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The map label for this gene is spoT [H]

Identifier: 167622330

GI number: 167622330

Start: 456435

End: 458540

Strand: Reverse

Name: spoT [H]

Synonym: Shal_0390

Alternate gene names: 167622330

Gene position: 458540-456435 (Counterclockwise)

Preceding gene: 167622331

Following gene: 167622329

Centisome position: 8.77

GC content: 47.67

Gene sequence:

>2106_bases
TTGTATCTGTTTGAAGGTCTCAAGGAGTCTGCATCAGGTTATTTAGAACCTGAACAGGTAGAATTACTCAAGCAGGCCTA
TCAGGTGGCGCGCAATGCCCATGAAGGTCAGATGCGCACGAGTGGCGAACCTTATATTACCCATCCGGTTGCGGTCGCCC
GCATCCTGGCCGATATGCGTCTCGATCATGAGACGCTTATGGCCGCACTCTTGCACGACACTATCGAAGACACCCCGGTA
ACTAAAGAAGAACTGGCTGAGCTATTTAGCGAGTCAATTGCTGAACTGGTTGAAGGCGTCTCCAAGCTCGATAAACTCAA
ATTTCGCGATAAGAAAGAAGCCCAAGCTGAAAACTTCCGCAAAATGATGATGGCGATGACCCAAGATATTCGAGTCATCT
TGATTAAGCTTGCCGATAGAACCCACAATATGCGCACCTTAGGTGCTCTACGGCCCGATAAGCGCCGTCGCATCGCCCGA
GAAACCCTCGAAATTTACGCTCCAATCGCCAACCGTCTTGGTATTCATAATATTAAGATTGAGTTAGAAGATTTAGGCTT
CCAAGCCTATTATCCAATGCGATATCGCGTACTCAAAGAGGTGGTGAAAGCCGCTCGTGGTAATCGTAAAGAACTTATTC
AAAGTATCGAAGCTGCAATCCATACTCGACTCGAAGACACTGGCCTTGAAGGCGCTGTAAAAGGTCGTGAAAAACATCTT
TATTCCATCTATAACAAGATGCGCAATAAAGAGCTGCAGTTCCAAGAAGTCATGGATATCTATGCCTTTAGGGTCATCGT
TGACTCTATCGATACTTGCTATCGCGTTATGGGTGCCATGCATGGCCTTTATAAGCCTCACCCTGGTCGCTTCAAAGATT
ATATCGCTATCCCTAAAGCCAACGGTTATCAGTCACTGCACACTTCACTTTTTGGCCCTCACGGCGTGCCGGTTGAGATC
CAAATTCGTACCGAAGATATGGACCAGATGGCAGATAAAGGGGTCGCGGCTCATTGGCTATACAAGAAAGGCAGCTCGGC
AGAGCAAGGCACCACCACTCAAGTGCGTGCCCGTAAATGGATGCAAAGCTTGCTTGAACTGCAGCAAAGCGCCAGCACCT
CTTTTGAATTTGTTGAAAACTTTAAGACTGAGCTTTTCCCTGAAGAGATCTATGTCTTTACCCCTGAAGGCCGTATTTTA
GAGCTTCCTGTGGGAGCAACCCCGGTCGATTTTGCTTACGAAGTTCACACCGATGTAGGTAATACCTGTGTTGGCGCCAA
AGTGAACCGTCAAGCCTACCCATTAAGTCAGCCATTGATCTCAGGCCAAACCGTCGAGATCATTATCGCTAACGGCGCAC
GCCCGAATGCAGCTTGGCTTAACTTTGTGGTGACGGGTAAAGCACGAGCTAAAATCCGTCAGCTACTGAAGAGTTTAAAA
GAGGACGACGCTATTTTGCTTGGTAAGCGTCTGCTCAATCATGCGCTGGGAGAAGCTAAGCTTGATAGCTTGTCGGCAGA
ACAGATTGATAAAGTCGTTAAAGATACCAAGCATGATTCACTCGACTCTTTGCTTGCCGACATTGGCTTAGGCAATGCTA
TGAGCATCGTTATTGCTCAGCGACTTCTTGGCGATCAAATTGCTCCTGAAGAGTCAAAAGAAAACCATACCATGTCAATA
CGCGGCGCCGAAGGTATGCTAGTGACCTTCGCAAATTGCTGTCGCCCAATTCCGGGTGATGCGGTTATCGCCCACGTCAG
CCCTGGCAAAGGCCTAGTGGTACACATGGAAAGCTGCGCTAACATTCGTGGCTACCAAGGTGAGCCTGACAAGTACATTC
CAGTACAGTGGGATAGTGCCGAAGGCGTTGAGTATCAAGCTAACTTGCGCGTAGAAATAGTTAACCACCAAGGTGCACTC
GCAAAAATCACCTCGATTATTGCTTCTGCTGGCTCAAATATTCATAACTTAACCACAGAAGAGCGTGATGGGCGTGTTTT
CCTCATCAACTTGCGGATCTCTGTGCGTGACCGTATTCATTTAGCTAACGTGATGCGTCGAATTAGGGTACTTCCTGAAG
TGTTACGTACATCTCGAAACCGTTAA

Upstream 100 bases:

>100_bases
GCCGATGAGCGCCAAACAGTTCGTGAACGTGAAGCAGCTGAAATCGCTGCTGTTGCTGCCATCGCTGAAGGCCGTAACGC
AATATAAGGAGTAGAGTCAC

Downstream 100 bases:

>100_bases
TTATTTCAATACGCAGATAGAGGAAAGATCATGGCAGAAAAGATCATCATCGCAACCGATAAAGCGCCACAAGCAATTGG
CACATACTCTCAAGCAGTAA

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQVARNAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTPV
TKEELAELFSESIAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKIELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQSIEAAIHTRLEDTGLEGAVKGREKHL
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKWMQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRIL
ELPVGATPVDFAYEVHTDVGNTCVGAKVNRQAYPLSQPLISGQTVEIIIANGARPNAAWLNFVVTGKARAKIRQLLKSLK
EDDAILLGKRLLNHALGEAKLDSLSAEQIDKVVKDTKHDSLDSLLADIGLGNAMSIVIAQRLLGDQIAPEESKENHTMSI
RGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCANIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGAL
AKITSIIASAGSNIHNLTTEERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR

Sequences:

>Translated_701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQVARNAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTPV
TKEELAELFSESIAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKIELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQSIEAAIHTRLEDTGLEGAVKGREKHL
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKWMQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRIL
ELPVGATPVDFAYEVHTDVGNTCVGAKVNRQAYPLSQPLISGQTVEIIIANGARPNAAWLNFVVTGKARAKIRQLLKSLK
EDDAILLGKRLLNHALGEAKLDSLSAEQIDKVVKDTKHDSLDSLLADIGLGNAMSIVIAQRLLGDQIAPEESKENHTMSI
RGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCANIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGAL
AKITSIIASAGSNIHNLTTEERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
>Mature_701_residues
MYLFEGLKESASGYLEPEQVELLKQAYQVARNAHEGQMRTSGEPYITHPVAVARILADMRLDHETLMAALLHDTIEDTPV
TKEELAELFSESIAELVEGVSKLDKLKFRDKKEAQAENFRKMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIAR
ETLEIYAPIANRLGIHNIKIELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQSIEAAIHTRLEDTGLEGAVKGREKHL
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKANGYQSLHTSLFGPHGVPVEI
QIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKWMQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRIL
ELPVGATPVDFAYEVHTDVGNTCVGAKVNRQAYPLSQPLISGQTVEIIIANGARPNAAWLNFVVTGKARAKIRQLLKSLK
EDDAILLGKRLLNHALGEAKLDSLSAEQIDKVVKDTKHDSLDSLLADIGLGNAMSIVIAQRLLGDQIAPEESKENHTMSI
RGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCANIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGAL
AKITSIIASAGSNIHNLTTEERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=703, Percent_Identity=68.7055476529161, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=700, Percent_Identity=31.2857142857143, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24650996, Length=164, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 78820; Mature: 78820

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFEGLKESASGYLEPEQVELLKQAYQVARNAHEGQMRTSGEPYITHPVAVARILADMR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTPVTKEELAELFSESIAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQSIEAAIHTRLEDTGLEGAVKGREKHL
EEEECCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHCHHHHHH
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
MQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRILELPVGATPVDFAYEVHTDVG
HHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCEEEEECCCC
NTCVGAKVNRQAYPLSQPLISGQTVEIIIANGARPNAAWLNFVVTGKARAKIRQLLKSLK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHC
EDDAILLGKRLLNHALGEAKLDSLSAEQIDKVVKDTKHDSLDSLLADIGLGNAMSIVIAQ
CCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
RLLGDQIAPEESKENHTMSIRGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCA
HHHCCCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCCCCCEEEEEECCCCCEEEEEHHHC
NIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGALAKITSIIASAGSNIHNLTTE
CCCCCCCCCCCEEEEEECCCCCCEEEECEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFEGLKESASGYLEPEQVELLKQAYQVARNAHEGQMRTSGEPYITHPVAVARILADMR
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHHC
LDHETLMAALLHDTIEDTPVTKEELAELFSESIAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQSIEAAIHTRLEDTGLEGAVKGREKHL
EEEECCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHCHHHHHH
YSIYNKMRNKELQFQEVMDIYAFRVIVDSIDTCYRVMGAMHGLYKPHPGRFKDYIAIPKA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NGYQSLHTSLFGPHGVPVEIQIRTEDMDQMADKGVAAHWLYKKGSSAEQGTTTQVRARKW
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
MQSLLELQQSASTSFEFVENFKTELFPEEIYVFTPEGRILELPVGATPVDFAYEVHTDVG
HHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCEEEEECCCC
NTCVGAKVNRQAYPLSQPLISGQTVEIIIANGARPNAAWLNFVVTGKARAKIRQLLKSLK
CCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHC
EDDAILLGKRLLNHALGEAKLDSLSAEQIDKVVKDTKHDSLDSLLADIGLGNAMSIVIAQ
CCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
RLLGDQIAPEESKENHTMSIRGAEGMLVTFANCCRPIPGDAVIAHVSPGKGLVVHMESCA
HHHCCCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCCCCCEEEEEECCCCCEEEEEHHHC
NIRGYQGEPDKYIPVQWDSAEGVEYQANLRVEIVNHQGALAKITSIIASAGSNIHNLTTE
CCCCCCCCCCCEEEEEECCCCCCEEEECEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCC
ERDGRVFLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]