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The map label for this gene is thyA

Identifier: 167623004

GI number: 167623004

Start: 1309508

End: 1310359

Strand: Direct

Name: thyA

Synonym: Shal_1070

Alternate gene names: 167623004

Gene position: 1309508-1310359 (Clockwise)

Preceding gene: 167623003

Following gene: 167623005

Centisome position: 25.05

GC content: 42.37

Gene sequence:

>852_bases
ATGAAACAGTATTTAGATCTTTGTCAGCGTATTATTGATGAAGGGACTTGGGTTGAGAATAGCCGCACCGGTAAACGTTG
TCTTACCGTTATTAATGCTGATCTGGTTTATAACGTCGATAAAAATGAATTCCCACTTATCACTACTCGGAAAAGCTTTT
ATAAGGCTGCAATCGCTGAGCTGCTGGGTTATATCCGAGGTTATGATAATGCCGCTGATTTTAGAAAAATAGGCTGTAAT
ACTTGGAACGCTAATGCTAACGACAATCAAGCTTGGTTGAATAATCCACACCGTAAAGGTGTTGATGACATGGGCCGAGT
CTATGGTGTACAGGGCCGTGCATGGAGCAAACCAGATGGCGGCACTATCGACCAGTTACGTAAAATTGTAGATGACCTAT
CTAAGGGAATTGATGATAGAGGTGAGATCTTGAGCTTCTATAACCCGGGTGAATTTCATATGGGGTGTTTAAGGCCTTGT
ATGCACACTCATAACTTCTCATTGCTGGGTGATACTTTACACCTTACTAGCTTTCAACGCTCATGTGATGTGCCTTTAGG
ACTGAACTTCAACCAGGTTCAGGTATTTACGCTATTGGCGTTAATGGCACAAATTACTGGCCATAAAGCGGGAACCGCAT
TCCATAAAATTGTTAACGCGCATATCTATGAAGATCAGTTAGAACTGATGCGAGATGTACAGATTAAGCGCGAGCCGCTC
TCCTCGCCGAAGCTAGTGATTAACCCTGATATCAAGTCACTAGAAGATCTTGAGACTTGGGTGACTATGGATGACTTTGA
AGTAACAGGTTATGAACACCACGAGGCGATTAAATATCCATTCTCAGTGTAA

Upstream 100 bases:

>100_bases
ACTTGTTTTAGCATAATGTTCTACTGAGCAATTATTGGTAGTATTTTCTCGTTTAGCGCTGAGAGTCAGCTCAGCAACTC
TTTTTTATAGGTAGATAATC

Downstream 100 bases:

>100_bases
CTTTTCCTGATTACAAATCAGGCTTTAAAAACTCGCCAAATGGCGAGTTTTTTTATCCAATAATGGCCAAACTCTCGAGT
AATACCAATCAGTATAAGAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLDLCQRIIDEGTWVENSRTGKRCLTVINADLVYNVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRKIGCN
TWNANANDNQAWLNNPHRKGVDDMGRVYGVQGRAWSKPDGGTIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPC
MHTHNFSLLGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKAGTAFHKIVNAHIYEDQLELMRDVQIKREPL
SSPKLVINPDIKSLEDLETWVTMDDFEVTGYEHHEAIKYPFSV

Sequences:

>Translated_283_residues
MKQYLDLCQRIIDEGTWVENSRTGKRCLTVINADLVYNVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRKIGCN
TWNANANDNQAWLNNPHRKGVDDMGRVYGVQGRAWSKPDGGTIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPC
MHTHNFSLLGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKAGTAFHKIVNAHIYEDQLELMRDVQIKREPL
SSPKLVINPDIKSLEDLETWVTMDDFEVTGYEHHEAIKYPFSV
>Mature_283_residues
MKQYLDLCQRIIDEGTWVENSRTGKRCLTVINADLVYNVDKNEFPLITTRKSFYKAAIAELLGYIRGYDNAADFRKIGCN
TWNANANDNQAWLNNPHRKGVDDMGRVYGVQGRAWSKPDGGTIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPC
MHTHNFSLLGDTLHLTSFQRSCDVPLGLNFNQVQVFTLLALMAQITGHKAGTAFHKIVNAHIYEDQLELMRDVQIKREPL
SSPKLVINPDIKSLEDLETWVTMDDFEVTGYEHHEAIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=32.1799307958478, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=34.2756183745583, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71993377, Length=276, Percent_Identity=27.536231884058, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324648, Length=302, Percent_Identity=27.8145695364238, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17137556, Length=290, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_SHEHH (B0TIU4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673298.1
- ProteinModelPortal:   B0TIU4
- SMR:   B0TIU4
- GeneID:   5903314
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1070
- HOGENOM:   HBG588098
- OMA:   GRAWRKP
- ProtClustDB:   PRK01827
- BioCyc:   SHAL458817:SHAL_1070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32257; Mature: 32257

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLCQRIIDEGTWVENSRTGKRCLTVINADLVYNVDKNEFPLITTRKSFYKAAIAE
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEEEEEECCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRKIGCNTWNANANDNQAWLNNPHRKGVDDMGRVYGVQGRAWSKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHEECCCCCCCCCCC
GTIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPCMHTHNFSLLGDTLHLTSFQR
CCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHCCCHHHHHHHHH
SCDVPLGLNFNQVQVFTLLALMAQITGHKAGTAFHKIVNAHIYEDQLELMRDVQIKREPL
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SSPKLVINPDIKSLEDLETWVTMDDFEVTGYEHHEAIKYPFSV
CCCCEEECCCHHHHHHHHHHEEECCEEECCCCCCHHCCCCCCC
>Mature Secondary Structure
MKQYLDLCQRIIDEGTWVENSRTGKRCLTVINADLVYNVDKNEFPLITTRKSFYKAAIAE
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEEEEEECCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRKIGCNTWNANANDNQAWLNNPHRKGVDDMGRVYGVQGRAWSKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHEECCCCCCCCCCC
GTIDQLRKIVDDLSKGIDDRGEILSFYNPGEFHMGCLRPCMHTHNFSLLGDTLHLTSFQR
CCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHCCCHHHHHHHHH
SCDVPLGLNFNQVQVFTLLALMAQITGHKAGTAFHKIVNAHIYEDQLELMRDVQIKREPL
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SSPKLVINPDIKSLEDLETWVTMDDFEVTGYEHHEAIKYPFSV
CCCCEEECCCHHHHHHHHHHEEECCEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA