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The map label for this gene is trmD

Identifier: 167623033

GI number: 167623033

Start: 1337313

End: 1338059

Strand: Direct

Name: trmD

Synonym: Shal_1099

Alternate gene names: 167623033

Gene position: 1337313-1338059 (Clockwise)

Preceding gene: 167623032

Following gene: 167623034

Centisome position: 25.59

GC content: 47.39

Gene sequence:

>747_bases
ATGTGGTTAGGGGTAGTAACCCTGTTTCCAGAGATGTTTCGTGCCGTAACAGACTTTGGTGTAACGGGTCGTGCAGTTAG
CAAAGGGTTGCTAGAGTTGCAAACGTGGAATCCTCGAGATTTCACCCATGATAAACATAAAACAGTGGATGACCGCCCTT
ATGGTGGTGGCCCTGGAATGTTAATGATGGTGCAGCCTCTACGTGATGCCATCCATGCAGCAAAAGCTGCAGCTGGAAAA
GAGGCGAAGGTGATCTACCTTTCTCCTCAAGGCCGTAAGCTGACACAGCAAGGCGTTGAAGAGTTAGCAAAGTCATCCAG
TTTGGTTTTAGTGTGTGGACGATACGAAGGTGTCGATGAGCGCATTATTCAAACTGAAGTGGACGAAGAGTGGTCGATAG
GAGATTACGTGCTTTCGGGCGGTGAACTTCCAGCGATGACTCTGATCGATTCAGTATCTAGATTGGTTCCTGGCGTGCTA
GGAAAACAAGCTTCCGCAGAGCAGGATTCCTTCTCTGATGGATTACTGGATTGCCCCCACTATACGCGTCCTGAAAGTTT
GGATGGCCTGGATGTTCCAGCCGTATTGTTAAGTGGTAACCACGAACATATTAGACGCTGGCGTCTACAGCAAAGCCTAG
GTAGGACTTTGTTGAGACGACCAGAATTACTTGAAAATCTAGCTCTGACTGGCGAACAAGAAAAGCTTTTAGCCGATTTT
GTTGACACCATCAAACAAGATGATTAG

Upstream 100 bases:

>100_bases
GATTCCCTTTGTTACTGACCAGTTCATCCAAAAGGTGGACTTGGCAGCAAAACAGATTTTAGTGGATTGGGATCCGGACT
TCTAAGTCGAGGTACAACAT

Downstream 100 bases:

>100_bases
TCGAAGCGCAGTTATTACTAGTATGGAGTTTATTTAATGAATAACATCATCAAAATGCTCAACGAAGAGCAAATGAAAAC
CGACGTACCACAATTTGGTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MWLGVVTLFPEMFRAVTDFGVTGRAVSKGLLELQTWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGK
EAKVIYLSPQGRKLTQQGVEELAKSSSLVLVCGRYEGVDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLDVPAVLLSGNHEHIRRWRLQQSLGRTLLRRPELLENLALTGEQEKLLADF
VDTIKQDD

Sequences:

>Translated_248_residues
MWLGVVTLFPEMFRAVTDFGVTGRAVSKGLLELQTWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGK
EAKVIYLSPQGRKLTQQGVEELAKSSSLVLVCGRYEGVDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLDVPAVLLSGNHEHIRRWRLQQSLGRTLLRRPELLENLALTGEQEKLLADF
VDTIKQDD
>Mature_248_residues
MWLGVVTLFPEMFRAVTDFGVTGRAVSKGLLELQTWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGK
EAKVIYLSPQGRKLTQQGVEELAKSSSLVLVCGRYEGVDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLDVPAVLLSGNHEHIRRWRLQQSLGRTLLRRPELLENLALTGEQEKLLADF
VDTIKQDD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=240, Percent_Identity=80, Blast_Score=390, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_SHEHH (B0TJ77)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673327.1
- ProteinModelPortal:   B0TJ77
- SMR:   B0TJ77
- GeneID:   5905179
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1099
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   SHAL458817:SHAL_1099-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27436; Mature: 27436

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLGVVTLFPEMFRAVTDFGVTGRAVSKGLLELQTWNPRDFTHDKHKTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGKEAKVIYLSPQGRKLTQQGVEELAKSSSLVLVCGRYEGVDE
EEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHH
RIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGLDVPAVLLSGNHEHIRRWRLQQSLGRTLLRRPELLENLALTGEQEKLLADF
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
VDTIKQDD
HHHHCCCC
>Mature Secondary Structure
MWLGVVTLFPEMFRAVTDFGVTGRAVSKGLLELQTWNPRDFTHDKHKTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGKEAKVIYLSPQGRKLTQQGVEELAKSSSLVLVCGRYEGVDE
EEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHH
RIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGLDVPAVLLSGNHEHIRRWRLQQSLGRTLLRRPELLENLALTGEQEKLLADF
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
VDTIKQDD
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA