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The map label for this gene is phoB [H]

Identifier: 167622973

GI number: 167622973

Start: 1266841

End: 1268253

Strand: Reverse

Name: phoB [H]

Synonym: Shal_1039

Alternate gene names: 167622973

Gene position: 1268253-1266841 (Counterclockwise)

Preceding gene: 167622974

Following gene: 167622972

Centisome position: 24.26

GC content: 48.27

Gene sequence:

>1413_bases
ATGACTAATTTAAACTCGATTGTTCGTTTATCGGCAATTTACTCAAGCCTTTTGTGTGCCAGTCTGTGGTGCACTTCGGC
TCAAGCACAACCCGATGCCCCCTTTGCTCCAGTAATTGTAGCCGAATACCTTCCTAGAAATATCATCTACTTAATTGGTG
ACGGTATGGGACCGGCTTATACCTCTGCCTACCGTTTCTATAACGACAACCCGCAAACCCAAAGAGTCGAAAAGACTATC
TTCGACCAACTGTTAGTCGGTATGTCGAGCACCTACCCCGACGATGACACCTATGTCACCGACTCGGCGGCCGCAGCTAC
GGCGCTCGCGACCAGCTACAAAACCTACAACGGTGCGATTTCGGTTGACCATCAAGGCGGGCCCTTCCCCACATTACTGG
AAATGGCAAAAGCTCAAGGAAAAACCACCGCAGTGGTCGTTACCTCACAGATTAACCATGCTACCCCCGCCAGCTTTTTA
GCCCACAATGAAAGTCGCAGGAACTATGACCAAATAGCCGATAGTTATCTGTCTAACCTTATCGACAAGCGCCCGGTTGC
CGACCTCATGCTTGGCGGTGGCACACAATATTTTGCTCGCAACGACCGAGATCTAACATTAGAGTTTCAAGAGTATGGCT
ATCACTATATTGATAGCCTTAGCGATCTAAGCAGTATCGAAAATCTGCCCGCTCTAGGGCTGTTTGCCCCTAAAGGTTTA
CCCTATGCGATTGAAAGCCAAGACCCTCTTAGACTGACTACCATGACCCGCAAGGCTCTCTCCTTGCTATCGAATCAAGA
AGCCCCCTTTGTGATGATGGTAGAAGCCAGTCATATAGATTGGTGTGGCCACAGCAACGATATCGCGTGCGCGATGAATG
AGATGGATGACTTCGCTAATACCTTAAGTCTAGTTAAGCGCTATGTCGATAGTCACCCTGACACCTTACTCGTGGCGACG
GCGGACCACTCTACCGGTGGCCTGACATTGGGCAGGGACGGTACTTATCAATGGCAAGGCCAGTTACTACATCAAGTTCA
CAGCTTGCCAAGCACGATTTCCCAGCTCATCATTGCCAACCCAGAGGTGCTAAATGATTTAGCCGCATTTAGTGCTTTTT
TACGCCCCCATATCAGCATTGATATTGAAGACAGAAAAATGGATGGGCTAAGAAAAATGCTCAACTCTCGTTTGTCTCAC
CACAAGCATCAACAGAAAATAGAGGACGATCTAAAACAAACGATTGATAAGCTGACCTATACAGGATGGACAACGGGTGG
CCATGACGCAATCGATGTGCCTGTTTACGCCTATGGCCAAGGCGCGCTGTACTTTAGAGGTCATAAAGACAATAGCGAAA
TCGCTGCGACGCTTATTCAGATGGTGCAAGATGAGCGCTACAAGGTTAAGTAA

Upstream 100 bases:

>100_bases
CAAATGACTTTTCATTAAACCTTAACCCCGATATAAGGCTGCACGAGCGCAATGAGTTGCAGCCATTTATTTCCCATTTG
CGATTTTGGATAGCTGACCC

Downstream 100 bases:

>100_bases
CCGTTATATGAACCTGACTCAACAATGACTGCCGAGTTTATTGGCATACTTTGGCTTGTCTTGGGTGGCTGTAAGATTAG
CTGTCAATATAGAGAAACAG

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTNLNSIVRLSAIYSSLLCASLWCTSAQAQPDAPFAPVIVAEYLPRNIIYLIGDGMGPAYTSAYRFYNDNPQTQRVEKTI
FDQLLVGMSSTYPDDDTYVTDSAAAATALATSYKTYNGAISVDHQGGPFPTLLEMAKAQGKTTAVVVTSQINHATPASFL
AHNESRRNYDQIADSYLSNLIDKRPVADLMLGGGTQYFARNDRDLTLEFQEYGYHYIDSLSDLSSIENLPALGLFAPKGL
PYAIESQDPLRLTTMTRKALSLLSNQEAPFVMMVEASHIDWCGHSNDIACAMNEMDDFANTLSLVKRYVDSHPDTLLVAT
ADHSTGGLTLGRDGTYQWQGQLLHQVHSLPSTISQLIIANPEVLNDLAAFSAFLRPHISIDIEDRKMDGLRKMLNSRLSH
HKHQQKIEDDLKQTIDKLTYTGWTTGGHDAIDVPVYAYGQGALYFRGHKDNSEIAATLIQMVQDERYKVK

Sequences:

>Translated_470_residues
MTNLNSIVRLSAIYSSLLCASLWCTSAQAQPDAPFAPVIVAEYLPRNIIYLIGDGMGPAYTSAYRFYNDNPQTQRVEKTI
FDQLLVGMSSTYPDDDTYVTDSAAAATALATSYKTYNGAISVDHQGGPFPTLLEMAKAQGKTTAVVVTSQINHATPASFL
AHNESRRNYDQIADSYLSNLIDKRPVADLMLGGGTQYFARNDRDLTLEFQEYGYHYIDSLSDLSSIENLPALGLFAPKGL
PYAIESQDPLRLTTMTRKALSLLSNQEAPFVMMVEASHIDWCGHSNDIACAMNEMDDFANTLSLVKRYVDSHPDTLLVAT
ADHSTGGLTLGRDGTYQWQGQLLHQVHSLPSTISQLIIANPEVLNDLAAFSAFLRPHISIDIEDRKMDGLRKMLNSRLSH
HKHQQKIEDDLKQTIDKLTYTGWTTGGHDAIDVPVYAYGQGALYFRGHKDNSEIAATLIQMVQDERYKVK
>Mature_469_residues
TNLNSIVRLSAIYSSLLCASLWCTSAQAQPDAPFAPVIVAEYLPRNIIYLIGDGMGPAYTSAYRFYNDNPQTQRVEKTIF
DQLLVGMSSTYPDDDTYVTDSAAAATALATSYKTYNGAISVDHQGGPFPTLLEMAKAQGKTTAVVVTSQINHATPASFLA
HNESRRNYDQIADSYLSNLIDKRPVADLMLGGGTQYFARNDRDLTLEFQEYGYHYIDSLSDLSSIENLPALGLFAPKGLP
YAIESQDPLRLTTMTRKALSLLSNQEAPFVMMVEASHIDWCGHSNDIACAMNEMDDFANTLSLVKRYVDSHPDTLLVATA
DHSTGGLTLGRDGTYQWQGQLLHQVHSLPSTISQLIIANPEVLNDLAAFSAFLRPHISIDIEDRKMDGLRKMLNSRLSHH
KHQQKIEDDLKQTIDKLTYTGWTTGGHDAIDVPVYAYGQGALYFRGHKDNSEIAATLIQMVQDERYKVK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=340, Percent_Identity=27.3529411764706, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI294660770, Length=333, Percent_Identity=27.027027027027, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI94721246, Length=338, Percent_Identity=26.9230769230769, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI157266296, Length=338, Percent_Identity=27.5147928994083, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157266292, Length=346, Percent_Identity=27.4566473988439, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI294660772, Length=280, Percent_Identity=26.4285714285714, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI48994877, Length=336, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=504, Percent_Identity=32.5396825396825, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI18859923, Length=327, Percent_Identity=28.7461773700306, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24651554, Length=341, Percent_Identity=29.9120234604106, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355149, Length=340, Percent_Identity=29.1176470588235, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21355981, Length=324, Percent_Identity=28.3950617283951, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355151, Length=326, Percent_Identity=26.6871165644172, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85725200, Length=333, Percent_Identity=27.9279279279279, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI85815833, Length=333, Percent_Identity=27.9279279279279, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921912, Length=359, Percent_Identity=26.7409470752089, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358067, Length=335, Percent_Identity=26.5671641791045, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 52065; Mature: 51934

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNSIVRLSAIYSSLLCASLWCTSAQAQPDAPFAPVIVAEYLPRNIIYLIGDGMGPAY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHH
TSAYRFYNDNPQTQRVEKTIFDQLLVGMSSTYPDDDTYVTDSAAAATALATSYKTYNGAI
HHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHEECCCEE
SVDHQGGPFPTLLEMAKAQGKTTAVVVTSQINHATPASFLAHNESRRNYDQIADSYLSNL
EECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHH
IDKRPVADLMLGGGTQYFARNDRDLTLEFQEYGYHYIDSLSDLSSIENLPALGLFAPKGL
HHCCCHHHHHCCCCCCEEECCCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCC
PYAIESQDPLRLTTMTRKALSLLSNQEAPFVMMVEASHIDWCGHSNDIACAMNEMDDFAN
CEEECCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCEEEEHHHHHHHHH
TLSLVKRYVDSHPDTLLVATADHSTGGLTLGRDGTYQWQGQLLHQVHSLPSTISQLIIAN
HHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PEVLNDLAAFSAFLRPHISIDIEDRKMDGLRKMLNSRLSHHKHQQKIEDDLKQTIDKLTY
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TGWTTGGHDAIDVPVYAYGQGALYFRGHKDNSEIAATLIQMVQDERYKVK
CCCCCCCCCCEECCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TNLNSIVRLSAIYSSLLCASLWCTSAQAQPDAPFAPVIVAEYLPRNIIYLIGDGMGPAY
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHH
TSAYRFYNDNPQTQRVEKTIFDQLLVGMSSTYPDDDTYVTDSAAAATALATSYKTYNGAI
HHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHEECCCEE
SVDHQGGPFPTLLEMAKAQGKTTAVVVTSQINHATPASFLAHNESRRNYDQIADSYLSNL
EECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHH
IDKRPVADLMLGGGTQYFARNDRDLTLEFQEYGYHYIDSLSDLSSIENLPALGLFAPKGL
HHCCCHHHHHCCCCCCEEECCCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCC
PYAIESQDPLRLTTMTRKALSLLSNQEAPFVMMVEASHIDWCGHSNDIACAMNEMDDFAN
CEEECCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCEEEEHHHHHHHHH
TLSLVKRYVDSHPDTLLVATADHSTGGLTLGRDGTYQWQGQLLHQVHSLPSTISQLIIAN
HHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PEVLNDLAAFSAFLRPHISIDIEDRKMDGLRKMLNSRLSHHKHQQKIEDDLKQTIDKLTY
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TGWTTGGHDAIDVPVYAYGQGALYFRGHKDNSEIAATLIQMVQDERYKVK
CCCCCCCCCCEECCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]