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The map label for this gene is gltD [H]

Identifier: 167622994

GI number: 167622994

Start: 1294179

End: 1295588

Strand: Reverse

Name: gltD [H]

Synonym: Shal_1060

Alternate gene names: 167622994

Gene position: 1295588-1294179 (Counterclockwise)

Preceding gene: 167622995

Following gene: 167622993

Centisome position: 24.79

GC content: 51.42

Gene sequence:

>1410_bases
ATGAGCAATGATTTTCAGTTTCTTGAAGTGGGCCGCAAAGACCCAGCTAAACACGCCGCCAAACAACGTGCAACGCAGTT
TATTGAGATTTATCAACCTTTTGCCCAACCTCAAGTTAAAGAGCAGGCCGACCGTTGTTTAGACTGTGGTAACCCATATT
GTGAATGGAAATGTCCACTGCATAACTACATCCCTAACTGGCTTCAATTAGCTAAACAGGGGCGTATTATGGAGGCAGCG
GATTTAGTCCACGAGACTAATACCCTACCGGAGATCTGTGGCCGTGTTTGCCCACAAGATAGGCTCTGTGAAGGGGCCTG
TACCCTTAACGAAGAGTTTGGCGCAGTCACCATCGGTAATGTGGAAAAGTATATTACCGACACAGCTATCTCACAGGGCT
GGCGCCCTGATCTGTCAATGGTCACACCGCGTAAAGAGCGCGTGGCTATTGTGGGTGCGGGGCCAGCTGGTCTTGGCTGC
GCCGATATCTTGGCCCGTAACGGCGTAAAAGCGGTGGTGTTTGATAAAAACGTGCAAATTGGTGGTCTACTGACCTACGG
TATTCCCTCATTCAAGCTTGATAAAGAGGTGATGCAGGTTCGCCGCACCGTGCTTGAAGGAATGGGCATCGAATTTAAGC
TTGGCGTTACCGTTGGCAAGGATATCGACTTTAAAGAGTTAATCGAGCACTACGATGCCGTCTTTCTCGGTATGGGTACC
TATACCGCCATGAAGGCAAACCTGCCTGGCGAAGATGCTAATGGGGTGTATCAGGCGCTACCTTACTTGATTGGTAACAC
CCATAACGTTATGGGCACCGAGTGTATTGAGACCCCCTATCTCAGCCTTAAGGGCAAACGCGTCGTCGTGCTAGGTGGTG
GTGACACCGCAATGGACTGTGTCCGTACTGCGGTGCGTCAGGGAGCGACTCAAGTCATCTGTGCCTATCGCCGCGATGAA
CAGAATATGCCGGGCTCTCGCCGTGAAGTGCAAAATGCTCGCGAGGAAGGCGTTGAGTTTCTATTTAATCGTCAACCAAC
GGCAATCAAAACTGTCGATGGCAAGGTGGTGGGCATCGAGTGCATCGAGACCCAGCTCGGTGAAGCCGATGAAAGTGGAC
GTCGCCGCCCAGAGCCAATAACGGGGAGTGAGCAAGTGCTCGATGCCGATGCCGTCATTATTGCCTTTGGTTTCCAACCT
AGCCCAGCAACGTGGCTAGGCGACTACAATATCGAGATCAATGATTGGGGCTTGGTCAAGGCGCCAAAAGTCGCCGATAA
TCCATTCCAAACCAGCAACCCGAAGGTGTTTGCTGGCGGCGATATGGTGCGAGGTTCAGACCTGGTCGTTACCGCTATTG
CAGAGGGGCGCGATGCGGCTATGGGAATTTTAAATTACTTAGAAGACTAA

Upstream 100 bases:

>100_bases
TTGCTTTATTTTAGTGAAACCCAAAAGCGTTGAACTAGACGATCTATTAAAGTTAGAGCAATCAGAACCTGCACTAGCAG
TCAGAGCGGGGTAAGTAAAA

Downstream 100 bases:

>100_bases
TATTTTAAAAGAGGTTTATAGAGGATGTTTAAAAGAGGTTTATAGAAGGCTGACTTGAATGAAAGCTTACTTATAAACCT
CTAGCCTAAAACATTGGCGA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSNDFQFLEVGRKDPAKHAAKQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPLHNYIPNWLQLAKQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGNVEKYITDTAISQGWRPDLSMVTPRKERVAIVGAGPAGLGC
ADILARNGVKAVVFDKNVQIGGLLTYGIPSFKLDKEVMQVRRTVLEGMGIEFKLGVTVGKDIDFKELIEHYDAVFLGMGT
YTAMKANLPGEDANGVYQALPYLIGNTHNVMGTECIETPYLSLKGKRVVVLGGGDTAMDCVRTAVRQGATQVICAYRRDE
QNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIECIETQLGEADESGRRRPEPITGSEQVLDADAVIIAFGFQP
SPATWLGDYNIEINDWGLVKAPKVADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED

Sequences:

>Translated_469_residues
MSNDFQFLEVGRKDPAKHAAKQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPLHNYIPNWLQLAKQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGNVEKYITDTAISQGWRPDLSMVTPRKERVAIVGAGPAGLGC
ADILARNGVKAVVFDKNVQIGGLLTYGIPSFKLDKEVMQVRRTVLEGMGIEFKLGVTVGKDIDFKELIEHYDAVFLGMGT
YTAMKANLPGEDANGVYQALPYLIGNTHNVMGTECIETPYLSLKGKRVVVLGGGDTAMDCVRTAVRQGATQVICAYRRDE
QNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIECIETQLGEADESGRRRPEPITGSEQVLDADAVIIAFGFQP
SPATWLGDYNIEINDWGLVKAPKVADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED
>Mature_468_residues
SNDFQFLEVGRKDPAKHAAKQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPLHNYIPNWLQLAKQGRIMEAAD
LVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGNVEKYITDTAISQGWRPDLSMVTPRKERVAIVGAGPAGLGCA
DILARNGVKAVVFDKNVQIGGLLTYGIPSFKLDKEVMQVRRTVLEGMGIEFKLGVTVGKDIDFKELIEHYDAVFLGMGTY
TAMKANLPGEDANGVYQALPYLIGNTHNVMGTECIETPYLSLKGKRVVVLGGGDTAMDCVRTAVRQGATQVICAYRRDEQ
NMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIECIETQLGEADESGRRRPEPITGSEQVLDADAVIIAFGFQPS
PATWLGDYNIEINDWGLVKAPKVADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=455, Percent_Identity=30.5494505494506, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=64.7435897435898, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=460, Percent_Identity=59.3478260869565, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI87082180, Length=460, Percent_Identity=55.8695652173913, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1788468, Length=443, Percent_Identity=28.2167042889391, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789243, Length=426, Percent_Identity=31.6901408450704, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17570289, Length=492, Percent_Identity=32.9268292682927, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI71984108, Length=447, Percent_Identity=31.7673378076063, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17543860, Length=212, Percent_Identity=30.6603773584906, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=482, Percent_Identity=31.5352697095436, Blast_Score=229, Evalue=9e-61,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=36.3636363636364, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=36.3636363636364, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=36.3636363636364, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=36.3636363636364, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24640763, Length=476, Percent_Identity=28.781512605042, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI18858217, Length=476, Percent_Identity=28.781512605042, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51430; Mature: 51299

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFQFLEVGRKDPAKHAAKQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPL
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLQLAKQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECC
VEKYITDTAISQGWRPDLSMVTPRKERVAIVGAGPAGLGCADILARNGVKAVVFDKNVQI
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCEE
GGLLTYGIPSFKLDKEVMQVRRTVLEGMGIEFKLGVTVGKDIDFKELIEHYDAVFLGMGT
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHEEEEECCC
YTAMKANLPGEDANGVYQALPYLIGNTHNVMGTECIETPYLSLKGKRVVVLGGGDTAMDC
CEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCEEECCCEEEEECCCCHHHHH
VRTAVRQGATQVICAYRRDEQNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIE
HHHHHHCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEE
CIETQLGEADESGRRRPEPITGSEQVLDADAVIIAFGFQPSPATWLGDYNIEINDWGLVK
EHHHHCCCCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCEEECEEEEECCCCCEE
APKVADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED
CCCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SNDFQFLEVGRKDPAKHAAKQRATQFIEIYQPFAQPQVKEQADRCLDCGNPYCEWKCPL
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLQLAKQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNEEFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECC
VEKYITDTAISQGWRPDLSMVTPRKERVAIVGAGPAGLGCADILARNGVKAVVFDKNVQI
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCEE
GGLLTYGIPSFKLDKEVMQVRRTVLEGMGIEFKLGVTVGKDIDFKELIEHYDAVFLGMGT
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHEEEEECCC
YTAMKANLPGEDANGVYQALPYLIGNTHNVMGTECIETPYLSLKGKRVVVLGGGDTAMDC
CEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCEEECCCEEEEECCCCHHHHH
VRTAVRQGATQVICAYRRDEQNMPGSRREVQNAREEGVEFLFNRQPTAIKTVDGKVVGIE
HHHHHHCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEE
CIETQLGEADESGRRRPEPITGSEQVLDADAVIIAFGFQPSPATWLGDYNIEINDWGLVK
EHHHHCCCCCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCEEECEEEEECCCCCEE
APKVADNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGRDAAMGILNYLED
CCCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]