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The map label for this gene is dnaG [H]

Identifier: 167622944

GI number: 167622944

Start: 1236663

End: 1238393

Strand: Reverse

Name: dnaG [H]

Synonym: Shal_1005

Alternate gene names: 167622944

Gene position: 1238393-1236663 (Counterclockwise)

Preceding gene: 167622945

Following gene: 167622943

Centisome position: 23.69

GC content: 44.71

Gene sequence:

>1731_bases
ATGGCGATACCTCGTGATTTTATAAATGAGCTAGTAGCTCGCACTGATATTGTCGATCTCATCGACCCTAAAGTGCCCCT
AAAAAAGGCGGGTAAAAACCACTCTGCCTGTTGTCCTTTTCATAGCGAAAAATCTCCCTCTTTTACTGTCAGCAGAGACA
AACAGTTTTATCATTGTTTTGGCTGCGGAGCACACGGCAACGCTATCGATTTTGTCATGGAATATGACCGACTCGACTTT
GTTGATGCTATTGAAGAACTCGCCGGACAACTTGGACTCGAAGTCCCAAGGGAACAAGGCACTGGCAAACCACGCGATCA
AGAGCTGAGTCGCGACTTATATCAGTTGATGGAAGAGGCTAGCCTGTTCTTTCAAAGCCAACTTAGGCAACATACAGACA
AACAAAAAGTCCTTGATTATCTTAAACATCGAGGCCTTTCAAAAGAAGTGGTTGAACACTTCAATATCGGCTTTGCACCC
GATGGCTGGGATGGTCTACTAAGCCGTTACCGCCAAAACCAAGACTCGCAAGATAAACTGCTTACCGCAGGCATGGTCAT
CGAGAATGACAGTGGCAAACGCTACGATAGATTCCGCGACCGTTTAATGTTCCCAATCAGGGACCGACGCGGACGAGTGA
TCGGCTTTGGCGGCCGAGTGTTAGGTGACGGTACACCGAAGTACTTGAATTCGCCAGAAACGCCCATATTTCATAAAGGC
AATGAGCTTTATGGTCTATACGAATTAAAACAGCGTCACCGCGAACCGCAAAGAGTCTTGATTGTTGAAGGCTATATGGA
TGTTGTGGCACTGGCACAGTTTGACGTTGACTACGCTGTCGCCTCACTCGGTACCTCGACAACGGCTGAACAATTTCAGT
TATTACTACGTAGTGCAAAAGAGGTCATCTGCTGTTATGACGGTGACAAAGCGGGTAAAGAAGCCGCATGGCGAGCGTTA
GAAACAGCATTGCCACTACTCAAACCCGGTAATCAGGTTCGATTTATGTTCCTTCCTGAGGGGGAAGATCCCGACACCTT
GGTTAGACAAATTGGCAAACAAGAGTTTGAAGCGCAAATCGACCAAGCTATGGCGCTACCTGAGTTTTTGTTTGAGAACC
TTATAAAAACTTACGGCAATGAAGATAAGAGTGTTTTGGCAAAAAATGCCATTAAACTCATAGAAAAAGTACAGGACACC
GTATTACAGAGCCTGTTACTCGAAGATTTAGCACATAAGCTAAGAATGAACGGTGCCGATGAACTTAAGCGAAAATTTAA
CTTTTCGACTAAGGCGGCCGAAAAGCCAAATCAGCACAAAGCGTTGAAAGGACGTGGTACGCCTCTGCGATTAGCCATTG
CATTGCTAGTACAACATCCGCATTTGGGTGAAAAACTACCCGAACAACCAGCGCTAAAACATATACAGATGGCTGGCATT
GATTTGCTAACCCTTGTATTAGATTTAACTCGCGGGCAAGTGATAACCAGCGCACAACTACTTGAACAGTTCAGGGGCGA
TAACCAATTCAGCACCCTAAAAAAACTGACCCAATGGGATCATCAAGTGGCGGATGAAAACTTGCTACAAAAGTTAAAAG
AAACCCTCGTGTGGTTAAATAACCAGTATCTTGAACAACGTTATCAGGAACTGAGTATGAAGAAGAATCTCACGAAAGAA
GAGCGGTTACAGCTTAGTAAGCTAATTTCAGTGATTAAAAACAAGGCTTAA

Upstream 100 bases:

>100_bases
GCGGCTTGTTTGTTTTCGAAATTCCCAAATTTATTTCACGTGATTTTTGATTTCACGTAACTTTTGATTTCACGTAAAAA
GGCGGCATTTAACAACACTA

Downstream 100 bases:

>100_bases
TTGAAAATACAGCCTCAAAAGTAAACACTTGGACTAGCACAGAGAAGCACCGAAGGCTATAATTGACGACTTGCGCGGCT
GTTTTTTGCCCGTCGTTTTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MAIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDF
VDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEASLFFQSQLRQHTDKQKVLDYLKHRGLSKEVVEHFNIGFAP
DGWDGLLSRYRQNQDSQDKLLTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRHREPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDKAGKEAAWRAL
ETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKQEFEAQIDQAMALPEFLFENLIKTYGNEDKSVLAKNAIKLIEKVQDT
VLQSLLLEDLAHKLRMNGADELKRKFNFSTKAAEKPNQHKALKGRGTPLRLAIALLVQHPHLGEKLPEQPALKHIQMAGI
DLLTLVLDLTRGQVITSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLNNQYLEQRYQELSMKKNLTKE
ERLQLSKLISVIKNKA

Sequences:

>Translated_576_residues
MAIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDF
VDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEASLFFQSQLRQHTDKQKVLDYLKHRGLSKEVVEHFNIGFAP
DGWDGLLSRYRQNQDSQDKLLTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRHREPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDKAGKEAAWRAL
ETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKQEFEAQIDQAMALPEFLFENLIKTYGNEDKSVLAKNAIKLIEKVQDT
VLQSLLLEDLAHKLRMNGADELKRKFNFSTKAAEKPNQHKALKGRGTPLRLAIALLVQHPHLGEKLPEQPALKHIQMAGI
DLLTLVLDLTRGQVITSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLNNQYLEQRYQELSMKKNLTKE
ERLQLSKLISVIKNKA
>Mature_575_residues
AIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDFV
DAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEASLFFQSQLRQHTDKQKVLDYLKHRGLSKEVVEHFNIGFAPD
GWDGLLSRYRQNQDSQDKLLTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKGN
ELYGLYELKQRHREPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDKAGKEAAWRALE
TALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKQEFEAQIDQAMALPEFLFENLIKTYGNEDKSVLAKNAIKLIEKVQDTV
LQSLLLEDLAHKLRMNGADELKRKFNFSTKAAEKPNQHKALKGRGTPLRLAIALLVQHPHLGEKLPEQPALKHIQMAGID
LLTLVLDLTRGQVITSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLNNQYLEQRYQELSMKKNLTKEE
RLQLSKLISVIKNKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=575, Percent_Identity=52.1739130434783, Blast_Score=580, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65730; Mature: 65599

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCF
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLDFVDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHH
SLFFQSQLRQHTDKQKVLDYLKHRGLSKEVVEHFNIGFAPDGWDGLLSRYRQNQDSQDKL
HHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHH
LTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHCEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
NELYGLYELKQRHREPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTAEQFQLLLRSAK
CCEEHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDKAGKEAAWRALETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKQEFEAQI
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DQAMALPEFLFENLIKTYGNEDKSVLAKNAIKLIEKVQDTVLQSLLLEDLAHKLRMNGAD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELKRKFNFSTKAAEKPNQHKALKGRGTPLRLAIALLVQHPHLGEKLPEQPALKHIQMAGI
HHHHHHCCCHHHCCCCCHHCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DLLTLVLDLTRGQVITSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQYLEQRYQELSMKKNLTKEERLQLSKLISVIKNKA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCF
CCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLDFVDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHH
SLFFQSQLRQHTDKQKVLDYLKHRGLSKEVVEHFNIGFAPDGWDGLLSRYRQNQDSQDKL
HHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHH
LTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHCEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
NELYGLYELKQRHREPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTAEQFQLLLRSAK
CCEEHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDKAGKEAAWRALETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKQEFEAQI
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DQAMALPEFLFENLIKTYGNEDKSVLAKNAIKLIEKVQDTVLQSLLLEDLAHKLRMNGAD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELKRKFNFSTKAAEKPNQHKALKGRGTPLRLAIALLVQHPHLGEKLPEQPALKHIQMAGI
HHHHHHCCCHHHCCCCCHHCHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DLLTLVLDLTRGQVITSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQYLEQRYQELSMKKNLTKEERLQLSKLISVIKNKA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]