMLRRIRECERRNVPITNYGVAISKLQGVLPRIIQPLISEAK
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCQGVNPETSQGQIAPRGMRYQIAILGRRNAGKSSLLNMIVGQHVAIVSDIKGTTTDAVA
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCHHEEEECCCCCCHHHHC
KPYELLPLGPVTFFDTAGIDDNGALGEQRVKATRHVLYRADMALLVADDQGLTEHEFALI
CCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHH
EEMTALKLPFLIVFNKVDICMPTAEDIGFCRQRKLPFIAVSAAKGLGDKEVKRLISELVP
HHHHHHHCCEEEEECCCCEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCC
ANLKEQPPLAGDLYNEGAHILCVVPIDTAAPKGRLILPQVQVLREALDNNCIATVVKESQ
CCCCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCEECHHHHHHHHHHCCCCEEEHHHHHH
LAQVLTQFSSPPALVISDAQVIKQVDSIVPATIPLTTFSTLFARFKGELPPMVAGANSLD
HHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCCCHHH
KLQDGCKILICEACSHHAQDDDIGRVKLPNWIRSYSGKNITFDVISGHDFPDNLEEYALV
HHHCCCEEEEEEHHCCCCCCCCCCCEECCHHHHHCCCCCEEEEEECCCCCCCCHHHHEEH
VHCGACMFNRTEMLRRIRECERRNVPITNYGVAISKLQGVLPRIIQPLISEAK
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]