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The map label for this gene is lptD [H]

Identifier: 167622858

GI number: 167622858

Start: 1137178

End: 1139502

Strand: Reverse

Name: lptD [H]

Synonym: Shal_0918

Alternate gene names: 167622858

Gene position: 1139502-1137178 (Counterclockwise)

Preceding gene: 167622864

Following gene: 167622857

Centisome position: 21.8

GC content: 44.99

Gene sequence:

>2325_bases
ATGCAGATCCGTTATTTTCTGGCCTTGAGCCTGCTTCCCCAATTAGTCTTAGCAGACGAACCCGAAACTGTGACGCCACT
TGCGTCGCAGTGCGTAGTTGAACCTCCGGTAAAGCCGTATGTACCGCTTTACGATATCAATAATCCAGCCGACTTAGCGC
GGATCTATATTAGCTCTGATAGTTCTTCAGCCCAGATGGGTAAAGAAGCAACTTTCAATGGTGATGTAAACTTCAGTCAG
GGCGGCCGTAATATCGCTGCCGATGAAGCGATTTTGAATCAGCAAACTGAAGAACTTCATGCCAATGGTAATTTAGTTTT
TCAAGACTCTATGTTTACTGTCACCGCCGACTCGTTAGTCGCGAAGATGCGCAGTAACAGTGCCGTATTGAGTGGCGCGC
AATATTGGCTTACCGGGCAACAAATTCACGGCGATGCCGAAAAGCTTGAGATCACTACAGATAACAACCTATTGCTGTCT
GGTACTAACTTCACGACTTGCCCAGGTAAAGATCCAGCCTGGCTGCTAGAAGCCGATCGCATCAAGATTGATAGCTCTGA
AGAGTGGGGCGAGATCTGGGACGCTAAGATTAAGATTGCAGGTATCCCTGTGATGTATGTCCCCTACATGACCATCCCGG
TATCTGATAAGCGTAAATCAGGCTTTTTATTCCCAACATTTAGCACCAGTACTACCAACGGTGTTGAAGTGGCGACGCCT
TATTACTGGAATATCGCACCAGAATACGACTTAACTTTTACGCCAAATTACATGTCTGCTCGTGGACTATTTACCAAGAC
AGAGTTTAGGTATTTAGCTGGCGAGAAACAGCATGGTCAGTTAAATTTCGAATATCTAGCCAACGATAATAAGCTAGATA
ATAGCCCTGATAGATACCTGTATCACTGGGATCATAGCGGCGCGATTAATGAAAACTGGCGCGTACTGGCTAATTATACC
GAAGTATCGGATAACAACTATTTTAACGACTTAAACTCTGACGTTCAGCGCGCCACCGATAACCAATTAAACCGCATTGG
TGAGATTGCTTATTTTGAACAAAACTGGGATTTCAGTGCGCGAGTTCAAGATATTAAAGTGCTGGGTGAAGAGGAAAAAC
CTTATCAGGTGATGCCGCAAGTTACCTTTAACTACCGTGCACCAAGCTATTGGCATGGCTTAGACTTTAAGCTTTATAGT
GAAGCGACTAATTTCGAGCATCAAGATAGAGAATATTCAGCTGCGACTCGTGTGCACCTTGAGCCAAGCATCTCTTACCC
AATTCATGGTCCAGCGGGCTCACTAACCAGTGAAGTCAAGCTACTGCAAACTAACTATTGGCAGGATGACAGGTCAGATC
TCTCTGATTCAGAACTTCGCGACCGAGTTAACCGAACACTGCCAGAAGTTCGTATTCATGGTCAGGTGAACTTTGAGCGC
TTTACGGATTACTTCGATACTAACTATCGCCAAACATTAGAGCCGCAGTTCCAATACCTTTATGTTGGCTACCAAGATCA
ATCTGATATCGGGATTTACGATACCGCACCGTTGCAAGAGGATTACAACGGTCTATTTAGAGATCGTCGCTTTTCAGGTT
TAGATCGCATCGCCGATGCCAACCAGTTTACGCTCGGCTTAACCACTAAGCTGTTTAACGAACAAAACCGCGAAGTCTTT
AAATTCAGTCTCGGACAAATTATTTACCTAGAAGACAGCCGTGTAGGTATTAACGACATCCCGGGTAGTAACGATACCTT
CACTCAAGAGGGAACGTCCAACTCAGCCATTGCAGCCGAGCTGAGCACCCAACTCTACGCCGACTGGTTTTTAAGTGGCT
CTATTCAGTACGACGCTAAAGAGAGTGAAAATAAGAAGAGCGAAGTCACCGTTGACTACCGCCCTGGTACCAACAAGCTG
TTGCAAGCAAGCTACCGCTACGTACCCGATCTACTGAACACTAACACCAATGAGTCTGTCGATATTAAGCAGCTGGGTTT
CCGTGGAGCCTGGCCGATTAACGACAGTCTCTATTTAGTGGGTAACTGGTATTACGATCTCAATGAAAGCAGAGCTGTAG
AAACCTATACCGGTTTTCAATATGAATCATGTTGCTGGGCAGTGCGTTTGAGTTATCATTACCGCATCAAGACCAATTAT
ATTGACGAAGATAATCCGGTACAGGATACTCGAGAGCTATTTGAAAGTGGGATCTACCTGAACTTTATCATCAAAGGACT
CGGTGGTTCTGGCCCATTAGGGGTATCAGATATGTTAGATGACGGCCTATTTAACTATCGAAAACCGCTTTATTTGAAGA
ATTAG

Upstream 100 bases:

>100_bases
AGCAATTGAAAAAAATTATTTGGCTGAGTTGCCCAGAAAAGGGTCAACTTTTCTCCTCTTTCACCGTCCAGACTAAGAAA
TCATAATTAATTGACCTAAG

Downstream 100 bases:

>100_bases
CAAATAAATAACGTAAATAAATGCTGAACCTAGCTGACGACATGCAGTCAAAGCAGAACTAAAGAATCATTAACCCGGTG
AGTATTTGCCGTAAATGACA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MQIRYFLALSLLPQLVLADEPETVTPLASQCVVEPPVKPYVPLYDINNPADLARIYISSDSSSAQMGKEATFNGDVNFSQ
GGRNIAADEAILNQQTEELHANGNLVFQDSMFTVTADSLVAKMRSNSAVLSGAQYWLTGQQIHGDAEKLEITTDNNLLLS
GTNFTTCPGKDPAWLLEADRIKIDSSEEWGEIWDAKIKIAGIPVMYVPYMTIPVSDKRKSGFLFPTFSTSTTNGVEVATP
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQHGQLNFEYLANDNKLDNSPDRYLYHWDHSGAINENWRVLANYT
EVSDNNYFNDLNSDVQRATDNQLNRIGEIAYFEQNWDFSARVQDIKVLGEEEKPYQVMPQVTFNYRAPSYWHGLDFKLYS
EATNFEHQDREYSAATRVHLEPSISYPIHGPAGSLTSEVKLLQTNYWQDDRSDLSDSELRDRVNRTLPEVRIHGQVNFER
FTDYFDTNYRQTLEPQFQYLYVGYQDQSDIGIYDTAPLQEDYNGLFRDRRFSGLDRIADANQFTLGLTTKLFNEQNREVF
KFSLGQIIYLEDSRVGINDIPGSNDTFTQEGTSNSAIAAELSTQLYADWFLSGSIQYDAKESENKKSEVTVDYRPGTNKL
LQASYRYVPDLLNTNTNESVDIKQLGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
IDEDNPVQDTRELFESGIYLNFIIKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN

Sequences:

>Translated_774_residues
MQIRYFLALSLLPQLVLADEPETVTPLASQCVVEPPVKPYVPLYDINNPADLARIYISSDSSSAQMGKEATFNGDVNFSQ
GGRNIAADEAILNQQTEELHANGNLVFQDSMFTVTADSLVAKMRSNSAVLSGAQYWLTGQQIHGDAEKLEITTDNNLLLS
GTNFTTCPGKDPAWLLEADRIKIDSSEEWGEIWDAKIKIAGIPVMYVPYMTIPVSDKRKSGFLFPTFSTSTTNGVEVATP
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQHGQLNFEYLANDNKLDNSPDRYLYHWDHSGAINENWRVLANYT
EVSDNNYFNDLNSDVQRATDNQLNRIGEIAYFEQNWDFSARVQDIKVLGEEEKPYQVMPQVTFNYRAPSYWHGLDFKLYS
EATNFEHQDREYSAATRVHLEPSISYPIHGPAGSLTSEVKLLQTNYWQDDRSDLSDSELRDRVNRTLPEVRIHGQVNFER
FTDYFDTNYRQTLEPQFQYLYVGYQDQSDIGIYDTAPLQEDYNGLFRDRRFSGLDRIADANQFTLGLTTKLFNEQNREVF
KFSLGQIIYLEDSRVGINDIPGSNDTFTQEGTSNSAIAAELSTQLYADWFLSGSIQYDAKESENKKSEVTVDYRPGTNKL
LQASYRYVPDLLNTNTNESVDIKQLGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
IDEDNPVQDTRELFESGIYLNFIIKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN
>Mature_774_residues
MQIRYFLALSLLPQLVLADEPETVTPLASQCVVEPPVKPYVPLYDINNPADLARIYISSDSSSAQMGKEATFNGDVNFSQ
GGRNIAADEAILNQQTEELHANGNLVFQDSMFTVTADSLVAKMRSNSAVLSGAQYWLTGQQIHGDAEKLEITTDNNLLLS
GTNFTTCPGKDPAWLLEADRIKIDSSEEWGEIWDAKIKIAGIPVMYVPYMTIPVSDKRKSGFLFPTFSTSTTNGVEVATP
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQHGQLNFEYLANDNKLDNSPDRYLYHWDHSGAINENWRVLANYT
EVSDNNYFNDLNSDVQRATDNQLNRIGEIAYFEQNWDFSARVQDIKVLGEEEKPYQVMPQVTFNYRAPSYWHGLDFKLYS
EATNFEHQDREYSAATRVHLEPSISYPIHGPAGSLTSEVKLLQTNYWQDDRSDLSDSELRDRVNRTLPEVRIHGQVNFER
FTDYFDTNYRQTLEPQFQYLYVGYQDQSDIGIYDTAPLQEDYNGLFRDRRFSGLDRIADANQFTLGLTTKLFNEQNREVF
KFSLGQIIYLEDSRVGINDIPGSNDTFTQEGTSNSAIAAELSTQLYADWFLSGSIQYDAKESENKKSEVTVDYRPGTNKL
LQASYRYVPDLLNTNTNESVDIKQLGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
IDEDNPVQDTRELFESGIYLNFIIKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=803, Percent_Identity=33.3748443337484, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88504; Mature: 88504

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRYFLALSLLPQLVLADEPETVTPLASQCVVEPPVKPYVPLYDINNPADLARIYISSD
CEEEEHHHHHHHHHHHCCCCCCCCCCHHHHCEECCCCCCCEEEEECCCCCCEEEEEEECC
SSSAQMGKEATFNGDVNFSQGGRNIAADEAILNQQTEELHANGNLVFQDSMFTVTADSLV
CCHHHCCCCCCCCCCCEECCCCCCCCHHHHHHCCCHHHHCCCCCEEEECCEEEEEHHHHH
AKMRSNSAVLSGAQYWLTGQQIHGDAEKLEITTDNNLLLSGTNFTTCPGKDPAWLLEADR
HHHCCCCEEECCCEEEEECCEECCCCCEEEEEECCCEEEECCCEEECCCCCCCEEEEECE
IKIDSSEEWGEIWDAKIKIAGIPVMYVPYMTIPVSDKRKSGFLFPTFSTSTTNGVEVATP
EEECCCCCCHHHCCCEEEEECCCEEEECEEEEECCCCCCCCEEEEECCCCCCCCEEEECC
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQHGQLNFEYLANDNKLDNSPDRYL
EEEECCCCCCEEECCCCCCCCCCEEECHHHEECCCCCCCCEEEEEEECCCCCCCCCCCEE
YHWDHSGAINENWRVLANYTEVSDNNYFNDLNSDVQRATDNQLNRIGEIAYFEQNWDFSA
EEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
RVQDIKVLGEEEKPYQVMPQVTFNYRAPSYWHGLDFKLYSEATNFEHQDREYSAATRVHL
EEEEEEEECCCCCCEEECCEEEEECCCCCEECCCCEEEECCCCCCCCCCCCCCCEEEEEE
EPSISYPIHGPAGSLTSEVKLLQTNYWQDDRSDLSDSELRDRVNRTLPEVRIHGQVNFER
CCCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECHHH
FTDYFDTNYRQTLEPQFQYLYVGYQDQSDIGIYDTAPLQEDYNGLFRDRRFSGLDRIADA
HHHHHCCCCHHCCCCCEEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCHHHHCCC
NQFTLGLTTKLFNEQNREVFKFSLGQIIYLEDSRVGINDIPGSNDTFTQEGTSNSAIAAE
CEEEEEEEHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEH
LSTQLYADWFLSGSIQYDAKESENKKSEVTVDYRPGTNKLLQASYRYVPDLLNTNTNESV
HHHHHEEEEEECCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
DIKQLGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
EHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEEEEEEEECC
IDEDNPVQDTRELFESGIYLNFIIKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCHHHCCCCEEECC
>Mature Secondary Structure
MQIRYFLALSLLPQLVLADEPETVTPLASQCVVEPPVKPYVPLYDINNPADLARIYISSD
CEEEEHHHHHHHHHHHCCCCCCCCCCHHHHCEECCCCCCCEEEEECCCCCCEEEEEEECC
SSSAQMGKEATFNGDVNFSQGGRNIAADEAILNQQTEELHANGNLVFQDSMFTVTADSLV
CCHHHCCCCCCCCCCCEECCCCCCCCHHHHHHCCCHHHHCCCCCEEEECCEEEEEHHHHH
AKMRSNSAVLSGAQYWLTGQQIHGDAEKLEITTDNNLLLSGTNFTTCPGKDPAWLLEADR
HHHCCCCEEECCCEEEEECCEECCCCCEEEEEECCCEEEECCCEEECCCCCCCEEEEECE
IKIDSSEEWGEIWDAKIKIAGIPVMYVPYMTIPVSDKRKSGFLFPTFSTSTTNGVEVATP
EEECCCCCCHHHCCCEEEEECCCEEEECEEEEECCCCCCCCEEEEECCCCCCCCEEEECC
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQHGQLNFEYLANDNKLDNSPDRYL
EEEECCCCCCEEECCCCCCCCCCEEECHHHEECCCCCCCCEEEEEEECCCCCCCCCCCEE
YHWDHSGAINENWRVLANYTEVSDNNYFNDLNSDVQRATDNQLNRIGEIAYFEQNWDFSA
EEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
RVQDIKVLGEEEKPYQVMPQVTFNYRAPSYWHGLDFKLYSEATNFEHQDREYSAATRVHL
EEEEEEEECCCCCCEEECCEEEEECCCCCEECCCCEEEECCCCCCCCCCCCCCCEEEEEE
EPSISYPIHGPAGSLTSEVKLLQTNYWQDDRSDLSDSELRDRVNRTLPEVRIHGQVNFER
CCCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECHHH
FTDYFDTNYRQTLEPQFQYLYVGYQDQSDIGIYDTAPLQEDYNGLFRDRRFSGLDRIADA
HHHHHCCCCHHCCCCCEEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCHHHHCCC
NQFTLGLTTKLFNEQNREVFKFSLGQIIYLEDSRVGINDIPGSNDTFTQEGTSNSAIAAE
CEEEEEEEHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEH
LSTQLYADWFLSGSIQYDAKESENKKSEVTVDYRPGTNKLLQASYRYVPDLLNTNTNESV
HHHHHEEEEEECCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
DIKQLGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
EHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEEEEEEEECC
IDEDNPVQDTRELFESGIYLNFIIKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA