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The map label for this gene is rumA

Identifier: 167623152

GI number: 167623152

Start: 1480217

End: 1481554

Strand: Direct

Name: rumA

Synonym: Shal_1218

Alternate gene names: 167623152

Gene position: 1480217-1481554 (Clockwise)

Preceding gene: 167623149

Following gene: 167623153

Centisome position: 28.32

GC content: 45.81

Gene sequence:

>1338_bases
ATGGCACAATTTTTTAAAGCAAAACCAAATACATCTAAACAATTATCATCAAAATTGTCATTAAAGGTGACTCAGCTAGA
TCACCTCGGTGCTGGTATCGCTCACCACGATGGCAAGATCGTATTTATCAATGGTGCGTTGCCAGGTGAGACGGTATCGG
TGCAATTGACTGAGCAAAAGAAAAAGTTTGCTCGAGCTAAGTTACTTAAAATTGATAAAGCCTCCGCGCAGCGGGTTTCA
CCTCTTTGCCCACATTTCGACAAATGTGGTGGCTGTGATCTGCAGCACCTTGATATCGATGCACAGCGTAAGCATAAGGC
CAGTACCTTAGTCGATTTGGTGAATAAGTTTGCTCAAACCCAGGCTGGTGAAGTATGTGACACCCTAAGTGATAACGCTT
GGCATTATCGCCGCCGAGCCCGTTTAGCGACCTGGTTTGATAAAAATACCAAGCACATAAGCCTTGGATTTAGAGCCAGT
AGCAGCAGTGACGTGGTTGAGATCCAATCTTGCGCTGTGTTAGCCGAGCCGTTGTCGGCGTTAATTCCAGACTTGGCATT
TATGCTTAACCAGTTAAGCGGTAAGAAAGCCCTAGGCCACGTTGAGCTGACTCAAGCCGATAACGGTAACTTTGTGGTGC
TGCGTGTGACTAAGGCGTTATCGGATAAAGATAAGGCTAGGTTAGTTGAATTTGCGAATAAGCATCAGGTCATAGTGCTG
TTGCAAGATGATGATGCTCAGTGCGAGCACCTAAATGGAGCGGGTGAGCAGCCTTATTATGGTTTTGATGATAACCAAGT
CAAACTTAACTTCTCGCCAGGTAACTTTATCCAAGTTAACGCTCAGGTTAACCAAGCCATGGTCAGTCAGGCGGTTGAGT
GGCTATCACCAAAGGCGGACGAACGGGTATTAGATCTGTTCTGCGGTATTGGCAACTTTAGTTTGCCATTGGCGAAAGAT
GGCGCCGAGGTGATTGGGGTTGAAGGTGTGCAGGCTATGGTTGAGCAGGCAAGAATTAATGCTAAGCAGAGCGGCTTAGA
TAAAGTCTCTTTCTATCATGCCGATCTGAGCGCCGATCTGTCGAAGGAACCCTGGCTTGGTAAAGTTGATAAGATGCTTA
TCGATCCGGCTCGCGCGGGGGCATACGAAAGCATGCAATCTCTGAAGAAACTAAAGCCGAAAGCCTTAGTCTATGTTTCT
TGTAATCCAGCGAGCCTTGCCCGTGATAGCGAGGTGATTTTAAAGCAAGGTTATCAGCTTAAAAAGATCGGATTAGTGGA
TATGTTTCCACAAACCCATCACTTGGAGTCAATGGCACTGTTTGAGTTAAAAAATTAA

Upstream 100 bases:

>100_bases
ATTCTTTGTATGGGTTACCATTAAATATAGTGTGAGTAATCGATAGACTGCCAGATATTAACCTGTGTTGGCCAGTATTT
TTATCTATTTAGAGAGCAAA

Downstream 100 bases:

>100_bases
ACAAAAAGACACAATTAGATACATTTAGTGAAACCTTTTGCTTAAATATAACTCATACAAATCAAAGTCTGGCACTATAG
GTGTTTTTAGTAGCAATTGT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAQFFKAKPNTSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPGETVSVQLTEQKKKFARAKLLKIDKASAQRVS
PLCPHFDKCGGCDLQHLDIDAQRKHKASTLVDLVNKFAQTQAGEVCDTLSDNAWHYRRRARLATWFDKNTKHISLGFRAS
SSSDVVEIQSCAVLAEPLSALIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKALSDKDKARLVEFANKHQVIVL
LQDDDAQCEHLNGAGEQPYYGFDDNQVKLNFSPGNFIQVNAQVNQAMVSQAVEWLSPKADERVLDLFCGIGNFSLPLAKD
GAEVIGVEGVQAMVEQARINAKQSGLDKVSFYHADLSADLSKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVS
CNPASLARDSEVILKQGYQLKKIGLVDMFPQTHHLESMALFELKN

Sequences:

>Translated_445_residues
MAQFFKAKPNTSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPGETVSVQLTEQKKKFARAKLLKIDKASAQRVS
PLCPHFDKCGGCDLQHLDIDAQRKHKASTLVDLVNKFAQTQAGEVCDTLSDNAWHYRRRARLATWFDKNTKHISLGFRAS
SSSDVVEIQSCAVLAEPLSALIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKALSDKDKARLVEFANKHQVIVL
LQDDDAQCEHLNGAGEQPYYGFDDNQVKLNFSPGNFIQVNAQVNQAMVSQAVEWLSPKADERVLDLFCGIGNFSLPLAKD
GAEVIGVEGVQAMVEQARINAKQSGLDKVSFYHADLSADLSKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVS
CNPASLARDSEVILKQGYQLKKIGLVDMFPQTHHLESMALFELKN
>Mature_444_residues
AQFFKAKPNTSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPGETVSVQLTEQKKKFARAKLLKIDKASAQRVSP
LCPHFDKCGGCDLQHLDIDAQRKHKASTLVDLVNKFAQTQAGEVCDTLSDNAWHYRRRARLATWFDKNTKHISLGFRASS
SSDVVEIQSCAVLAEPLSALIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKALSDKDKARLVEFANKHQVIVLL
QDDDAQCEHLNGAGEQPYYGFDDNQVKLNFSPGNFIQVNAQVNQAMVSQAVEWLSPKADERVLDLFCGIGNFSLPLAKDG
AEVIGVEGVQAMVEQARINAKQSGLDKVSFYHADLSADLSKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVSC
NPASLARDSEVILKQGYQLKKIGLVDMFPQTHHLESMALFELKN

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=195, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI51173878, Length=195, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI269784678, Length=206, Percent_Identity=28.1553398058252, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI269784680, Length=206, Percent_Identity=28.1553398058252, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI269784676, Length=206, Percent_Identity=28.1553398058252, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI20149688, Length=206, Percent_Identity=28.1553398058252, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789148, Length=442, Percent_Identity=46.1538461538462, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787083, Length=382, Percent_Identity=25.3926701570681, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790403, Length=347, Percent_Identity=26.2247838616715, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24666579, Length=195, Percent_Identity=30.2564102564103, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_SHEHH (B0TK00)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673446.1
- ProteinModelPortal:   B0TK00
- SMR:   B0TK00
- GeneID:   5906490
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1218
- HOGENOM:   HBG690163
- OMA:   APYRAFK
- ProtClustDB:   PRK13168
- BioCyc:   SHAL458817:SHAL_1218-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 48990; Mature: 48858

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 401-401 BINDING 278-278 BINDING 307-307 BINDING 312-312 BINDING 328-328 BINDING 355-355 BINDING 375-375

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNTSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPGETVSVQLTEQK
CCCCCCCCCCCHHHHHHHHEEEEEEHHHHCCCEEECCCEEEEEECCCCCCEEEEEEHHHH
KKFARAKLLKIDKASAQRVSPLCPHFDKCGGCDLQHLDIDAQRKHKASTLVDLVNKFAQT
HHHHHHHHHEECCCCHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
QAGEVCDTLSDNAWHYRRRARLATWFDKNTKHISLGFRASSSSDVVEIQSCAVLAEPLSA
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHH
LIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKALSDKDKARLVEFANKHQVIVL
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHCCCCEEEEE
LQDDDAQCEHLNGAGEQPYYGFDDNQVKLNFSPGNFIQVNAQVNQAMVSQAVEWLSPKAD
ECCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCH
ERVLDLFCGIGNFSLPLAKDGAEVIGVEGVQAMVEQARINAKQSGLDKVSFYHADLSADL
HHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCCC
SKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVSCNPASLARDSEVILKQGYQL
CCCCCCCHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCEE
KKIGLVDMFPQTHHLESMALFELKN
EEECEEECCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
AQFFKAKPNTSKQLSSKLSLKVTQLDHLGAGIAHHDGKIVFINGALPGETVSVQLTEQK
CCCCCCCCCCHHHHHHHHEEEEEEHHHHCCCEEECCCEEEEEECCCCCCEEEEEEHHHH
KKFARAKLLKIDKASAQRVSPLCPHFDKCGGCDLQHLDIDAQRKHKASTLVDLVNKFAQT
HHHHHHHHHEECCCCHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
QAGEVCDTLSDNAWHYRRRARLATWFDKNTKHISLGFRASSSSDVVEIQSCAVLAEPLSA
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHH
LIPDLAFMLNQLSGKKALGHVELTQADNGNFVVLRVTKALSDKDKARLVEFANKHQVIVL
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHCCCCEEEEE
LQDDDAQCEHLNGAGEQPYYGFDDNQVKLNFSPGNFIQVNAQVNQAMVSQAVEWLSPKAD
ECCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCH
ERVLDLFCGIGNFSLPLAKDGAEVIGVEGVQAMVEQARINAKQSGLDKVSFYHADLSADL
HHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCCC
SKEPWLGKVDKMLIDPARAGAYESMQSLKKLKPKALVYVSCNPASLARDSEVILKQGYQL
CCCCCCCHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCEE
KKIGLVDMFPQTHHLESMALFELKN
EEECEEECCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA