Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 167041019

GI number: 167041019

Start: 2453480

End: 2454862

Strand: Reverse

Name: trmE [H]

Synonym: Teth514_2409

Alternate gene names: 167041019

Gene position: 2454862-2453480 (Counterclockwise)

Preceding gene: 167041020

Following gene: 167041018

Centisome position: 99.9

GC content: 32.47

Gene sequence:

>1383_bases
ATGGTTTTTGATACAATTGCAGCTATTTCCACTTTTCCAGGGGAAGCAGGAATTGGAATAGTTAGATTAAGTGGAGATGA
TGCTTTAGAAATAATTTCAAAAATTTTTAAACCCTATAAGTCTAAAGATATAAAAAAAGTGAAAAGTCATACTTTACATT
ATGGACATATAGTAGACCCTGAAACAGAAGAAGTTTATGATGAAGTTTTGGTATCTATTATGGAAAAACCTAATACTTAT
ACGAGGGAAGATATAGTTGAAATAAATTGTCATGGAGGTATAGTAGTTACTTCCAAAATTCTTGAACTAGTATTAAAGCA
AGGAGCGAGACTAGCGGAGCCAGGAGAATTTACAAAGAGGGCTTTCTTAAACGGAAGAATTGATTTGTCTCAGGCAGAAG
CGGTCATAGATATAATAAGGGCAAAAACTATGCTTGCTAATAGATATGCTCAGAAACAGTTGGTTGGATATGTTGGAAGT
AAAATAAAGGAAATGAAAGATAAAATCATGGGTCTATTAGTCCATCTTTTAGCTTTAATAGATTTTCCTGAAGAAGATGT
GGAAGAACTTGAGAGAAAAGAAATATTAGAAACTGCTAAGGAAATAGTAGAAGATATCGATAAACTCATTGCATCTTCTG
AAAGTGGAAGGATTATAAGAGAAGGACTTAAAACTGCCATAATTGGTAAACCAAATGTGGGTAAATCTTCTCTTTTAAAT
GCCTTATTAAAAGAAAATAGAGCCATTGTCACAGATATACCAGGAACTACCAGAGACATTATTGAAGAATATGTTAATGT
AAAAGGTATTCCAATAAAGCTTATTGATACAGCAGGAATTAGAGATACAGATGAATTGGTGGAAAAAATAGGGGTTACTA
AAAGCAAAGAAGTTCTGGCTGAAGCAGATTTAATATTGTTTGTGCTGGATGCCTCAAGAGAGTTGACTAAAGAAGACTAC
GAAATTTTTGACATTTTAACAGGTAAAAACATCATATTTGTGTTGAATAAGATAGATCTACCTAAAAAAATTGATGAAAA
AGAATTAAAAGATCTTACCAAAGATGGTATAATAATAGAGGTTTCGACTGTTGAAAAAATTGGATTAGAAGAATTAGAAA
ACACTATTTATAATTTGGTGTTTAGAGGAGACATAAGTTTGAGGGAAGATGAAATAGTCATAAATTCGCGGCACAAAGAA
GCGCTCATTAATGCTAAAAAGTATATGGAAAGCTGTGTTGAAGCTATTGAAGGGGGGTATAGTGAAGACCTAATTACAAT
TGATTTAAATGCGGCTCTTGACCAATTAGGTAAAATTACTGGAGAGACGGCAACTGAGGATTTAATAAATGAAATTTTTG
AGAGATTTTGTGTGGGAAAGTAG

Upstream 100 bases:

>100_bases
AAAATAATACCCTGTCAAGGGTTTTTTTTATATTTCTGATTGAAAAAGTGAAAACATTGGTATATTATTTTATAAGACAA
TTTTTTGGTTGGTGATGAAA

Downstream 100 bases:

>100_bases
GTGAAAGTATGAATTTTATTGCTGGTGAATACGATGTTGCAGTTGTTGGGTTGGGACATGCGGGCAGTGAGGCTGCTCTC
GCTTGTGCAAGGTTGGGACT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MVFDTIAAISTFPGEAGIGIVRLSGDDALEIISKIFKPYKSKDIKKVKSHTLHYGHIVDPETEEVYDEVLVSIMEKPNTY
TREDIVEINCHGGIVVTSKILELVLKQGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTMLANRYAQKQLVGYVGS
KIKEMKDKIMGLLVHLLALIDFPEEDVEELERKEILETAKEIVEDIDKLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
ALLKENRAIVTDIPGTTRDIIEEYVNVKGIPIKLIDTAGIRDTDELVEKIGVTKSKEVLAEADLILFVLDASRELTKEDY
EIFDILTGKNIIFVLNKIDLPKKIDEKELKDLTKDGIIIEVSTVEKIGLEELENTIYNLVFRGDISLREDEIVINSRHKE
ALINAKKYMESCVEAIEGGYSEDLITIDLNAALDQLGKITGETATEDLINEIFERFCVGK

Sequences:

>Translated_460_residues
MVFDTIAAISTFPGEAGIGIVRLSGDDALEIISKIFKPYKSKDIKKVKSHTLHYGHIVDPETEEVYDEVLVSIMEKPNTY
TREDIVEINCHGGIVVTSKILELVLKQGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTMLANRYAQKQLVGYVGS
KIKEMKDKIMGLLVHLLALIDFPEEDVEELERKEILETAKEIVEDIDKLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
ALLKENRAIVTDIPGTTRDIIEEYVNVKGIPIKLIDTAGIRDTDELVEKIGVTKSKEVLAEADLILFVLDASRELTKEDY
EIFDILTGKNIIFVLNKIDLPKKIDEKELKDLTKDGIIIEVSTVEKIGLEELENTIYNLVFRGDISLREDEIVINSRHKE
ALINAKKYMESCVEAIEGGYSEDLITIDLNAALDQLGKITGETATEDLINEIFERFCVGK
>Mature_460_residues
MVFDTIAAISTFPGEAGIGIVRLSGDDALEIISKIFKPYKSKDIKKVKSHTLHYGHIVDPETEEVYDEVLVSIMEKPNTY
TREDIVEINCHGGIVVTSKILELVLKQGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRAKTMLANRYAQKQLVGYVGS
KIKEMKDKIMGLLVHLLALIDFPEEDVEELERKEILETAKEIVEDIDKLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
ALLKENRAIVTDIPGTTRDIIEEYVNVKGIPIKLIDTAGIRDTDELVEKIGVTKSKEVLAEADLILFVLDASRELTKEDY
EIFDILTGKNIIFVLNKIDLPKKIDEKELKDLTKDGIIIEVSTVEKIGLEELENTIYNLVFRGDISLREDEIVINSRHKE
ALINAKKYMESCVEAIEGGYSEDLITIDLNAALDQLGKITGETATEDLINEIFERFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=477, Percent_Identity=32.0754716981132, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=30.1775147928994, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=31.1158798283262, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI2367268, Length=465, Percent_Identity=33.3333333333333, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=39.344262295082, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=470, Percent_Identity=27.8723404255319, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.4259634888438, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45550813, Length=489, Percent_Identity=31.0838445807771, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51340; Mature: 51340

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFDTIAAISTFPGEAGIGIVRLSGDDALEIISKIFKPYKSKDIKKVKSHTLHYGHIVDP
CCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCC
ETEEVYDEVLVSIMEKPNTYTREDIVEINCHGGIVVTSKILELVLKQGARLAEPGEFTKR
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIIRAKTMLANRYAQKQLVGYVGSKIKEMKDKIMGLLVHLLALI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFPEEDVEELERKEILETAKEIVEDIDKLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCCCCHHHHHH
ALLKENRAIVTDIPGTTRDIIEEYVNVKGIPIKLIDTAGIRDTDELVEKIGVTKSKEVLA
HHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHH
EADLILFVLDASRELTKEDYEIFDILTGKNIIFVLNKIDLPKKIDEKELKDLTKDGIIIE
HCCEEEEEECCCHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCHHHHHHHHHCCEEEE
VSTVEKIGLEELENTIYNLVFRGDISLREDEIVINSRHKEALINAKKYMESCVEAIEGGY
ECCHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCC
SEDLITIDLNAALDQLGKITGETATEDLINEIFERFCVGK
CCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVFDTIAAISTFPGEAGIGIVRLSGDDALEIISKIFKPYKSKDIKKVKSHTLHYGHIVDP
CCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCC
ETEEVYDEVLVSIMEKPNTYTREDIVEINCHGGIVVTSKILELVLKQGARLAEPGEFTKR
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIIRAKTMLANRYAQKQLVGYVGSKIKEMKDKIMGLLVHLLALI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFPEEDVEELERKEILETAKEIVEDIDKLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCCCCHHHHHH
ALLKENRAIVTDIPGTTRDIIEEYVNVKGIPIKLIDTAGIRDTDELVEKIGVTKSKEVLA
HHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHH
EADLILFVLDASRELTKEDYEIFDILTGKNIIFVLNKIDLPKKIDEKELKDLTKDGIIIE
HCCEEEEEECCCHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCHHHHHHHHHCCEEEE
VSTVEKIGLEELENTIYNLVFRGDISLREDEIVINSRHKEALINAKKYMESCVEAIEGGY
ECCHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCC
SEDLITIDLNAALDQLGKITGETATEDLINEIFERFCVGK
CCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA