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The map label for this gene is braB [H]

Identifier: 167622799

GI number: 167622799

Start: 1070680

End: 1072023

Strand: Direct

Name: braB [H]

Synonym: Shal_0859

Alternate gene names: 167622799

Gene position: 1070680-1072023 (Clockwise)

Preceding gene: 167622796

Following gene: 167622800

Centisome position: 20.48

GC content: 45.61

Gene sequence:

>1344_bases
ATGGGGCATAAAGTGCAAAACAATCAAATGAGTATCGCAGATACGTTAGGGTTAGGTTTCATGACCTTCGCGTTCTTCTT
AGGCGCGGGTAACTTAATTTTCCCACCGTTAGCTGGCTTTTTGGCGGGCGAGAATATCACGCTAGCCATGTTTGGCTTCC
TTATTACTGCGGTTGGTCTTCCTCTCCTAACTCTTATCGCTGTCGCTAAAGCGAACGGTAAAATAATGCAATTGCTGCCT
CCCATCGCTGCAACTGCATTAGCCGTCTCAATTTTCATTATTATAGGCCCTGCGTTCGCCGCACCAAGAACTGGACTCGT
TGCCTACGAAGTGGGCTTTAAGCCGTTTCTAACAGACACTAGTGCAACCTTTATGATTGCGGGTTTAGCTTTTAACCTCA
CTCAGCTTATTTATACCTTAGTTTTCTTCGGTCTCTCTATGGTGCTTGCTCTGTTCCCTGGCAAGTTACTTGATAGCGTA
GGTAAAGTATTAACCCCAATCCTTATTTTACTTTTAGTGGGTCTAGCTATTTCAGTTATTGTTATTCCTGGTAGCGACAT
TGGCGCAGCGGTTGGTGATTACCAAGCAAACCCGCTAACTAAGGGGATCTTGGAAGGCTATAACACTATGGATACCTTGG
CCTCTTTGATCTTCGGTATGTTGATCATCGATATCTTACGTAAGAAAGGCATCACAGAGCCAAAAGCACAGACTAACTAT
TTAATTAAAGCGGCTTTGATTGCGGCTGCAGGCTTGGCATTTGTTTATATATCACTATTCTATTTAGGTGCAACGGCGGG
CGACCTCGCTACTGGGGCTGAGAATGGCGGCGTGATCTTAACTAACTATGTTACCGCTAAATTTGGCGCAAGCGGCATGG
TGTTATTGTCATCTGTGGTGACACTTGCCTGTTTAACCACTGCAGTTGGCCTTATCAGTGCCTGTGGTGAGTACTTTAAC
GAGCTACTGCCAAAGATCTCTTACCGTCGTTTTGTGGTGGGGGTAAGCATTGTTTGTGCAACTGTTGCTAACGTAGGTTT
GGCGCAGTTAATCGACATTAGCGTACCTGTTCTGATGACTGTTTACCCTGTTGCGATTGCATTGGTTGCGGTGACATTCT
TGACTGAGCGTTTTGCTAACCCGAAAGCGGCGCACCGTATCGTACTAAGCGTTGCGTTATTCTTCGGTATCATCGATGGC
TTTAGAGCCGCTAAGGTTGATGTAAGCGTATTTAACTTTATGCCACTTCATAATGAAGGCATGGCATGGTTACTGCCGAC
TGCTATCGCCATCGTGGCGTGCATGTTTATTAAAAAGCCTGCCGAGCAGCTAGCGATTAACTAA

Upstream 100 bases:

>100_bases
GGAGTCGTGTAAACTAATCATTACACCTTTTTATTGTCATCCTAAATGTGATGCCTTAGCGGCATGATGGCATTAAATAG
TTAACTAAAATAAAATCTAG

Downstream 100 bases:

>100_bases
AATCGCTTATGTGAATAGCTAAAGCAGATTGTCGAGTGGCGTATATAGCGCAAAATTGCTCGATGACATAATATTGAGGC
GATGCTTATAAAGCATCGCC

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGFLAGENITLAMFGFLITAVGLPLLTLIAVAKANGKIMQLLP
PIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLTDTSATFMIAGLAFNLTQLIYTLVFFGLSMVLALFPGKLLDSV
GKVLTPILILLLVGLAISVIVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTNY
LIKAALIAAAGLAFVYISLFYLGATAGDLATGAENGGVILTNYVTAKFGASGMVLLSSVVTLACLTTAVGLISACGEYFN
ELLPKISYRRFVVGVSIVCATVANVGLAQLIDISVPVLMTVYPVAIALVAVTFLTERFANPKAAHRIVLSVALFFGIIDG
FRAAKVDVSVFNFMPLHNEGMAWLLPTAIAIVACMFIKKPAEQLAIN

Sequences:

>Translated_447_residues
MGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGFLAGENITLAMFGFLITAVGLPLLTLIAVAKANGKIMQLLP
PIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLTDTSATFMIAGLAFNLTQLIYTLVFFGLSMVLALFPGKLLDSV
GKVLTPILILLLVGLAISVIVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTNY
LIKAALIAAAGLAFVYISLFYLGATAGDLATGAENGGVILTNYVTAKFGASGMVLLSSVVTLACLTTAVGLISACGEYFN
ELLPKISYRRFVVGVSIVCATVANVGLAQLIDISVPVLMTVYPVAIALVAVTFLTERFANPKAAHRIVLSVALFFGIIDG
FRAAKVDVSVFNFMPLHNEGMAWLLPTAIAIVACMFIKKPAEQLAIN
>Mature_446_residues
GHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGFLAGENITLAMFGFLITAVGLPLLTLIAVAKANGKIMQLLPP
IAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLTDTSATFMIAGLAFNLTQLIYTLVFFGLSMVLALFPGKLLDSVG
KVLTPILILLLVGLAISVIVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTNYL
IKAALIAAAGLAFVYISLFYLGATAGDLATGAENGGVILTNYVTAKFGASGMVLLSSVVTLACLTTAVGLISACGEYFNE
LLPKISYRRFVVGVSIVCATVANVGLAQLIDISVPVLMTVYPVAIALVAVTFLTERFANPKAAHRIVLSVALFFGIIDGF
RAAKVDVSVFNFMPLHNEGMAWLLPTAIAIVACMFIKKPAEQLAIN

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=443, Percent_Identity=41.3092550790068, Blast_Score=332, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46926; Mature: 46795

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGFLAGENITLAMFGFLITAVGL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PLLTLIAVAKANGKIMQLLPPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLTDT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCC
SATFMIAGLAFNLTQLIYTLVFFGLSMVLALFPGKLLDSVGKVLTPILILLLVGLAISVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
VIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTNY
EECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LIKAALIAAAGLAFVYISLFYLGATAGDLATGAENGGVILTNYVTAKFGASGMVLLSSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHH
TLACLTTAVGLISACGEYFNELLPKISYRRFVVGVSIVCATVANVGLAQLIDISVPVLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VYPVAIALVAVTFLTERFANPKAAHRIVLSVALFFGIIDGFRAAKVDVSVFNFMPLHNEG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHEECCCCCC
MAWLLPTAIAIVACMFIKKPAEQLAIN
CHHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
GHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGFLAGENITLAMFGFLITAVGL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PLLTLIAVAKANGKIMQLLPPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLTDT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCC
SATFMIAGLAFNLTQLIYTLVFFGLSMVLALFPGKLLDSVGKVLTPILILLLVGLAISVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
VIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTNY
EECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LIKAALIAAAGLAFVYISLFYLGATAGDLATGAENGGVILTNYVTAKFGASGMVLLSSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHH
TLACLTTAVGLISACGEYFNELLPKISYRRFVVGVSIVCATVANVGLAQLIDISVPVLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VYPVAIALVAVTFLTERFANPKAAHRIVLSVALFFGIIDGFRAAKVDVSVFNFMPLHNEG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHHHEECCCCCC
MAWLLPTAIAIVACMFIKKPAEQLAIN
CHHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]