Click here to switch to the map view.

The map label for this gene is dcuB [H]

Identifier: 167622682

GI number: 167622682

Start: 922752

End: 924068

Strand: Reverse

Name: dcuB [H]

Synonym: Shal_0742

Alternate gene names: 167622682

Gene position: 924068-922752 (Counterclockwise)

Preceding gene: 167622688

Following gene: 167622681

Centisome position: 17.68

GC content: 46.92

Gene sequence:

>1317_bases
ATGTTCTTCATACATATGCTCTTATTATTAACTGTTATTTTTGTAGGGATCCGCCATGGTGGTGTGGCCTTTGGTCTACT
CGGTGGCTTAGGGGTATCGATATTAGCCTTTGTATTTGGCATTGCCCCCGGTACTCCCCCTATCAACGTGATGTTGATTA
TTTTAGCGGTAGTCGCTGCATCGGCGACACTAGAGGCGACGGGCGGCCTTAAATTACTGGTGCGTTATGCAGAAAAGCTG
CTGCGTAAACACCCAAACCAAATCGTATTTCTCGGTCCATTGTGTACTTACTCACTCACCGTTTTGGTCGGTACCGGTCA
CTCTGTTTATCCTCTACTTCCGGTTATCTATGATGTTGCCTATAAGAAAGGCATTCGCCCTGAACGACCATTAGCTGTGG
CGACAGTCGCCTCACAGATGGGGATTACCGCCAGCCCTATTGCAGCTGCGGCGGCTGTAGTGATTGCCACCTCAATGGAG
AATAATCTCGATATCAGCTTAGTGGACGTACTCCTAGTCACGATTCCATCGACATTAATCGGTGTGTTAGTCGCGGCCAC
TTGGAGCCTTAATCGCGGTAAAGATCTCGATAAAGACCCAGAATTTCAAGCTCGTCTGCAAGATGAAGACTTTAGAAACA
GCCTAGTTGATACCGAAATTGAATCTAGCGATGTGGCGAAATCAGACAGTACCGCCAAGAAAGGCCTAACCATTTTCCTA
CTGGGTATACTATCTGTGATTGCCTTAGCGATATTTAGCGACCACTTGCTACCTGAAGGCGTGAAAATGTCGGTAGCGAT
TCAGTTTATGATGCTATCTGTGGGTGCGGTTATCTTGCTTGCAACCAAGATAGACCCGAAAAAAATCGTCCACAGCAATG
TGTTTATCGCTGGTATGACTGCGGTGATCATTATCTTCGGCATCGCTTGGCTAAGTGACACCATTATTGGTCACCACAAG
GCTTACTTAATCGAGTCGGTAAGCGATATCGTCCATGCCCATCCGTGGTCATTTGCCATCGCCATGTTTACCGCCTCGGT
ATTCCTTAAGAGCCAAGCTGCAACCCTGACGATCATGCTACCACTTGGGTTCTCTTTAGGTATTCCAGCCCCAGTGCTTA
TCGGTGTCTTGCCTGCTTGTTACGCCTATTTCTTCTTCCCATTTTATCCAAGCGATCTGGCGGCCATCAGCTTTGACAGA
ACTGGCACCACTCATATCGGTAAGTATATAGTCAATCACAGCTTTATCATGCCGGGATTTATCGGGGTTATTACAGCAAC
CGTGATAGGCTATTTCATCTCTATGATGGTCAATTAG

Upstream 100 bases:

>100_bases
TAACATACGGTATAACCAGCTTTTAGAGGCCGACATCATGGACAGTGTCTATGTCAGCACTCTGTCAACGAGTCGCTTAT
CCGCTTAAAGGAACCCCGCT

Downstream 100 bases:

>100_bases
TTTCAAGTTTACCTACCGCTGATTTAGCTGCATATTACAGCTAATACGGATATGCTTTAGGCGCCCTAATGGGTGCCTTT
TTTATGCTTGCCCTTTCACG

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSILAFVFGIAPGTPPINVMLIILAVVAASATLEATGGLKLLVRYAEKL
LRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVAYKKGIRPERPLAVATVASQMGITASPIAAAAAVVIATSME
NNLDISLVDVLLVTIPSTLIGVLVAATWSLNRGKDLDKDPEFQARLQDEDFRNSLVDTEIESSDVAKSDSTAKKGLTIFL
LGILSVIALAIFSDHLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMTAVIIIFGIAWLSDTIIGHHK
AYLIESVSDIVHAHPWSFAIAMFTASVFLKSQAATLTIMLPLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDR
TGTTHIGKYIVNHSFIMPGFIGVITATVIGYFISMMVN

Sequences:

>Translated_438_residues
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSILAFVFGIAPGTPPINVMLIILAVVAASATLEATGGLKLLVRYAEKL
LRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVAYKKGIRPERPLAVATVASQMGITASPIAAAAAVVIATSME
NNLDISLVDVLLVTIPSTLIGVLVAATWSLNRGKDLDKDPEFQARLQDEDFRNSLVDTEIESSDVAKSDSTAKKGLTIFL
LGILSVIALAIFSDHLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMTAVIIIFGIAWLSDTIIGHHK
AYLIESVSDIVHAHPWSFAIAMFTASVFLKSQAATLTIMLPLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDR
TGTTHIGKYIVNHSFIMPGFIGVITATVIGYFISMMVN
>Mature_438_residues
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSILAFVFGIAPGTPPINVMLIILAVVAASATLEATGGLKLLVRYAEKL
LRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVAYKKGIRPERPLAVATVASQMGITASPIAAAAAVVIATSME
NNLDISLVDVLLVTIPSTLIGVLVAATWSLNRGKDLDKDPEFQARLQDEDFRNSLVDTEIESSDVAKSDSTAKKGLTIFL
LGILSVIALAIFSDHLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMTAVIIIFGIAWLSDTIIGHHK
AYLIESVSDIVHAHPWSFAIAMFTASVFLKSQAATLTIMLPLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDR
TGTTHIGKYIVNHSFIMPGFIGVITATVIGYFISMMVN

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=409, Percent_Identity=42.5427872860636, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1790580, Length=436, Percent_Identity=38.7614678899083, Blast_Score=261, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46812; Mature: 46812

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSILAFVFGIAPGTPPINVMLIILAVVAA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SATLEATGGLKLLVRYAEKLLRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVA
CCCEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHEEEEEEEECCCCHHHHHHHHHHHH
YKKGIRPERPLAVATVASQMGITASPIAAAAAVVIATSMENNLDISLVDVLLVTIPSTLI
HHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GVLVAATWSLNRGKDLDKDPEFQARLQDEDFRNSLVDTEIESSDVAKSDSTAKKGLTIFL
HHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHH
LGILSVIALAIFSDHLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHH
AVIIIFGIAWLSDTIIGHHKAYLIESVSDIVHAHPWSFAIAMFTASVFLKSQAATLTIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEE
PLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDRTGTTHIGKYIVNHSFIMPGF
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHCCCCHHHHH
IGVITATVIGYFISMMVN
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSILAFVFGIAPGTPPINVMLIILAVVAA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SATLEATGGLKLLVRYAEKLLRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVA
CCCEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHEEEEEEEECCCCHHHHHHHHHHHH
YKKGIRPERPLAVATVASQMGITASPIAAAAAVVIATSMENNLDISLVDVLLVTIPSTLI
HHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GVLVAATWSLNRGKDLDKDPEFQARLQDEDFRNSLVDTEIESSDVAKSDSTAKKGLTIFL
HHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHH
LGILSVIALAIFSDHLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHH
AVIIIFGIAWLSDTIIGHHKAYLIESVSDIVHAHPWSFAIAMFTASVFLKSQAATLTIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEE
PLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDRTGTTHIGKYIVNHSFIMPGF
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHCCCCHHHHH
IGVITATVIGYFISMMVN
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]