Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 167623296

GI number: 167623296

Start: 1638993

End: 1640114

Strand: Direct

Name: rlmN [H]

Synonym: Shal_1364

Alternate gene names: 167623296

Gene position: 1638993-1640114 (Clockwise)

Preceding gene: 167623293

Following gene: 167623297

Centisome position: 31.36

GC content: 44.21

Gene sequence:

>1122_bases
ATGAGTGAAAAGAAGATCAATTTATTGGATCTTGATCGTAAAGCATTAAGAGTGCTATTTACCGAAATGGGTGAAAAACC
GTTTCGTGCTGATCAATTGATGAAATGGATTTATCATTTTGGCGTTAGTGACTTCGAAGAGATGACCAATATTAACAAAG
TGTTGCGAGCAAAGCTTGCCGCTAAATGTGAAATTGTCGCACCTGAAATATCAAGCTACCAAAAGTCAGTTGATGGCACG
ATTAAGTTCGCCATCAATGTAGGTGATGGCCAAGAAGTTGAAACGGTTTACATCCCAGAAGATGACCGTGCAACCTTGTG
CGTATCGTCACAAGTCGGTTGTGCGCTTGAATGTACTTTCTGCTCTACAGCGCAGCAAGGCTTTAACCGTAACCTGACTG
TTGCTGAAATCGTTGGCCAGATTTGGCGTGTTGCCGACTTTATCGGTTTCGTTAAAGATACTGGCGAGCGTCCGATTACT
AACGTCGTTATGATGGGCATGGGCGAGCCACTGCTTAACCTAAAGAACGTTATCCCTGCGATGGATATCATGCTTGATGA
CTTTGGTTTTAGTCTATCTAAGCGCCGCGTAACGCTATCAACATCAGGCGTTGTTCCTGCATTAGATAAACTCGGTGATG
CACTTGATGTCGCATTAGCCGTGAGTATCCACGCGCCTAATGATGAGCTGCGTGATGTATTAGTGCCAGTTAACAAAAAG
TATCCTTTAGAAGAGTTCCTTGGGGGGATTCGCCGCTATATTGCTAAGTCGAATGCTAACCGTGGTAGAGTGACGGTTGA
ATATGTCATGCTCGATCACATTAATGACAGCACCGACCAAGCGCATGAGCTGGCCAAGTTGATGAAAGATACCCCTTGTA
AGGTAAACTTGATCCCATTTAATCCTTATCCTGGTTCTCCATACGGGCGTTCATCTAATTCTCGTATCGATAGATTCTCG
AAAGTATTGATGGAATATGGTCTTACGGTTATCGTGCGTAAGACCCGCGGCGATGATATCGATGCAGCATGTGGCCAGCT
AGCCGGTGATATTCGCGATAGAACCAAGCGTTTAGCGAAAAAACAAATGCAAGAGAGCCAAATTTCAGTCACAATGAACT
AA

Upstream 100 bases:

>100_bases
AGGTTTTTAACTTTGGTGATAGCCTGCTGGCTAATATTTGTTAAGATCTGTATAATTCGCCGCCCGCAAGCAGGTTTTAG
GTTTTTTGAGGTTAGACAAA

Downstream 100 bases:

>100_bases
CTCTTTGTATCTACAGGTAGATGGGCGCAACAATGAATCAGAGTAAGGTGGTGTTGCCACTGGTATTAATATTGTCATCT
ATGGTGACAACGGGGTGCGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEKKINLLDLDRKALRVLFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLAAKCEIVAPEISSYQKSVDGT
IKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPIT
NVVMMGMGEPLLNLKNVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDVLVPVNKK
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKQMQESQISVTMN

Sequences:

>Translated_373_residues
MSEKKINLLDLDRKALRVLFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLAAKCEIVAPEISSYQKSVDGT
IKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPIT
NVVMMGMGEPLLNLKNVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDVLVPVNKK
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKQMQESQISVTMN
>Mature_372_residues
SEKKINLLDLDRKALRVLFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLAAKCEIVAPEISSYQKSVDGTI
KFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPITN
VVMMGMGEPLLNLKNVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDVLVPVNKKY
PLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSK
VLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKQMQESQISVTMN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=74.3169398907104, Blast_Score=590, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41582; Mature: 41450

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKINLLDLDRKALRVLFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLA
CCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AKCEIVAPEISSYQKSVDGTIKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTF
HCEEEECCCHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPITNVVMMGMGEPLLNLKNVIPA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDVLVPVNKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCHHEEEEEEEEECCCHHHHHHCCCCCCC
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQMQESQISVTMN
HHHHHCCEEEEEC
>Mature Secondary Structure 
SEKKINLLDLDRKALRVLFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLA
CCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AKCEIVAPEISSYQKSVDGTIKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTF
HCEEEECCCHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPITNVVMMGMGEPLLNLKNVIPA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDVLVPVNKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCHHEEEEEEEEECCCHHHHHHCCCCCCC
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQMQESQISVTMN
HHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA