Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 16752300

GI number: 16752300

Start: 3235

End: 5403

Strand: Direct

Name: Not Available

Synonym: CP0004

Alternate gene names: 16752300

Gene position: 3235-5403 (Clockwise)

Preceding gene: 16752297

Following gene: 16752301

Centisome position: 0.26

GC content: 39.51

Gene sequence:

>2169_bases
GTGGACTATTTAGAAAAGTTGCAAGTCTTAATAGAAGAAGGCCAATCTGCGAACTTTTTAAGCCTTTGGGAGGAGTATTG
TTTTAACGATGTAGTGCGGGGGCGGGAGCTTGTTGAGATCCTAGAAAAAGTAAAGTCTTCCTCTTTAGCATCTTTATTTG
GAAAGATTGTGGATACCGTCGTTCCTCTTTGGGAAAAAATACCCGAGGGAAAGGATAAAGATCGAGTTCTTCAATTAATT
TTAGATTTGCAAACATCTAACAGCCAGATGTTTTTTGATATAGCCACTGAATATGTGAATAAAAAGTACAGTGGAGAAGA
AAACTTTAACGAGGCTTTGCGTGTTGTTGGGCTTCGTGATGGTCGTGATTTTCAGTTTAGCCTCAGCCGTTTTGATTTTT
TGATGCATATGCATAAGGGAAACTTTGTCTTCCATCAAGGGGGTTGGGGTGTTGGCGAGGTTATGGGGGTCTCGTTTCTT
CAGCAGAAGGTGTTGATAGAGTTTGAAGGAATTATGAGTGCCAAGGATATTTCTTTCGAGACGGCATTTAAAAGTTTAAC
TCCTTTGAGCGGCGATCACTTCCTTTCACGACGGTTTGGAGATCCCGATGGGTTTGAGGCTTTTGCTAAGGAAAACCCTA
TTGAAGTTGTTGAGATTCTCCTTAGAGATCTTGGACCAAAGACCGCAAAGGAAATCAAAGACGAGTTAGTTGATCTTGTG
ATTCCTGAGGCAGATTGGAATCGGTGGTGGCAATCAGCTAAGACTAAGATTAAAAAGGGAACTAGAATTATATCCCCAGA
CAATCCTAAAGAGCCCTATGTTTTATCTGATGCAGGGTGTTCTCATATGGGACAGTTGGAGCGTAAGTTGGGATTGAGTC
TCAATAGCGCAGAAAAGATCTCTCTGATCTATCATTTTATTAGAGATTTGCATAGTGAATTGAAAAATATAGAGATTCGC
AAGAGTCTAGTTAAAGCTTTGCAAGATCTTGATGTTGAGGAAGGTAATAAGTCTTTAATTTTGCAGAGAGAACTGCTTCT
TTCTGAGTATTTGGGAATTAAGGATGCGAGCATAGACAAAGAGTATATAACATCCTTATCAGAAGATGATACCAGTCGTC
TTTTGGAAAACATGCCTATTGTTGCTTTGCAAAAGTCCTTTTTGTCTTTGGTAAGAAAGTACTCTTCCTTCTGGCAACAA
GTTTTTATGCAAATTCTTCTTTATACCACCTCTCCAACCATGAGGGATTTTGTATATAAAACCATTAAAAACGATCCATC
AAGCGTAGAGGTTTTAAAGAAAAGACTTCTAGATAGTGCCCATCAACCAATGATGTTCCCTGAGCTGTTTGTTTGGTTTT
TCTTGAAGCTTGGGAACCATGAAGATGGTCTCTTTGATCCTGAAGACAAGGAAGTCTTAAGATTGTTCTTAGAGTCAGCT
TTGAATTTTATGTATCAAGTAGCCTCTACACCTCATAAAGAACTGGGAAAAAAGTTACACCACTACTTGGTGGGGCAAAG
GTATCTCGCTGTGCGTCAGATGATAGAAGGCGCATCCCTTCCCTTTTTAAAAGAATTACTATTACTATCCACCAAATGCC
CGCAGTTTTCTTCTTCTGATTTGAACGTTTTACAAAGCCTTGCTGAAGTTGTTCAGCCAACGCTAAAAAAGCACAAGTCT
AATGTAGAAGAAGAAAATGTTTTATGGTCGACTTCTGAGAGTTTTTCAAGAATGAAAGCCAAGCTACAATCTCTTGTTGG
CAAGGAGATGGTTGATAATGCTAAGGAAATTGAAGATGCTCGTTCTTTAGGGGATTTGCGGGAAAATTCTGAGTACAAGT
TTGCTTTAGAGAAGAGAGCTCGCTTACAAGAAGAAATTCGCGTGCTATCGGAAGAAATTAATCGGGCGAGAATTCTTACA
AAAGACCTTGTCTTTACAGATAAAGTTGGTGTTGGCTGTAAAGTTACTTTAAAAGGGGATGCTGGAGAGGTTGTGGAGTA
TACAATTTTAGGACCTTGGGATGCCGATCCAGATAGTTGTATACTTTCTTTACAATCCAAGCTTGCGCAGAACATGTTAG
GGAAGAAGTTGAATGACGTGGTAATACTGCAAGGAAAGGAGTATAAGATTTCTCGGATACAATCCATTTGGGAAGAACAT
GGAGCATAG

Upstream 100 bases:

>100_bases
CATTTTAGTTTATTATAAAATAAGTTTGATTATTTTGTATTGTGATATTGCTCTTTTTCATACGTAAGAGTATATTTTTA
GGTTAAAAACTGGAGACATC

Downstream 100 bases:

>100_bases
CGGGGTCGAACCGCTGACCTCAACAATGCCATTGTTGCGCTCTACCAACTGAGCTAATACCCCAAAAGATACAAAAAAGT
ATCATGTTCCAGGGTTTAGA

Product: transcript cleavage factor

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MDYLEKLQVLIEEGQSANFLSLWEEYCFNDVVRGRELVEILEKVKSSSLASLFGKIVDTVVPLWEKIPEGKDKDRVLQLI
LDLQTSNSQMFFDIATEYVNKKYSGEENFNEALRVVGLRDGRDFQFSLSRFDFLMHMHKGNFVFHQGGWGVGEVMGVSFL
QQKVLIEFEGIMSAKDISFETAFKSLTPLSGDHFLSRRFGDPDGFEAFAKENPIEVVEILLRDLGPKTAKEIKDELVDLV
IPEADWNRWWQSAKTKIKKGTRIISPDNPKEPYVLSDAGCSHMGQLERKLGLSLNSAEKISLIYHFIRDLHSELKNIEIR
KSLVKALQDLDVEEGNKSLILQRELLLSEYLGIKDASIDKEYITSLSEDDTSRLLENMPIVALQKSFLSLVRKYSSFWQQ
VFMQILLYTTSPTMRDFVYKTIKNDPSSVEVLKKRLLDSAHQPMMFPELFVWFFLKLGNHEDGLFDPEDKEVLRLFLESA
LNFMYQVASTPHKELGKKLHHYLVGQRYLAVRQMIEGASLPFLKELLLLSTKCPQFSSSDLNVLQSLAEVVQPTLKKHKS
NVEEENVLWSTSESFSRMKAKLQSLVGKEMVDNAKEIEDARSLGDLRENSEYKFALEKRARLQEEIRVLSEEINRARILT
KDLVFTDKVGVGCKVTLKGDAGEVVEYTILGPWDADPDSCILSLQSKLAQNMLGKKLNDVVILQGKEYKISRIQSIWEEH
GA

Sequences:

>Translated_722_residues
MDYLEKLQVLIEEGQSANFLSLWEEYCFNDVVRGRELVEILEKVKSSSLASLFGKIVDTVVPLWEKIPEGKDKDRVLQLI
LDLQTSNSQMFFDIATEYVNKKYSGEENFNEALRVVGLRDGRDFQFSLSRFDFLMHMHKGNFVFHQGGWGVGEVMGVSFL
QQKVLIEFEGIMSAKDISFETAFKSLTPLSGDHFLSRRFGDPDGFEAFAKENPIEVVEILLRDLGPKTAKEIKDELVDLV
IPEADWNRWWQSAKTKIKKGTRIISPDNPKEPYVLSDAGCSHMGQLERKLGLSLNSAEKISLIYHFIRDLHSELKNIEIR
KSLVKALQDLDVEEGNKSLILQRELLLSEYLGIKDASIDKEYITSLSEDDTSRLLENMPIVALQKSFLSLVRKYSSFWQQ
VFMQILLYTTSPTMRDFVYKTIKNDPSSVEVLKKRLLDSAHQPMMFPELFVWFFLKLGNHEDGLFDPEDKEVLRLFLESA
LNFMYQVASTPHKELGKKLHHYLVGQRYLAVRQMIEGASLPFLKELLLLSTKCPQFSSSDLNVLQSLAEVVQPTLKKHKS
NVEEENVLWSTSESFSRMKAKLQSLVGKEMVDNAKEIEDARSLGDLRENSEYKFALEKRARLQEEIRVLSEEINRARILT
KDLVFTDKVGVGCKVTLKGDAGEVVEYTILGPWDADPDSCILSLQSKLAQNMLGKKLNDVVILQGKEYKISRIQSIWEEH
GA
>Mature_722_residues
MDYLEKLQVLIEEGQSANFLSLWEEYCFNDVVRGRELVEILEKVKSSSLASLFGKIVDTVVPLWEKIPEGKDKDRVLQLI
LDLQTSNSQMFFDIATEYVNKKYSGEENFNEALRVVGLRDGRDFQFSLSRFDFLMHMHKGNFVFHQGGWGVGEVMGVSFL
QQKVLIEFEGIMSAKDISFETAFKSLTPLSGDHFLSRRFGDPDGFEAFAKENPIEVVEILLRDLGPKTAKEIKDELVDLV
IPEADWNRWWQSAKTKIKKGTRIISPDNPKEPYVLSDAGCSHMGQLERKLGLSLNSAEKISLIYHFIRDLHSELKNIEIR
KSLVKALQDLDVEEGNKSLILQRELLLSEYLGIKDASIDKEYITSLSEDDTSRLLENMPIVALQKSFLSLVRKYSSFWQQ
VFMQILLYTTSPTMRDFVYKTIKNDPSSVEVLKKRLLDSAHQPMMFPELFVWFFLKLGNHEDGLFDPEDKEVLRLFLESA
LNFMYQVASTPHKELGKKLHHYLVGQRYLAVRQMIEGASLPFLKELLLLSTKCPQFSSSDLNVLQSLAEVVQPTLKKHKS
NVEEENVLWSTSESFSRMKAKLQSLVGKEMVDNAKEIEDARSLGDLRENSEYKFALEKRARLQEEIRVLSEEINRARILT
KDLVFTDKVGVGCKVTLKGDAGEVVEYTILGPWDADPDSCILSLQSKLAQNMLGKKLNDVVILQGKEYKISRIQSIWEEH
GA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG1747

COG function: function code S; Uncharacterized N-terminal domain of the transcription elongation factor GreA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GRAD1 (greA associated 1) domain

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_CHLPN (Q9Z7G4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86583
- PIR:   C72040
- RefSeq:   NP_224937.1
- RefSeq:   NP_300797.1
- RefSeq:   NP_444557.1
- RefSeq:   NP_877041.1
- ProteinModelPortal:   Q9Z7G4
- SMR:   Q9Z7G4
- GeneID:   1467448
- GeneID:   895546
- GeneID:   919509
- GeneID:   963627
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0004
- KEGG:   cpn:CPn0741
- KEGG:   cpt:CpB0769
- TIGR:   CP_0004
- HOGENOM:   HBG402689
- OMA:   EYKFALE
- ProtClustDB:   PRK06330
- BioCyc:   CPNE115711:CP_0004-MONOMER
- BioCyc:   CPNE115713:CPN0741-MONOMER
- BioCyc:   CPNE138677:CPJ0741-MONOMER
- BioCyc:   CPNE182082:CPB0769-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 82643; Mature: 82643

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLEKLQVLIEEGQSANFLSLWEEYCFNDVVRGRELVEILEKVKSSSLASLFGKIVDTV
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPLWEKIPEGKDKDRVLQLILDLQTSNSQMFFDIATEYVNKKYSGEENFNEALRVVGLRD
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
GRDFQFSLSRFDFLMHMHKGNFVFHQGGWGVGEVMGVSFLQQKVLIEFEGIMSAKDISFE
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TAFKSLTPLSGDHFLSRRFGDPDGFEAFAKENPIEVVEILLRDLGPKTAKEIKDELVDLV
HHHHHCCCCCCCHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IPEADWNRWWQSAKTKIKKGTRIISPDNPKEPYVLSDAGCSHMGQLERKLGLSLNSAEKI
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHH
SLIYHFIRDLHSELKNIEIRKSLVKALQDLDVEEGNKSLILQRELLLSEYLGIKDASIDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
EYITSLSEDDTSRLLENMPIVALQKSFLSLVRKYSSFWQQVFMQILLYTTSPTMRDFVYK
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TIKNDPSSVEVLKKRLLDSAHQPMMFPELFVWFFLKLGNHEDGLFDPEDKEVLRLFLESA
HHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LNFMYQVASTPHKELGKKLHHYLVGQRYLAVRQMIEGASLPFLKELLLLSTKCPQFSSSD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCH
LNVLQSLAEVVQPTLKKHKSNVEEENVLWSTSESFSRMKAKLQSLVGKEMVDNAKEIEDA
HHHHHHHHHHHHHHHHHHHCCCCHHHCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSLGDLRENSEYKFALEKRARLQEEIRVLSEEINRARILTKDLVFTDKVGVGCKVTLKGD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCC
AGEVVEYTILGPWDADPDSCILSLQSKLAQNMLGKKLNDVVILQGKEYKISRIQSIWEEH
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
GA
CC
>Mature Secondary Structure
MDYLEKLQVLIEEGQSANFLSLWEEYCFNDVVRGRELVEILEKVKSSSLASLFGKIVDTV
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPLWEKIPEGKDKDRVLQLILDLQTSNSQMFFDIATEYVNKKYSGEENFNEALRVVGLRD
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
GRDFQFSLSRFDFLMHMHKGNFVFHQGGWGVGEVMGVSFLQQKVLIEFEGIMSAKDISFE
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TAFKSLTPLSGDHFLSRRFGDPDGFEAFAKENPIEVVEILLRDLGPKTAKEIKDELVDLV
HHHHHCCCCCCCHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IPEADWNRWWQSAKTKIKKGTRIISPDNPKEPYVLSDAGCSHMGQLERKLGLSLNSAEKI
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHH
SLIYHFIRDLHSELKNIEIRKSLVKALQDLDVEEGNKSLILQRELLLSEYLGIKDASIDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
EYITSLSEDDTSRLLENMPIVALQKSFLSLVRKYSSFWQQVFMQILLYTTSPTMRDFVYK
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TIKNDPSSVEVLKKRLLDSAHQPMMFPELFVWFFLKLGNHEDGLFDPEDKEVLRLFLESA
HHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LNFMYQVASTPHKELGKKLHHYLVGQRYLAVRQMIEGASLPFLKELLLLSTKCPQFSSSD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCH
LNVLQSLAEVVQPTLKKHKSNVEEENVLWSTSESFSRMKAKLQSLVGKEMVDNAKEIEDA
HHHHHHHHHHHHHHHHHHHCCCCHHHCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSLGDLRENSEYKFALEKRARLQEEIRVLSEEINRARILTKDLVFTDKVGVGCKVTLKGD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCC
AGEVVEYTILGPWDADPDSCILSLQSKLAQNMLGKKLNDVVILQGKEYKISRIQSIWEEH
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362