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The map label for this gene is purR [H]

Identifier: 167623078

GI number: 167623078

Start: 1387184

End: 1388200

Strand: Reverse

Name: purR [H]

Synonym: Shal_1144

Alternate gene names: 167623078

Gene position: 1388200-1387184 (Counterclockwise)

Preceding gene: 167623081

Following gene: 167623076

Centisome position: 26.56

GC content: 42.67

Gene sequence:

>1017_bases
ATGAAAGTCACCATAAATGATGTCGCCAAACATGCAGGCGTATCGATTAAAACAGTATCACGGGTCACAAATAATGAACC
TTCGGTAAAGCAAGAAACTATCGATAAAGTGAATACTGCTATTAGAGAGCTAAACTATCAGCCTAACTTAGCGGCAAGAA
ATTTGGCTGGGACAAAATCATATGTAATTGGTTTTATATATGATAATCCCAATGCTTACTACATCATCGATATGCAAAAT
GGCATTCTCTCTGCGTGCAAAGATTTGGGTTACGAGTTGCTTATTCACCCGTGTAATTCCCGGGCAGATAACATCTGTGA
AGACCTGACTAAATTAGTTAAGCACAACCGTTTATCTGGTTTAGTGCTAACACCGCCGCTGTCTGAAGATCCGGTGATCT
TAGCGGCACTCGATAAAATCGATGCCAGCTATGTGCGTATTATTGCAGGTGAAAAAGTTGAATCAGGCTCAGGTTTAGCC
GTTCTGGTTAACGACAGATCCGGTGCGATTTCAATTACGCAGCATCTGATTGATCTTGGCCATAAGAATATCGCCTTCTT
GAGCGGTGATCATGAACATGAATCGACCAAAGAGCGTTTAGCTGGGTTTAAGCAGGCATTGAAAGATAACAATATCTCAC
TCAATGAAAGCAATATTATAGATGGGAAGTATTCGTTTGAGTCTGGCGTTCAAGGGGCGAAAATCTTACTTGAGAAACAG
GTGAGGCCGACGGCTATTGTCGCTTGTAATGATGAGATTGCCGCAGGTGCATTATTTGCAGCAAGACTCGAAGGTGTCGA
TATTCCGTCTGATATCTCTATTGTTGGGTTTGAAGATAGCCCATTCTCTCGTCAAACTTGGCCAAAACTCACCACAGTGC
ATCAGCCTAACGAAGAGATCGCGCATATCGCAACTGAATTACTGATAGCTAGGCGCCGAGAAGAAAAGTCAGATCAGACC
AAAGTATTCCTGCCAGAGCCTGTTATTAGAGACTCATCAGCCAAACCAAGAGCTTAA

Upstream 100 bases:

>100_bases
TAAATTTGAGGACAAATTGCACCATCTCAGCGCTGTTTGTTAATCTTGTGTTACCATGATTTGGCAGCACAGAATGTGTA
ACTAACGAGAATCCTGCTTA

Downstream 100 bases:

>100_bases
CGAAAACAGAACTCTACTTCTTCGCATTATTTGCAAAAGCAGATAATGCGAAAAAGTCGCTCAATGCTATACCGCTTCTT
CGTTCTCTTCACCAGTACGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQETIDKVNTAIRELNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACKDLGYELLIHPCNSRADNICEDLTKLVKHNRLSGLVLTPPLSEDPVILAALDKIDASYVRIIAGEKVESGSGLA
VLVNDRSGAISITQHLIDLGHKNIAFLSGDHEHESTKERLAGFKQALKDNNISLNESNIIDGKYSFESGVQGAKILLEKQ
VRPTAIVACNDEIAAGALFAARLEGVDIPSDISIVGFEDSPFSRQTWPKLTTVHQPNEEIAHIATELLIARRREEKSDQT
KVFLPEPVIRDSSAKPRA

Sequences:

>Translated_338_residues
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQETIDKVNTAIRELNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACKDLGYELLIHPCNSRADNICEDLTKLVKHNRLSGLVLTPPLSEDPVILAALDKIDASYVRIIAGEKVESGSGLA
VLVNDRSGAISITQHLIDLGHKNIAFLSGDHEHESTKERLAGFKQALKDNNISLNESNIIDGKYSFESGVQGAKILLEKQ
VRPTAIVACNDEIAAGALFAARLEGVDIPSDISIVGFEDSPFSRQTWPKLTTVHQPNEEIAHIATELLIARRREEKSDQT
KVFLPEPVIRDSSAKPRA
>Mature_338_residues
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQETIDKVNTAIRELNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACKDLGYELLIHPCNSRADNICEDLTKLVKHNRLSGLVLTPPLSEDPVILAALDKIDASYVRIIAGEKVESGSGLA
VLVNDRSGAISITQHLIDLGHKNIAFLSGDHEHESTKERLAGFKQALKDNNISLNESNIIDGKYSFESGVQGAKILLEKQ
VRPTAIVACNDEIAAGALFAARLEGVDIPSDISIVGFEDSPFSRQTWPKLTTVHQPNEEIAHIATELLIARRREEKSDQT
KVFLPEPVIRDSSAKPRA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=305, Percent_Identity=34.0983606557377, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787948, Length=321, Percent_Identity=31.4641744548287, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790194, Length=339, Percent_Identity=30.0884955752212, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=30.0884955752212, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=33.9285714285714, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786540, Length=296, Percent_Identity=32.4324324324324, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790369, Length=306, Percent_Identity=29.4117647058824, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=29.2834890965732, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=27.3885350318471, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789456, Length=332, Percent_Identity=28.3132530120482, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=24.1691842900302, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787906, Length=350, Percent_Identity=25.4285714285714, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786268, Length=321, Percent_Identity=22.7414330218069, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=24.773413897281, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37014; Mature: 37014

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQETIDKVNTAIRELNYQPNLAARNLAGTKS
CEEEHHHHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCE
YVIGFIYDNPNAYYIIDMQNGILSACKDLGYELLIHPCNSRADNICEDLTKLVKHNRLSG
EEEEEEEECCCEEEEEECCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
LVLTPPLSEDPVILAALDKIDASYVRIIAGEKVESGSGLAVLVNDRSGAISITQHLIDLG
EEECCCCCCCCEEEEEEHHCCCCEEEEEECCEECCCCCEEEEEECCCCCHHHHHHHHHCC
HKNIAFLSGDHEHESTKERLAGFKQALKDNNISLNESNIIDGKYSFESGVQGAKILLEKQ
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHCCCCHHHHHHHCC
VRPTAIVACNDEIAAGALFAARLEGVDIPSDISIVGFEDSPFSRQTWPKLTTVHQPNEEI
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHH
AHIATELLIARRREEKSDQTKVFLPEPVIRDSSAKPRA
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVTINDVAKHAGVSIKTVSRVTNNEPSVKQETIDKVNTAIRELNYQPNLAARNLAGTKS
CEEEHHHHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCE
YVIGFIYDNPNAYYIIDMQNGILSACKDLGYELLIHPCNSRADNICEDLTKLVKHNRLSG
EEEEEEEECCCEEEEEECCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
LVLTPPLSEDPVILAALDKIDASYVRIIAGEKVESGSGLAVLVNDRSGAISITQHLIDLG
EEECCCCCCCCEEEEEEHHCCCCEEEEEECCEECCCCCEEEEEECCCCCHHHHHHHHHCC
HKNIAFLSGDHEHESTKERLAGFKQALKDNNISLNESNIIDGKYSFESGVQGAKILLEKQ
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHCCCCHHHHHHHCC
VRPTAIVACNDEIAAGALFAARLEGVDIPSDISIVGFEDSPFSRQTWPKLTTVHQPNEEI
CCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHH
AHIATELLIARRREEKSDQTKVFLPEPVIRDSSAKPRA
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA