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The map label for this gene is yxeI [H]

Identifier: 167622531

GI number: 167622531

Start: 716675

End: 717793

Strand: Direct

Name: yxeI [H]

Synonym: Shal_0591

Alternate gene names: 167622531

Gene position: 716675-717793 (Clockwise)

Preceding gene: 167622530

Following gene: 167622532

Centisome position: 13.71

GC content: 45.4

Gene sequence:

>1119_bases
ATGAATACCAACCGGTATAAGATTGAAATCATGAAAAAATCACTACTCGCTTTACTTCTAACCTCTGCTTTCAGTTATCA
CGCAAGTGCCTGTACTGGCATTACCCTATCAACCACAGACAATGATCAGGTTCAGGCTCGCACCATAGAGTGGGGCCATA
GTGATCTCAACAGCAAGCTTGTTATCTCGCCTCGTAATCACCAATACACTTCGACTATGCCCGATCAGCAGCAAGGTCTC
ACCTGGAAGAGTCAATATGGCTTTGCTGGGATCTCGGTGAGTGACGATCGCTTTATTGCCGAGGGAATTAACGAGAAAGG
ACTGACTGCCGGGTTATTCTATTTTCGCAACTATGGCTCTCTGGCCAAGTATGACCCTAAGCAGACAGCAAACAACATTA
CCGATATGGACTTTGTGCGTTGGATGTTAACCCAGTTTGAAACCGTTGCCGAGGTCGAGGCGGCATTAGATAAAATTAAG
ATTGTCACCGTTTATTTTGATCAGCAAGGTAACGCCTCGCCGACGGCGCACTGGCGCGTGTCTGATAAGCAGGGCAATAG
CATTGTGATTGAAATCATGGATCACGGCAAAATCAATATTCATAAGAACACGGCTAAGGTGCTAACCAACTCGCCAGATT
ACAACTGGCAGGTGACCAACCTTAATAATTATATCAATCTGCATCCTGGGATCAGCCCACCTCAGAAGATTAATGGTGTA
GAGGCACAATCTTTTGGTGTTGGCTCTAACTTTGTTGGTTTACCGGGAGATATTTCACCGCCATCGCGATTCGTGCGTGC
AGCGTTTTACGTGAATACTGCGCCTAAGTTGAATACTGCCGAACAAGCGGTTTCACAGGCGTTCCATATCTTAAATAACT
TCGATATTCCCATTGGCAGTGAGTTTAACGATAAGTCTCATATCCCAGAGCTGCCCAGTGCAACGCAGTGGACCTCGGTT
ATCGATCAGAGTCATGGGTTACTGTTCTATAAGACCATGCATGACAGCACGATTAAGCGAGTCGATCTGAAGCAGTTAGA
TTTTACCGTTAAGCAGGAACGTAAACAGAAGTTAGACAGTGGTAAGTTTAGCTATCAAGAGGTAGGCGTAAGCAATTAA

Upstream 100 bases:

>100_bases
GACTTAAGATTTTGTCATTTTTGCTTTTCGGGTGTACCTTAACTTGTCGGATAGCAATCGTTCAAATAGGATTGGTTTTA
GCTATCTTCTTTTGGCTTAG

Downstream 100 bases:

>100_bases
TTATCGCGCTTGTCCCCACTTGATAAGAAGCCCTGACATAAATGCCAGGGCTTCTTGGTTTCTTGCTAATTATTTATAGC
CTTTGTTAGCTACAGAGTAA

Product: choloylglycine hydrolase

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNTNRYKIEIMKKSLLALLLTSAFSYHASACTGITLSTTDNDQVQARTIEWGHSDLNSKLVISPRNHQYTSTMPDQQQGL
TWKSQYGFAGISVSDDRFIAEGINEKGLTAGLFYFRNYGSLAKYDPKQTANNITDMDFVRWMLTQFETVAEVEAALDKIK
IVTVYFDQQGNASPTAHWRVSDKQGNSIVIEIMDHGKINIHKNTAKVLTNSPDYNWQVTNLNNYINLHPGISPPQKINGV
EAQSFGVGSNFVGLPGDISPPSRFVRAAFYVNTAPKLNTAEQAVSQAFHILNNFDIPIGSEFNDKSHIPELPSATQWTSV
IDQSHGLLFYKTMHDSTIKRVDLKQLDFTVKQERKQKLDSGKFSYQEVGVSN

Sequences:

>Translated_372_residues
MNTNRYKIEIMKKSLLALLLTSAFSYHASACTGITLSTTDNDQVQARTIEWGHSDLNSKLVISPRNHQYTSTMPDQQQGL
TWKSQYGFAGISVSDDRFIAEGINEKGLTAGLFYFRNYGSLAKYDPKQTANNITDMDFVRWMLTQFETVAEVEAALDKIK
IVTVYFDQQGNASPTAHWRVSDKQGNSIVIEIMDHGKINIHKNTAKVLTNSPDYNWQVTNLNNYINLHPGISPPQKINGV
EAQSFGVGSNFVGLPGDISPPSRFVRAAFYVNTAPKLNTAEQAVSQAFHILNNFDIPIGSEFNDKSHIPELPSATQWTSV
IDQSHGLLFYKTMHDSTIKRVDLKQLDFTVKQERKQKLDSGKFSYQEVGVSN
>Mature_372_residues
MNTNRYKIEIMKKSLLALLLTSAFSYHASACTGITLSTTDNDQVQARTIEWGHSDLNSKLVISPRNHQYTSTMPDQQQGL
TWKSQYGFAGISVSDDRFIAEGINEKGLTAGLFYFRNYGSLAKYDPKQTANNITDMDFVRWMLTQFETVAEVEAALDKIK
IVTVYFDQQGNASPTAHWRVSDKQGNSIVIEIMDHGKINIHKNTAKVLTNSPDYNWQVTNLNNYINLHPGISPPQKINGV
EAQSFGVGSNFVGLPGDISPPSRFVRAAFYVNTAPKLNTAEQAVSQAFHILNNFDIPIGSEFNDKSHIPELPSATQWTSV
IDQSHGLLFYKTMHDSTIKRVDLKQLDFTVKQERKQKLDSGKFSYQEVGVSN

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 41696; Mature: 41696

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNRYKIEIMKKSLLALLLTSAFSYHASACTGITLSTTDNDQVQARTIEWGHSDLNSKL
CCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCCCEEEEEEECCCCCCCCEE
VISPRNHQYTSTMPDQQQGLTWKSQYGFAGISVSDDRFIAEGINEKGLTAGLFYFRNYGS
EECCCCCCCCCCCCCHHHCCEEECCCCEEEEEECCCCHHHCCCCCCCCEEHHHHHHCCCC
LAKYDPKQTANNITDMDFVRWMLTQFETVAEVEAALDKIKIVTVYFDQQGNASPTAHWRV
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCEEEEE
SDKQGNSIVIEIMDHGKINIHKNTAKVLTNSPDYNWQVTNLNNYINLHPGISPPQKINGV
ECCCCCEEEEEEECCCEEEEECCHHEEEECCCCCCEEEEECCCEEEECCCCCCHHHCCCC
EAQSFGVGSNFVGLPGDISPPSRFVRAAFYVNTAPKLNTAEQAVSQAFHILNNFDIPIGS
CCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
EFNDKSHIPELPSATQWTSVIDQSHGLLFYKTMHDSTIKRVDLKQLDFTVKQERKQKLDS
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHCC
GKFSYQEVGVSN
CCCCHHHCCCCC
>Mature Secondary Structure
MNTNRYKIEIMKKSLLALLLTSAFSYHASACTGITLSTTDNDQVQARTIEWGHSDLNSKL
CCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCCCEEEEEEECCCCCCCCEE
VISPRNHQYTSTMPDQQQGLTWKSQYGFAGISVSDDRFIAEGINEKGLTAGLFYFRNYGS
EECCCCCCCCCCCCCHHHCCEEECCCCEEEEEECCCCHHHCCCCCCCCEEHHHHHHCCCC
LAKYDPKQTANNITDMDFVRWMLTQFETVAEVEAALDKIKIVTVYFDQQGNASPTAHWRV
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCEEEEE
SDKQGNSIVIEIMDHGKINIHKNTAKVLTNSPDYNWQVTNLNNYINLHPGISPPQKINGV
ECCCCCEEEEEEECCCEEEEECCHHEEEECCCCCCEEEEECCCEEEECCCCCCHHHCCCC
EAQSFGVGSNFVGLPGDISPPSRFVRAAFYVNTAPKLNTAEQAVSQAFHILNNFDIPIGS
CCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCC
EFNDKSHIPELPSATQWTSVIDQSHGLLFYKTMHDSTIKRVDLKQLDFTVKQERKQKLDS
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHCC
GKFSYQEVGVSN
CCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]