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The map label for this gene is mazG [H]

Identifier: 167623154

GI number: 167623154

Start: 1484116

End: 1484919

Strand: Direct

Name: mazG [H]

Synonym: Shal_1220

Alternate gene names: 167623154

Gene position: 1484116-1484919 (Clockwise)

Preceding gene: 167623153

Following gene: 167623155

Centisome position: 28.39

GC content: 45.65

Gene sequence:

>804_bases
ATGACAAGCTTAGTCGGCGATACGACCCCGCTACTTAATATTATGCAAAAACTGCGTGACCCAGAAACAGGTTGTCCCTG
GGACAGGGCGCAAAGTTTTCAAACCATCGTACCTTTTACCATAGAGGAAGCCTACGAGGTCGCCGATACCATTGAGCGTA
TGGCGCTTGATGAGTTGCCTGACGAGTTAGGGGATCTGCTATTTCAGGTGGTATTTTACTGTCAACTGGGCAAAGAGCAG
GGGCTTTTTGATTTCAATACCGTGGTTGAACGTATATGCGATAAATTAACTCGCCGTCATCCCCATGTATTTGAAGGATT
AGCGACAACGAGTACAGATGAGATCAAGCAAAATTGGGAACAGTTAAAAGCTATCGAGCGTAGTGAAAGAGCACTCGACT
CTGTACTAGACGATATTCCGCTGAGTTTACCCGCGCTGTCGCGTTCGATAAAAATTCAAAAACGTGTCGCTCGAGTGGGT
TTTGACTGGGCTGAACTGCCGCCCGTGGTGGCAAAAATCCATGAAGAGATTGATGAAGTCATTTTTGAAGTCGAGCAAGA
AACGGTAGATGTGCAAAAAGTGCAAGACGAAATGGGCGACTTATTGTTTGCCGTTGTGAACCTTGCAAGACACTTAGGTG
TTGAGCCTGAAAAGGCATTAAGGCAAGCAAATCATAAGTTTGAAAGAAGGTTTAGGGGTGTTGAAGCCTATGCTCAAGCG
GATGGCCGTGCCATGAATGAGCATACATTAGAGGAGCTCGATGGCTACTGGGATAGCGTTAAACAGGATGAGAGAAATAA
ATAA

Upstream 100 bases:

>100_bases
ATGGTTGCCGTTTTTATATGGTTTACTTTTAAAGTAAACGTTGGCAAACATGTATTCAGCCCTGTGCGAAATAAACGATA
TCGGAAAGTAGGAAAATAAA

Downstream 100 bases:

>100_bases
CTTAGATTTAAAGCTAATTGTAGTTTGTCGAATCGCAGTTGCTTTGGTATAATATTGCCCCGTCCTAGTGCTTTCTTACA
AGCACGTATTTCCAACTCAT

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTSLVGDTTPLLNIMQKLRDPETGCPWDRAQSFQTIVPFTIEEAYEVADTIERMALDELPDELGDLLFQVVFYCQLGKEQ
GLFDFNTVVERICDKLTRRHPHVFEGLATTSTDEIKQNWEQLKAIERSERALDSVLDDIPLSLPALSRSIKIQKRVARVG
FDWAELPPVVAKIHEEIDEVIFEVEQETVDVQKVQDEMGDLLFAVVNLARHLGVEPEKALRQANHKFERRFRGVEAYAQA
DGRAMNEHTLEELDGYWDSVKQDERNK

Sequences:

>Translated_267_residues
MTSLVGDTTPLLNIMQKLRDPETGCPWDRAQSFQTIVPFTIEEAYEVADTIERMALDELPDELGDLLFQVVFYCQLGKEQ
GLFDFNTVVERICDKLTRRHPHVFEGLATTSTDEIKQNWEQLKAIERSERALDSVLDDIPLSLPALSRSIKIQKRVARVG
FDWAELPPVVAKIHEEIDEVIFEVEQETVDVQKVQDEMGDLLFAVVNLARHLGVEPEKALRQANHKFERRFRGVEAYAQA
DGRAMNEHTLEELDGYWDSVKQDERNK
>Mature_266_residues
TSLVGDTTPLLNIMQKLRDPETGCPWDRAQSFQTIVPFTIEEAYEVADTIERMALDELPDELGDLLFQVVFYCQLGKEQG
LFDFNTVVERICDKLTRRHPHVFEGLATTSTDEIKQNWEQLKAIERSERALDSVLDDIPLSLPALSRSIKIQKRVARVGF
DWAELPPVVAKIHEEIDEVIFEVEQETVDVQKVQDEMGDLLFAVVNLARHLGVEPEKALRQANHKFERRFRGVEAYAQAD
GRAMNEHTLEELDGYWDSVKQDERNK

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=254, Percent_Identity=57.8740157480315, Blast_Score=300, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30683; Mature: 30552

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLVGDTTPLLNIMQKLRDPETGCPWDRAQSFQTIVPFTIEEAYEVADTIERMALDELP
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
DELGDLLFQVVFYCQLGKEQGLFDFNTVVERICDKLTRRHPHVFEGLATTSTDEIKQNWE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHH
QLKAIERSERALDSVLDDIPLSLPALSRSIKIQKRVARVGFDWAELPPVVAKIHEEIDEV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHH
IFEVEQETVDVQKVQDEMGDLLFAVVNLARHLGVEPEKALRQANHKFERRFRGVEAYAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DGRAMNEHTLEELDGYWDSVKQDERNK
CCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSLVGDTTPLLNIMQKLRDPETGCPWDRAQSFQTIVPFTIEEAYEVADTIERMALDELP
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
DELGDLLFQVVFYCQLGKEQGLFDFNTVVERICDKLTRRHPHVFEGLATTSTDEIKQNWE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHH
QLKAIERSERALDSVLDDIPLSLPALSRSIKIQKRVARVGFDWAELPPVVAKIHEEIDEV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHH
IFEVEQETVDVQKVQDEMGDLLFAVVNLARHLGVEPEKALRQANHKFERRFRGVEAYAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DGRAMNEHTLEELDGYWDSVKQDERNK
CCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]