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The map label for this gene is rlmF

Identifier: 167622038

GI number: 167622038

Start: 109024

End: 110166

Strand: Direct

Name: rlmF

Synonym: Shal_0097

Alternate gene names: 167622038

Gene position: 109024-110166 (Clockwise)

Preceding gene: 167622037

Following gene: 167622041

Centisome position: 2.09

GC content: 45.41

Gene sequence:

>1143_bases
ATGAGTCATAAAACTAAACCTTCTACTCAAGAGCGCAAGGCTGGCAAGCCTTCAGCACCTAAACGAAAAGTGATCTCTAA
ATCGCCAAATTCTAAGCTTAAGACCATCGTTAAGGGGCAGAAAAATACGGCCGTTAAGGCACAAACGTCTAAGCCTCTGC
ACCCGCGAAATGTGCATAATAAGGGTTACGACTTTCCTGCTTTGATCGCTGCCTTTGCCGCGCTAAAATCTTTTGTTAAG
CCTAATCCCTACGGTAATCTATCAATAGACTTTGCCGACCCACAAGCGGTTAAAATGCTAAATGCGGCTCTGTTGAAACT
GCATTATGATGTTGAACACTGGGATATTCCCGCTGGATTTTTATGTCCACCTATTCCCGGCCGTGCCGATTACATTCACT
ACGTCGCAGATCTACTCGCAGTTAAAAAATCCAGCAAAAAGCGAGTACCTAAAGGGCCGAGAGTTAAGGTGTTAGATATT
GGTACTGGTGCCAATGTGATCTACCCGCTGCTCGGAATTCAGTCTTATGGTTGGAGCTTTGTAGGTAGTGATGTAGACCC
ACTCTCTATTGCAAATGCCCAGCAGGTGTTTGCCAGTAATCCAGCGATCGCAGCGAGGTTCAACTCTCGACTACAAACTA
ATGCTAAACATGTATTCCATGGAGTGATTGAGCCAAACGAACGCTTCGATATAACCTTGTGCAACCCACCATTTCACGCT
TCATTAGCAGAAGCGAGTGAGGGCACAGCGCGTAAGCTTAAAAACCTTGCGGCAAATAGAGCTAAATCATCAGAGGCTAA
GCCGTTACGTAGTACAAAGCCTGTTGTGACTAAAACTGAAACGCCCCTTAACTTTGGCGGTCAGAAAGCCGAGTTATGGT
GTGAGGGAGGCGAGCTGCAGTTTTTGCAAACTATGATCAGTGAGAGTCATGAGTTTGCTAGCCAGTGCTTATGGTTCACC
ACGCTAGTGTCTAAAAAGGAAAACCTTAAGCCTGCAAAAGCCTTGTTAGCTAAGGTAAAGGCGGAAGAGGTGAAAGAGAT
GGAGATGCACCAAGGTAATAAGATCACCCGAGTGTTAGCTTGGACCTTCCTTAAGCCTGAACAAAGAGAATTGTGGGCAC
AATATCGAGATGTGGAAAATTAA

Upstream 100 bases:

>100_bases
CCTAGTTGTAAATTTACTCTTTACTAAATATCTATACGATTAATTTTCAATGCAAACATCAGTAGTGTTTGCTCTTTTAC
AGGTGCTAGCGCGTTAAAAC

Downstream 100 bases:

>100_bases
AAAGCTGATGAGCAGCTCATCAGCTTTTCAGTACGCAGTTCTATCTGGTGACGGCATCGAGATTTATGGAAAATGATATT
TCACCAAGTTAGCCAATATA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSHKTKPSTQERKAGKPSAPKRKVISKSPNSKLKTIVKGQKNTAVKAQTSKPLHPRNVHNKGYDFPALIAAFAALKSFVK
PNPYGNLSIDFADPQAVKMLNAALLKLHYDVEHWDIPAGFLCPPIPGRADYIHYVADLLAVKKSSKKRVPKGPRVKVLDI
GTGANVIYPLLGIQSYGWSFVGSDVDPLSIANAQQVFASNPAIAARFNSRLQTNAKHVFHGVIEPNERFDITLCNPPFHA
SLAEASEGTARKLKNLAANRAKSSEAKPLRSTKPVVTKTETPLNFGGQKAELWCEGGELQFLQTMISESHEFASQCLWFT
TLVSKKENLKPAKALLAKVKAEEVKEMEMHQGNKITRVLAWTFLKPEQRELWAQYRDVEN

Sequences:

>Translated_380_residues
MSHKTKPSTQERKAGKPSAPKRKVISKSPNSKLKTIVKGQKNTAVKAQTSKPLHPRNVHNKGYDFPALIAAFAALKSFVK
PNPYGNLSIDFADPQAVKMLNAALLKLHYDVEHWDIPAGFLCPPIPGRADYIHYVADLLAVKKSSKKRVPKGPRVKVLDI
GTGANVIYPLLGIQSYGWSFVGSDVDPLSIANAQQVFASNPAIAARFNSRLQTNAKHVFHGVIEPNERFDITLCNPPFHA
SLAEASEGTARKLKNLAANRAKSSEAKPLRSTKPVVTKTETPLNFGGQKAELWCEGGELQFLQTMISESHEFASQCLWFT
TLVSKKENLKPAKALLAKVKAEEVKEMEMHQGNKITRVLAWTFLKPEQRELWAQYRDVEN
>Mature_379_residues
SHKTKPSTQERKAGKPSAPKRKVISKSPNSKLKTIVKGQKNTAVKAQTSKPLHPRNVHNKGYDFPALIAAFAALKSFVKP
NPYGNLSIDFADPQAVKMLNAALLKLHYDVEHWDIPAGFLCPPIPGRADYIHYVADLLAVKKSSKKRVPKGPRVKVLDIG
TGANVIYPLLGIQSYGWSFVGSDVDPLSIANAQQVFASNPAIAARFNSRLQTNAKHVFHGVIEPNERFDITLCNPPFHAS
LAEASEGTARKLKNLAANRAKSSEAKPLRSTKPVVTKTETPLNFGGQKAELWCEGGELQFLQTMISESHEFASQCLWFTT
LVSKKENLKPAKALLAKVKAEEVKEMEMHQGNKITRVLAWTFLKPEQRELWAQYRDVEN

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=317, Percent_Identity=30.5993690851735, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI87081786, Length=317, Percent_Identity=47.0031545741325, Blast_Score=303, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI32564931, Length=320, Percent_Identity=28.125, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=318, Percent_Identity=27.0440251572327, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281363415, Length=314, Percent_Identity=26.4331210191083, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922302, Length=314, Percent_Identity=26.4331210191083, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_SHEHH (B0TM80)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672332.1
- ProteinModelPortal:   B0TM80
- SMR:   B0TM80
- GeneID:   5903864
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0097
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   SHAL458817:SHAL_0097-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 42108; Mature: 41977

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKTKPSTQERKAGKPSAPKRKVISKSPNSKLKTIVKGQKNTAVKAQTSKPLHPRNVHN
CCCCCCCCCHHHHCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCC
KGYDFPALIAAFAALKSFVKPNPYGNLSIDFADPQAVKMLNAALLKLHYDVEHWDIPAGF
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHEECCHHCCCCCCC
LCPPIPGRADYIHYVADLLAVKKSSKKRVPKGPRVKVLDIGTGANVIYPLLGIQSYGWSF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEE
VGSDVDPLSIANAQQVFASNPAIAARFNSRLQTNAKHVFHGVIEPNERFDITLCNPPFHA
CCCCCCCHHHCCHHHHHHCCCCEEHHHHHHHHHCHHHHHEECCCCCCCEEEEECCCCCCC
SLAEASEGTARKLKNLAANRAKSSEAKPLRSTKPVVTKTETPLNFGGQKAELWCEGGELQ
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCHH
FLQTMISESHEFASQCLWFTTLVSKKENLKPAKALLAKVKAEEVKEMEMHQGNKITRVLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
WTFLKPEQRELWAQYRDVEN
HHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHKTKPSTQERKAGKPSAPKRKVISKSPNSKLKTIVKGQKNTAVKAQTSKPLHPRNVHN
CCCCCCCCHHHHCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCC
KGYDFPALIAAFAALKSFVKPNPYGNLSIDFADPQAVKMLNAALLKLHYDVEHWDIPAGF
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHEECCHHCCCCCCC
LCPPIPGRADYIHYVADLLAVKKSSKKRVPKGPRVKVLDIGTGANVIYPLLGIQSYGWSF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEE
VGSDVDPLSIANAQQVFASNPAIAARFNSRLQTNAKHVFHGVIEPNERFDITLCNPPFHA
CCCCCCCHHHCCHHHHHHCCCCEEHHHHHHHHHCHHHHHEECCCCCCCEEEEECCCCCCC
SLAEASEGTARKLKNLAANRAKSSEAKPLRSTKPVVTKTETPLNFGGQKAELWCEGGELQ
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCHH
FLQTMISESHEFASQCLWFTTLVSKKENLKPAKALLAKVKAEEVKEMEMHQGNKITRVLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
WTFLKPEQRELWAQYRDVEN
HHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA