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The map label for this gene is hisS

Identifier: 167623300

GI number: 167623300

Start: 1643194

End: 1644468

Strand: Direct

Name: hisS

Synonym: Shal_1368

Alternate gene names: 167623300

Gene position: 1643194-1644468 (Clockwise)

Preceding gene: 167623299

Following gene: 167623301

Centisome position: 31.44

GC content: 46.59

Gene sequence:

>1275_bases
GTGGCAAAACAGATCCAAGCGATTCGCGGAATGAATGACATTCTGCCTACTCAAAGTCCTTTATGGCAAAAACTTGAAAC
CGTATTACGTGAGACTGTGGGTTCATACGGTTACAGCGAAATTCGTACTCCTATCGTTGAAAGTACAGATCTCTTTAAGC
GCTCTATCGGTGAAGTGACTGATATCGTTGAAAAAGAGATGTATACCTTTGAAGATAGAAACGGTGACAGCTTAACGCTG
CGTCCAGAAGGCACTGCCTCAACTGTTCGCGCGGGTAATGAGCACGGTTTATTATACAACCAAGAGCAGCGTCTTTGGTA
TATGGGCCCAATGTTCCGCCACGAACGCCCTCAAAAAGGTCGTTACCGTCAATTCCACCAGTTTGGTGTTGAAGTCTATG
GCATCCCAACGGCTGATATCGATGCAGAAGTCTTAATGCTATCTGCCAAGCTATGGGAAAAACTCGGTATTACCGAGCAT
GTGACCTTAGAGCTAAATACCTTAGGTGATGTTGAAGAACGCGCCGCTTACCGTGATGCCTTAATTGCTTTCTTAGAGCA
ACACAAAGAGGTGTTAGACGAAGATAGTCAGCGTCGTATGTACAGCAACCCATTACGTGTACTCGATTCTAAAAATGCCG
ATGTACAGGCGCTTTTGGCCGATGCCCCAGTGTTAATGGACTACTTTGGTGAAGACACGCGCTCACATTTCTCACATTTA
TGTGAACTCTTAGAGGCTGTCGGTATCCAATACACAATCAATCCTCGCCTAGTGCGCGGTTTAGATTATTATAATCGCAC
GGTTTTTGAGTGGGTCACATCAAGCTTAGGTTCACAAGGCACAGTTTTAGCCGGTGGACGTTACGACGGGCTTGTGGGTC
AGCTTGGCGGTAAGCCAACTCCAGCTGTAGGTTTTGCAATGGGCCTTGAGCGCATCGTATTACTGCTAGAAACACTTGAA
CTAGACAAGGACATTGGTCCAAGCGTCGACGTCTACGTGACAGCAATGGGTGACAATTGCCGCGTTGAAGCGATTAAGAT
CGCTCAAGAGCTAAGAGCAAGCTTGCCAACTGCTAAAGTGATGAGCCATTGTGGTGGCGGTAACTTTAAGAAGCAGATGA
AGCGTGCCGATAAGAGTGGTGCCACTGTTGCGCTAGTGATTGGCGAAGATGAACTTGCCAATAACCAAGTCGCAGTGAAG
CATCTTCGCGAAGATAAAGCACAAGAATTAGTTGCCCGTGATGCGTTGGCGACTTATATTGCAGAACTGATTTAA

Upstream 100 bases:

>100_bases
ATTTTTTATGGGTGAATGCACCTGTTGTACGTTTAGTCTCGAAACGCCTATAATGTGAGGCGTTTTTTAATTTTATATAG
CAAATTTGACGAGTCGAATA

Downstream 100 bases:

>100_bases
AAGGGGAAGACTAAGTGGAAATCTATAGCACAGAAGAGCAACAAGTAGATGCTATCAAACAGTTCTGGAAAGATTACGGC
ACATCAGTTGTTGTTGGCGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKQIQAIRGMNDILPTQSPLWQKLETVLRETVGSYGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEH
VTLELNTLGDVEERAAYRDALIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPTPAVGFAMGLERIVLLLETLE
LDKDIGPSVDVYVTAMGDNCRVEAIKIAQELRASLPTAKVMSHCGGGNFKKQMKRADKSGATVALVIGEDELANNQVAVK
HLREDKAQELVARDALATYIAELI

Sequences:

>Translated_424_residues
MAKQIQAIRGMNDILPTQSPLWQKLETVLRETVGSYGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEH
VTLELNTLGDVEERAAYRDALIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPTPAVGFAMGLERIVLLLETLE
LDKDIGPSVDVYVTAMGDNCRVEAIKIAQELRASLPTAKVMSHCGGGNFKKQMKRADKSGATVALVIGEDELANNQVAVK
HLREDKAQELVARDALATYIAELI
>Mature_423_residues
AKQIQAIRGMNDILPTQSPLWQKLETVLRETVGSYGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTLR
PEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKGRYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEHV
TLELNTLGDVEERAAYRDALIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHLC
ELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPTPAVGFAMGLERIVLLLETLEL
DKDIGPSVDVYVTAMGDNCRVEAIKIAQELRASLPTAKVMSHCGGGNFKKQMKRADKSGATVALVIGEDELANNQVAVKH
LREDKAQELVARDALATYIAELI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=457, Percent_Identity=24.72647702407, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI15029520, Length=463, Percent_Identity=23.7580993520518, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=67.688679245283, Blast_Score=603, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6325290, Length=462, Percent_Identity=24.2424242424242, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SHEHH (B0TLI5)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673594.1
- ProteinModelPortal:   B0TLI5
- SMR:   B0TLI5
- GeneID:   5907016
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1368
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   SHAL458817:SHAL_1368-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47430; Mature: 47298

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIQAIRGMNDILPTQSPLWQKLETVLRETVGSYGYSEIRTPIVESTDLFKRSIGEVT
CCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEHVTLELNTLGDVEERAAYRDA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
LIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
HHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHHHCCHHHHHHHHH
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPT
HHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLETLELDKDIGPSVDVYVTAMGDNCRVEAIKIAQELRASLPTAKV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHH
MSHCGGGNFKKQMKRADKSGATVALVIGEDELANNQVAVKHLREDKAQELVARDALATYI
HHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELI
HHHC
>Mature Secondary Structure 
AKQIQAIRGMNDILPTQSPLWQKLETVLRETVGSYGYSEIRTPIVESTDLFKRSIGEVT
CCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQFGVEVYGIPTADIDAEVLMLSAKLWEKLGITEHVTLELNTLGDVEERAAYRDA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHH
LIAFLEQHKEVLDEDSQRRMYSNPLRVLDSKNADVQALLADAPVLMDYFGEDTRSHFSHL
HHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCHHHHHHCCHHHHHHHCCHHHHHHHHH
CELLEAVGIQYTINPRLVRGLDYYNRTVFEWVTSSLGSQGTVLAGGRYDGLVGQLGGKPT
HHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCC
PAVGFAMGLERIVLLLETLELDKDIGPSVDVYVTAMGDNCRVEAIKIAQELRASLPTAKV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHH
MSHCGGGNFKKQMKRADKSGATVALVIGEDELANNQVAVKHLREDKAQELVARDALATYI
HHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELI
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA