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The map label for this gene is mdh [H]

Identifier: 167622843

GI number: 167622843

Start: 1123713

End: 1124648

Strand: Direct

Name: mdh [H]

Synonym: Shal_0903

Alternate gene names: 167622843

Gene position: 1123713-1124648 (Clockwise)

Preceding gene: 167622840

Following gene: 167622845

Centisome position: 21.5

GC content: 47.12

Gene sequence:

>936_bases
ATGAAAGTTGCTGTACTTGGTGCTGCTGGTGGTATTGGCCAGGCGCTCGCCCTACTGTTAAAAACTCAATTACCTGCGGG
TTCAAAATTGTCGCTTTATGATATCGCTCCAGTTATACCTGGTGTTGCGGTTGACTTAAGCCACATCCCAACGGCGGTTG
AAGTTAAAGGTTTCGCTGGTGAAGACCCTACGGCAGCACTAGAAGGTGCTGATGTTGTGCTTATCTCTGCCGGTGTTGCA
CGTAAGCCTGGGATGGATCGTTCCGATCTGTTTAACATCAACGCAGGTATCGTGCGTAACTTGGTTGAAAAGTGCGCTGC
GACTTGCCCTAAGGCATTAATAGGTATTATTACCAACCCGGTAAACACAACGGTTGCGATTGCAGCTGAAGTGCTTAAAA
AGGCGGGTGTTTATGATAAAAATCGCCTGTTTGGTGTGACGACACTCGATGTTATCCGTTCAGAAACCTTCGTTGCTGAA
GCAAAAGACCTAAATGTTGCTGACGTTAAAGTTCCTGTCATCGGCGGTCATAGTGGTGTGACGATTCTACCTCTACTTTC
ACAGGTTGAAGGCGTAAGCTTTACTGATGCAGAAATTGCAGCACTGACGACTCGTATCCAGAATGCGGGGACTGAGGTGG
TTGAGGCTAAAGCCGGTGGTGGTAGTGCAACATTATCTATGGGTCAAGCTGCATGTCGCTTTGGTCTATCACTTGTTCGT
GGTCTTCAGGGTGAAGCAAATGTTGTTGAGTGTGCCTATGTTGATGGTGGCAGTGAGCATACTGAGTTCTTCGCACAACC
TGTTGTACTCGGCAAGAACGGCATTGAACAGGTACTTGCTTATGGCGAGGTAAGTGCATTTGAAGCTAATGCTCGTGACG
CTATGCTAGACACTCTAAAGGCTGATATTCAGCTAGGTATCGAATTCGTTAAGTAA

Upstream 100 bases:

>100_bases
GACTAAGGGATAATTGAATTTTTTTTGTGAATCCAGTACTGTTGCCACATTGGAAAGATGGATCTGAATCACAAAAATCC
AAGAATTGATGGAGATGATT

Downstream 100 bases:

>100_bases
TCGATGCAAGGGCCTTTGTGGGTCAAAATAAAAAAGGAGCCTTAGGCTCCTTTTTTATTTTCAACATTTATCTCGTCACT
GCTCTTAGCTTACAGGTGGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVIPGVAVDLSHIPTAVEVKGFAGEDPTAALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAE
AKDLNVADVKVPVIGGHSGVTILPLLSQVEGVSFTDAEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANVVECAYVDGGSEHTEFFAQPVVLGKNGIEQVLAYGEVSAFEANARDAMLDTLKADIQLGIEFVK

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVIPGVAVDLSHIPTAVEVKGFAGEDPTAALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAE
AKDLNVADVKVPVIGGHSGVTILPLLSQVEGVSFTDAEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANVVECAYVDGGSEHTEFFAQPVVLGKNGIEQVLAYGEVSAFEANARDAMLDTLKADIQLGIEFVK
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVIPGVAVDLSHIPTAVEVKGFAGEDPTAALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAE
AKDLNVADVKVPVIGGHSGVTILPLLSQVEGVSFTDAEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANVVECAYVDGGSEHTEFFAQPVVLGKNGIEQVLAYGEVSAFEANARDAMLDTLKADIQLGIEFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=56.4102564102564, Blast_Score=324, Evalue=8e-89,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=80.3225806451613, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=57.8274760383387, Blast_Score=328, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=50.1587301587302, Blast_Score=275, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=37.5739644970414, Blast_Score=187, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324446, Length=368, Percent_Identity=36.9565217391304, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=55.8064516129032, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=48.8817891373802, Blast_Score=270, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=44.3333333333333, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI17136226, Length=326, Percent_Identity=23.9263803680982, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 31979; Mature: 31979

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVIPGVAVDLSHIPTAVEVKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEHEEHHHHCCCCEEEHHHCCCEEEEECCCC
EDPTAALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAEAKDLNVADVKVPVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHEECCCCCCEEEEEEEEEECCCCC
TILPLLSQVEGVSFTDAEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHTEFFAQPVVLGKNGIEQVLAYGEVSAFEANARDAMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCEEECCCCHHHHHHHCCHHHCCCCCHHHHHHHHH
ADIQLGIEFVK
HHHHHCEEECC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVIPGVAVDLSHIPTAVEVKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEHEEHHHHCCCCEEEHHHCCCEEEEECCCC
EDPTAALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLVEKCAATCPKALIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRSETFVAEAKDLNVADVKVPVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHEECCCCCCEEEEEEEEEECCCCC
TILPLLSQVEGVSFTDAEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHTEFFAQPVVLGKNGIEQVLAYGEVSAFEANARDAMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCEEECCCCHHHHHHHCCHHHCCCCCHHHHHHHHH
ADIQLGIEFVK
HHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA