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The map label for this gene is emrB [H]

Identifier: 167622446

GI number: 167622446

Start: 610709

End: 612298

Strand: Direct

Name: emrB [H]

Synonym: Shal_0506

Alternate gene names: 167622446

Gene position: 610709-612298 (Clockwise)

Preceding gene: 167622445

Following gene: 167622447

Centisome position: 11.68

GC content: 49.81

Gene sequence:

>1590_bases
ATGTCTGCTACCCAAGCTGGAGTTGCGAAACTTGATGGTGCGATGACTCAACAGGTCGAGCCGGGTGGCCGCAGAGCGTG
GATCGCGGTATTTGGCGGCCTGATAGGCGCCTTTATGGCGATTTTAGATATTCAGATCACCAATGCGTCGATGAAAGAGA
TCCAAGGCGGCTTAGGTGCCACGCTTGAAGAGGGCTCTTGGATAGCCACCGCTTACTTAGTGGCAGAGATGATCGCCATT
CCGCTATCGGGTTGGCTCAGTAAAGGCTTAGATATACGCCGCTATATGCTGTGGAACAGTGCTATCTTCATCTTTGCATC
GCTGCTGTGCTCTATTGCTTGGAATTTAGAATCGATGATCGCTTTTAGGGCGATGCAGGGGTTCTTTGGCGGCGCCCTGA
TCCCGATGGCGTTTCGGCTCATATTAGAGTATCTACCCGATAGTAAGCGTGCCGTCGGCATGGCGCTGTTTGGGGTTACC
GCGACCTTTGCGCCATCGATTGGCCCCACTCTAGGGGGTTGGTTAACCGAGCAATTTAGCTGGCATTATCTGTTTTATAT
CAATGTGCCACCTGGGATTGTGGTGATGTCGATGCTTGCCTACGGCCTGGTGAAGAAACCCATTAACTGGCCTGAGCTTA
AGAATGTCGATGTTAGCAGTATTATCACCATGGCATTGGGGATGGGTTGTTTAGAGGTGGTGCTAGAGGAGGGAAATCGT
AAGGATTGGTTTGGTTCAGACTTCATACGTAACCTTGCCATCGTCGCGGCGGTGAATATTGCGCTCTTTGTCTATCTGCA
GTTAAAAAGCAAGGCGCCACTGGTTAATTTAAAGCTGCTTGCTAATCGCGATTTTAGCATCTCAACCATTGCCTATTTCT
TACTAGGTTTAGCGCTGTTTTCATCCATCTATATGGTGCCGCTATATCTGTCGCAGATCCAAGATTACAACTCGTTAGAG
ATAGGTGAAGTCTTGATGTGGCTGGGTTTTCCACAGTTGCTGATTTTGCCATTTATGCCGATGTTGATGCAGCGTTTCGA
TAATCGTTATCTAGCGGCATTTGGTTTTTTCATGTTCGGTGTGAGTTATTATATGAACAGCCATATGACTGCCGATTTTG
CTGGGCCTCAGCTAATCGCCTCTATGGTGGTACGTGCTATTGGCCAACCGTTTATCATGGTACCTATTGGCATGTTGGCA
ACGGCTAGGTTGCAAAAGCACGAGAATGCCTCGGCATCGACGGTGTTAAACGTCGTACGAAACTTAGGTGGTGCGGTAGG
GATCGCGATGGTATCAACCCTGACCGATAACTTAGGCCGAGTTCACCTCGCGCGGATGAAAGAAACATTGCCAGCGGTCA
GCCCCTTGGCAAATGATTACTTAAGCAGTACAGCGGCTATGTTGCAAGCGGCGGGGTCAGATCCGGTTACCGCGAGTCAG
CAAGCCTCGGCATTGCTTGGGCAAACCATGCAGCAGCAGGCGATGGTGCAAGCCTATAATGACGTGTTCTTCATCATGGC
AGGTTTACTCGTGGTGGCCGTCGTTGCCGTATTGTCGATACACAAACCGATACCACAGCAAGCTGGTTAG

Upstream 100 bases:

>100_bases
AGCAGGGCAGAATCGTGCCGGGCTTGAGTGCACTCGTGAAAGTCGATACCGCAAGCGATGGCTTAGACTCAGCTATTGTT
GCCAATAGAGTGCAACAGTA

Downstream 100 bases:

>100_bases
ATCTTATTCAAAGAGACTTTCCTCTATCACTCAAAATGAATCCGTTCAACCGCTGCTTTATACGGCAGAGGTTGATTTAA
CGCGACTTTGGCATAGTTAT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSATQAGVAKLDGAMTQQVEPGGRRAWIAVFGGLIGAFMAILDIQITNASMKEIQGGLGATLEEGSWIATAYLVAEMIAI
PLSGWLSKGLDIRRYMLWNSAIFIFASLLCSIAWNLESMIAFRAMQGFFGGALIPMAFRLILEYLPDSKRAVGMALFGVT
ATFAPSIGPTLGGWLTEQFSWHYLFYINVPPGIVVMSMLAYGLVKKPINWPELKNVDVSSIITMALGMGCLEVVLEEGNR
KDWFGSDFIRNLAIVAAVNIALFVYLQLKSKAPLVNLKLLANRDFSISTIAYFLLGLALFSSIYMVPLYLSQIQDYNSLE
IGEVLMWLGFPQLLILPFMPMLMQRFDNRYLAAFGFFMFGVSYYMNSHMTADFAGPQLIASMVVRAIGQPFIMVPIGMLA
TARLQKHENASASTVLNVVRNLGGAVGIAMVSTLTDNLGRVHLARMKETLPAVSPLANDYLSSTAAMLQAAGSDPVTASQ
QASALLGQTMQQQAMVQAYNDVFFIMAGLLVVAVVAVLSIHKPIPQQAG

Sequences:

>Translated_529_residues
MSATQAGVAKLDGAMTQQVEPGGRRAWIAVFGGLIGAFMAILDIQITNASMKEIQGGLGATLEEGSWIATAYLVAEMIAI
PLSGWLSKGLDIRRYMLWNSAIFIFASLLCSIAWNLESMIAFRAMQGFFGGALIPMAFRLILEYLPDSKRAVGMALFGVT
ATFAPSIGPTLGGWLTEQFSWHYLFYINVPPGIVVMSMLAYGLVKKPINWPELKNVDVSSIITMALGMGCLEVVLEEGNR
KDWFGSDFIRNLAIVAAVNIALFVYLQLKSKAPLVNLKLLANRDFSISTIAYFLLGLALFSSIYMVPLYLSQIQDYNSLE
IGEVLMWLGFPQLLILPFMPMLMQRFDNRYLAAFGFFMFGVSYYMNSHMTADFAGPQLIASMVVRAIGQPFIMVPIGMLA
TARLQKHENASASTVLNVVRNLGGAVGIAMVSTLTDNLGRVHLARMKETLPAVSPLANDYLSSTAAMLQAAGSDPVTASQ
QASALLGQTMQQQAMVQAYNDVFFIMAGLLVVAVVAVLSIHKPIPQQAG
>Mature_528_residues
SATQAGVAKLDGAMTQQVEPGGRRAWIAVFGGLIGAFMAILDIQITNASMKEIQGGLGATLEEGSWIATAYLVAEMIAIP
LSGWLSKGLDIRRYMLWNSAIFIFASLLCSIAWNLESMIAFRAMQGFFGGALIPMAFRLILEYLPDSKRAVGMALFGVTA
TFAPSIGPTLGGWLTEQFSWHYLFYINVPPGIVVMSMLAYGLVKKPINWPELKNVDVSSIITMALGMGCLEVVLEEGNRK
DWFGSDFIRNLAIVAAVNIALFVYLQLKSKAPLVNLKLLANRDFSISTIAYFLLGLALFSSIYMVPLYLSQIQDYNSLEI
GEVLMWLGFPQLLILPFMPMLMQRFDNRYLAAFGFFMFGVSYYMNSHMTADFAGPQLIASMVVRAIGQPFIMVPIGMLAT
ARLQKHENASASTVLNVVRNLGGAVGIAMVSTLTDNLGRVHLARMKETLPAVSPLANDYLSSTAAMLQAAGSDPVTASQQ
ASALLGQTMQQQAMVQAYNDVFFIMAGLLVVAVVAVLSIHKPIPQQAG

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=504, Percent_Identity=30.1587301587302, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788710, Length=507, Percent_Identity=29.7830374753452, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=25.1207729468599, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=23.9024390243902, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788392, Length=432, Percent_Identity=23.6111111111111, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=135, Percent_Identity=25.1851851851852, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=26, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57516; Mature: 57385

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATQAGVAKLDGAMTQQVEPGGRRAWIAVFGGLIGAFMAILDIQITNASMKEIQGGLGA
CCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
TLEEGSWIATAYLVAEMIAIPLSGWLSKGLDIRRYMLWNSAIFIFASLLCSIAWNLESMI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRAMQGFFGGALIPMAFRLILEYLPDSKRAVGMALFGVTATFAPSIGPTLGGWLTEQFS
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
WHYLFYINVPPGIVVMSMLAYGLVKKPINWPELKNVDVSSIITMALGMGCLEVVLEEGNR
EEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KDWFGSDFIRNLAIVAAVNIALFVYLQLKSKAPLVNLKLLANRDFSISTIAYFLLGLALF
CCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
SSIYMVPLYLSQIQDYNSLEIGEVLMWLGFPQLLILPFMPMLMQRFDNRYLAAFGFFMFG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VSYYMNSHMTADFAGPQLIASMVVRAIGQPFIMVPIGMLATARLQKHENASASTVLNVVR
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHH
NLGGAVGIAMVSTLTDNLGRVHLARMKETLPAVSPLANDYLSSTAAMLQAAGSDPVTASQ
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QASALLGQTMQQQAMVQAYNDVFFIMAGLLVVAVVAVLSIHKPIPQQAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SATQAGVAKLDGAMTQQVEPGGRRAWIAVFGGLIGAFMAILDIQITNASMKEIQGGLGA
CCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
TLEEGSWIATAYLVAEMIAIPLSGWLSKGLDIRRYMLWNSAIFIFASLLCSIAWNLESMI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRAMQGFFGGALIPMAFRLILEYLPDSKRAVGMALFGVTATFAPSIGPTLGGWLTEQFS
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
WHYLFYINVPPGIVVMSMLAYGLVKKPINWPELKNVDVSSIITMALGMGCLEVVLEEGNR
EEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KDWFGSDFIRNLAIVAAVNIALFVYLQLKSKAPLVNLKLLANRDFSISTIAYFLLGLALF
CCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
SSIYMVPLYLSQIQDYNSLEIGEVLMWLGFPQLLILPFMPMLMQRFDNRYLAAFGFFMFG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VSYYMNSHMTADFAGPQLIASMVVRAIGQPFIMVPIGMLATARLQKHENASASTVLNVVR
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHH
NLGGAVGIAMVSTLTDNLGRVHLARMKETLPAVSPLANDYLSSTAAMLQAAGSDPVTASQ
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QASALLGQTMQQQAMVQAYNDVFFIMAGLLVVAVVAVLSIHKPIPQQAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]