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The map label for this gene is emrA [H]

Identifier: 167622445

GI number: 167622445

Start: 609636

End: 610709

Strand: Direct

Name: emrA [H]

Synonym: Shal_0505

Alternate gene names: 167622445

Gene position: 609636-610709 (Clockwise)

Preceding gene: 167622443

Following gene: 167622446

Centisome position: 11.66

GC content: 48.04

Gene sequence:

>1074_bases
ATGTCTAAAACCAAGTTATTTTTCGGGATTCCTGTTCTTATTATTGCGTTATTAACCGGCGGTTATTATTGGTGGACCCA
AGTACGTAATTTTGAGTCCACAGATAATGCCTATGTAGAATCCGATATCTCTCATATCAGCGTTAAAGTCCCTGGCTATG
TTACTGCCACCTTTGTGACCGATAATCAGCATGTCAAAAAAGGTCAATTACTTGCCCAATTGGAAGATAATCAATTCAGA
GCAAAGGTCGCGCAAAACCAAGCCGTGCTAGCCAGTGCCAAAGCCAAGCTACAGACGCTCAATGCCCAGATTGACCTGCA
GCAAGCGCTTATTACTCAGGCCCAAGCCAGTGTGGTTGCGGCAGAGGCTGAGCGGGTGCGTAGTCAGCAGCAACTGACTC
GAGCCACAAAGCTGAAGGTGAAGAATTACAGTTCTCAAGATGCAGTGGATGAGGCGCAAGCGGCTTTCGAGTCGGCGACA
GCCCATGTCGATGAAATACATGCCGTATTAGTGGCGAAGCAAAGAGAGCTTAAAGTCTTTAATGCACAATTAGTTGAGGC
CGACTCGAGTGTTGAGCAGGCGAAGGCTGGGCTACAGTTAGCGCAAATTCAGCTGGCAGATACCCAAATTACGGCCCCGT
TTACAGGAATAATCGGTAAGCGTGGGGCGCAGCAGGGCCAATATGTACAGCCTGGACAAGCGATTTATAGTCTAGTGCCT
GATGAAGCGGTGTGGATCACCGCTAATTTTAAAGAGACCCAAATTGCCAAGATGCATGCAGGGCAGAAGGTGACGATTGA
GTTAGATGCGTTTTCGGGAAAGGAGTTTTCGGGAGTCATTGATAGCTTATCTCCAGCCTCGGGAGCTAAATTCAGCTTAT
TGCCTGCGGAAAATGCCACGGGTAATTTTACCAAGATCGTACAGCGGATCCCGGTGAGAATTCGCTTAGATTCAAATGCT
GCTGAAAAGCTTGAGCAGGGCAGAATCGTGCCGGGCTTGAGTGCACTCGTGAAAGTCGATACCGCAAGCGATGGCTTAGA
CTCAGCTATTGTTGCCAATAGAGTGCAACAGTAA

Upstream 100 bases:

>100_bases
TATCATTGTTACACCTGTGAAACAGTGGGTGATTTTTTAGCTATCTAATAAATATACTGAGACAGTAGTAAACTATGACT
CACTAATCAAGGAGTCAATC

Downstream 100 bases:

>100_bases
TGTCTGCTACCCAAGCTGGAGTTGCGAAACTTGATGGTGCGATGACTCAACAGGTCGAGCCGGGTGGCCGCAGAGCGTGG
ATCGCGGTATTTGGCGGCCT

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKTKLFFGIPVLIIALLTGGYYWWTQVRNFESTDNAYVESDISHISVKVPGYVTATFVTDNQHVKKGQLLAQLEDNQFR
AKVAQNQAVLASAKAKLQTLNAQIDLQQALITQAQASVVAAEAERVRSQQQLTRATKLKVKNYSSQDAVDEAQAAFESAT
AHVDEIHAVLVAKQRELKVFNAQLVEADSSVEQAKAGLQLAQIQLADTQITAPFTGIIGKRGAQQGQYVQPGQAIYSLVP
DEAVWITANFKETQIAKMHAGQKVTIELDAFSGKEFSGVIDSLSPASGAKFSLLPAENATGNFTKIVQRIPVRIRLDSNA
AEKLEQGRIVPGLSALVKVDTASDGLDSAIVANRVQQ

Sequences:

>Translated_357_residues
MSKTKLFFGIPVLIIALLTGGYYWWTQVRNFESTDNAYVESDISHISVKVPGYVTATFVTDNQHVKKGQLLAQLEDNQFR
AKVAQNQAVLASAKAKLQTLNAQIDLQQALITQAQASVVAAEAERVRSQQQLTRATKLKVKNYSSQDAVDEAQAAFESAT
AHVDEIHAVLVAKQRELKVFNAQLVEADSSVEQAKAGLQLAQIQLADTQITAPFTGIIGKRGAQQGQYVQPGQAIYSLVP
DEAVWITANFKETQIAKMHAGQKVTIELDAFSGKEFSGVIDSLSPASGAKFSLLPAENATGNFTKIVQRIPVRIRLDSNA
AEKLEQGRIVPGLSALVKVDTASDGLDSAIVANRVQQ
>Mature_356_residues
SKTKLFFGIPVLIIALLTGGYYWWTQVRNFESTDNAYVESDISHISVKVPGYVTATFVTDNQHVKKGQLLAQLEDNQFRA
KVAQNQAVLASAKAKLQTLNAQIDLQQALITQAQASVVAAEAERVRSQQQLTRATKLKVKNYSSQDAVDEAQAAFESATA
HVDEIHAVLVAKQRELKVFNAQLVEADSSVEQAKAGLQLAQIQLADTQITAPFTGIIGKRGAQQGQYVQPGQAIYSLVPD
EAVWITANFKETQIAKMHAGQKVTIELDAFSGKEFSGVIDSLSPASGAKFSLLPAENATGNFTKIVQRIPVRIRLDSNAA
EKLEQGRIVPGLSALVKVDTASDGLDSAIVANRVQQ

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=345, Percent_Identity=33.9130434782609, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788711, Length=351, Percent_Identity=31.9088319088319, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=28.4345047923323, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87081951, Length=344, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790024, Length=256, Percent_Identity=25.390625, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790012, Length=248, Percent_Identity=26.6129032258064, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787013, Length=325, Percent_Identity=27.6923076923077, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38660; Mature: 38529

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTKLFFGIPVLIIALLTGGYYWWTQVRNFESTDNAYVESDISHISVKVPGYVTATFVT
CCCCEEEEHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCCCEEEEECCCEEEEEEEE
DNQHVKKGQLLAQLEDNQFRAKVAQNQAVLASAKAKLQTLNAQIDLQQALITQAQASVVA
CCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AEAERVRSQQQLTRATKLKVKNYSSQDAVDEAQAAFESATAHVDEIHAVLVAKQRELKVF
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEH
NAQLVEADSSVEQAKAGLQLAQIQLADTQITAPFTGIIGKRGAQQGQYVQPGQAIYSLVP
HHHEECCCCHHHHHHCCCEEEEEEEECCEECCCHHHHHCCCCCCCCCCCCCCHHHHHHCC
DEAVWITANFKETQIAKMHAGQKVTIELDAFSGKEFSGVIDSLSPASGAKFSLLPAENAT
CCEEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEECCCCC
GNFTKIVQRIPVRIRLDSNAAEKLEQGRIVPGLSALVKVDTASDGLDSAIVANRVQQ
CHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCHHEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SKTKLFFGIPVLIIALLTGGYYWWTQVRNFESTDNAYVESDISHISVKVPGYVTATFVT
CCCEEEEHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCCCEEEEECCCEEEEEEEE
DNQHVKKGQLLAQLEDNQFRAKVAQNQAVLASAKAKLQTLNAQIDLQQALITQAQASVVA
CCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AEAERVRSQQQLTRATKLKVKNYSSQDAVDEAQAAFESATAHVDEIHAVLVAKQRELKVF
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEH
NAQLVEADSSVEQAKAGLQLAQIQLADTQITAPFTGIIGKRGAQQGQYVQPGQAIYSLVP
HHHEECCCCHHHHHHCCCEEEEEEEECCEECCCHHHHHCCCCCCCCCCCCCCHHHHHHCC
DEAVWITANFKETQIAKMHAGQKVTIELDAFSGKEFSGVIDSLSPASGAKFSLLPAENAT
CCEEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEECCCCC
GNFTKIVQRIPVRIRLDSNAAEKLEQGRIVPGLSALVKVDTASDGLDSAIVANRVQQ
CHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCHHEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]