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The map label for this gene is gluQ [H]

Identifier: 167622693

GI number: 167622693

Start: 933655

End: 934533

Strand: Reverse

Name: gluQ [H]

Synonym: Shal_0753

Alternate gene names: 167622693

Gene position: 934533-933655 (Counterclockwise)

Preceding gene: 167622694

Following gene: 167622692

Centisome position: 17.88

GC content: 47.33

Gene sequence:

>879_bases
ATGGTTAATACGACTCCCTATATAGGCCGCTTTGCGCCTTCCCCATCTGGTCCTCTTCATTTTGGTTCATTGGTTGCTGC
CCTCGGCAGTTTTTTGCGTGCCCGCTCCATGGGCGGAAAATGGTTGGTTCGTATCGAAGATATCGACCCGCCACGAGAGA
TCGCAGGCGCAAGCAGTGCTATTTTGAAAACCCTTGAAGCCTATGGCTTAGAGTGGGATGACAGCGTGCTCTATCAGAGC
CAACGCCTTGACGTATATCAGGCTAAAATTGATCAGTTACTATCTGATAACCAAGCTTATTATTGTCAATGTACGCGCAA
ACAGATCCAGACCATGGGCGGAAGCTACGATGGCCGATGTGGCGCACTATTACAGCCACTGACCCAAGGCGCAATTAGAG
TTCGTAACCCGCACGCAATCGATCATTTTGTCGACCTGCATATGGGACGAGTATCTGTAGATAAAGGCTTTGCTGCAGAA
GACTTTATTATCAAGCGTAGCGATGGGTTATATGCTTATCAGCTCGCTGTCGTGCTCGATGATATTCATCAGCAAATTAG
CGAAGTGGTAAGAGGATGCGATCTGCTTGAGCCAACTTGTCGTCAAGAAAGCTTATATCAAATCCTAAAAGCACCCGTGC
CCAAGTGGCTACACTTGCCATTGGCCTGTGCAGAAAAAGGCAGAAAACTGTCTAAGCAAAACCATGCGGCTGCAATCGAT
AATTGTCACCCGCAAGCCAGTATTAATGCGGCCTTAACTTTCTTAGGCCAACGTTCGGTGACTATGGAGTCTCATGTCAG
TAAGATGATTGAACAGGCCGTGAGTCAATTTGATTTGACCAAGGTGCCAAAACAATCGGAAATACTGCTTGATACGTGA

Upstream 100 bases:

>100_bases
CTGTAAAACGCTTGCTGAGATCAAAGAAAAGCAAATGGCAGGTTAATTTCTGCCTAAAGTGCGGAGCTTAGGCTCCGCTC
TTTTGTTTTATTATCAGTAA

Downstream 100 bases:

>100_bases
GATCAATCGTATACTCATTAAGCTTTAATTGAGTTATCATAGCCCACTTGTTTTTACCCAAATTAAGATCCAAATCAGAG
GTGTCCCATTTTTCGCCGTA

Product: glutamate--tRNA ligase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVNTTPYIGRFAPSPSGPLHFGSLVAALGSFLRARSMGGKWLVRIEDIDPPREIAGASSAILKTLEAYGLEWDDSVLYQS
QRLDVYQAKIDQLLSDNQAYYCQCTRKQIQTMGGSYDGRCGALLQPLTQGAIRVRNPHAIDHFVDLHMGRVSVDKGFAAE
DFIIKRSDGLYAYQLAVVLDDIHQQISEVVRGCDLLEPTCRQESLYQILKAPVPKWLHLPLACAEKGRKLSKQNHAAAID
NCHPQASINAALTFLGQRSVTMESHVSKMIEQAVSQFDLTKVPKQSEILLDT

Sequences:

>Translated_292_residues
MVNTTPYIGRFAPSPSGPLHFGSLVAALGSFLRARSMGGKWLVRIEDIDPPREIAGASSAILKTLEAYGLEWDDSVLYQS
QRLDVYQAKIDQLLSDNQAYYCQCTRKQIQTMGGSYDGRCGALLQPLTQGAIRVRNPHAIDHFVDLHMGRVSVDKGFAAE
DFIIKRSDGLYAYQLAVVLDDIHQQISEVVRGCDLLEPTCRQESLYQILKAPVPKWLHLPLACAEKGRKLSKQNHAAAID
NCHPQASINAALTFLGQRSVTMESHVSKMIEQAVSQFDLTKVPKQSEILLDT
>Mature_292_residues
MVNTTPYIGRFAPSPSGPLHFGSLVAALGSFLRARSMGGKWLVRIEDIDPPREIAGASSAILKTLEAYGLEWDDSVLYQS
QRLDVYQAKIDQLLSDNQAYYCQCTRKQIQTMGGSYDGRCGALLQPLTQGAIRVRNPHAIDHFVDLHMGRVSVDKGFAAE
DFIIKRSDGLYAYQLAVVLDDIHQQISEVVRGCDLLEPTCRQESLYQILKAPVPKWLHLPLACAEKGRKLSKQNHAAAID
NCHPQASINAALTFLGQRSVTMESHVSKMIEQAVSQFDLTKVPKQSEILLDT

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=311, Percent_Identity=32.7974276527331, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI226510925, Length=290, Percent_Identity=52.0689655172414, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1788743, Length=297, Percent_Identity=34.006734006734, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71993394, Length=315, Percent_Identity=30.4761904761905, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324540, Length=315, Percent_Identity=31.7460317460317, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24665321, Length=274, Percent_Identity=32.8467153284672, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 32367; Mature: 32367

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTTPYIGRFAPSPSGPLHFGSLVAALGSFLRARSMGGKWLVRIEDIDPPREIAGASSA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHCCHHH
ILKTLEAYGLEWDDSVLYQSQRLDVYQAKIDQLLSDNQAYYCQCTRKQIQTMGGSYDGRC
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCH
GALLQPLTQGAIRVRNPHAIDHFVDLHMGRVSVDKGFAAEDFIIKRSDGLYAYQLAVVLD
HHHHHHHHHCCEEECCCCHHHHHHHHHCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHH
DIHQQISEVVRGCDLLEPTCRQESLYQILKAPVPKWLHLPLACAEKGRKLSKQNHAAAID
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHHCHHHHHHCCCHHHC
NCHPQASINAALTFLGQRSVTMESHVSKMIEQAVSQFDLTKVPKQSEILLDT
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure
MVNTTPYIGRFAPSPSGPLHFGSLVAALGSFLRARSMGGKWLVRIEDIDPPREIAGASSA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHCCHHH
ILKTLEAYGLEWDDSVLYQSQRLDVYQAKIDQLLSDNQAYYCQCTRKQIQTMGGSYDGRC
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCH
GALLQPLTQGAIRVRNPHAIDHFVDLHMGRVSVDKGFAAEDFIIKRSDGLYAYQLAVVLD
HHHHHHHHHCCEEECCCCHHHHHHHHHCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHH
DIHQQISEVVRGCDLLEPTCRQESLYQILKAPVPKWLHLPLACAEKGRKLSKQNHAAAID
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHHCHHHHHHCCCHHHC
NCHPQASINAALTFLGQRSVTMESHVSKMIEQAVSQFDLTKVPKQSEILLDT
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]