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The map label for this gene is glnG [H]

Identifier: 167622282

GI number: 167622282

Start: 405148

End: 406557

Strand: Reverse

Name: glnG [H]

Synonym: Shal_0341

Alternate gene names: 167622282

Gene position: 406557-405148 (Counterclockwise)

Preceding gene: 167622283

Following gene: 167622279

Centisome position: 7.78

GC content: 52.06

Gene sequence:

>1410_bases
ATGACTGAACAAGTATGGATCCTCGACGACGATAGCTCGATACGCTGGGTATTAGAAAAAGCGCTGCAAAGTGCTAAATT
TAGCTGCGCTAGCTTTGCCGCTGCCGAGTCTCTTTGGCAAGCCTTGGAGATGACGCAGCCTCAAGTCATTGTCTCTGACA
TACGTATGCCCGGTACCGACGGTTTGACTCTCTTAGAGCGTCTGCAAAATCATTATCCGCATATTCCGGTTATTATCATG
ACGGCACATTCCGATCTCGACAGCGCTGTGAGCGCCTATCAGGCCGGAGCCTTTGAATATTTACCCAAGCCTTTCGATAT
CGACGAAGCCATCTCGCTAGTTGACCGTGCTCTCACCCATGCAAAAGAGCAGAGCTCGAGTATCACCGTTGAAGAGCCGC
TAGTCGCCACACCAGAGATCATAGGTGAAGCCCCTGCGATGCAGGAGGTATTTAGAGCCATTGGTCGCCTTTCCCGCTCC
TCGATTAGCGTATTAATCAATGGTCAATCGGGTACGGGTAAGGAACTCGTCGCTAGCGCGCTGCATAAACATAGTCCACG
CAAGGGTAAGCCATTTATTGCGATAAACATGGCGGCGATCCCTAAAGATCTGATTGAGTCAGAGCTATTTGGCCATGAGA
AAGGCGCCTTTACCGGTGCTGGCAGCGTCCGCCAGGGGCGCTTCGAGCAAGCCAACGGCGGCACCCTATTCTTGGATGAA
ATTGGCGATATGCCTCTGGACGTTCAGACTCGATTACTGCGAGTACTAGCCGATGGTCAATTTTATCGCGTGGGTGGCCA
CTCACCAGTACAAGTGGATGTGCGCATTATCGCCGCAACCCATCAAGACCTTGAACAACTCGTGTATCAGGGGGGATTTC
GTGAAGATCTATTCCATCGCTTAAACGTCATTCGCGTGCATCTGCCGCCGCTGTCTCAGCGTCGAGAGGATATTCCTCAG
CTCGCACGTCACTTCCTGATGATCGCCGCCAAAGAGATCGCCGTAGAGCCTAAAGTGCTGACTAAAGAGGCCGCGACTAA
GCTATCGCAACTACCGTGGCCAGGTAACGTTCGTCAGCTTGAAAACACCTGCCGCTGGCTAACGGTAATGGCCTCGGGTC
AGGAGATCTTGCCACTCGACCTACCACCAGAGCTATTACAAGAGCCTAAACTCAATAACGCACAAACGAGTGACAGTGAT
GACTGGCAAGGCGCGTTGAAGCTATTCATCGATCAGCGCCTCAGCGATGGCGATAGCGATCTGCTCACCGAAGTGCAGCC
AGCTTTCGAGCGCATCTTACTGCAAACCGCCCTTAAGCATACCAATGGTCATAAGCAAGAAGCGGCAAAACGCCTAGGTT
GGGGGCGCAACACCTTAACCCGAAAATTGAAAGAGCTAGAGATGGATTAG

Upstream 100 bases:

>100_bases
CCCGCTTGCATGGCGGCCGCATCGATTGCGACTCAACCGTCGGTCACACTGAATTTACCATCACCTTACCCATCAATAGT
TAATAAGAGGAAGACCTAGA

Downstream 100 bases:

>100_bases
CAGCTCCCTCAATACGGCTTGCAGTAAAGAATCCACCTTCTGAAGTAGATGCTTTAGGGCTGAAAACAAAAAAGGAGATC
AACAGGATCTCCTTTTTTGA

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTEQVWILDDDSSIRWVLEKALQSAKFSCASFAAAESLWQALEMTQPQVIVSDIRMPGTDGLTLLERLQNHYPHIPVIIM
TAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTHAKEQSSSITVEEPLVATPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVASALHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLEQLVYQGGFREDLFHRLNVIRVHLPPLSQRREDIPQ
LARHFLMIAAKEIAVEPKVLTKEAATKLSQLPWPGNVRQLENTCRWLTVMASGQEILPLDLPPELLQEPKLNNAQTSDSD
DWQGALKLFIDQRLSDGDSDLLTEVQPAFERILLQTALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD

Sequences:

>Translated_469_residues
MTEQVWILDDDSSIRWVLEKALQSAKFSCASFAAAESLWQALEMTQPQVIVSDIRMPGTDGLTLLERLQNHYPHIPVIIM
TAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTHAKEQSSSITVEEPLVATPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVASALHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLEQLVYQGGFREDLFHRLNVIRVHLPPLSQRREDIPQ
LARHFLMIAAKEIAVEPKVLTKEAATKLSQLPWPGNVRQLENTCRWLTVMASGQEILPLDLPPELLQEPKLNNAQTSDSD
DWQGALKLFIDQRLSDGDSDLLTEVQPAFERILLQTALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD
>Mature_468_residues
TEQVWILDDDSSIRWVLEKALQSAKFSCASFAAAESLWQALEMTQPQVIVSDIRMPGTDGLTLLERLQNHYPHIPVIIMT
AHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTHAKEQSSSITVEEPLVATPEIIGEAPAMQEVFRAIGRLSRSS
ISVLINGQSGTGKELVASALHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDEI
GDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLEQLVYQGGFREDLFHRLNVIRVHLPPLSQRREDIPQL
ARHFLMIAAKEIAVEPKVLTKEAATKLSQLPWPGNVRQLENTCRWLTVMASGQEILPLDLPPELLQEPKLNNAQTSDSDD
WQGALKLFIDQRLSDGDSDLLTEVQPAFERILLQTALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=68.5224839400428, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=40.5117270788913, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1790437, Length=465, Percent_Identity=41.7204301075269, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=38.8412017167382, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=44.578313253012, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=41.3173652694611, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI87082117, Length=335, Percent_Identity=42.6865671641791, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=41.9825072886297, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=40.1812688821752, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082152, Length=247, Percent_Identity=45.748987854251, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI87081872, Length=243, Percent_Identity=46.0905349794239, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081858, Length=317, Percent_Identity=31.5457413249211, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=36.4754098360656, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790552, Length=124, Percent_Identity=29.8387096774194, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52123; Mature: 51992

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQVWILDDDSSIRWVLEKALQSAKFSCASFAAAESLWQALEMTQPQVIVSDIRMPGTD
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GLTLLERLQNHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTH
CHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
AKEQSSSITVEEPLVATPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVASA
HHHHCCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHH
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLEQLVYQGGFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHH
LNVIRVHLPPLSQRREDIPQLARHFLMIAAKEIAVEPKVLTKEAATKLSQLPWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGQEILPLDLPPELLQEPKLNNAQTSDSDDWQGALKLFIDQRLSDGDSD
HHHHHEEEEEECCCEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
LLTEVQPAFERILLQTALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEQVWILDDDSSIRWVLEKALQSAKFSCASFAAAESLWQALEMTQPQVIVSDIRMPGTD
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GLTLLERLQNHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTH
CHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
AKEQSSSITVEEPLVATPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVASA
HHHHCCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHH
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQDLEQLVYQGGFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHH
LNVIRVHLPPLSQRREDIPQLARHFLMIAAKEIAVEPKVLTKEAATKLSQLPWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGQEILPLDLPPELLQEPKLNNAQTSDSDDWQGALKLFIDQRLSDGDSD
HHHHHEEEEEECCCEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
LLTEVQPAFERILLQTALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]