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The map label for this gene is levR [H]

Identifier: 167040814

GI number: 167040814

Start: 2189191

End: 2190504

Strand: Reverse

Name: levR [H]

Synonym: Teth514_2191

Alternate gene names: 167040814

Gene position: 2190504-2189191 (Counterclockwise)

Preceding gene: 167040816

Following gene: 167040813

Centisome position: 89.14

GC content: 31.89

Gene sequence:

>1314_bases
ATGTTTTATATGACTAATAAAGAAAAAATTTACAATTTCATTATAGAAAAGAGTAAGGAATTTAATCTTCAGGATTTTTT
GATAAATAGAAAAGGTGGGGTCACTACAGAAGAAATAGCGCAATTGCTAAAAATAGCTCGACCCAATGTGTCTTTTATTT
TAAATCATCTTGTAAGAGAAGGGAAAATAGTTAAAATTATTTCAAAACCTGTGTATTATATTGAAAAAAATAGATTAGCT
CATTTTTTAGGGAATAAGGTTAAGGAAGAGATACTTCTAAAAGATTTAATTGATTTTTTAGATTTGGGAAAAGAAAAAGA
ATGTGCTTTTGAAAATATAATAGGACATGATGGCAGCCTCAAAGGGCAAATAGAACAGGCCAAAGCGGCTGTATTGTATC
CTCCTTATGGACTTCATACTCTTATCATAGGACCGCCGGGTTCTGGTAAATCTTTTTTCGCAGAGATGATGCACAAATTT
AGTCTTCAAAATGGTAGGGTAGGACCTCTTGAAGTGTTAAACTGTGCGGATTACTATAACAATCCTCAACTTCTTATGTC
TTATTTGTTTGGACATGTTAAAGGAGCCTATACTGGAGCTGATGTAGATAAGATAGGTTTAGTAGAGAAAGCTGATAAAG
GGATATTATTCCTTGACGAGGTTCACAGATTACCTCCTGAAGGTCAAGAGATGTTATTTTACCTTATGGACAAGGGAGTA
TATCGAAAATTGGGGGATACTGTGGAAAGAAATGCTAACTTAATGATTGTAGCAGCTACTACAGAGAGACCTGATTCATT
TCTTTTAAAGACTTTTATGAGACGAATACCAGTTATCATTCAATTGCCATCTTTTGAGGAAAGGCCTCTAAACGAGAAAA
TTGAGATAATTGTTTACCTTTTTAGAGAAGAAGCCAAACGGCTCAACTTAAATATTGTTGTACATCCAGAAGTAATTTCT
TTGCTTTTGTCTTTGCAATATGAAGGAAATATTGGGGAATTGAAATCCCTCCTTCAATTAATTTGCTCTAAAGCTTTTTT
AATTAATTACGGAACAAAGAGGAATTTTTTAAAAGTTGATCTTACTTTGTTACCCCAGGCTTATTTGAAAGTAGGGCGGA
ATGATAGAGCAACTTTTGATAAATTTTTAATCATTTCTCCTGAAAAAGAAAAGAAAATAGATAGATATGATTTTTATGAA
TTTATCGCTATGAAATATCATGAATTAAAAGAAAAGGAAATTGAGGGAATATTATTTGAAAAGGGAATAATCGATTGTAT
TAATAACTTTTTAGAAAGCATTTTGGAGGGATAA

Upstream 100 bases:

>100_bases
CCACGGGTCTAAAGCGATAGTTTTGCTATGATAGCCGGGCTGCCAACATTTATATATAATCTCCTGCCTTTTGACAGGAG
GTTTATTAATTGAGGAAGTG

Downstream 100 bases:

>100_bases
TTTTAGAAAAAATGGGAATCTTCTATAGATTAGTATATCTAAGAATATATCACTATAATATATCATTCTAGAGATATTTT
TAATAAATGGAGGATTATCG

Product: sigma-54 factor interaction domain-containing protein

Products: NA

Alternate protein names: Putative phosphotransferase EIIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MFYMTNKEKIYNFIIEKSKEFNLQDFLINRKGGVTTEEIAQLLKIARPNVSFILNHLVREGKIVKIISKPVYYIEKNRLA
HFLGNKVKEEILLKDLIDFLDLGKEKECAFENIIGHDGSLKGQIEQAKAAVLYPPYGLHTLIIGPPGSGKSFFAEMMHKF
SLQNGRVGPLEVLNCADYYNNPQLLMSYLFGHVKGAYTGADVDKIGLVEKADKGILFLDEVHRLPPEGQEMLFYLMDKGV
YRKLGDTVERNANLMIVAATTERPDSFLLKTFMRRIPVIIQLPSFEERPLNEKIEIIVYLFREEAKRLNLNIVVHPEVIS
LLLSLQYEGNIGELKSLLQLICSKAFLINYGTKRNFLKVDLTLLPQAYLKVGRNDRATFDKFLIISPEKEKKIDRYDFYE
FIAMKYHELKEKEIEGILFEKGIIDCINNFLESILEG

Sequences:

>Translated_437_residues
MFYMTNKEKIYNFIIEKSKEFNLQDFLINRKGGVTTEEIAQLLKIARPNVSFILNHLVREGKIVKIISKPVYYIEKNRLA
HFLGNKVKEEILLKDLIDFLDLGKEKECAFENIIGHDGSLKGQIEQAKAAVLYPPYGLHTLIIGPPGSGKSFFAEMMHKF
SLQNGRVGPLEVLNCADYYNNPQLLMSYLFGHVKGAYTGADVDKIGLVEKADKGILFLDEVHRLPPEGQEMLFYLMDKGV
YRKLGDTVERNANLMIVAATTERPDSFLLKTFMRRIPVIIQLPSFEERPLNEKIEIIVYLFREEAKRLNLNIVVHPEVIS
LLLSLQYEGNIGELKSLLQLICSKAFLINYGTKRNFLKVDLTLLPQAYLKVGRNDRATFDKFLIISPEKEKKIDRYDFYE
FIAMKYHELKEKEIEGILFEKGIIDCINNFLESILEG
>Mature_437_residues
MFYMTNKEKIYNFIIEKSKEFNLQDFLINRKGGVTTEEIAQLLKIARPNVSFILNHLVREGKIVKIISKPVYYIEKNRLA
HFLGNKVKEEILLKDLIDFLDLGKEKECAFENIIGHDGSLKGQIEQAKAAVLYPPYGLHTLIIGPPGSGKSFFAEMMHKF
SLQNGRVGPLEVLNCADYYNNPQLLMSYLFGHVKGAYTGADVDKIGLVEKADKGILFLDEVHRLPPEGQEMLFYLMDKGV
YRKLGDTVERNANLMIVAATTERPDSFLLKTFMRRIPVIIQLPSFEERPLNEKIEIIVYLFREEAKRLNLNIVVHPEVIS
LLLSLQYEGNIGELKSLLQLICSKAFLINYGTKRNFLKVDLTLLPQAYLKVGRNDRATFDKFLIISPEKEKKIDRYDFYE
FIAMKYHELKEKEIEGILFEKGIIDCINNFLESILEG

Specific function: Involved in positive regulation of the levanase operon which comprises the levDEFG genes for a fructose PTS system, and sacA for levanase [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=31.3492063492063, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789087, Length=308, Percent_Identity=29.5454545454545, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082117, Length=224, Percent_Identity=33.0357142857143, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788905, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790437, Length=217, Percent_Identity=31.3364055299539, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790299, Length=216, Percent_Identity=31.0185185185185, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788550, Length=219, Percent_Identity=30.5936073059361, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87081872, Length=239, Percent_Identity=30.9623430962343, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=28.8793103448276, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082152, Length=206, Percent_Identity=31.5533980582524, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011608
- InterPro:   IPR004701
- InterPro:   IPR002078
- InterPro:   IPR001550 [H]

Pfam domain/function: PF03610 EIIA-man; PF00874 PRD; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50287; Mature: 50287

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00690 DEAH_ATP_HELICASE ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYMTNKEKIYNFIIEKSKEFNLQDFLINRKGGVTTEEIAQLLKIARPNVSFILNHLVRE
CCEECCHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
GKIVKIISKPVYYIEKNRLAHFLGNKVKEEILLKDLIDFLDLGKEKECAFENIIGHDGSL
CCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
KGQIEQAKAAVLYPPYGLHTLIIGPPGSGKSFFAEMMHKFSLQNGRVGPLEVLNCADYYN
CCHHHHCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
NPQLLMSYLFGHVKGAYTGADVDKIGLVEKADKGILFLDEVHRLPPEGQEMLFYLMDKGV
CHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCHH
YRKLGDTVERNANLMIVAATTERPDSFLLKTFMRRIPVIIQLPSFEERPLNEKIEIIVYL
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHE
FREEAKRLNLNIVVHPEVISLLLSLQYEGNIGELKSLLQLICSKAFLINYGTKRNFLKVD
EHHHHHHCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEE
LTLLPQAYLKVGRNDRATFDKFLIISPEKEKKIDRYDFYEFIAMKYHELKEKEIEGILFE
HHHHCHHHHHCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIIDCINNFLESILEG
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFYMTNKEKIYNFIIEKSKEFNLQDFLINRKGGVTTEEIAQLLKIARPNVSFILNHLVRE
CCEECCHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
GKIVKIISKPVYYIEKNRLAHFLGNKVKEEILLKDLIDFLDLGKEKECAFENIIGHDGSL
CCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
KGQIEQAKAAVLYPPYGLHTLIIGPPGSGKSFFAEMMHKFSLQNGRVGPLEVLNCADYYN
CCHHHHCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
NPQLLMSYLFGHVKGAYTGADVDKIGLVEKADKGILFLDEVHRLPPEGQEMLFYLMDKGV
CHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCHH
YRKLGDTVERNANLMIVAATTERPDSFLLKTFMRRIPVIIQLPSFEERPLNEKIEIIVYL
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHE
FREEAKRLNLNIVVHPEVISLLLSLQYEGNIGELKSLLQLICSKAFLINYGTKRNFLKVD
EHHHHHHCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEE
LTLLPQAYLKVGRNDRATFDKFLIISPEKEKKIDRYDFYEFIAMKYHELKEKEIEGILFE
HHHHCHHHHHCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KGIIDCINNFLESILEG
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1900939; 9141695; 9384377 [H]