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The map label for this gene is fdhB1 [H]

Identifier: 167622047

GI number: 167622047

Start: 119285

End: 119872

Strand: Direct

Name: fdhB1 [H]

Synonym: Shal_0106

Alternate gene names: 167622047

Gene position: 119285-119872 (Clockwise)

Preceding gene: 167622046

Following gene: 167622048

Centisome position: 2.28

GC content: 45.92

Gene sequence:

>588_bases
ATGGCAAACATGAAATTTTTATGTGATACCAAACGCTGCATCGAGTGTAACGGTTGCGTCACAGCATGTAAAAACGCCAA
TTCTTCAGCTATTGGTTGGGGAATTCAACGTCGTCGCGTGGTTACGCTAAATGACGGTGAGCCGGGTGAAGCATCCATTT
CAGTGGCATGTATGCACTGTACCGATGCACCTTGTATGGCAGTTTGTCCTGCAGATTGTTTTTATCGTACTGATGAAGGC
ATCGTCTTACATAACAAAGAGACCTGTATTGGTTGTGGTTACTGTTTATATGCATGTCCTTTTGGAGCACCTCAGTTCCC
TCAGAAAAATGCATTTGGTAGTCGCGGTAAGATGGACAAATGTACTTTCTGTGCTGGCGGTCCTGCGCCTGATTTCTCGG
AAGAGGAACGTAAGTTATACGGTGCAAACCGTATTGCTGAAGGCAAGCTGCCTTTGTGTGCAGAATTTTGTTCGACTAAG
TCGCTATTAGCGGGAGATGCGTTAGTTATTTCTAATATTTTCCGTGAACGTTCTGCCGCACGCGGCGCACCAGAAGCGCT
TTGGGGTTATAAACCAACAACTGGCTAG

Upstream 100 bases:

>100_bases
TGACTTATGGTTATGATGTTGTGACACAGATGCAAGAGACGAAAGCGAGCTTGTGTCAGGTCAGCAAAGCCTAATTAACT
TGATGAGGAGATAAGCCATT

Downstream 100 bases:

>100_bases
TCTGGCTGAATAGGACTGCAGGCCTTGGCTTGCGGTCCAATGAAGGAGTGAATATGTTTATGAAATCACTGCGCAGCCTA
TTTGCACTGCTAGTAATTCC

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MANMKFLCDTKRCIECNGCVTACKNANSSAIGWGIQRRRVVTLNDGEPGEASISVACMHCTDAPCMAVCPADCFYRTDEG
IVLHNKETCIGCGYCLYACPFGAPQFPQKNAFGSRGKMDKCTFCAGGPAPDFSEEERKLYGANRIAEGKLPLCAEFCSTK
SLLAGDALVISNIFRERSAARGAPEALWGYKPTTG

Sequences:

>Translated_195_residues
MANMKFLCDTKRCIECNGCVTACKNANSSAIGWGIQRRRVVTLNDGEPGEASISVACMHCTDAPCMAVCPADCFYRTDEG
IVLHNKETCIGCGYCLYACPFGAPQFPQKNAFGSRGKMDKCTFCAGGPAPDFSEEERKLYGANRIAEGKLPLCAEFCSTK
SLLAGDALVISNIFRERSAARGAPEALWGYKPTTG
>Mature_194_residues
ANMKFLCDTKRCIECNGCVTACKNANSSAIGWGIQRRRVVTLNDGEPGEASISVACMHCTDAPCMAVCPADCFYRTDEGI
VLHNKETCIGCGYCLYACPFGAPQFPQKNAFGSRGKMDKCTFCAGGPAPDFSEEERKLYGANRIAEGKLPLCAEFCSTKS
LLAGDALVISNIFRERSAARGAPEALWGYKPTTG

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=171, Percent_Identity=32.7485380116959, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787122, Length=194, Percent_Identity=28.8659793814433, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=34.3558282208589, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789370, Length=208, Percent_Identity=26.4423076923077, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790326, Length=193, Percent_Identity=29.0155440414508, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367345, Length=185, Percent_Identity=31.3513513513514, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1787749, Length=207, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789079, Length=104, Percent_Identity=38.4615384615385, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788811, Length=132, Percent_Identity=31.0606060606061, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI87082114, Length=187, Percent_Identity=28.8770053475936, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI2367245, Length=134, Percent_Identity=33.5820895522388, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 20953; Mature: 20822

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
11.8 %Cys+Met (Translated Protein)
9.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
11.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMKFLCDTKRCIECNGCVTACKNANSSAIGWGIQRRRVVTLNDGEPGEASISVACMHC
CCCCCEEECCCCEEECCCHHHECCCCCCCEEECCCCCCEEEEECCCCCCCCEEEEEEEEC
TDAPCMAVCPADCFYRTDEGIVLHNKETCIGCGYCLYACPFGAPQFPQKNAFGSRGKMDK
CCCCEEEECCCHHEEECCCCEEEECCCCEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPAPDFSEEERKLYGANRIAEGKLPLCAEFCSTKSLLAGDALVISNIFRERSAA
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHCCCHHHHHHHHHHHHHC
RGAPEALWGYKPTTG
CCCCHHHCCCCCCCC
>Mature Secondary Structure 
ANMKFLCDTKRCIECNGCVTACKNANSSAIGWGIQRRRVVTLNDGEPGEASISVACMHC
CCCCEEECCCCEEECCCHHHECCCCCCCEEECCCCCCEEEEECCCCCCCCEEEEEEEEC
TDAPCMAVCPADCFYRTDEGIVLHNKETCIGCGYCLYACPFGAPQFPQKNAFGSRGKMDK
CCCCEEEECCCHHEEECCCCEEEECCCCEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPAPDFSEEERKLYGANRIAEGKLPLCAEFCSTKSLLAGDALVISNIFRERSAA
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHCCCHHHHHHHHHHHHHC
RGAPEALWGYKPTTG
CCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]