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The map label for this gene is bepF [H]

Identifier: 167623128

GI number: 167623128

Start: 1446559

End: 1447716

Strand: Direct

Name: bepF [H]

Synonym: Shal_1194

Alternate gene names: 167623128

Gene position: 1446559-1447716 (Clockwise)

Preceding gene: 167623124

Following gene: 167623129

Centisome position: 27.68

GC content: 46.2

Gene sequence:

>1158_bases
ATGGCCGGTTCATCAGTTTCAAAAATTAAGTTGTGGATGTTAGTTTTTAGCGCCACGGCAATGTCAGCCTGTGGTGATGC
GCCAGCACCGCAACAAGCTATGCTGCCAAGCGTTATTGTCAACACAGCTCATACAGAGCTGATCACGCCCAAATCTGAAA
TTGTCGGCCGTACACAGGCATCTGAAGATGTGATTATCAAGTCGCAAATTCATGGCCAGTTGCTCAAACGTACTTTCGTT
GAAGGTGATGATATTACCGCTGGCGATCTATTGTTTGAAATCGATCCATCTACTTATAAAGCTGAGTTAGCACAGCAAAA
TGCAGTATTAAAGCAAGCTGTTGCATCAAGGGATGTGGCGGTGATGAACTGGGAACGTGGACGCCGATTATTGCCTGATG
GCATGATCAGTGCGCAGGATATGGATGAGCTAACTAGCCGTAAACTCACCACTGCCGCAGGAGTCGTACAAGCCGAAGCC
GCCGTGCAAGCTGCAGAGCTGCAATTAAGTTATACCAAGGTATATGCGCCAATATCAGGCCGGATCAGTCACTCAAAGGT
GAGTACCGGTGATATCATTACACCACAATCAGAAATGGCAAGCCTAGTGCAGCTTCAGCCTATGTGGGTAAGCTTTCAAG
TTGCAGAAAAAAAGCTGATTTCAGCCCAACAAAACTTTTCTAAGGCTGTCGAAAAAGACGTTAAAATTACCGATATTGTC
ATGCGCCTTCGTCTACCGAACGGCACCATGTTTGATGAGGTTGGTTATATCGACTTTATTGGTAACCGAGTCGATGCCGC
AACAGGTACCTTGCCTGTTCGCGCTACATTTAAAAATGATGACAAGCTGATGCTTCCAGGCTTGTTTGTCACTCTAGTGA
TTGAATCCCCCATTACTGAAAATGCACTACTTATTCCTCAAGCTGCGGTGCAGGAAGATCAACAAGGCCGTTTCGTGATG
GTGCTTAATGATCAAGATGAAGTACAGAAACGTATCGTCGAACTGGGCGACCGTTTCGGTGTTGAGTGGCGTGTGCTCAG
TGGTCTAGAGGATGGTGAACGCATTGTGGTGGAGGGGTTACAGAAAATTCGTCCAGGTATGAAAGTGAACCCAGTAGAGC
AAGAAGTGGTGCCTTTCAAAGAAGATGAAAAATCATAG

Upstream 100 bases:

>100_bases
TTATGACGCTTTATGGATAAAAGGTTTCATTTGTTGATTGGTTAAGCTTATAATTTCAGAACAAAAATAAAGCACATATC
CTATTTATCAGGAGGCTTAA

Downstream 100 bases:

>100_bases
TCGGAGCAGTCAAGATGAATGAGTTTTTTATTAGAGCTGAAGACCTAAGTTTCCCCGTTTTGGCTAAGCCTTTGTATAAC
GAGGAATGTCATGATGAGTA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MAGSSVSKIKLWMLVFSATAMSACGDAPAPQQAMLPSVIVNTAHTELITPKSEIVGRTQASEDVIIKSQIHGQLLKRTFV
EGDDITAGDLLFEIDPSTYKAELAQQNAVLKQAVASRDVAVMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEA
AVQAAELQLSYTKVYAPISGRISHSKVSTGDIITPQSEMASLVQLQPMWVSFQVAEKKLISAQQNFSKAVEKDVKITDIV
MRLRLPNGTMFDEVGYIDFIGNRVDAATGTLPVRATFKNDDKLMLPGLFVTLVIESPITENALLIPQAAVQEDQQGRFVM
VLNDQDEVQKRIVELGDRFGVEWRVLSGLEDGERIVVEGLQKIRPGMKVNPVEQEVVPFKEDEKS

Sequences:

>Translated_385_residues
MAGSSVSKIKLWMLVFSATAMSACGDAPAPQQAMLPSVIVNTAHTELITPKSEIVGRTQASEDVIIKSQIHGQLLKRTFV
EGDDITAGDLLFEIDPSTYKAELAQQNAVLKQAVASRDVAVMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEA
AVQAAELQLSYTKVYAPISGRISHSKVSTGDIITPQSEMASLVQLQPMWVSFQVAEKKLISAQQNFSKAVEKDVKITDIV
MRLRLPNGTMFDEVGYIDFIGNRVDAATGTLPVRATFKNDDKLMLPGLFVTLVIESPITENALLIPQAAVQEDQQGRFVM
VLNDQDEVQKRIVELGDRFGVEWRVLSGLEDGERIVVEGLQKIRPGMKVNPVEQEVVPFKEDEKS
>Mature_384_residues
AGSSVSKIKLWMLVFSATAMSACGDAPAPQQAMLPSVIVNTAHTELITPKSEIVGRTQASEDVIIKSQIHGQLLKRTFVE
GDDITAGDLLFEIDPSTYKAELAQQNAVLKQAVASRDVAVMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEAA
VQAAELQLSYTKVYAPISGRISHSKVSTGDIITPQSEMASLVQLQPMWVSFQVAEKKLISAQQNFSKAVEKDVKITDIVM
RLRLPNGTMFDEVGYIDFIGNRVDAATGTLPVRATFKNDDKLMLPGLFVTLVIESPITENALLIPQAAVQEDQQGRFVMV
LNDQDEVQKRIVELGDRFGVEWRVLSGLEDGERIVVEGLQKIRPGMKVNPVEQEVVPFKEDEKS

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=362, Percent_Identity=33.1491712707182, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1786668, Length=365, Percent_Identity=33.6986301369863, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1789665, Length=363, Percent_Identity=32.2314049586777, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082036, Length=333, Percent_Identity=30.03003003003, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI87081951, Length=160, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42252; Mature: 42121

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSSVSKIKLWMLVFSATAMSACGDAPAPQQAMLPSVIVNTAHTELITPKSEIVGRTQA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHEEECCHHHHHCCCCC
SEDVIIKSQIHGQLLKRTFVEGDDITAGDLLFEIDPSTYKAELAQQNAVLKQAVASRDVA
CCCEEEHHHHHHHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEE
VMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEAAVQAAELQLSYTKVYAPISG
EEEHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCC
RISHSKVSTGDIITPQSEMASLVQLQPMWVSFQVAEKKLISAQQNFSKAVEKDVKITDIV
CCCCCCCCCCCEECCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHEEEE
MRLRLPNGTMFDEVGYIDFIGNRVDAATGTLPVRATFKNDDKLMLPGLFVTLVIESPITE
EEEECCCCCCHHHCCHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCEEEEEEECCCCCC
NALLIPQAAVQEDQQGRFVMVLNDQDEVQKRIVELGDRFGVEWRVLSGLEDGERIVVEGL
CEEECCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
QKIRPGMKVNPVEQEVVPFKEDEKS
HHHCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
AGSSVSKIKLWMLVFSATAMSACGDAPAPQQAMLPSVIVNTAHTELITPKSEIVGRTQA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHEEECCHHHHHCCCCC
SEDVIIKSQIHGQLLKRTFVEGDDITAGDLLFEIDPSTYKAELAQQNAVLKQAVASRDVA
CCCEEEHHHHHHHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHHHCCEE
VMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEAAVQAAELQLSYTKVYAPISG
EEEHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCC
RISHSKVSTGDIITPQSEMASLVQLQPMWVSFQVAEKKLISAQQNFSKAVEKDVKITDIV
CCCCCCCCCCCEECCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHEEEE
MRLRLPNGTMFDEVGYIDFIGNRVDAATGTLPVRATFKNDDKLMLPGLFVTLVIESPITE
EEEECCCCCCHHHCCHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCEEEEEEECCCCCC
NALLIPQAAVQEDQQGRFVMVLNDQDEVQKRIVELGDRFGVEWRVLSGLEDGERIVVEGL
CEEECCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
QKIRPGMKVNPVEQEVVPFKEDEKS
HHHCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]