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The map label for this gene is glnA [H]

Identifier: 167622294

GI number: 167622294

Start: 416340

End: 417749

Strand: Reverse

Name: glnA [H]

Synonym: Shal_0353

Alternate gene names: 167622294

Gene position: 417749-416340 (Counterclockwise)

Preceding gene: 167622298

Following gene: 167622293

Centisome position: 7.99

GC content: 45.25

Gene sequence:

>1410_bases
ATGTCAGCAGAATCAGTTTTACAACAACTAAAAGAGTTAGAAGTTAAGTTTGTTGATTTACGTTTTACCGATACGATAGG
TAAAGAGCAGCACGTTACAATCCCAACTCACCAAGTTGATGCTGACTTTTTTGAAGACGGTAAAATGTTTGACGGTTCTT
CAATCTCAGGTTGGAAAGGCATTAACGAATCAGACATGGTATTGATGCCTGATCCAGCAAGCTTTGTTCTTGACCCTTTT
ACCGCTGAAACGACTGCAAACATTCGTTGTGACATCCTAAACCCTGGCACAATGACAGGTTATAACCGTGACCCACGTTC
AATTGCAAAGAAAGCTGAAGATTACCTACGTTCAACAGGTATTGCCGATACAGTATTGGTTGGTCCAGAGCCAGAATTCT
TCCTATTTGACGATGTCAAGTTCGGCGCTGACATGTCGGGTTGTTTCTACAAGGTTGACGCTGAAGAAGCGGCTTGGAAC
TCAGGTACCAGCTATGAAAATGGCAACAAGGGTCACCGTCCAGGCGTTAAAGGCGGTTACTTCCCAGTAGCACCAGTAGA
TTCATCACAAGATATCCGTAGTGCTATGTGTTTAGTACTAGAAGAGATGGGCCAAGTTGTTGAAGCACACCACCATGAAG
TTGCTACAGCGGGCCAGAACGAAATCGCAACACGTTTCAATACACTAACACTGAAGGCCGACGAAGTTCAGGTACTTAAG
TATGTTGTACATAACGTTGCTCACGCTTACAACAAGACTGCGACATTCATGCCTAAACCAATCGTTGGTGACAACGGTAG
CGGTATGCACGTGCATCAGTCTCTTGCAAAAGACGGCGTGAACTTATTCGCTGGTGACAAGTATGGCGGATTGAGCGAAA
CAGCACTATTTTACATCGGTGGTGTTATTAAGCATGCTCGCGCAATCAACGCATTTGCTAACCCATCGACAAACTCATAT
AAGCGCCTTGTACCACACTTCGAAGCGCCAGTTATGCTTGCTTACTCAGCAGCTAACCGCTCTGCTTCTATCCGTATCCC
AGTGGTACCAAGTGCAAAAGGTCGTCGTATCGAAATCCGTTTTGGTGACCCAGTAGCAAACCCATACTTAGCATTCTCTG
CAATGCTAATGGCGGGCCTTGACGGCATCCAAAACAAGATCCACCCAGGTGAGGCAATGGACAAAGATCTATATGATCTT
CCAGCAGAAGAAGCGGCTGAAATCCCAACAGTTGCCACTTCACTAGAAAATGCACTTGAGTGTCTAGACGCTGACCGTGA
GTTCTTGACTCGTGGCGACGTATTCACTAACGACTTTATCGATTCTTACATCACACTTAAGTCTGCAGACGTTGAGCGTA
TCAACCAAACTACTCACCCAGTTGAATTCGAGCTTTACTACAGCTTGTAA

Upstream 100 bases:

>100_bases
TGGCACGAGTCTCGCTATATGAAGACCATAATCGCATTAAGCGCTAAATATTAAAGATAAAACAAAGGGACGACCCTTTA
CCCTACTCGGAGACTTTAGA

Downstream 100 bases:

>100_bases
TTATCTGAGATGACTCGCTAACCGCTAGAGTGGTTAGCCTTACAGCCCTAAACAGCAATGTTTGGGGCTTTTTTTTTACC
TTGTTCGCCTGTCTATCTAC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAESVLQQLKELEVKFVDLRFTDTIGKEQHVTIPTHQVDADFFEDGKMFDGSSISGWKGINESDMVLMPDPASFVLDPF
TAETTANIRCDILNPGTMTGYNRDPRSIAKKAEDYLRSTGIADTVLVGPEPEFFLFDDVKFGADMSGCFYKVDAEEAAWN
SGTSYENGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
YVVHNVAHAYNKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALFYIGGVIKHARAINAFANPSTNSY
KRLVPHFEAPVMLAYSAANRSASIRIPVVPSAKGRRIEIRFGDPVANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDL
PAEEAAEIPTVATSLENALECLDADREFLTRGDVFTNDFIDSYITLKSADVERINQTTHPVEFELYYSL

Sequences:

>Translated_469_residues
MSAESVLQQLKELEVKFVDLRFTDTIGKEQHVTIPTHQVDADFFEDGKMFDGSSISGWKGINESDMVLMPDPASFVLDPF
TAETTANIRCDILNPGTMTGYNRDPRSIAKKAEDYLRSTGIADTVLVGPEPEFFLFDDVKFGADMSGCFYKVDAEEAAWN
SGTSYENGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
YVVHNVAHAYNKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALFYIGGVIKHARAINAFANPSTNSY
KRLVPHFEAPVMLAYSAANRSASIRIPVVPSAKGRRIEIRFGDPVANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDL
PAEEAAEIPTVATSLENALECLDADREFLTRGDVFTNDFIDSYITLKSADVERINQTTHPVEFELYYSL
>Mature_468_residues
SAESVLQQLKELEVKFVDLRFTDTIGKEQHVTIPTHQVDADFFEDGKMFDGSSISGWKGINESDMVLMPDPASFVLDPFT
AETTANIRCDILNPGTMTGYNRDPRSIAKKAEDYLRSTGIADTVLVGPEPEFFLFDDVKFGADMSGCFYKVDAEEAAWNS
GTSYENGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLKY
VVHNVAHAYNKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALFYIGGVIKHARAINAFANPSTNSYK
RLVPHFEAPVMLAYSAANRSASIRIPVVPSAKGRRIEIRFGDPVANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDLP
AEEAAEIPTVATSLENALECLDADREFLTRGDVFTNDFIDSYITLKSADVERINQTTHPVEFELYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=76.1194029850746, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=174, Percent_Identity=31.6091954022989, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51459; Mature: 51328

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAESVLQQLKELEVKFVDLRFTDTIGKEQHVTIPTHQVDADFFEDGKMFDGSSISGWKG
CCHHHHHHHHHHCCEEEEEEEEECCCCCCCEEECCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDPASFVLDPFTAETTANIRCDILNPGTMTGYNRDPRSIAKKAEDYLRSTG
CCCCCEEEECCCHHHEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLVGPEPEFFLFDDVKFGADMSGCFYKVDAEEAAWNSGTSYENGNKGHRPGVKGGY
CCCEEEECCCCCEEEEECCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEEECHHHHHHH
YVVHNVAHAYNKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALFYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
GVIKHARAINAFANPSTNSYKRLVPHFEAPVMLAYSAANRSASIRIPVVPSAKGRRIEIR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
FGDPVANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDLPAEEAAEIPTVATSLENALE
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHCCCHHHHHHHHHHH
CLDADREFLTRGDVFTNDFIDSYITLKSADVERINQTTHPVEFELYYSL
HHCCCHHHHHCCCCHHHHHHHHEEEEECCCHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure 
SAESVLQQLKELEVKFVDLRFTDTIGKEQHVTIPTHQVDADFFEDGKMFDGSSISGWKG
CHHHHHHHHHHCCEEEEEEEEECCCCCCCEEECCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVLMPDPASFVLDPFTAETTANIRCDILNPGTMTGYNRDPRSIAKKAEDYLRSTG
CCCCCEEEECCCHHHEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLVGPEPEFFLFDDVKFGADMSGCFYKVDAEEAAWNSGTSYENGNKGHRPGVKGGY
CCCEEEECCCCCEEEEECCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEEECHHHHHHH
YVVHNVAHAYNKTATFMPKPIVGDNGSGMHVHQSLAKDGVNLFAGDKYGGLSETALFYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
GVIKHARAINAFANPSTNSYKRLVPHFEAPVMLAYSAANRSASIRIPVVPSAKGRRIEIR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEE
FGDPVANPYLAFSAMLMAGLDGIQNKIHPGEAMDKDLYDLPAEEAAEIPTVATSLENALE
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHCCCHHHHHHHHHHH
CLDADREFLTRGDVFTNDFIDSYITLKSADVERINQTTHPVEFELYYSL
HHCCCHHHHHCCCCHHHHHHHHEEEEECCCHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]