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The map label for this gene is gdhA [H]

Identifier: 167622530

GI number: 167622530

Start: 708503

End: 709846

Strand: Direct

Name: gdhA [H]

Synonym: Shal_0590

Alternate gene names: 167622530

Gene position: 708503-709846 (Clockwise)

Preceding gene: 167622529

Following gene: 167622531

Centisome position: 13.55

GC content: 48.44

Gene sequence:

>1344_bases
ATGAGCTGCGATCTGACTCTTGATAGTTTTCTTTCTACTGTTGCTGAGCGTAACCCTAACCAGCCTGAGTTTTTGCAAGC
GGTTAAAGAAGTACTCACTTCTATCTGGCCTTTTGTTGCAGCGCATCCTGAATATTTGGATGCAGGGATTTTAGAACGTC
TGGTTGAGCCGGAGCGTCTGATCCAATTCCGTGTTTCTTGGGTTGATGATCAAGGTAAAGTCCAGGTAAACCGTGCTTTT
AGGATCCAGCACAACTCTGCTATTGGTCCTTTCAAAGGCGGCATGCGTTTCCACCCTTCTGTTAACCAGTCAGTACTTAA
GTTCTTGGCTTTCGAGCAAACCTTTAAGAACGCCTTAACGACCTTGCCTATGGGCGGCGGTAAAGGCGGTAGCGATTTCG
ACCCTAAAGGAAAGAGCGATGGCGAAGTTATGCGCTTCTGTCAGGCGTTAATGTTTGAACTGCATCGTCATTTAGGTGCT
GATACCGACGTTCCTGCTGGTGATATCGGTGTGGGCGCCCGCGAAGTGGGCTACATGACCGGTATGATGAAAAAGCTGAC
TAACAGCGCTGAATGTGTGTTTACCGGTAAGGGACTAACATTTGGTGGTAGTTTGATCCGCCCTGAAGCGACTGGCTATG
GTCTACTGTATTTTGTTGAAGCGATGCTAAAAGCAAAAGGTGAAACCTTAGAAGGTAAAACTGTCACTGTTTCTGGCTCG
GGCAACGTGGCGCAATATGCTATCGAAAAAGCGATGGAGATGGGCGGTAAGGTAATTACGGCTTCAGATTCTAGTGGTGT
GGTATTTGATCCAGAAGGCTTTACCCCTGAGAAGCTTGCTAAATTAATGGAAGTTAAAAACGATCGCCGCGGACGTGTTG
CTGATTACGCCTATGAGAATGGGTTAGAGTATGTGACAGGCGGAACGCCATGGCAGTTTGCTGCAGAAGTTGCTCTACCT
TGTGCGACTCAAAACGAGCTAGATAGCGAGCATGCCAAGATGCTAATCGCTAACGGTGTACAGATGGTTGCCGAGGGCGC
GAATATGCCAAGCACGGCTGAAGCTATCGCGTTAATGCATGAAGCTAAAGTGCTGTTTGCACCGGGCAAGGCGGCTAACG
CGGGTGGCGTTGCGACATCAGGTCTAGAGATGAGCCAAAATGCGCTGCGTTTAAGTTGGAGCCGTGATGAAGTGGATATG
CGTTTACGTGATATCATGTACTCTATTCATGCCACCTGTGAGCAGTATGGCCGTGAAGGTACACACATCAACTATGAGTT
GGGTGCTAACGTTGCGGGATTCGTAAAAGTAGCCGACGCCATGAAAGCGCAAGGCGTTTACTAA

Upstream 100 bases:

>100_bases
AATTCCTGATATAAACCTTTCTACACAATAAACTGCGGGTTTACCTAGCGTAATCCAAGCAGTAGCAATCTCAATTCTTT
GTATACCCTGGAGCACCAAA

Downstream 100 bases:

>100_bases
ATCGTTAGTATGTTTTTAAAGGGCCTTAGGGCCCTTTTTTGTTTTCGGCGCTAAACTATCTTTTAGGCGGTGGCTATCAT
CAGCTTAGCTCTGCCTGAAG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSCDLTLDSFLSTVAERNPNQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERLIQFRVSWVDDQGKVQVNRAF
RIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGA
DTDVPAGDIGVGAREVGYMTGMMKKLTNSAECVFTGKGLTFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKTVTVSGS
GNVAQYAIEKAMEMGGKVITASDSSGVVFDPEGFTPEKLAKLMEVKNDRRGRVADYAYENGLEYVTGGTPWQFAAEVALP
CATQNELDSEHAKMLIANGVQMVAEGANMPSTAEAIALMHEAKVLFAPGKAANAGGVATSGLEMSQNALRLSWSRDEVDM
RLRDIMYSIHATCEQYGREGTHINYELGANVAGFVKVADAMKAQGVY

Sequences:

>Translated_447_residues
MSCDLTLDSFLSTVAERNPNQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERLIQFRVSWVDDQGKVQVNRAF
RIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGA
DTDVPAGDIGVGAREVGYMTGMMKKLTNSAECVFTGKGLTFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKTVTVSGS
GNVAQYAIEKAMEMGGKVITASDSSGVVFDPEGFTPEKLAKLMEVKNDRRGRVADYAYENGLEYVTGGTPWQFAAEVALP
CATQNELDSEHAKMLIANGVQMVAEGANMPSTAEAIALMHEAKVLFAPGKAANAGGVATSGLEMSQNALRLSWSRDEVDM
RLRDIMYSIHATCEQYGREGTHINYELGANVAGFVKVADAMKAQGVY
>Mature_446_residues
SCDLTLDSFLSTVAERNPNQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERLIQFRVSWVDDQGKVQVNRAFR
IQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGAD
TDVPAGDIGVGAREVGYMTGMMKKLTNSAECVFTGKGLTFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKTVTVSGSG
NVAQYAIEKAMEMGGKVITASDSSGVVFDPEGFTPEKLAKLMEVKNDRRGRVADYAYENGLEYVTGGTPWQFAAEVALPC
ATQNELDSEHAKMLIANGVQMVAEGANMPSTAEAIALMHEAKVLFAPGKAANAGGVATSGLEMSQNALRLSWSRDEVDMR
LRDIMYSIHATCEQYGREGTHINYELGANVAGFVKVADAMKAQGVY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=363, Percent_Identity=29.2011019283747, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4885281, Length=363, Percent_Identity=28.9256198347107, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788059, Length=446, Percent_Identity=71.0762331838565, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=366, Percent_Identity=29.2349726775956, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=50, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=49.7797356828194, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45549226, Length=383, Percent_Identity=28.9817232375979, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45553475, Length=363, Percent_Identity=29.4765840220386, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24649283, Length=343, Percent_Identity=30.3206997084548, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24649460, Length=318, Percent_Identity=29.2452830188679, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48399; Mature: 48268

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCDLTLDSFLSTVAERNPNQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERL
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
IQFRVSWVDDQGKVQVNRAFRIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALT
HHHHHEEECCCCCEEEEEEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGADTDVPAGDIGVGAREVGYMT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GMMKKLTNSAECVFTGKGLTFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKTVTVSGS
HHHHHHCCCCCEEEECCCCEECCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECC
GNVAQYAIEKAMEMGGKVITASDSSGVVFDPEGFTPEKLAKLMEVKNDRRGRVADYAYEN
CCHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHC
GLEYVTGGTPWQFAAEVALPCATQNELDSEHAKMLIANGVQMVAEGANMPSTAEAIALMH
CCCEEECCCCHHHHHHHHCCCCCCHHCCHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
EAKVLFAPGKAANAGGVATSGLEMSQNALRLSWSRDEVDMRLRDIMYSIHATCEQYGREG
HCEEEECCCCCCCCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
THINYELGANVAGFVKVADAMKAQGVY
CEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SCDLTLDSFLSTVAERNPNQPEFLQAVKEVLTSIWPFVAAHPEYLDAGILERLVEPERL
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
IQFRVSWVDDQGKVQVNRAFRIQHNSAIGPFKGGMRFHPSVNQSVLKFLAFEQTFKNALT
HHHHHEEECCCCCEEEEEEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSDGEVMRFCQALMFELHRHLGADTDVPAGDIGVGAREVGYMT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GMMKKLTNSAECVFTGKGLTFGGSLIRPEATGYGLLYFVEAMLKAKGETLEGKTVTVSGS
HHHHHHCCCCCEEEECCCCEECCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECC
GNVAQYAIEKAMEMGGKVITASDSSGVVFDPEGFTPEKLAKLMEVKNDRRGRVADYAYEN
CCHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHC
GLEYVTGGTPWQFAAEVALPCATQNELDSEHAKMLIANGVQMVAEGANMPSTAEAIALMH
CCCEEECCCCHHHHHHHHCCCCCCHHCCHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
EAKVLFAPGKAANAGGVATSGLEMSQNALRLSWSRDEVDMRLRDIMYSIHATCEQYGREG
HCEEEECCCCCCCCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
THINYELGANVAGFVKVADAMKAQGVY
CEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]