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The map label for this gene is glnL [H]

Identifier: 167622283

GI number: 167622283

Start: 406575

End: 407615

Strand: Reverse

Name: glnL [H]

Synonym: Shal_0342

Alternate gene names: 167622283

Gene position: 407615-406575 (Counterclockwise)

Preceding gene: 167622284

Following gene: 167622282

Centisome position: 7.8

GC content: 48.51

Gene sequence:

>1041_bases
ATGGATACAAGTTTATTGCTCAATCATTTAGTAACGGCTGTGCTCGTTATCAATGAAGAGCTTGAGTTACTCTACGTTAA
TGCGGCCGCAGAGCAATTGCTCGGTGTTGGCAGTAACCGCCTGAGCGAACACCCCTTACCCGAGCACTATCAATTACTCG
GCGTTGACCCTCAGGTACTAAAACTGGCTATTTTCGAAGGCCAAGGCTTGAGTATTAATACCGTGCCCTTAGTCACTCTC
GATGCTCAACACCATACTGTCGACGTCACGCTGACGCCTCTTGAGCAAGAGAAGTACCTTGCTCTGATTGAGTTACGTCA
GGTCGATCAGCAGCGCCGGATCCACCAACAGTTAACCCAAGATGCTCAGCAACAGGCGGCGCAATATTTAGTGCGCAACC
TCGCCCATGAGATCAAGAATCCATTAGGTGGATTACGCGGCGCAGCTCAGCTGTTGTCCCGAGAGCTAAAAGAACCTGAA
CTTCACGAGTTTACCGATCTTATTATTGAGCAGGCCGATAGGTTGCGTAACTTAGTCGATCGCTTACTCGGCCCGCAGAG
GCCGACTCAACATAGCCTGTACAATATCCACGAAGTGATCCAAAAAGTTGTCCAGCTAGTGAATGTTACCCTGCCAAGCA
ATATTGAACTCACTCAAGATTATGACCCCTCGATCCCTGATATTGAGATGGATCCAGACCAGCTGCAGCAAGCTATTCTC
AATATTGTACAAAACGCGATACAAGCCTTAGAGCCTAAGGGTGGCAATATTCGCCTTAAGACCCGTACCCAACATCAAAT
AACCATAGGTACCAAGCGCCACAAGTTAGTGCTTATGCTATCTATCATCGATGATGGACCGGGCATTCAGCCCGAACTAA
TGGACACCCTATTTTATCCCATGGTGACAGGACGAGAACAAGGCTCAGGCCTTGGACTATCAATCGCCCATAACTTTGCC
CGCTTGCATGGCGGCCGCATCGATTGCGACTCAACCGTCGGTCACACTGAATTTACCATCACCTTACCCATCAATAGTTA
A

Upstream 100 bases:

>100_bases
CGATGAAGTCTAGTTGCAGGCTGCACCACTTTGGTGCACCATAGTGGTGCGATCAACTGAAATGCCCAAACTGGTGCATC
TGCATAATTCGGAGTAGCTG

Downstream 100 bases:

>100_bases
TAAGAGGAAGACCTAGAATGACTGAACAAGTATGGATCCTCGACGACGATAGCTCGATACGCTGGGTATTAGAAAAAGCG
CTGCAAAGTGCTAAATTTAG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDTSLLLNHLVTAVLVINEELELLYVNAAAEQLLGVGSNRLSEHPLPEHYQLLGVDPQVLKLAIFEGQGLSINTVPLVTL
DAQHHTVDVTLTPLEQEKYLALIELRQVDQQRRIHQQLTQDAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKEPE
LHEFTDLIIEQADRLRNLVDRLLGPQRPTQHSLYNIHEVIQKVVQLVNVTLPSNIELTQDYDPSIPDIEMDPDQLQQAIL
NIVQNAIQALEPKGGNIRLKTRTQHQITIGTKRHKLVLMLSIIDDGPGIQPELMDTLFYPMVTGREQGSGLGLSIAHNFA
RLHGGRIDCDSTVGHTEFTITLPINS

Sequences:

>Translated_346_residues
MDTSLLLNHLVTAVLVINEELELLYVNAAAEQLLGVGSNRLSEHPLPEHYQLLGVDPQVLKLAIFEGQGLSINTVPLVTL
DAQHHTVDVTLTPLEQEKYLALIELRQVDQQRRIHQQLTQDAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKEPE
LHEFTDLIIEQADRLRNLVDRLLGPQRPTQHSLYNIHEVIQKVVQLVNVTLPSNIELTQDYDPSIPDIEMDPDQLQQAIL
NIVQNAIQALEPKGGNIRLKTRTQHQITIGTKRHKLVLMLSIIDDGPGIQPELMDTLFYPMVTGREQGSGLGLSIAHNFA
RLHGGRIDCDSTVGHTEFTITLPINS
>Mature_346_residues
MDTSLLLNHLVTAVLVINEELELLYVNAAAEQLLGVGSNRLSEHPLPEHYQLLGVDPQVLKLAIFEGQGLSINTVPLVTL
DAQHHTVDVTLTPLEQEKYLALIELRQVDQQRRIHQQLTQDAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKEPE
LHEFTDLIIEQADRLRNLVDRLLGPQRPTQHSLYNIHEVIQKVVQLVNVTLPSNIELTQDYDPSIPDIEMDPDQLQQAIL
NIVQNAIQALEPKGGNIRLKTRTQHQITIGTKRHKLVLMLSIIDDGPGIQPELMDTLFYPMVTGREQGSGLGLSIAHNFA
RLHGGRIDCDSTVGHTEFTITLPINS

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=343, Percent_Identity=50.7288629737609, Blast_Score=333, Evalue=9e-93,
Organism=Escherichia coli, GI1790436, Length=240, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788549, Length=351, Percent_Identity=23.9316239316239, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786600, Length=357, Percent_Identity=26.3305322128852, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=216, Percent_Identity=31.4814814814815, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=28.6324786324786, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38703; Mature: 38703

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSLLLNHLVTAVLVINEELELLYVNAAAEQLLGVGSNRLSEHPLPEHYQLLGVDPQVL
CCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCHHEEECCCHHHE
KLAIFEGQGLSINTVPLVTLDAQHHTVDVTLTPLEQEKYLALIELRQVDQQRRIHQQLTQ
EEEEECCCCCEEECEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKEPELHEFTDLIIEQADRLRNLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLLGPQRPTQHSLYNIHEVIQKVVQLVNVTLPSNIELTQDYDPSIPDIEMDPDQLQQAIL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
NIVQNAIQALEPKGGNIRLKTRTQHQITIGTKRHKLVLMLSIIDDGPGIQPELMDTLFYP
HHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNFARLHGGRIDCDSTVGHTEFTITLPINS
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MDTSLLLNHLVTAVLVINEELELLYVNAAAEQLLGVGSNRLSEHPLPEHYQLLGVDPQVL
CCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCHHEEECCCHHHE
KLAIFEGQGLSINTVPLVTLDAQHHTVDVTLTPLEQEKYLALIELRQVDQQRRIHQQLTQ
EEEEECCCCCEEECEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKEPELHEFTDLIIEQADRLRNLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLLGPQRPTQHSLYNIHEVIQKVVQLVNVTLPSNIELTQDYDPSIPDIEMDPDQLQQAIL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
NIVQNAIQALEPKGGNIRLKTRTQHQITIGTKRHKLVLMLSIIDDGPGIQPELMDTLFYP
HHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNFARLHGGRIDCDSTVGHTEFTITLPINS
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]