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The map label for this gene is amt [H]

Identifier: 167622355

GI number: 167622355

Start: 486132

End: 487418

Strand: Reverse

Name: amt [H]

Synonym: Shal_0415

Alternate gene names: 167622355

Gene position: 487418-486132 (Counterclockwise)

Preceding gene: 167622356

Following gene: 167622350

Centisome position: 9.33

GC content: 47.4

Gene sequence:

>1287_bases
ATGAGTATTTTAAATACTGGTGATACTGCATTTATGCTTTTGTGCACCGCGCTTGTGCTGTTAATGACTCCGGGGCTTGC
CCTGTTTTATGGGGGATTAGTACGCGAACGTAATATGCTAACTATTATGTTGCAGAATTTTATCTGTATGGGTGTTATCG
CAATTATTTGGGTTTTTGGCGGGTTTAGCCTAGCCTTTGGCGATGATATTGCTGGCGTTATTGGTTCACTCAGCCAATAT
TTTGGAATGTATCATATTGGCGCCGTTCCCAATGAGCATTATGCATCGAGTATTCCTTTCATCATCTTTTTTGCCTACCA
AATGATGTTCGCTATTATCACTCCAGCGCTAATGACTGGTGCATTTGCCGGGCGATTGCAGTTTCCAGCCTATTTAAAAT
TCATCATCCTTTGGTCTCTGCTGGTTTATATTCCTGTGGCACATTGGATGTGGGGCGGAGGCTTTTTAAGCACTATTGGG
GCTGTAGATTTTGCAGGGGGCATTGTAGTACACACCACAGCGGGTATGTCTGCCTTAGCGGCAGCCGTTTACGTTGGTAA
ACGAAGGAGTACCCGCCTTGGCGAAACCGAGTCCGCAGCGAGCTTACCCTTAGTGGCTATTGGCGCAGGGTTACTCTGGT
TTGGTTGGTTTGGGTTCAACTCAGGCGGCGCATACGCTGCCAATCAATTAGCCGCATATGCCTTTACTAACACCACTCTT
GCTGGTTCGACCGCAATGTTAATGTGGATGTTTTGGGAGTGGCGCGAAAACGGCCGCCCCTCTTTTTCAGGCACCTTGGT
AGGCGCGGTAGCGGGGTTAGCAACAATTACTCCTGCAGCGGGATACGTAGAGCCCGCTTCAGCTATTTTTATTGGCCTAA
TCGGCGCTACAGCTTGCTATTTTGCCAAAGCGATTCAAAAAAAACTGGGAGTCGACGATGCGCTTGAAGTTTGGCGAGCT
CATGGTGTCGGTGGCATATTAGGCTCATTACTCGTCGGGCTACTCGCAAGCGATGCTATAGATGAAGTTCAAGCGGGGGC
ATCTCAGTTTTTAATTCAACTTGTTGCCGTTGTTGTTGTCGCGATCTACTCGTGGTTTATCACAACTCTCATCCTAAAAA
TTATCGACAGGAATGGTAGCCTTAAGGTGCCTGATGACATTCAAGAAAGTGGGCTGGACTATGCTGAATTAGGCTCTAAT
GCGTACTCCCGAGATGACGCTAAACCAGTGAGCCAAGCCACACAAACAAAACAGCAGGAGCGTCCCGAAGATAAGCACAC
AAAGTAA

Upstream 100 bases:

>100_bases
CGCAAAAACTTCAGCAGCGAACAATCCAGTAGCTAAACTTAAATCACACTGGCTCTGCAGTATGTAGCCTTAATCTATCC
ATTTTATTTAAGGATGCTTA

Downstream 100 bases:

>100_bases
ATACCTACATATAGCACCACGTTGCTAAACGTGGTGCGACTAAAAACGACTATAGCGCTAAATTGAAGCAGACAAGGTTA
CTCTATCTGCTTGTAACTAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSILNTGDTAFMLLCTALVLLMTPGLALFYGGLVRERNMLTIMLQNFICMGVIAIIWVFGGFSLAFGDDIAGVIGSLSQY
FGMYHIGAVPNEHYASSIPFIIFFAYQMMFAIITPALMTGAFAGRLQFPAYLKFIILWSLLVYIPVAHWMWGGGFLSTIG
AVDFAGGIVVHTTAGMSALAAAVYVGKRRSTRLGETESAASLPLVAIGAGLLWFGWFGFNSGGAYAANQLAAYAFTNTTL
AGSTAMLMWMFWEWRENGRPSFSGTLVGAVAGLATITPAAGYVEPASAIFIGLIGATACYFAKAIQKKLGVDDALEVWRA
HGVGGILGSLLVGLLASDAIDEVQAGASQFLIQLVAVVVVAIYSWFITTLILKIIDRNGSLKVPDDIQESGLDYAELGSN
AYSRDDAKPVSQATQTKQQERPEDKHTK

Sequences:

>Translated_428_residues
MSILNTGDTAFMLLCTALVLLMTPGLALFYGGLVRERNMLTIMLQNFICMGVIAIIWVFGGFSLAFGDDIAGVIGSLSQY
FGMYHIGAVPNEHYASSIPFIIFFAYQMMFAIITPALMTGAFAGRLQFPAYLKFIILWSLLVYIPVAHWMWGGGFLSTIG
AVDFAGGIVVHTTAGMSALAAAVYVGKRRSTRLGETESAASLPLVAIGAGLLWFGWFGFNSGGAYAANQLAAYAFTNTTL
AGSTAMLMWMFWEWRENGRPSFSGTLVGAVAGLATITPAAGYVEPASAIFIGLIGATACYFAKAIQKKLGVDDALEVWRA
HGVGGILGSLLVGLLASDAIDEVQAGASQFLIQLVAVVVVAIYSWFITTLILKIIDRNGSLKVPDDIQESGLDYAELGSN
AYSRDDAKPVSQATQTKQQERPEDKHTK
>Mature_427_residues
SILNTGDTAFMLLCTALVLLMTPGLALFYGGLVRERNMLTIMLQNFICMGVIAIIWVFGGFSLAFGDDIAGVIGSLSQYF
GMYHIGAVPNEHYASSIPFIIFFAYQMMFAIITPALMTGAFAGRLQFPAYLKFIILWSLLVYIPVAHWMWGGGFLSTIGA
VDFAGGIVVHTTAGMSALAAAVYVGKRRSTRLGETESAASLPLVAIGAGLLWFGWFGFNSGGAYAANQLAAYAFTNTTLA
GSTAMLMWMFWEWRENGRPSFSGTLVGAVAGLATITPAAGYVEPASAIFIGLIGATACYFAKAIQKKLGVDDALEVWRAH
GVGGILGSLLVGLLASDAIDEVQAGASQFLIQLVAVVVVAIYSWFITTLILKIIDRNGSLKVPDDIQESGLDYAELGSNA
YSRDDAKPVSQATQTKQQERPEDKHTK

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=412, Percent_Identity=39.3203883495146, Blast_Score=281, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17550182, Length=464, Percent_Identity=25.6465517241379, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=436, Percent_Identity=25.6880733944954, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=25.2336448598131, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564042, Length=443, Percent_Identity=24.3792325056433, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=440, Percent_Identity=33.1818181818182, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=28.0285035629454, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=27.8846153846154, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI161078314, Length=420, Percent_Identity=29.2857142857143, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45780; Mature: 45649

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILNTGDTAFMLLCTALVLLMTPGLALFYGGLVRERNMLTIMLQNFICMGVIAIIWVFG
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GFSLAFGDDIAGVIGSLSQYFGMYHIGAVPNEHYASSIPFIIFFAYQMMFAIITPALMTG
CCHHHHCCHHHHHHHHHHHHHHHEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AFAGRLQFPAYLKFIILWSLLVYIPVAHWMWGGGFLSTIGAVDFAGGIVVHTTAGMSALA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHH
AAVYVGKRRSTRLGETESAASLPLVAIGAGLLWFGWFGFNSGGAYAANQLAAYAFTNTTL
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCC
AGSTAMLMWMFWEWRENGRPSFSGTLVGAVAGLATITPAAGYVEPASAIFIGLIGATACY
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FAKAIQKKLGVDDALEVWRAHGVGGILGSLLVGLLASDAIDEVQAGASQFLIQLVAVVVV
HHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIYSWFITTLILKIIDRNGSLKVPDDIQESGLDYAELGSNAYSRDDAKPVSQATQTKQQE
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHC
RPEDKHTK
CCCCCCCC
>Mature Secondary Structure 
SILNTGDTAFMLLCTALVLLMTPGLALFYGGLVRERNMLTIMLQNFICMGVIAIIWVFG
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GFSLAFGDDIAGVIGSLSQYFGMYHIGAVPNEHYASSIPFIIFFAYQMMFAIITPALMTG
CCHHHHCCHHHHHHHHHHHHHHHEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AFAGRLQFPAYLKFIILWSLLVYIPVAHWMWGGGFLSTIGAVDFAGGIVVHTTAGMSALA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHH
AAVYVGKRRSTRLGETESAASLPLVAIGAGLLWFGWFGFNSGGAYAANQLAAYAFTNTTL
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCC
AGSTAMLMWMFWEWRENGRPSFSGTLVGAVAGLATITPAAGYVEPASAIFIGLIGATACY
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FAKAIQKKLGVDDALEVWRAHGVGGILGSLLVGLLASDAIDEVQAGASQFLIQLVAVVVV
HHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIYSWFITTLILKIIDRNGSLKVPDDIQESGLDYAELGSNAYSRDDAKPVSQATQTKQQE
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHC
RPEDKHTK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]