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The map label for this gene is ygfZ [C]

Identifier: 167622662

GI number: 167622662

Start: 897471

End: 898442

Strand: Reverse

Name: ygfZ [C]

Synonym: Shal_0722

Alternate gene names: 167622662

Gene position: 898442-897471 (Counterclockwise)

Preceding gene: 167622663

Following gene: 167622659

Centisome position: 17.19

GC content: 47.84

Gene sequence:

>972_bases
ATGACGCTGTCAACTTCTCAGCCAACTTGGCCTCTTAATGGACAACAGCCTGAACTGCTTTTTTCTGAATTAACACACCT
AGGTTTAATCTCAGTCACTGGGGAGCAAGGTCGCAGCTTTATTCACGGCCAAGTCACTACCGATATCAGCTCGCTTGAAA
GCGACCAATGGCGCTGGGGCGCGCATTGCGATCCTAAAGGTAAGATGCTGGCCAGCTTTAGAACCTTCGCCAAAGGTGAC
ACCCTTTTCTTGATGATGCCAAAAGAGACCTTGGCACTGGATCTACCTCAGTTGCAAAAATATGCGGTATTTAGTAAAGC
TGAGCTGTCTGATGCCAGTGATCACTGGTTATTGTTAGGCGTGGCGGGCGAACAAGCTAAAACATGGTTAACGGCTCAGT
TTGGCGAGCTTACCGATGAGCTAACCTTAATCGATAACGGCATGATCATCCACGATGCGGGTCGGTTTATCCTCGCGATA
GAACAAGACCAAGCCGCATCACTTATCAGTGCTATCGAACAACCTATCTATGATGCTACTGCATGGCAGGCACTGGAAAT
CGCTGCCGGTTATCCAAACCTAGGTGCTAATCATCAAGGACAGTTTGTACCGCAGATGTGTAATGTCCAAGCAGTCAATG
GGATCAGCTTCAATAAGGGCTGTTACATGGGACAAGAGACGATTGCTCGAATGAAGTACCGCGGGGGTAACAAGCGTGCG
CTTTACATTGTTAGCGGCACGGTATCAACACCTTTAACAGCCGATAGCCAGCTTGAAATTGCACTAGAAGACGGTGAAGG
ATTTCGCCGCGCAGGCACTATCATCGAGGCTGTTCAACGGGGTGACCAGGTATTATTAACAGCGGTATTAGCTAATGATA
CTCAACTTGATGCTAAGTTAAGAGTTGCCGATGATGCGAGCTCTGAACTGACATTAATTGCGCTGCCTTACTCGCTAGAA
GAGCAAGATTAA

Upstream 100 bases:

>100_bases
TATATATATTGAGTAGCAATAGTTTGAACTGCTGCGCTTGCACGGCAAGATTTTAGCGGTACAATCATTGTCCTTATTGG
TTTAATTCTTGGATCCTCAT

Downstream 100 bases:

>100_bases
TCCAGCCCCCAATTCGTTCGTAATAGAAAAGGCGCTTTATGCGCCTTTTTGTTTATCCCGACTAATCAAGACTAAATCTA
ACCCGTCTCATTGTGAGCAT

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTLSTSQPTWPLNGQQPELLFSELTHLGLISVTGEQGRSFIHGQVTTDISSLESDQWRWGAHCDPKGKMLASFRTFAKGD
TLFLMMPKETLALDLPQLQKYAVFSKAELSDASDHWLLLGVAGEQAKTWLTAQFGELTDELTLIDNGMIIHDAGRFILAI
EQDQAASLISAIEQPIYDATAWQALEIAAGYPNLGANHQGQFVPQMCNVQAVNGISFNKGCYMGQETIARMKYRGGNKRA
LYIVSGTVSTPLTADSQLEIALEDGEGFRRAGTIIEAVQRGDQVLLTAVLANDTQLDAKLRVADDASSELTLIALPYSLE
EQD

Sequences:

>Translated_323_residues
MTLSTSQPTWPLNGQQPELLFSELTHLGLISVTGEQGRSFIHGQVTTDISSLESDQWRWGAHCDPKGKMLASFRTFAKGD
TLFLMMPKETLALDLPQLQKYAVFSKAELSDASDHWLLLGVAGEQAKTWLTAQFGELTDELTLIDNGMIIHDAGRFILAI
EQDQAASLISAIEQPIYDATAWQALEIAAGYPNLGANHQGQFVPQMCNVQAVNGISFNKGCYMGQETIARMKYRGGNKRA
LYIVSGTVSTPLTADSQLEIALEDGEGFRRAGTIIEAVQRGDQVLLTAVLANDTQLDAKLRVADDASSELTLIALPYSLE
EQD
>Mature_322_residues
TLSTSQPTWPLNGQQPELLFSELTHLGLISVTGEQGRSFIHGQVTTDISSLESDQWRWGAHCDPKGKMLASFRTFAKGDT
LFLMMPKETLALDLPQLQKYAVFSKAELSDASDHWLLLGVAGEQAKTWLTAQFGELTDELTLIDNGMIIHDAGRFILAIE
QDQAASLISAIEQPIYDATAWQALEIAAGYPNLGANHQGQFVPQMCNVQAVNGISFNKGCYMGQETIARMKYRGGNKRAL
YIVSGTVSTPLTADSQLEIALEDGEGFRRAGTIIEAVQRGDQVLLTAVLANDTQLDAKLRVADDASSELTLIALPYSLEE
QD

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=330, Percent_Identity=39.0909090909091, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 35280; Mature: 35149

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSTSQPTWPLNGQQPELLFSELTHLGLISVTGEQGRSFIHGQVTTDISSLESDQWRWG
CCCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCEEEECEEECCHHHCCCCCCEEC
AHCDPKGKMLASFRTFAKGDTLFLMMPKETLALDLPQLQKYAVFSKAELSDASDHWLLLG
CCCCCCCHHHHHHHHHCCCCEEEEEECCHHHEECCHHHHHHHHHCCCCCCCCCCCEEEEE
VAGEQAKTWLTAQFGELTDELTLIDNGMIIHDAGRFILAIEQDQAASLISAIEQPIYDAT
ECCCCHHEEEEEHHHCCCCEEEEEECCEEEEECCCEEEEECCCHHHHHHHHHHHCCCCCH
AWQALEIAAGYPNLGANHQGQFVPQMCNVQAVNGISFNKGCYMGQETIARMKYRGGNKRA
HHHEEEEECCCCCCCCCCCCCCCHHHCCEEEECCEEECCCCCCCHHHHHHHHCCCCCCEE
LYIVSGTVSTPLTADSQLEIALEDGEGFRRAGTIIEAVQRGDQVLLTAVLANDTQLDAKL
EEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEE
RVADDASSELTLIALPYSLEEQD
EEECCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TLSTSQPTWPLNGQQPELLFSELTHLGLISVTGEQGRSFIHGQVTTDISSLESDQWRWG
CCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCEEEECEEECCHHHCCCCCCEEC
AHCDPKGKMLASFRTFAKGDTLFLMMPKETLALDLPQLQKYAVFSKAELSDASDHWLLLG
CCCCCCCHHHHHHHHHCCCCEEEEEECCHHHEECCHHHHHHHHHCCCCCCCCCCCEEEEE
VAGEQAKTWLTAQFGELTDELTLIDNGMIIHDAGRFILAIEQDQAASLISAIEQPIYDAT
ECCCCHHEEEEEHHHCCCCEEEEEECCEEEEECCCEEEEECCCHHHHHHHHHHHCCCCCH
AWQALEIAAGYPNLGANHQGQFVPQMCNVQAVNGISFNKGCYMGQETIARMKYRGGNKRA
HHHEEEEECCCCCCCCCCCCCCCHHHCCEEEECCEEECCCCCCCHHHHHHHHCCCCCCEE
LYIVSGTVSTPLTADSQLEIALEDGEGFRRAGTIIEAVQRGDQVLLTAVLANDTQLDAKL
EEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEE
RVADDASSELTLIALPYSLEEQD
EEECCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]