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The map label for this gene is metK

Identifier: 167622783

GI number: 167622783

Start: 1047456

End: 1048610

Strand: Reverse

Name: metK

Synonym: Shal_0843

Alternate gene names: 167622783

Gene position: 1048610-1047456 (Counterclockwise)

Preceding gene: 167622792

Following gene: 167622780

Centisome position: 20.06

GC content: 47.71

Gene sequence:

>1155_bases
ATGGCAAAGCACTTGTTCACCTCTGAGTCGGTCTCAGAAGGTCATCCAGATAAAATCGCCGATCAGATTTCTGATGCGGT
ATTAGACGCAATTTTGGAGCAAGATCCAAAAGCTCGAGTAGCATGCGAAACCTATGTTAAAACTGGCATGGTTATGGTTG
GCGGCGAAGTTACCACTTCTGCTTGGGTTGATATCGAAGAGATCACCCGTAAGACTGTACGTGACATCGGCTACACTCAC
TCAGATATGGGTTTTGATGCTGACTCTTGTGCGATACTAAATGTTATCGGTAAGCAGTCTCCAGACATCAACCAAGGTGT
TGATCGTGCTGACCCTAAAGAGCAAGGCGCTGGTGACCAAGGTCTAATGTTTGGTTATGCCAACAATGAAACCGACTCGC
TAATGCCTGCGCCTATCACTTATTCGCACATGCTAGTTAAGCGTCAGTCTGAAGTTCGTAAAGACAAGACACTTCCTTGG
TTACGTCCAGATGCGAAGAGTCAGGTGACTTTTGCTTATAACCCAGATGGCAGCATTGCGGGTATCGACGCAGTGGTACT
TTCAACTCAGCATTGTGACAGCGTAAGCCAGTCAGATCTGATTGAAGGTGTAATGGAAACCATCATCAAGCCAGTATTAC
CGGCTAAGTGGTTATCTAAAGACACTAAATACTTCATCAACCCAACTGGCCGTTTTGTTATCGGTGGCCCAGTAGGCGAT
TGTGGTCTAACGGGTCGTAAGATTATCGTTGACACATACGGCGGTATGGCACGTCACGGTGGTGGTGCTTTCTCTGGTAA
AGACCCATCAAAAGTTGACCGTAGTGCAGCATACGCAGCACGTTACGTTGCTAAGAACATCGTTGCAGCCGGTCTAGCTG
ACCGTTGTGAGATCCAAGTGTCTTACGCAATTGGTGTGGCAGAGCCAACGTCAATCAGCATCGAAACATTTGGCACGGCT
AAAGTTGGCGAAGAGCTATTGATTGACCTAGTACGTCGTCACTTCGACCTACGTCCATACGGTCTAACAGAGATGTTAAA
CCTAGCTCGCCCAATCTACCAAGCGACTGCAGCATACGGTCACTTCGGTCGTGAAGAGTTCCCATGGGAAGCGACAGACA
AAGTTGAAGTGCTACGCGCTGACGCTGGTATTTAA

Upstream 100 bases:

>100_bases
TAGTGAAAGGGGTGTCATCGGCTCAGATTTACACTAGAATAGCCGTCTAGATGTAGATACTTCTACACATTCAAATTGTT
CAACGACGAGATTTTCCATC

Downstream 100 bases:

>100_bases
TCTTCATTGATTAACTCAGCGCTGTTTATTCAGCGCTAATAAAAAAACCGCCCAAGGGCGGTTTTTTTGTGTCTGGAACA
CTTATGACGATTTACCATGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRKTVRDIGYTH
SDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQGLMFGYANNETDSLMPAPITYSHMLVKRQSEVRKDKTLPW
LRPDAKSQVTFAYNPDGSIAGIDAVVLSTQHCDSVSQSDLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTA
KVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYGHFGREEFPWEATDKVEVLRADAGI

Sequences:

>Translated_384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRKTVRDIGYTH
SDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQGLMFGYANNETDSLMPAPITYSHMLVKRQSEVRKDKTLPW
LRPDAKSQVTFAYNPDGSIAGIDAVVLSTQHCDSVSQSDLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTA
KVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYGHFGREEFPWEATDKVEVLRADAGI
>Mature_383_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRKTVRDIGYTHS
DMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQGLMFGYANNETDSLMPAPITYSHMLVKRQSEVRKDKTLPWL
RPDAKSQVTFAYNPDGSIAGIDAVVLSTQHCDSVSQSDLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTAK
VGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYGHFGREEFPWEATDKVEVLRADAGI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=56.763925729443, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=56.9148936170213, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=83.0729166666667, Blast_Score=666, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=379, Percent_Identity=54.089709762533, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=53.4883720930233, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538494, Length=379, Percent_Identity=53.5620052770449, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=380, Percent_Identity=52.6315789473684, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565905, Length=379, Percent_Identity=48.5488126649077, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI32565911, Length=332, Percent_Identity=50.3012048192771, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=49.7058823529412, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=305, Percent_Identity=50.1639344262295, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=62.6506024096386, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=73.9130434782609, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=55.8510638297872, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=56.4864864864865, Blast_Score=406, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=53.5433070866142, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=53.5433070866142, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=53.5433070866142, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=53.5433070866142, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=53.5433070866142, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=53.5433070866142, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=52.8947368421053, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=52.8947368421053, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=379, Percent_Identity=50.6596306068602, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580539, Length=141, Percent_Identity=49.645390070922, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_SHEHH (B0TUB8)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673077.1
- ProteinModelPortal:   B0TUB8
- SMR:   B0TUB8
- GeneID:   5903058
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0843
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   SHAL458817:SHAL_0843-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41640; Mature: 41508

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRKTVRDIGYTHSDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQ
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYANNETDSLMPAPITYSHMLVKRQSEVRKDKTLPWLRPDAKSQVTFAYNPDGSIA
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
GIDAVVLSTQHCDSVSQSDLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CCHHEEEECHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQV
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISIETFGTAKVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYG
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
HFGREEFPWEATDKVEVLRADAGI
CCCCCCCCCCCCCHHHEEECCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRKTVRDIGYTHSDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQ
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYANNETDSLMPAPITYSHMLVKRQSEVRKDKTLPWLRPDAKSQVTFAYNPDGSIA
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
GIDAVVLSTQHCDSVSQSDLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CCHHEEEECHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQV
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISIETFGTAKVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYG
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
HFGREEFPWEATDKVEVLRADAGI
CCCCCCCCCCCCCHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA