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The map label for this gene is puuB [H]

Identifier: 167622939

GI number: 167622939

Start: 1228969

End: 1230291

Strand: Direct

Name: puuB [H]

Synonym: Shal_1000

Alternate gene names: 167622939

Gene position: 1228969-1230291 (Clockwise)

Preceding gene: 167622938

Following gene: 167622940

Centisome position: 23.51

GC content: 49.96

Gene sequence:

>1323_bases
ATGTCAATAATAACCGATGAAAGACAGGCGGAAACCATGATTACTACACCCCATGCAGATTCATACTATGCGGCGTCAGC
CAACGATAAGACCGAGCGTCCGATGCTACAAGATAATATCGAGACCGATATTTGCATTATTGGCGCCGGCTACACCGGAT
TATCGACCGCCTTGCACCTACTTGAGATGGGCTACAAGGTAACCGTACTCGAGGCGGCGCGTATTGGCTGGGGGGCGTCC
GGGCGTAATGGCGGCCAGATAGTTAACAGCTTTAGTCGTGATATCGACTCCATCGAAAAAACCGTAGGCACCGAGGCGGG
TAAGCTATTTGGTGAGATGGCCTTTGAAGGCGCTCGCATCATTAAAGAACGAATTAACAAGTACAATATTGATTGCGACC
TACAAGATGGTGGCGTGTTTGCCGCGCTAAACGATAAGCAGATGGGCCACCTGCAAGCGCAAAAAGCGCTGTGGGAAAAG
CATGGTCATATGAATCATCTCGAGCTGTTAGATAAAAATGACATTCGTAAGGTTGTCGACACAGAGTCTTATGTTGGCGG
TTTGCTAGATAAGAGTGGCGGCCATATTCACCCGCTCAACTTGGCGCTAGGTGAGGCGCAGGCTGTCGAGTCATTAGGTG
GGCAGATATTTGAAGGCTCGGCGGTGATTAAGGTCGAAGAGGGCGAAAATCCAGTGGTGCACACTAGCCATGGCAGCGTG
AAGTCTAAGTTTGTCGTGGTTGCGGGTAATGCCTACCTAGGCGGCTTGATGCCAAAGCTGCAAGCCAAGTCTATGCCTTG
TGGCACGCAGGTGATTACCACAGAGCCTTTAGATGAAGAGCTGGCTAAAAGCCTATTGCCACAGAACTACTGCGTCGAAG
ATTGTAACTACCTACTCGATTACTTTCGTCTATCGGGTGATAACCGTCTTATTTATGGCGGCGGCGTGGTGTATGGTGCA
AGAGATCCTGCGGATATTAAGTCGATCATCGGACCTAAGATGCTCCAGACTTTTCCTCAGCTTAAAGGTGTGAATATCGA
TTACACCTGGACCGGCAACTTCCTGCTGACCCTGTCTCGTTTGCCACAGGTGGGGCGCATTGGTAACAATATTTACTATT
CACAAGGTTGTAGTGGTCATGGTGTTACTTACACTCACTTGGCGGGCAAGATCTTAGCCGAGGCGATTAACGGTCAGGCG
AGCAGATTTGATGCCTTCGCAGGCTTGCCGCACTATCCGTTCCCTGGTGGGCATATGTTTAAGGTGCCCTTTAGCGCCAT
TGGTGCTTGGTACTACACCATGCGCGACAAGTTAGGTGTATAA

Upstream 100 bases:

>100_bases
TACCCCTTATTTGTTGAAAACTCATCTGTTAAAGATAGGCGCTCAGCTATCGTGATAAAGAGATCCGCGGCTAAGGGTAA
AGAACAAAAGGACAGAAGTT

Downstream 100 bases:

>100_bases
CTATTAGGGGGTATAAATTTATACCTTTATGATGAACTCAACTCGGCTTTAGGGGGTCGAAGACTATACCAATCAGATAT
TTAATATGATTGATATTAGT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSIITDERQAETMITTPHADSYYAASANDKTERPMLQDNIETDICIIGAGYTGLSTALHLLEMGYKVTVLEAARIGWGAS
GRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARIIKERINKYNIDCDLQDGGVFAALNDKQMGHLQAQKALWEK
HGHMNHLELLDKNDIRKVVDTESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGENPVVHTSHGSV
KSKFVVVAGNAYLGGLMPKLQAKSMPCGTQVITTEPLDEELAKSLLPQNYCVEDCNYLLDYFRLSGDNRLIYGGGVVYGA
RDPADIKSIIGPKMLQTFPQLKGVNIDYTWTGNFLLTLSRLPQVGRIGNNIYYSQGCSGHGVTYTHLAGKILAEAINGQA
SRFDAFAGLPHYPFPGGHMFKVPFSAIGAWYYTMRDKLGV

Sequences:

>Translated_440_residues
MSIITDERQAETMITTPHADSYYAASANDKTERPMLQDNIETDICIIGAGYTGLSTALHLLEMGYKVTVLEAARIGWGAS
GRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARIIKERINKYNIDCDLQDGGVFAALNDKQMGHLQAQKALWEK
HGHMNHLELLDKNDIRKVVDTESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGENPVVHTSHGSV
KSKFVVVAGNAYLGGLMPKLQAKSMPCGTQVITTEPLDEELAKSLLPQNYCVEDCNYLLDYFRLSGDNRLIYGGGVVYGA
RDPADIKSIIGPKMLQTFPQLKGVNIDYTWTGNFLLTLSRLPQVGRIGNNIYYSQGCSGHGVTYTHLAGKILAEAINGQA
SRFDAFAGLPHYPFPGGHMFKVPFSAIGAWYYTMRDKLGV
>Mature_439_residues
SIITDERQAETMITTPHADSYYAASANDKTERPMLQDNIETDICIIGAGYTGLSTALHLLEMGYKVTVLEAARIGWGASG
RNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARIIKERINKYNIDCDLQDGGVFAALNDKQMGHLQAQKALWEKH
GHMNHLELLDKNDIRKVVDTESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGENPVVHTSHGSVK
SKFVVVAGNAYLGGLMPKLQAKSMPCGTQVITTEPLDEELAKSLLPQNYCVEDCNYLLDYFRLSGDNRLIYGGGVVYGAR
DPADIKSIIGPKMLQTFPQLKGVNIDYTWTGNFLLTLSRLPQVGRIGNNIYYSQGCSGHGVTYTHLAGKILAEAINGQAS
RFDAFAGLPHYPFPGGHMFKVPFSAIGAWYYTMRDKLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=62.4413145539906, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47885; Mature: 47754

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIITDERQAETMITTPHADSYYAASANDKTERPMLQDNIETDICIIGAGYTGLSTALHL
CCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHH
LEMGYKVTVLEAARIGWGASGRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARI
HHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IKERINKYNIDCDLQDGGVFAALNDKQMGHLQAQKALWEKHGHMNHLELLDKNDIRKVVD
HHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHC
TESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGENPVVHTSHGSV
CHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHCCEEECCCEEEEEECCCCCEEECCCCCC
KSKFVVVAGNAYLGGLMPKLQAKSMPCGTQVITTEPLDEELAKSLLPQNYCVEDCNYLLD
CEEEEEEECCHHHHCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHH
YFRLSGDNRLIYGGGVVYGARDPADIKSIIGPKMLQTFPQLKGVNIDYTWTGNFLLTLSR
HHHCCCCCEEEECCEEEECCCCHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCEEEEEHH
LPQVGRIGNNIYYSQGCSGHGVTYTHLAGKILAEAINGQASRFDAFAGLPHYPFPGGHMF
CCCHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEE
KVPFSAIGAWYYTMRDKLGV
ECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIITDERQAETMITTPHADSYYAASANDKTERPMLQDNIETDICIIGAGYTGLSTALHL
CCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHH
LEMGYKVTVLEAARIGWGASGRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARI
HHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IKERINKYNIDCDLQDGGVFAALNDKQMGHLQAQKALWEKHGHMNHLELLDKNDIRKVVD
HHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHC
TESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGENPVVHTSHGSV
CHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHCCEEECCCEEEEEECCCCCEEECCCCCC
KSKFVVVAGNAYLGGLMPKLQAKSMPCGTQVITTEPLDEELAKSLLPQNYCVEDCNYLLD
CEEEEEEECCHHHHCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHCCCCCCHHHHHHHHH
YFRLSGDNRLIYGGGVVYGARDPADIKSIIGPKMLQTFPQLKGVNIDYTWTGNFLLTLSR
HHHCCCCCEEEECCEEEECCCCHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCEEEEEHH
LPQVGRIGNNIYYSQGCSGHGVTYTHLAGKILAEAINGQASRFDAFAGLPHYPFPGGHMF
CCCHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEE
KVPFSAIGAWYYTMRDKLGV
ECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]