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The map label for this gene is pcnB [H]

Identifier: 167622692

GI number: 167622692

Start: 932182

End: 933474

Strand: Reverse

Name: pcnB [H]

Synonym: Shal_0752

Alternate gene names: 167622692

Gene position: 933474-932182 (Counterclockwise)

Preceding gene: 167622693

Following gene: 167622691

Centisome position: 17.86

GC content: 48.41

Gene sequence:

>1293_bases
GTGCCTAGAAATGCGCACGCTATTTCTCGCAGACAGATCAGTGACAATGCGATCAAAGTACTCTATAGACTGCACAAGTC
AGGCTTCAAAGCCTACTTGGTAGGCGGTGGTGTACGCGATATTTTATTGGGCTTACAACCGAAAGACTTCGACGTTGTTA
CCAATGCCACCCCTGAAGAGATTAAACGACTATTCCGTAACTGCCGTTTAGTTGGTCGTCGCTTCCGCCTCGCTCATATC
GTTTTTGGGCGCGATGTGATTGAAGTTGCGACTTTACGCGGCCATCACGTAGATAGCGAAGAGAAGATCTCTAAGACCAA
TGAGTCTGGCCGTCTATTGCGCGACAACGTCTACGGCAGTATCGATGAAGACGCCGAGCGTCGTGACTTTACCGTTAACG
CCCTATATTACGACATCAGTGATTTCTCTATTCATAGCTACGGCGGTGGGTTACAAGATCTAGAGTCTCGTACAATCCGT
TTAATTGGCGACCCAGAGACTCGCTATCGTGAAGATCCTGTGCGTATGCTACGTGCAGTGCGTTTCGCGACTAAGCTGGA
TATGACCATTGAAGCGAATGCCGCAAAACCGATTAAAGGTTTAGCGCGATTGCTTAAAGATATTCCTGCTGCTCGTATGT
ATGAAGAAGTACTGAAGCTGTTTTTCGCCGGGAAAGCACTTGAAAACTTCCATATGATGCGCGAGTTTGGACTATTTGAA
CCGCTATTCCCTCAGGTTGAAGCACTGTTAAAAGAATATCCAAGCGGTGAAACCGCTAAGTTATTGCAAGAGATGATGAC
AAGTACCGATGAGCGCGTCGCACAAGACAAGCCGGTTACCCCAGCATTTTTCTATGCAGCGCTGCTTTGGTACCCACTAA
GTCAGCGTGCTGACGATATCGCCCTAGAAAGCGGTCTATCAACCTATGACGCTTACACTGCGGCCATGGGTGATGTCATG
GAGCAGCAATGCCGCACCATTAGCATACCGCGCCGCTTTAGCACTGTGACCAAAGATATTTGGCAGCTACAACTTAGGTT
CGAACGTAACAAGGGCACCAAGGCATTTAGATTCATTGAGCATCCTAAGTTTAGAGCCGCTTATGATCTACTCTTGCTAC
GTGGCCATGCCGAAGGTGGTGCTACCGCGAAGAATGCCGCATGGTGGAAGAGCTTTGTTGAAGGCAATGAAGATCAGCGT
AATGTGATTGCACGCTCAGCCAATAAAGGGGGTCGTAACCGTAACACTAATCAACGTCGACGCCGCAAGCCTAGCACCAA
ACCTGCAGAATAA

Upstream 100 bases:

>100_bases
GTGTCCCATTTTTCGCCGTATTAGCCAATTCTGTAAACAGCTATTTGAAGATACTCCAAAAGCTGAAGAAAACACGCAGA
CAGGTCTTAGTTTAGAGGTC

Downstream 100 bases:

>100_bases
TGGCTCAAGTCTACGTTGCGTTAGGCGCAAACTTAGCCGATCCGATAAGCCAGCTCAATAGCGCTTGTGAAGCCTTATTG
AGCTTGGCAACTGTGGACTC

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPRNAHAISRRQISDNAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEEIKRLFRNCRLVGRRFRLAHI
VFGRDVIEVATLRGHHVDSEEKISKTNESGRLLRDNVYGSIDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIR
LIGDPETRYREDPVRMLRAVRFATKLDMTIEANAAKPIKGLARLLKDIPAARMYEEVLKLFFAGKALENFHMMREFGLFE
PLFPQVEALLKEYPSGETAKLLQEMMTSTDERVAQDKPVTPAFFYAALLWYPLSQRADDIALESGLSTYDAYTAAMGDVM
EQQCRTISIPRRFSTVTKDIWQLQLRFERNKGTKAFRFIEHPKFRAAYDLLLLRGHAEGGATAKNAAWWKSFVEGNEDQR
NVIARSANKGGRNRNTNQRRRRKPSTKPAE

Sequences:

>Translated_430_residues
MPRNAHAISRRQISDNAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEEIKRLFRNCRLVGRRFRLAHI
VFGRDVIEVATLRGHHVDSEEKISKTNESGRLLRDNVYGSIDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIR
LIGDPETRYREDPVRMLRAVRFATKLDMTIEANAAKPIKGLARLLKDIPAARMYEEVLKLFFAGKALENFHMMREFGLFE
PLFPQVEALLKEYPSGETAKLLQEMMTSTDERVAQDKPVTPAFFYAALLWYPLSQRADDIALESGLSTYDAYTAAMGDVM
EQQCRTISIPRRFSTVTKDIWQLQLRFERNKGTKAFRFIEHPKFRAAYDLLLLRGHAEGGATAKNAAWWKSFVEGNEDQR
NVIARSANKGGRNRNTNQRRRRKPSTKPAE
>Mature_429_residues
PRNAHAISRRQISDNAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEEIKRLFRNCRLVGRRFRLAHIV
FGRDVIEVATLRGHHVDSEEKISKTNESGRLLRDNVYGSIDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIRL
IGDPETRYREDPVRMLRAVRFATKLDMTIEANAAKPIKGLARLLKDIPAARMYEEVLKLFFAGKALENFHMMREFGLFEP
LFPQVEALLKEYPSGETAKLLQEMMTSTDERVAQDKPVTPAFFYAALLWYPLSQRADDIALESGLSTYDAYTAAMGDVME
QQCRTISIPRRFSTVTKDIWQLQLRFERNKGTKAFRFIEHPKFRAAYDLLLLRGHAEGGATAKNAAWWKSFVEGNEDQRN
VIARSANKGGRNRNTNQRRRRKPSTKPAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=234, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI87081691, Length=407, Percent_Identity=54.5454545454545, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=30.7017543859649, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=225, Percent_Identity=29.7777777777778, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=183, Percent_Identity=27.8688524590164, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281360112, Length=277, Percent_Identity=27.4368231046931, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357337, Length=277, Percent_Identity=27.4368231046931, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 49211; Mature: 49080

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNAHAISRRQISDNAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCEECCCCHHH
IKRLFRNCRLVGRRFRLAHIVFGRDVIEVATLRGHHVDSEEKISKTNESGRLLRDNVYGS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCCCCCEEHHCCCCC
IDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHCCCEEEEECCCHHHHCCCHHHHHHHH
RFATKLDMTIEANAAKPIKGLARLLKDIPAARMYEEVLKLFFAGKALENFHMMREFGLFE
HHHHHHCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLFPQVEALLKEYPSGETAKLLQEMMTSTDERVAQDKPVTPAFFYAALLWYPLSQRADDI
CHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHH
ALESGLSTYDAYTAAMGDVMEQQCRTISIPRRFSTVTKDIWQLQLRFERNKGTKAFRFIE
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHC
HPKFRAAYDLLLLRGHAEGGATAKNAAWWKSFVEGNEDQRNVIARSANKGGRNRNTNQRR
CCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHH
RRKPSTKPAE
HCCCCCCCCC
>Mature Secondary Structure 
PRNAHAISRRQISDNAIKVLYRLHKSGFKAYLVGGGVRDILLGLQPKDFDVVTNATPEE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCEECCCCHHH
IKRLFRNCRLVGRRFRLAHIVFGRDVIEVATLRGHHVDSEEKISKTNESGRLLRDNVYGS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCCCCCEEHHCCCCC
IDEDAERRDFTVNALYYDISDFSIHSYGGGLQDLESRTIRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHCCCEEEEECCCHHHHCCCHHHHHHHH
RFATKLDMTIEANAAKPIKGLARLLKDIPAARMYEEVLKLFFAGKALENFHMMREFGLFE
HHHHHHCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLFPQVEALLKEYPSGETAKLLQEMMTSTDERVAQDKPVTPAFFYAALLWYPLSQRADDI
CHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHH
ALESGLSTYDAYTAAMGDVMEQQCRTISIPRRFSTVTKDIWQLQLRFERNKGTKAFRFIE
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHC
HPKFRAAYDLLLLRGHAEGGATAKNAAWWKSFVEGNEDQRNVIARSANKGGRNRNTNQRR
CCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHH
RRKPSTKPAE
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]