Click here to switch to the map view.

The map label for this gene is mscM [H]

Identifier: 167622026

GI number: 167622026

Start: 95438

End: 96691

Strand: Direct

Name: mscM [H]

Synonym: Shal_0085

Alternate gene names: 167622026

Gene position: 95438-96691 (Clockwise)

Preceding gene: 167622017

Following gene: 167622029

Centisome position: 1.83

GC content: 44.5

Gene sequence:

>1254_bases
TTGGATCAGGAATTACGCGCTGCGATTGCTCAGTGGTTAACTAATATTGGTATCGACACTCAGCCATCTGATGGTGTTTC
GATTACGATTATGCTGTTTGCATGTTTTGTGGTGGCATTTATCGGGTATTTCTTCGTAAAACGTGGTGTCGTGACCACGA
TGAATCTAGTTATCCTGCGCTCTAAAGCTCATTGGGATGACATTTTCATGCGTCATCAAGTATTGGAGAAGATGGCCGTA
CTTGTCCCCGTTATTATCTTGAATATTCTGTTGCCGTTAGCCTTGTATGATCACACTGTTCTTAGCAGCCTGACTGATCG
ACTACTAGACGTCACTATCGTGGTGTTATTTATCCGTACTATTTACAGCGCCTTAGATGCCGCTAATGAGATTGCGGATT
ATAACTTAGTCAGTCGAAGGTTGCCGATCAAGAGCTTTGTGCAGCTAGCTAAACTGTTCATGTTCTTTGTGGCCATCATC
ATCTCTATTTCAGTTTTGGCAGACCAATCACCCGTCTATTTTCTAAGCGGTTTAGGTGTCGCGACTGGTTTAGTCATGTT
GGTATTTAGAGATACTATCTTAGGCTTCGTTGCGGGTATTCAGTTGGCGGCTAATCGCATGGTGAGTCCTGGAGACTGGA
TCGAGATGGATAACTTCGGTGCCAACGGTACCGTCGAAGAGGTGTCGTTAACCACAGTTAAAGTGCGTAACTTCGACCAA
ACCCTGACCATGCTTCCTGCCTATTCTTTAACTTCAGGGTCCTTTAGAAACTGGCGTGGCATGTCTGAATCAGGCGGTCG
ACGGATTAAGCGCGCGATACAAATTGATATTCAATCGGTGCGTTTTTTAACTGAAGACGATATGCGCAGCTTAAACAAGA
TCAATCACCTTAAAGACTATTTCTCTGCGAAATCGGCGGAGATCATTACATATAATGCCCATGTTGAAGATGCGGATATG
TACGTGAATAGCCGTCATTTGACCAATATCGGTACCTTTAGAGCCTATATCCAGGAGTACTTAACTCAGCACGATAAGAT
CCGTAAAGACATGACACTGATGGTGCGCCAACTTGCACCAACCGAACTGGGTGTACCTATTGAGCTCTATATCTTTACTA
ACGATACACGCTGGGAGTTTTACGAGGGCATTCAAGCTGATATTTTCGATCACCTCTTTGCGATATTGCCAGAGTTTGAT
CTTAGGGTATTCCAAGGGCCAACAGGGGCTGATGTGCGTAGCTTGAAGCATTAG

Upstream 100 bases:

>100_bases
GAGGTTTGTGATGCCTTTGTTTCAACCTTTAGTTGTTTATGTTGGATCTATTTTAATTCGTTGAGTGCTCCCTTGAGCGC
GAAAATATTGGAGAATAGCT

Downstream 100 bases:

>100_bases
TGCGGTGATTGGTATAAGAAGCCAGCTTGAAAGCTGGCTTTTATTTTCAGGCTTGAATCACCTATTTCAGTAGCGAGTTA
TCATCAATTAGGCTTTGCCT

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MDQELRAAIAQWLTNIGIDTQPSDGVSITIMLFACFVVAFIGYFFVKRGVVTTMNLVILRSKAHWDDIFMRHQVLEKMAV
LVPVIILNILLPLALYDHTVLSSLTDRLLDVTIVVLFIRTIYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAII
ISISVLADQSPVYFLSGLGVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNFDQ
TLTMLPAYSLTSGSFRNWRGMSESGGRRIKRAIQIDIQSVRFLTEDDMRSLNKINHLKDYFSAKSAEIITYNAHVEDADM
YVNSRHLTNIGTFRAYIQEYLTQHDKIRKDMTLMVRQLAPTELGVPIELYIFTNDTRWEFYEGIQADIFDHLFAILPEFD
LRVFQGPTGADVRSLKH

Sequences:

>Translated_417_residues
MDQELRAAIAQWLTNIGIDTQPSDGVSITIMLFACFVVAFIGYFFVKRGVVTTMNLVILRSKAHWDDIFMRHQVLEKMAV
LVPVIILNILLPLALYDHTVLSSLTDRLLDVTIVVLFIRTIYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAII
ISISVLADQSPVYFLSGLGVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNFDQ
TLTMLPAYSLTSGSFRNWRGMSESGGRRIKRAIQIDIQSVRFLTEDDMRSLNKINHLKDYFSAKSAEIITYNAHVEDADM
YVNSRHLTNIGTFRAYIQEYLTQHDKIRKDMTLMVRQLAPTELGVPIELYIFTNDTRWEFYEGIQADIFDHLFAILPEFD
LRVFQGPTGADVRSLKH
>Mature_417_residues
MDQELRAAIAQWLTNIGIDTQPSDGVSITIMLFACFVVAFIGYFFVKRGVVTTMNLVILRSKAHWDDIFMRHQVLEKMAV
LVPVIILNILLPLALYDHTVLSSLTDRLLDVTIVVLFIRTIYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAII
ISISVLADQSPVYFLSGLGVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNFDQ
TLTMLPAYSLTSGSFRNWRGMSESGGRRIKRAIQIDIQSVRFLTEDDMRSLNKINHLKDYFSAKSAEIITYNAHVEDADM
YVNSRHLTNIGTFRAYIQEYLTQHDKIRKDMTLMVRQLAPTELGVPIELYIFTNDTRWEFYEGIQADIFDHLFAILPEFD
LRVFQGPTGADVRSLKH

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=393, Percent_Identity=44.7837150127226, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 47261; Mature: 47261

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQELRAAIAQWLTNIGIDTQPSDGVSITIMLFACFVVAFIGYFFVKRGVVTTMNLVILR
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEE
SKAHWDDIFMRHQVLEKMAVLVPVIILNILLPLALYDHTVLSSLTDRLLDVTIVVLFIRT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAIIISISVLADQSPVYFLSGLGV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNFDQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCHH
TLTMLPAYSLTSGSFRNWRGMSESGGRRIKRAIQIDIQSVRFLTEDDMRSLNKINHLKDY
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FSAKSAEIITYNAHVEDADMYVNSRHLTNIGTFRAYIQEYLTQHDKIRKDMTLMVRQLAP
HCCCCCEEEEEECCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TELGVPIELYIFTNDTRWEFYEGIQADIFDHLFAILPEFDLRVFQGPTGADVRSLKH
CCCCCCEEEEEEECCCCHHHHHCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHCCC
>Mature Secondary Structure
MDQELRAAIAQWLTNIGIDTQPSDGVSITIMLFACFVVAFIGYFFVKRGVVTTMNLVILR
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEE
SKAHWDDIFMRHQVLEKMAVLVPVIILNILLPLALYDHTVLSSLTDRLLDVTIVVLFIRT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAIIISISVLADQSPVYFLSGLGV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNFDQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCHH
TLTMLPAYSLTSGSFRNWRGMSESGGRRIKRAIQIDIQSVRFLTEDDMRSLNKINHLKDY
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FSAKSAEIITYNAHVEDADMYVNSRHLTNIGTFRAYIQEYLTQHDKIRKDMTLMVRQLAP
HCCCCCEEEEEECCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TELGVPIELYIFTNDTRWEFYEGIQADIFDHLFAILPEFDLRVFQGPTGADVRSLKH
CCCCCCEEEEEEECCCCHHHHHCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]