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The map label for this gene is nemA [H]

Identifier: 167622896

GI number: 167622896

Start: 1177996

End: 1179033

Strand: Reverse

Name: nemA [H]

Synonym: Shal_0956

Alternate gene names: 167622896

Gene position: 1179033-1177996 (Counterclockwise)

Preceding gene: 167622898

Following gene: 167622894

Centisome position: 22.56

GC content: 44.51

Gene sequence:

>1038_bases
ATGAAATACGCCCCCTTGTTTGAACCATATAAGCTAAGCGAACAACTGACACTAAAGAATCGTATAGTGATGGCGCCGAT
GAATCGTAGTATGGCTGATGAGGAATTTCTCCCTACAGCAGCGATGATGAACTATTACGCTGGTCGGGCCGATACCGGTT
TAATTATTTCCGAGTCGCTTATGATCTCCCAAAATGCCCAAGGCTATCCCAATACACCCGCAATCTATTTAGCTGAACAT
GTCGCTGGCTGGAGAAAAATCACCGACAAGGTACATGAAAACGACGGTAAAATTTTTGCTCAGTTATGGCATACGGGCAG
AGTTTCGCACCCTATTTATTTAGCAGGCCAGCAACCATGGGCTCCCTCAGCAGTGCCACTTGAGGGGCGTGTGCCTCGCA
CCGATAATTTGCAATATGGCATCCCCAAAGAGATGACGGAGCAGGAGATAAAGCTAATCATTACTGAGTTTGCTAATGCC
GCAGCCAATGCAAAAGAGGCTGGTTTTGACGGGGTCGAGATCCATGCTGCAAACGGTTATCTACTCGATCAGTTCTTACA
TTGGGATACCAACCGACGCACTGATGCTTGGGGGGGAAGCGAAGAGAATATGAGCCGAATATTGTTTGAGGTTATCGATG
CGGTAAAACAAAACATTACTCATGTTGGCGTCCGCTTGTCTCCCGTTGCTTATCTGAATCTTAAACATGATGAACGGGAT
ATTCTTGTTTCTAACTACCTACTTCAACGTCTAAATGATTGTAATTTAAGCTACGTTCATACAGGTATGTTCGAAGACAC
TTATCAAGCGCACTTACAAGGTAGCGTAACCCAGTACATTAAGAGTCTATATCACGGAACCATTATCGCTTGTGGTGGCT
ATAACGCAGAAAGTGGCAGCATAGCCATAAATCAAGGTGATGCTAACTTAGTCGCAATTGGAAGACCACTGATCGCTAAC
CCTGATTATGTTGAGAAAATACGCACTAAAAAGCCACTAACCGTCTACGATGCAGAGATGTTAAATCAACTCATTTAG

Upstream 100 bases:

>100_bases
ATTAACACTATTCATTTCAGTCTAGTTCTATTCGCATTTACTGTCACCTATAGTTATCTAACACAATCTAATTTCTTTTC
ATCTTCTCAGAGATCCTCTA

Downstream 100 bases:

>100_bases
GCCTTGCCGATGATTAAAAACAACCGCGACAAGGGAGTCCTATTTTCCAATCTAGACCGCTATTTTAAACTGATTATTTA
GCGGGAAATTTTGTCCGTTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKYAPLFEPYKLSEQLTLKNRIVMAPMNRSMADEEFLPTAAMMNYYAGRADTGLIISESLMISQNAQGYPNTPAIYLAEH
VAGWRKITDKVHENDGKIFAQLWHTGRVSHPIYLAGQQPWAPSAVPLEGRVPRTDNLQYGIPKEMTEQEIKLIITEFANA
AANAKEAGFDGVEIHAANGYLLDQFLHWDTNRRTDAWGGSEENMSRILFEVIDAVKQNITHVGVRLSPVAYLNLKHDERD
ILVSNYLLQRLNDCNLSYVHTGMFEDTYQAHLQGSVTQYIKSLYHGTIIACGGYNAESGSIAINQGDANLVAIGRPLIAN
PDYVEKIRTKKPLTVYDAEMLNQLI

Sequences:

>Translated_345_residues
MKYAPLFEPYKLSEQLTLKNRIVMAPMNRSMADEEFLPTAAMMNYYAGRADTGLIISESLMISQNAQGYPNTPAIYLAEH
VAGWRKITDKVHENDGKIFAQLWHTGRVSHPIYLAGQQPWAPSAVPLEGRVPRTDNLQYGIPKEMTEQEIKLIITEFANA
AANAKEAGFDGVEIHAANGYLLDQFLHWDTNRRTDAWGGSEENMSRILFEVIDAVKQNITHVGVRLSPVAYLNLKHDERD
ILVSNYLLQRLNDCNLSYVHTGMFEDTYQAHLQGSVTQYIKSLYHGTIIACGGYNAESGSIAINQGDANLVAIGRPLIAN
PDYVEKIRTKKPLTVYDAEMLNQLI
>Mature_345_residues
MKYAPLFEPYKLSEQLTLKNRIVMAPMNRSMADEEFLPTAAMMNYYAGRADTGLIISESLMISQNAQGYPNTPAIYLAEH
VAGWRKITDKVHENDGKIFAQLWHTGRVSHPIYLAGQQPWAPSAVPLEGRVPRTDNLQYGIPKEMTEQEIKLIITEFANA
AANAKEAGFDGVEIHAANGYLLDQFLHWDTNRRTDAWGGSEENMSRILFEVIDAVKQNITHVGVRLSPVAYLNLKHDERD
ILVSNYLLQRLNDCNLSYVHTGMFEDTYQAHLQGSVTQYIKSLYHGTIIACGGYNAESGSIAINQGDANLVAIGRPLIAN
PDYVEKIRTKKPLTVYDAEMLNQLI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=353, Percent_Identity=38.2436260623229, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=29.6829971181556, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17559804, Length=326, Percent_Identity=30.6748466257669, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17565138, Length=329, Percent_Identity=28.8753799392097, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=357, Percent_Identity=29.9719887955182, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=319, Percent_Identity=28.8401253918495, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17564188, Length=319, Percent_Identity=28.2131661442006, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17540738, Length=349, Percent_Identity=25.7879656160458, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=358, Percent_Identity=25.6983240223464, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=367, Percent_Identity=33.2425068119891, Blast_Score=155, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6325086, Length=360, Percent_Identity=33.0555555555556, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38688; Mature: 38688

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYAPLFEPYKLSEQLTLKNRIVMAPMNRSMADEEFLPTAAMMNYYAGRADTGLIISESL
CCCCCCCCCHHCCCCEEECCEEEECCCCCCCCCHHHCCHHHHHHHHCCCCCCCEEEECEE
MISQNAQGYPNTPAIYLAEHVAGWRKITDKVHENDGKIFAQLWHTGRVSHPIYLAGQQPW
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCEEEECCCCC
APSAVPLEGRVPRTDNLQYGIPKEMTEQEIKLIITEFANAAANAKEAGFDGVEIHAANGY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCH
LLDQFLHWDTNRRTDAWGGSEENMSRILFEVIDAVKQNITHVGVRLSPVAYLNLKHDERD
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEEEECCCCHH
ILVSNYLLQRLNDCNLSYVHTGMFEDTYQAHLQGSVTQYIKSLYHGTIIACGGYNAESGS
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCC
IAINQGDANLVAIGRPLIANPDYVEKIRTKKPLTVYDAEMLNQLI
EEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCEEECHHHHHHCC
>Mature Secondary Structure
MKYAPLFEPYKLSEQLTLKNRIVMAPMNRSMADEEFLPTAAMMNYYAGRADTGLIISESL
CCCCCCCCCHHCCCCEEECCEEEECCCCCCCCCHHHCCHHHHHHHHCCCCCCCEEEECEE
MISQNAQGYPNTPAIYLAEHVAGWRKITDKVHENDGKIFAQLWHTGRVSHPIYLAGQQPW
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCEEEECCCCC
APSAVPLEGRVPRTDNLQYGIPKEMTEQEIKLIITEFANAAANAKEAGFDGVEIHAANGY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCH
LLDQFLHWDTNRRTDAWGGSEENMSRILFEVIDAVKQNITHVGVRLSPVAYLNLKHDERD
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEEEECCCCHH
ILVSNYLLQRLNDCNLSYVHTGMFEDTYQAHLQGSVTQYIKSLYHGTIIACGGYNAESGS
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCC
IAINQGDANLVAIGRPLIANPDYVEKIRTKKPLTVYDAEMLNQLI
EEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]