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The map label for this gene is gmk [H]

Identifier: 167622332

GI number: 167622332

Start: 458897

End: 459556

Strand: Reverse

Name: gmk [H]

Synonym: Shal_0392

Alternate gene names: 167622332

Gene position: 459556-458897 (Counterclockwise)

Preceding gene: 167622337

Following gene: 167622331

Centisome position: 8.79

GC content: 44.09

Gene sequence:

>660_bases
ATGGCTCTAATAGTGCAATCATCGGATATGCAGCTCATGACCGCTCGTGGAAACCTATTTATCGTCTCGGCGCCAAGTGG
CGCAGGTAAATCATCGCTTATTTCAGCCTTGTTACGAGACCAGCCTTTAGATATGCAAGTTTCAGTTTCGCATACCACAA
GACAACCACGTCCTGGCGAAGAGAACGGTCAACACTATCATTTCGTTAATCAAGATGAGTTCAAGGGCTTAATTACCGAT
GGCGCTTTTTTTGAGTGGGCTGAAGTATTTGGAAACTATTATGGTACCTCTCGTGTCACGATTGAGCAGACTCTAGAGCA
AGGTATTGATGTATTTCTCGATATCGACTGGCAAGGTGCTCAACAAGTTAAGCAACTTATGCCTGAATCTATTGGTATTT
TCATTCTACCACCGTCAAAAACTGAGCTAGAACGCCGTCTAACAGGCCGCGGACAAGATAGCGAAGAAGTGATTGCAGGC
CGCATGGCGCAAGCGGTTTCAGAGATGTCGCACTACAATGAATATGACTTCGTTTTAGTAAATGACGACTTCGACAAGGC
ATTGGCCGATCTTTTAGCGATCATTCGCAGTCAAAGATTAACCTCTGCTAGCCAAATTCATACACAAAATGATATGATTA
AAGATCTGTTGGCAGGCTAA

Upstream 100 bases:

>100_bases
TCAATTAGCATACTAATTCACACAACTGCGACTAGCGTAAATATCACCCTAGGCTGTATAATGGCTCGCTTAATCTTTTA
CTCGACCTATTTGAGCTAAC

Downstream 100 bases:

>100_bases
CTGTCTTCATGTACAATTTTGCGTCATTTTTTCAATCGATTAAACACTGGAGTTTCCAAACATGGCTCGCGTAACTGTAG
AAGATGCCGTAAACCAAATC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MALIVQSSDMQLMTARGNLFIVSAPSGAGKSSLISALLRDQPLDMQVSVSHTTRQPRPGEENGQHYHFVNQDEFKGLITD
GAFFEWAEVFGNYYGTSRVTIEQTLEQGIDVFLDIDWQGAQQVKQLMPESIGIFILPPSKTELERRLTGRGQDSEEVIAG
RMAQAVSEMSHYNEYDFVLVNDDFDKALADLLAIIRSQRLTSASQIHTQNDMIKDLLAG

Sequences:

>Translated_219_residues
MALIVQSSDMQLMTARGNLFIVSAPSGAGKSSLISALLRDQPLDMQVSVSHTTRQPRPGEENGQHYHFVNQDEFKGLITD
GAFFEWAEVFGNYYGTSRVTIEQTLEQGIDVFLDIDWQGAQQVKQLMPESIGIFILPPSKTELERRLTGRGQDSEEVIAG
RMAQAVSEMSHYNEYDFVLVNDDFDKALADLLAIIRSQRLTSASQIHTQNDMIKDLLAG
>Mature_218_residues
ALIVQSSDMQLMTARGNLFIVSAPSGAGKSSLISALLRDQPLDMQVSVSHTTRQPRPGEENGQHYHFVNQDEFKGLITDG
AFFEWAEVFGNYYGTSRVTIEQTLEQGIDVFLDIDWQGAQQVKQLMPESIGIFILPPSKTELERRLTGRGQDSEEVIAGR
MAQAVSEMSHYNEYDFVLVNDDFDKALADLLAIIRSQRLTSASQIHTQNDMIKDLLAG

Specific function: Essential for recycling GMP and indirectly, cGMP [H]

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain [H]

Homologues:

Organism=Homo sapiens, GI226874831, Length=174, Percent_Identity=41.9540229885057, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI226874833, Length=174, Percent_Identity=41.9540229885057, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI4504221, Length=174, Percent_Identity=41.9540229885057, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI38570142, Length=182, Percent_Identity=30.2197802197802, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI4505237, Length=176, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI262118259, Length=176, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI262118255, Length=176, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI262118263, Length=176, Percent_Identity=31.8181818181818, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI14780902, Length=197, Percent_Identity=27.9187817258883, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI21361598, Length=195, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI186700629, Length=191, Percent_Identity=22.5130890052356, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI186700627, Length=189, Percent_Identity=22.7513227513227, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI186972120, Length=189, Percent_Identity=22.7513227513227, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21389483, Length=183, Percent_Identity=28.4153005464481, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1790080, Length=205, Percent_Identity=70.2439024390244, Blast_Score=296, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI193203132, Length=180, Percent_Identity=36.1111111111111, Blast_Score=133, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17508835, Length=180, Percent_Identity=36.1111111111111, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557440, Length=181, Percent_Identity=27.0718232044199, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71984092, Length=157, Percent_Identity=31.2101910828025, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988041, Length=196, Percent_Identity=23.469387755102, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71988048, Length=194, Percent_Identity=23.7113402061856, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=43.1693989071038, Blast_Score=155, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21356141, Length=180, Percent_Identity=40, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI116007136, Length=198, Percent_Identity=25.7575757575758, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45554481, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85724806, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45554489, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24640542, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI161077626, Length=180, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665 [H]

Pfam domain/function: PF00625 Guanylate_kin [H]

EC number: =2.7.4.8 [H]

Molecular weight: Translated: 24427; Mature: 24296

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00856 GUANYLATE_KINASE_1 ; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQSSDMQLMTARGNLFIVSAPSGAGKSSLISALLRDQPLDMQVSVSHTTRQPRPGE
CEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
ENGQHYHFVNQDEFKGLITDGAFFEWAEVFGNYYGTSRVTIEQTLEQGIDVFLDIDWQGA
CCCCEEEECCHHHCCEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHCCCEEEEEECCCCH
QQVKQLMPESIGIFILPPSKTELERRLTGRGQDSEEVIAGRMAQAVSEMSHYNEYDFVLV
HHHHHHCHHHCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
NDDFDKALADLLAIIRSQRLTSASQIHTQNDMIKDLLAG
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIVQSSDMQLMTARGNLFIVSAPSGAGKSSLISALLRDQPLDMQVSVSHTTRQPRPGE
EEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
ENGQHYHFVNQDEFKGLITDGAFFEWAEVFGNYYGTSRVTIEQTLEQGIDVFLDIDWQGA
CCCCEEEECCHHHCCEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHCCCEEEEEECCCCH
QQVKQLMPESIGIFILPPSKTELERRLTGRGQDSEEVIAGRMAQAVSEMSHYNEYDFVLV
HHHHHHCHHHCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
NDDFDKALADLLAIIRSQRLTSASQIHTQNDMIKDLLAG
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]